FastQCFastQC Report
Wed 25 May 2016
SRR1294782_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294782_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4041787
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC218680.5410478088033832No Hit
CCCATGTACTCTGCGTTGATACCAC198280.4905750847335597No Hit
GAGTACATGGGAAGCAGTGGTATCA94970.23497032377015414No Hit
GCGTTGATACCACTGCTTCCCATGT71330.17648134352453507No Hit
CATGTACTCTGCGTTGATACCACTG67380.16670843861885845No Hit
GTATCAACGCAGAGTACATGGGAAG66320.16408583628974016No Hit
ACGCAGAGTACATGGGAAGCAGTGG51460.12731992061927064No Hit
ATTCCATTCCATTCCATTCCATTCC50860.12583542873486406No Hit
TATCAACGCAGAGTACATGGGAAGC50090.12393033081654228No Hit
GGTATCAACGCAGAGTACATGGGAA45840.11341517996866236No Hit
ACTCTGCGTTGATACCACTGCTTCC43200.10688341567727344No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCT658.0469466E-411.6890748
CCTGCGC1053.4816076E-610.8541388
ATACCGA1002.4132676E-510.4451676
CTGCGCA951.6501057E-49.9971089
GTCTACG800.00451268639.5013771
ACAGCGC1201.707558E-59.4973728
TGTACGG800.00452887279.49725410
GACCGCC1106.867355E-59.49678211
TCTGTCG3200.09.2005798
TCAGGAC5700.09.16799453
TAGACTG5700.08.8351615
TTAGACT4250.08.7197764
CTGTGCG1201.7705686E-48.7058159
CTGTCGC3650.08.5865589
GACAGCG1000.00291377128.5476347
CACACCG1356.786683E-58.4461925
ACCGCCT1356.830117E-58.44158512
GTAGGAC2502.3464963E-108.3612123
GTATTAG5700.08.334541
CCTACAC3800.08.2511963
Produced by FastQC (version 0.11.5)