FastQCFastQC Report
Wed 25 May 2016
SRR1294781_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294781_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2541235
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC33620.13229787878728255No Hit
CCCATGTACTCTGCGTTGATACCAC32300.12710355398064327No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCGC250.00603802518.99731810
GTACGGT1006.6029315E-1014.25108054
AGTACGG1900.013.9996813
GTACGGG707.2611892E-613.5724564
CGTATCC550.003073973812.08848812
TCGTCTA550.003081699112.08443514
GTATTAG4250.012.0710271
TCGTGGG1501.4551915E-1112.03092412
TACGGTC802.8658093E-511.87595
CGTCCGC802.8721784E-511.87332410
TCGTCCG752.0779016E-411.3981679
GGTACGA600.005877530711.0832992
GTACCGT1106.0791135E-610.3593146
GGGTACG750.002647758710.1337011
GGACCGA856.6213467E-410.0546286
GTCCTAG2300.09.91340351
GTCCTAA2151.0913936E-119.7212831
ACCGGAC800.0045179599.49978353
GTTAGAC800.0045179599.49978353
GCGGGAC800.0045179599.49978353
Produced by FastQC (version 0.11.5)