FastQCFastQC Report
Wed 25 May 2016
SRR1294779_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294779_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2686296
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC53480.19908453871055162No Hit
CCCATGTACTCTGCGTTGATACCAC47150.1755204936462698No Hit
GTATCAACGCAGAGTACTTTTTTTT32600.1213566933800296No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCCTA500.001494401713.30665316
ACCGCGG604.1093116E-412.6609728
TGATCGG550.003079802312.08547210
TGATCGC550.003079802312.08547210
AGACCGT951.0461317E-611.9948266
AACTGCG658.057102E-411.687057
TGCACCG855.3342217E-511.1747735
TAGACCG600.00588419511.081655
CGTTCGA600.005897255611.0783510
AATGCCG600.005897255611.0783510
TCGTTCG700.001497623510.8522619
TCGAGTC700.001498088610.85185713
GTCTAGC1909.094947E-1210.50270751
TAGACTG4050.010.319425
ACGTGTC1356.4870983E-79.8474218
CTAGCAC3150.09.6507643
GTCTAGG4500.09.502451
GTGCTAC2303.6379788E-129.502451
GTCCTAA2401.8189894E-129.5024491
CCAGTAC5000.09.4999713
Produced by FastQC (version 0.11.5)