FastQCFastQC Report
Wed 25 May 2016
SRR1294784_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294784_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2722299
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC39580.1453918177246511No Hit
CCCATGTACTCTGCGTTGATACCAC34600.12709845612109472No Hit
ATTCCATTCCATTCCATTCCATTCC31700.11644569534793936No Hit
GTATCAACGCAGAGTACTTTTTTTT27440.10079715710875256No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGTC658.042682E-411.68955512
CCGTGCA1157.096969E-811.5633469
GCGCGTC600.00588466111.0815399
CGGGACT700.001490372710.8585844
TAGACAG3350.010.2095595
TAATCGG750.002658706910.12871116
AATCGGA750.00265951910.12833717
GTCTTAC2350.010.1073531
TTAGACT2401.8189894E-129.5012614
GTCTAGC1901.0513759E-99.5009121
TCTAGTA2054.2200554E-109.2693532
GGAACGA1356.890652E-69.14397415
TGCACCG1054.4915464E-49.0481545
GCGTGCG1054.5014772E-49.0461559
GAACGAA1054.5147486E-49.0434916
TGCGGGA950.00182247419.001032
TAGACTG3200.08.9067775
CTGGACC2900.08.8460024
GTATAAA5200.08.7700731
GTCCTAG3250.08.7700721
Produced by FastQC (version 0.11.5)