FastQCFastQC Report
Wed 25 May 2016
SRR1294784_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294784_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2722299
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT92690.3404842745047476No Hit
GGTATCAACGCAGAGTACTTTTTTT60170.221026419214054No Hit
TATCAACGCAGAGTACTTTTTTTTT55390.20346773076726693No Hit
GTACATGGGAAGCAGTGGTATCAAC39960.14678769672251285No Hit
CCCATGTACTCTGCGTTGATACCAC36750.13499619255636505No Hit
ATTCCATTCCATTCCATTCCATTCC31960.11740077045173951No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28240.1037358497358299No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCCA400.005321949714.23319410
TCTAACG500.001465929113.3406712
ATAACCG657.8298623E-411.7269815
ACCGTGT1553.2014214E-1011.0192478
CTTAGAC2250.011.0103323
GCACCGT700.001500563510.8497196
GCGGGAC909.238591E-510.5868583
AGGGTCG750.002584386810.16338355
GTCTAGG3200.010.1272331
CTCGATT1651.0430085E-89.77633613
GTCTTAC2750.09.7048121
TAGGACC1502.538527E-79.52817254
CGCGATC1401.076156E-69.4889717
GTCGCCT800.0045614719.48879612
AGACCGC1002.789406E-49.48879614
TTAGGAC2201.5825208E-109.0950743
TAGCCCG850.00725925578.9676915
ACTCGAT1804.3031832E-88.9616412
GTCCTAG3300.08.9538451
TGGCGCG1701.7167622E-78.93063114
Produced by FastQC (version 0.11.5)