FastQCFastQC Report
Wed 25 May 2016
SRR1294783_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294783_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3769912
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT112510.2984419795475332No Hit
GTACATGGGAAGCAGTGGTATCAAC96890.25700865166083453No Hit
CCCATGTACTCTGCGTTGATACCAC95140.2523666334917101No Hit
GGTATCAACGCAGAGTACTTTTTTT66730.17700678424323962No Hit
TATCAACGCAGAGTACTTTTTTTTT66170.17552133842911982No Hit
ATTCCATTCCATTCCATTCCATTCC62900.16684739590738457No Hit
GAATGGAATGGAATGGAATGGAATG53880.1429211079728121No Hit
GAGTACATGGGAAGCAGTGGTATCA42060.11156759096764063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCG504.4228927E-617.1571795
ACACCGT1102.7866918E-912.94370656
TCTGTCG2650.011.0987558
GGACCGA600.00591502711.07405956
GTTCGAT700.001508376110.84316711
ACCGTCT803.8190058E-410.67368
AGACCGT1252.269062E-710.6310996
TAACGCT909.20549E-510.5908524
GTCTTAC3450.010.224791
GACCGTG750.002676050910.120967
TGCGCGC750.002677817810.12015510
CTGTCGC2900.09.8148069
GTCTCGC1951.5279511E-109.7783851
TACCGCT800.00456372839.48847
AATCGGA800.00456522649.48802317
TAATCGG800.00456522649.48802316
CGAATTA1002.7927003E-49.48789715
CTCGCAT900.00112749189.48777213
GGAACGA1951.724402E-99.24461715
GTATTAA8150.09.0075141
Produced by FastQC (version 0.11.5)