FastQCFastQC Report
Wed 25 May 2016
SRR1294780_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294780_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3181209
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT108100.3398079157955356No Hit
GGTATCAACGCAGAGTACTTTTTTT65950.2073111197661015No Hit
TATCAACGCAGAGTACTTTTTTTTT64790.2036647073486841No Hit
GTACATGGGAAGCAGTGGTATCAAC43370.13633181598568342No Hit
CCCATGTACTCTGCGTTGATACCAC39100.12290924613881074No Hit
ACGCAGAGTACTTTTTTTTTTTTTT32420.10191094014885536No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG400.00516838314.2962535
GTCGCGC701.1047184E-412.1986558
CGTCCGC1603.6379788E-1211.85980310
CGAATTA658.113093E-411.67752815
ACCGTCC951.3807816E-510.9859238
TCGTCTA2000.010.91119114
ACGCGTG909.636524E-510.5430417
CGTGCGT909.6457414E-510.542047510
TCGTCCG1659.513315E-1010.3503739
ATCGTCC1856.184564E-1110.2571278
TCGACCG951.6664437E-49.9872039
TAGACGA1159.884077E-69.945224
CTAGGAC3150.09.6821183
GGTATCA31400.09.5335451
CTACACT4400.09.5308364
GTACGAC1002.6685806E-49.5308363
GAACGTC2002.6921043E-109.49351219
ATGTCGC800.00454493579.4930656
AGACCGT2401.8189894E-129.4930656
TGATCGT1106.9372254E-59.48784310
Produced by FastQC (version 0.11.5)