FastQCFastQC Report
Wed 25 May 2016
SRR1294782_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294782_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4041787
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC217120.5371881299039262No Hit
CCCATGTACTCTGCGTTGATACCAC208190.5150939423576749No Hit
GTATCAACGCAGAGTACTTTTTTTT116540.28833780701457057No Hit
GAGTACATGGGAAGCAGTGGTATCA96760.23939905789196708No Hit
GCGTTGATACCACTGCTTCCCATGT73250.1812317175546361No Hit
GGTATCAACGCAGAGTACTTTTTTT71520.1769514326212638No Hit
CATGTACTCTGCGTTGATACCACTG70140.17353710128712868No Hit
TATCAACGCAGAGTACTTTTTTTTT69350.17158252030599336No Hit
GTATCAACGCAGAGTACATGGGAAG67060.1659167096138416No Hit
ACGCAGAGTACATGGGAAGCAGTGG55150.1364495457083711No Hit
TATCAACGCAGAGTACATGGGAAGC51670.12783949277881293No Hit
ATTCCATTCCATTCCATTCCATTCC49740.12306437721730512No Hit
ACTCTGCGTTGATACCACTGCTTCC46830.11586459157793322No Hit
GGTATCAACGCAGAGTACATGGGAA46110.11408320131664534No Hit
GAATGGAATGGAATGGAATGGAATG41400.10242994002405371No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCCGT500.001510865313.2875866
CGACCGC550.003101206212.07454210
TGGACGG600.00572695311.1222675
ACCCGTG1054.166262E-59.939777
ATAGGCG1252.4600267E-69.9147065
CCTAGAC3300.09.5333723
ACACCGT1401.0733693E-69.4911336
ATCGACC800.00456885069.487148
GTATTAG5300.09.3567521
CTAGGAC3600.09.2685563
TAGACTG5150.09.0705855
CTTATAC4750.09.0316153
CACCGTC950.00184594468.9885967
CCGCAAT950.00184719168.98792914
CTAATAC6400.08.9375353
TAGGACC2800.08.8524174
CTAGCAC4350.08.7663193
TACATCG1106.9034536E-48.66675
GTATTAA7200.08.6095121
TAATACT8800.08.5583674
Produced by FastQC (version 0.11.5)