FastQCFastQC Report
Wed 25 May 2016
SRR1294781_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294781_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2541235
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT67230.26455640662906027No Hit
GGTATCAACGCAGAGTACTTTTTTT41990.16523462017483626No Hit
TATCAACGCAGAGTACTTTTTTTTT37840.14890397779032638No Hit
GTACATGGGAAGCAGTGGTATCAAC35790.1408370339618335No Hit
CCCATGTACTCTGCGTTGATACCAC31990.12588367466999315No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG451.6887043E-618.977029
CCGTCGT250.00607542918.97701813
AGTGCGC751.4979803E-512.6513458
GTCGCGC658.1084005E-411.67816458
CGCTATC752.015018E-411.4359392
TGTCCCG1001.9592917E-611.38621112
CGACCGC700.001507105110.8440110
GTCGGCC1053.5190897E-610.8440112
CGCGCTG1002.4322846E-510.43736110
AGTCGCG750.002674410810.1214747
TCGCGCT951.6649629E-49.9879059
GCTAGAC1159.897129E-69.9439023
ACATCGT1151.0418369E-59.9010538
GTATTAG3600.09.532211
TCGCTAT900.00108005999.532211
TCTAACC1401.0152016E-69.52957253
GTCCGCA800.00456243439.48850911
CATCGTA800.00456243439.4885099
TTAGGCT2355.456968E-129.3268164
AACAATC1554.3354157E-79.182797
Produced by FastQC (version 0.11.5)