FastQCFastQC Report
Wed 25 May 2016
SRR1294780_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294780_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3181209
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC42090.13230818849060216No Hit
CCCATGTACTCTGCGTTGATACCAC38690.12162042795679252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC1450.013.10110210
ACGCTCG701.09341796E-412.21209910
AGCGCGC1302.6266207E-911.69021310
TCGTCGC1201.2799865E-711.08134710
ACCCGTG600.00588785811.0808257
GTCCTAA2500.011.0208031
TCTCACG2800.010.90087119
GAACCGC1158.880161E-710.7339956
GCGGGAC1605.456968E-1010.6874393
GCGCAAG1502.4450856E-810.1340731
TCGTCCG1701.5661499E-910.0568649
TATACCG951.6454076E-49.9997845
GACAATG6750.09.9903327
ATCGTCG1054.1166782E-59.9504429
ACCGAGT1252.5785648E-69.8780758
GCGCGCG1356.466107E-79.84993311
AGCCGAA1554.0972736E-89.80191616
TGGACCG1651.0237272E-89.7876675
TCGTTCA2251.8189894E-129.70921913
CGTCCGA800.0043702789.53826319
Produced by FastQC (version 0.11.5)