FastQCFastQC Report
Wed 25 May 2016
SRR1294783_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294783_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3769912
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC96500.25597414475457253No Hit
CCCATGTACTCTGCGTTGATACCAC92370.245018981875439No Hit
ATTCCATTCCATTCCATTCCATTCC66840.17729856824244172No Hit
GAATGGAATGGAATGGAATGGAATG51900.13766899598717425No Hit
GAGTACATGGGAAGCAGTGGTATCA40440.10727040843393691No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGACT655.446625E-513.1563864
CGTCCGC701.0942008E-412.21128610
ACGGATC855.3413554E-511.1735778
GACGAAG803.792629E-410.68160916
GACGCAA856.6124956E-410.05635410
ACCGTCT951.6495358E-49.9974128
CACCGTC1151.0303846E-59.9106097
TGCACCG1002.7529337E-49.5014565
CGTATAC800.0045157249.5005713
AGACGAA1201.7127486E-59.4948915
CGAATTA1201.7127486E-59.4948915
CCGAATT1106.8840134E-59.494637514
TAGGACG1151.1090683E-49.0887134
AGGACGG1151.10955036E-49.0883495
GTGCGCC1054.5098652E-49.04467611
GCACCGT1054.5164602E-49.0433536
TGTGCGC950.0018287948.99778910
CCTATAC3700.08.9870273
GTCTAGC1804.2164174E-88.9732391
GCGGGAC850.0074351838.9417143
Produced by FastQC (version 0.11.5)