##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294779_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2686296
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23533780342896	33.0	33.0	33.0	27.0	33.0
2	31.246540217459284	33.0	33.0	33.0	27.0	33.0
3	31.219524579569786	33.0	33.0	33.0	27.0	33.0
4	34.77855567666408	37.0	37.0	37.0	27.0	37.0
5	34.946206598230425	37.0	37.0	37.0	33.0	37.0
6	35.118733378600126	37.0	37.0	37.0	33.0	37.0
7	35.10406262005378	37.0	37.0	37.0	33.0	37.0
8	34.9883508742149	37.0	37.0	37.0	33.0	37.0
9	34.72511071006323	37.0	37.0	37.0	27.0	37.0
10	34.814595264259786	37.0	37.0	37.0	33.0	37.0
11	35.02641294928035	37.0	37.0	37.0	33.0	37.0
12	34.9545247433641	37.0	37.0	37.0	33.0	37.0
13	34.881946367786725	37.0	37.0	37.0	33.0	37.0
14	36.09984007719179	40.0	37.0	40.0	27.0	40.0
15	36.413876579498314	40.0	37.0	40.0	27.0	40.0
16	36.441758465932274	40.0	37.0	40.0	33.0	40.0
17	36.389394914037766	40.0	37.0	40.0	27.0	40.0
18	36.393541143641656	40.0	37.0	40.0	27.0	40.0
19	36.48568809989666	40.0	37.0	40.0	33.0	40.0
20	36.37566746181359	40.0	37.0	40.0	27.0	40.0
21	36.327464657655	40.0	37.0	40.0	27.0	40.0
22	36.19327877493768	40.0	37.0	40.0	27.0	40.0
23	35.81460605979386	37.0	37.0	40.0	27.0	40.0
24	36.12144678025057	40.0	37.0	40.0	27.0	40.0
25	35.012628541307436	37.0	33.0	40.0	27.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5917392341645353
1101	2	0.6425603936338256
1101	3	0.5295462735120182
1101	4	0.7101092860211864
1101	5	0.6607492974770182
1101	6	0.5445720580652704
1101	7	0.5319003496386756
1101	8	0.629147154435735
1101	9	0.915923943986904
1101	10	0.8202788335387581
1101	11	0.6269774721371135
1101	12	0.5708915446354439
1101	13	0.6418683328889756
1101	14	1.3488229081668095
1101	15	1.1510412785082096
1101	16	1.0399579319236167
1101	17	1.054130087042779
1101	18	1.1486533814132684
1101	19	0.8976824731482509
1101	20	1.1894027377020393
1101	21	1.0871042050415767
1101	22	1.1969371405351055
1101	23	1.5060912473462125
1101	24	1.3367504328859
1101	25	1.2754706954927997
1102	1	0.39073366826699996
1102	2	0.5654532589969037
1102	3	0.4879406572978482
1102	4	0.3594993191236142
1102	5	0.3782809559071936
1102	6	0.2918146576134788
1102	7	0.41160565227947643
1102	8	0.5083123398728873
1102	9	0.6597758554967896
1102	10	0.6495480564825655
1102	11	0.430545220258864
1102	12	0.3754998000271357
1102	13	0.4760838818922295
1102	14	1.1474352081909132
1102	15	0.8620840609995497
1102	16	0.6154732047935241
1102	17	0.8246133924569463
1102	18	0.6993067374838375
1102	19	0.8209472268221489
1102	20	0.9151061559148417
1102	21	0.9244749216331982
1102	22	0.9236162863263004
1102	23	1.1847720789958203
1102	24	1.050948845677695
1102	25	0.9487266906879981
1103	1	0.31207346428729466
1103	2	0.5238542729493822
1103	3	0.25990018793004666
1103	4	0.16532002957026748
1103	5	0.3217255538813646
1103	6	0.2956205234115288
1103	7	0.2768278619445539
1103	8	0.4114109538656905
1103	9	0.56513738124535
1103	10	0.5833084684690135
1103	11	0.3975316479074493
1103	12	0.45795333453384757
1103	13	0.4289005966720225
1103	14	1.1402971260768808
1103	15	0.8158081718911561
1103	16	0.5031536979297755
1103	17	0.5615535761124448
1103	18	0.742288738015958
1103	19	0.7058366860768075
1103	20	0.8524137214562586
1103	21	0.8695346006803817
1103	22	0.834401909171099
1103	23	1.2208541019339236
1103	24	0.8454216574623743
1103	25	0.8883013026557265
1104	1	0.30176528788613766
1104	2	0.49070982817870856
1104	3	0.32677533629382793
1104	4	0.2831219754122358
1104	5	0.23282875865417907
1104	6	0.13843906392834526
1104	7	0.18605235186578994
1104	8	0.36414390827049203
1104	9	0.4727621809722393
1104	10	0.5263314031828088
1104	11	0.37499992708332286
1104	12	0.3319176665854684
1104	13	0.32746822532129727
1104	14	0.7469276270029042
1104	15	0.6837170547965528
1104	16	0.5746055031367874
1104	17	0.6353682634407534
1104	18	0.5618584221212828
1104	19	0.5654297130702375
1104	20	0.6466800526024912
1104	21	0.7719325815704536
1104	22	0.8441382193559406
1104	23	1.243837174885826
1104	24	0.9925513208346644
1104	25	0.865508212104551
1105	1	0.2750034061099882
1105	2	0.29425285244152377
1105	3	0.3138665904168967
1105	4	0.35228600378747643
1105	5	0.3707619471772574
1105	6	0.2939934837171876
1105	7	0.20708978011136736
1105	8	0.31066576468118257
1105	9	-1.5960221227317817
1105	10	-0.17171744425059643
1105	11	0.02254009299991111
1105	12	0.2683927199428453
1105	13	0.2619778343809287
1105	14	-1.5666274647812486
1105	15	-0.22761837024047793
1105	16	-0.06581359478050075
1105	17	0.15518862300556435
1105	18	0.15167968083474648
1105	19	0.27154912947543863
1105	20	0.34114233804906036
1105	21	0.41857397742774793
1105	22	0.5119860834046648
1105	23	0.9275825549180965
1105	24	0.6613954415855972
1105	25	0.3545589477501139
1106	1	0.07828444361302189
1106	2	0.13252428016425455
1106	3	0.12713576220958345
1106	4	-0.9891763205751261
1106	5	-0.18475464474667547
1106	6	-0.13564362464613566
1106	7	0.027548063067612816
1106	8	0.24206294806019457
1106	9	0.44954644389361675
1106	10	0.46654730399242794
1106	11	0.36249144198185235
1106	12	0.15508555860932205
1106	13	0.16704142591789406
1106	14	0.6787482318676155
1106	15	0.43311961592850423
1106	16	0.2460246590493611
1106	17	0.1040095503292946
1106	18	0.2660488152159246
1106	19	0.21256033205474978
1106	20	0.45567781716525246
1106	21	0.549355107255316
1106	22	0.5490286153128281
1106	23	0.8586391490578222
1106	24	0.49310183991700995
1106	25	0.4483600373402439
1107	1	0.30104014803852763
1107	2	0.3260821631576434
1107	3	0.29887583939951057
1107	4	0.4432136077211908
1107	5	0.23912380050771986
1107	6	0.19495615095812013
1107	7	0.22998838365303698
1107	8	0.3328761334809158
1107	9	0.3819408923813299
1107	10	0.4552608717167388
1107	11	0.2345777957182804
1107	12	0.2683227037640279
1107	13	0.24555296648952663
1107	14	0.7168952104110531
1107	15	0.45061200949238156
1107	16	0.5523254547001741
1107	17	0.5255004379596571
1107	18	0.562188071180465
1107	19	0.5646021772222767
1107	20	0.6847720253506182
1107	21	0.5988212427658723
1107	22	0.6755765660635191
1107	23	0.9664519151093742
1107	24	0.7840732843363298
1107	25	0.5837967763650909
1108	1	0.4128154482190496
1108	2	0.38631840551742513
1108	3	0.40936849524320706
1108	4	0.6252257481575327
1108	5	0.5251362849965133
1108	6	0.3709538768553031
1108	7	0.30270890501837755
1108	8	0.44490800448642176
1108	9	0.32781367898594027
1108	10	0.5449165360474169
1108	11	0.37833549887444207
1108	12	0.3948668751692779
1108	13	0.3766560427170802
1108	14	-0.9128586137634613
1108	15	0.24314649078610273
1108	16	0.5063827037700293
1108	17	0.5604058182897163
1108	18	0.6284290778275974
1108	19	0.6238270312359973
1108	20	0.6865736854037223
1108	21	0.748015604081786
1108	22	0.8234759055457346
1108	23	1.0897989161362887
1108	24	0.950421387799615
1108	25	0.7357315994035929
1109	1	0.3306711932855855
1109	2	0.31708304112076746
1109	3	0.36501005170512
1109	4	0.5078345109804232
1109	5	0.45361906421621967
1109	6	0.23579804527288672
1109	7	0.35174332597993896
1109	8	0.3436257155233733
1109	9	0.49511067564890254
1109	10	0.4621481308235005
1109	11	0.26501153809497424
1109	12	0.19304283199373629
1109	13	0.28528857680845476
1109	14	0.7035268762872136
1109	15	0.3708083990792659
1109	16	0.3884795809586734
1109	17	0.35076496219446085
1109	18	0.4498499981917874
1109	19	0.3290818411070475
1109	20	0.5236725194992147
1109	21	0.5178514298964814
1109	22	0.5805006861661823
1109	23	1.073582338045405
1109	24	0.7707907282190121
1109	25	0.6148179298942651
1110	1	0.3460438952056464
1110	2	0.17790493731890678
1110	3	0.28910439651094677
1110	4	0.4585921588562769
1110	5	0.34771325489497684
1110	6	0.19090026325208242
1110	7	0.17985889136335942
1110	8	0.20486352815200348
1110	9	0.35600102239854436
1110	10	0.33921309357008056
1110	11	0.11682935355228352
1110	12	0.031284432521758276
1110	13	0.0611901974999256
1110	14	0.4446357842878612
1110	15	0.29452785245401714
1110	16	0.29593681523000726
1110	17	0.2961125232695778
1110	18	0.3061015734611061
1110	19	0.3121346631736941
1110	20	0.41130119293659106
1110	21	0.5322557378387387
1110	22	0.533005409707549
1110	23	0.7008452860791934
1110	24	0.564257508395734
1110	25	0.4957451356792859
1111	1	0.19302461556539896
1111	2	0.12739387387419043
1111	3	0.31411728740529554
1111	4	0.4608001602017353
1111	5	0.3660508745810489
1111	6	0.3161523314213923
1111	7	0.2099813737966727
1111	8	0.3051185686097071
1111	9	0.3343431123185283
1111	10	0.2999552841361961
1111	11	0.08359461346751829
1111	12	0.23120203179536958
1111	13	0.18823282928207874
1111	14	-1.5967744779069477
1111	15	-0.43758322539381567
1111	16	0.00814289213537478
1111	17	0.20189109102287972
1111	18	0.3142206138582111
1111	19	0.20512668229118702
1111	20	0.3655005616368143
1111	21	0.529346682618069
1111	22	0.2830683958743023
1111	23	-1.9025262934426124
1111	24	-0.3685470924322516
1111	25	-0.027409951467767257
1112	1	-0.1732987687863634
1112	2	-0.27609912409529613
1112	3	-0.07112227436729768
1112	4	0.1476940885591702
1112	5	-0.7268940394777701
1112	6	-0.9188525506766609
1112	7	-0.9601801185048728
1112	8	-0.7858186762676027
1112	9	-0.6251674048863407
1112	10	-0.8402314331355498
1112	11	-0.8758102816792146
1112	12	-0.7732525838195059
1112	13	-0.8393910048772639
1112	14	-0.51380712925544
1112	15	-0.8127403966480813
1112	16	-0.8604727246490924
1112	17	-0.6855274304319465
1112	18	-0.9849707079614731
1112	19	-0.877778291965825
1112	20	-0.9880885109643245
1112	21	-1.8501934852700046
1112	22	-1.7980877576253604
1112	23	-1.309997545169196
1112	24	-1.6098954079463752
1112	25	-1.964239831841752
1113	1	-0.06344182553184297
1113	2	-0.22146031774052943
1113	3	-0.14211633839525817
1113	4	-0.026201497143006236
1113	5	-0.1206868414485669
1113	6	-0.180503451905345
1113	7	-0.12312333241747808
1113	8	-0.06698183847792194
1113	9	0.07097656802954333
1113	10	-0.21959529534768762
1113	11	-0.26584936828559336
1113	12	-0.18806462908968058
1113	13	-0.18374037133447985
1113	14	-0.12286351306982368
1113	15	-0.2811669616777337
1113	16	-0.15223706707995888
1113	17	-0.14223035234367387
1113	18	-0.1674623810483098
1113	19	-0.23710658746044544
1113	20	-0.8578647423487027
1113	21	-0.6747034249960251
1113	22	-0.20962277679871022
1113	23	-0.3330541992208893
1113	24	-0.11174651228891719
1113	25	-1.2896416503836718
1114	1	-0.9917136896336238
1114	2	-0.670350520002998
1114	3	-0.5230188410239123
1114	4	-1.6009628035361985
1114	5	-0.9188155660388873
1114	6	-0.6141333339501855
1114	7	-0.5049951470386773
1114	8	-0.4359894408316336
1114	9	-0.3848062375725405
1114	10	-0.39369707204962623
1114	11	-0.5111006857854932
1114	12	-0.6207360891000064
1114	13	-0.6125907248087685
1114	14	-0.692162342505064
1114	15	-1.3945099620514796
1114	16	-0.8470846367426503
1114	17	-0.9100992668527468
1114	18	-0.7822269967200199
1114	19	-0.7679861207358769
1114	20	-2.0563665801612814
1114	21	-6.747429948017448
1114	22	-7.832706686039895
1114	23	-8.134253521928937
1114	24	-5.8563309520190785
1114	25	-7.288850172463061
1115	1	-0.9478495442959662
1115	2	-1.0575363076728586
1115	3	-0.8407490899819408
1115	4	-0.7142508989381824
1115	5	-0.7570245964434932
1115	6	-0.8503703149719186
1115	7	-1.062989289265495
1115	8	-0.8462034592544256
1115	9	-2.8569839493272013
1115	10	-1.8065362564862397
1115	11	-1.302874457710125
1115	12	-1.182717124172477
1115	13	-1.4644921532879636
1115	14	-1.0496371396200388
1115	15	-1.951532326493762
1115	16	-1.41598972192795
1115	17	-3.2451000752446504
1115	18	-2.8155551008515687
1115	19	-2.2360179292232942
1115	20	-1.8643197692006623
1115	21	-1.394356804824298
1115	22	-0.9748682459641458
1115	23	-0.5413268710886072
1115	24	-1.4555399018512887
1115	25	-1.1588421845905401
1116	1	-1.6247453629094082
1116	2	-0.9130164613667944
1116	3	-0.7499841387844377
1116	4	-0.5406238892857544
1116	5	-0.5452830422836712
1116	6	-0.5048602749338116
1116	7	-0.8737309874061054
1116	8	-1.853265351607014
1116	9	-0.975815778808176
1116	10	-2.4929526063474867
1116	11	-1.1270898021213966
1116	12	-0.8337205558736116
1116	13	-0.9635751895218547
1116	14	-1.264135465088458
1116	15	-1.3923257083355907
1116	16	-0.9643159372704488
1116	17	-0.8280792571476994
1116	18	-1.3529554624880902
1116	19	-1.1353605758572414
1116	20	-1.1791170852461264
1116	21	-0.6857186247415115
1116	22	-0.34903300639504664
1116	23	-0.24580232172782956
1116	24	-0.37674029482668203
1116	25	-0.23804322150651558
1201	1	0.2718762391335545
1201	2	0.42025261991708973
1201	3	0.4207682023208754
1201	4	0.5244325706279014
1201	5	0.4613002730968816
1201	6	0.3246991953773275
1201	7	0.3958460451318828
1201	8	0.4955722532277633
1201	9	0.6677932210141861
1201	10	0.6355936973328937
1201	11	0.4805317507876481
1201	12	0.5165331137373101
1201	13	0.5243466850253853
1201	14	1.1783343836924516
1201	15	0.8877092901336994
1201	16	0.8648202254207789
1201	17	0.7812040301383334
1201	18	0.7027907494756391
1201	19	0.66864862604411
1201	20	0.8192665347647292
1201	21	0.8378814049908101
1201	22	1.0034701148687262
1201	23	0.7074511626860058
1201	24	0.8092750576336059
1201	25	0.7218666492460741
1202	1	-0.18075401180103512
1202	2	0.004455229259828997
1202	3	0.04374832827235764
1202	4	0.09902019370143478
1202	5	0.18509809719461856
1202	6	0.04278597513208382
1202	7	0.13560465451812576
1202	8	0.2331757002447148
1202	9	0.2496384475502751
1202	10	0.5126808271542771
1202	11	0.235193177847691
1202	12	0.14372802109652838
1202	13	0.18041380305013632
1202	14	0.531785931831017
1202	15	0.45698150628844303
1202	16	0.4312230992813113
1202	17	0.38122385084829347
1202	18	0.23622495999514115
1202	19	0.3908781413209468
1202	20	0.5334508614121063
1202	21	0.6200274635565393
1202	22	0.5827234372650665
1202	23	0.8270552948185497
1202	24	0.5003085102278035
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2211	7	0.03911141623724035
2211	8	0.13698479281040932
2211	9	0.3639984264929481
2211	10	0.409077522038487
2211	11	0.2230295381708416
2211	12	0.14338312464369096
2211	13	0.1584989581914229
2211	14	0.44238481380510564
2211	15	0.20849108976212705
2211	16	0.25761110618016403
2211	17	0.23867853803480443
2211	18	0.3218803046707137
2211	19	0.35494218576219794
2211	20	0.3128436779943726
2211	21	0.3150695137738353
2211	22	0.431789478838553
2211	23	0.5557602972206297
2211	24	0.5076182902855635
2211	25	0.47598788309647944
2212	1	-0.3048854594739083
2212	2	-0.26528364165674034
2212	3	-0.1590037841753933
2212	4	-0.44202662559322903
2212	5	-0.12114554618841566
2212	6	-0.433790910651922
2212	7	-0.5591383676584982
2212	8	-0.37043159648438717
2212	9	-0.16462184732756668
2212	10	-0.5189930399717966
2212	11	-0.41875704261386915
2212	12	-0.6656403103242639
2212	13	-0.5467872543351007
2212	14	-0.4823174800105505
2212	15	-0.576829394920459
2212	16	-0.4140856309174268
2212	17	-0.4312334628317842
2212	18	-0.41040001615982646
2212	19	-0.44215299016428133
2212	20	-0.5292830793889536
2212	21	-0.446194771185354
2212	22	-0.5857188814706547
2212	23	-0.025658369053921604
2212	24	-0.2553535119695809
2212	25	-0.9931489049177458
2213	1	-0.11572597085801561
2213	2	-1.3005963952508743
2213	3	-0.3791488711452118
2213	4	0.004197637761372164
2213	5	0.10895059318973921
2213	6	-0.10153404550690937
2213	7	0.024757834247601807
2213	8	0.13327038570259475
2213	9	0.08429109644686861
2213	10	-0.4047192803630253
2213	11	-0.10121371838731363
2213	12	0.0037944630034161264
2213	13	-0.38427790866303013
2213	14	-0.030066229185592874
2213	15	-0.1290698129595853
2213	16	0.02518862350917317
2213	17	0.22184465517491958
2213	18	0.07293239138532925
2213	19	0.1072303604185052
2213	20	0.30045574831633814
2213	21	-0.4081046530483974
2213	22	-0.887732268580578
2213	23	-0.07674070056739168
2213	24	0.07322914463387775
2213	25	-0.41138989034796936
2214	1	-0.27839373975447756
2214	2	-0.4792112348265718
2214	3	-0.49324594058021276
2214	4	-0.6114976264456686
2214	5	-0.3062198436082113
2214	6	-0.3167936061157661
2214	7	-0.5597431833837518
2214	8	-0.18740402289856206
2214	9	-0.24516468816020875
2214	10	-2.055246812424201
2214	11	-0.9612319274695054
2214	12	-0.5444196835664314
2214	13	-0.4078346116700402
2214	14	-0.9705905934890637
2214	15	-0.6770954505633
2214	16	-0.817625115892838
2214	17	-0.631749596858107
2214	18	-0.7368822866222402
2214	19	-1.6219931796050133
2214	20	-2.5401444519336067
2214	21	-1.888663296775384
2214	22	-2.2716224672000394
2214	23	-2.792186896582322
2214	24	-1.7991172818097212
2214	25	-2.150894759346251
2215	1	-0.09211994192873263
2215	2	-0.07432340721637232
2215	3	-0.45292739522259495
2215	4	-0.0789729595544344
2215	5	-0.030908814964270448
2215	6	0.028084982852583096
2215	7	-0.10145840997614641
2215	8	-0.1594160011151331
2215	9	-0.1361919771627953
2215	10	-0.18145653136685524
2215	11	-0.45417279744733463
2215	12	-0.19563923913114678
2215	13	-0.15597864341489043
2215	14	-0.0018730735220913175
2215	15	-0.44378567637602373
2215	16	-0.2866952475321298
2215	17	0.009865272092824284
2215	18	-0.07988871838544043
2215	19	-1.927766854372578
2215	20	-2.097683913226284
2215	21	-3.0618835661495467
2215	22	-2.14246217709627
2215	23	-3.1634866274718405
2215	24	-1.8531908675532947
2215	25	-1.2802377394657753
2216	1	-1.6701554849485802
2216	2	-1.2869050933551698
2216	3	-0.9323597003008786
2216	4	-1.1274624238854827
2216	5	-1.989181337110253
2216	6	-1.6907093599456218
2216	7	-1.253481274145841
2216	8	-5.319430212566196
2216	9	-4.098622670817836
2216	10	-2.0104789853591214
2216	11	-1.3133524124830274
2216	12	-1.3200163794464643
2216	13	-1.9326411380199175
2216	14	-3.553681025474937
2216	15	-2.126601384547925
2216	16	-2.9959728382393322
2216	17	-2.714101891623187
2216	18	-2.5645658053325064
2216	19	-1.6078860350954827
2216	20	-0.9968964093960366
2216	21	-0.8924881569569862
2216	22	-0.853659451684031
2216	23	-1.16700233830462
2216	24	-0.7215341239670678
2216	25	-0.5288894339035082
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	32.0
8	131.0
9	537.0
10	1069.0
11	2133.0
12	3651.0
13	5522.0
14	7627.0
15	9680.0
16	11108.0
17	12164.0
18	12609.0
19	13168.0
20	13793.0
21	14896.0
22	15914.0
23	17044.0
24	18226.0
25	20241.0
26	22154.0
27	25178.0
28	28613.0
29	34326.0
30	41537.0
31	51063.0
32	69069.0
33	99274.0
34	144298.0
35	229713.0
36	403473.0
37	1358051.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.44425192786549	19.907703753849866	12.846664827146551	23.801379491138093
2	15.867356094684162	19.865125183458197	39.82332910631751	24.44418961554013
3	19.21560509044252	24.25436723374833	28.856042973647106	27.673984702162045
4	12.412960859241686	16.07328694721	37.272504884170594	34.24124730937772
5	13.374709764281684	37.734441205950816	35.11107520139641	13.779773828371091
6	33.11257932978593	37.11392403178292	16.562953608897963	13.210543029533184
7	27.743203089460795	32.210994031182	21.812095027573303	18.233707851783905
8	24.99060973616802	37.2064402505609	19.849361182864268	17.95358883040681
9	24.238545941871177	16.97361588072703	21.122744320667096	37.6650938567347
10	14.647504157225196	30.392210512130685	33.2715752263254	21.688710104318712
11	33.823208607351035	24.777516117191844	22.224228314289523	19.1750469611676
12	21.292366453419003	26.79620360839614	30.89245728687177	21.018972651313085
13	27.64054279830305	22.73459289382733	25.986885699948033	23.637978607921593
14	20.59913493528022	22.563938959294163	27.372534362665295	29.46439174276032
15	23.265991548803964	30.601616307063352	23.157475838967898	22.97491630516479
16	22.40209036689751	30.39914348266174	25.175310947287777	22.023455203152974
17	20.991173872369036	29.673121688433863	27.574752562048882	21.760951877148223
18	21.44178694649316	29.115200600394008	28.329305392378036	21.1137070607348
19	21.812775218061862	28.431707949918827	28.40985616162729	21.345660670392018
20	22.602884806168827	28.142662662617944	27.981175461539575	21.27327706967365
21	22.68057791489991	27.952436287821858	27.69528882146209	21.67169697581614
22	21.997890596388977	27.786828782517954	28.201519202763166	22.013761418329906
23	22.088312117964083	28.082003204752066	28.010173839730356	21.819510837553494
24	22.061565150059934	27.934861108008906	27.98024018523896	22.023333556692204
25	22.408814136047518	27.799003074351553	27.4998880697251	22.292294719875834
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1713.0
1	1713.0
2	1971.0
3	2229.0
4	2229.0
5	2229.0
6	3271.5
7	4314.0
8	4314.0
9	4314.0
10	6857.0
11	9400.0
12	9400.0
13	9400.0
14	15772.0
15	22144.0
16	22144.0
17	22144.0
18	34740.5
19	47337.0
20	47337.0
21	47337.0
22	67163.0
23	86989.0
24	86989.0
25	86989.0
26	113552.5
27	140116.0
28	140116.0
29	140116.0
30	168204.5
31	196293.0
32	196293.0
33	196293.0
34	221631.0
35	246969.0
36	246969.0
37	246969.0
38	267015.0
39	287061.0
40	287061.0
41	287061.0
42	294787.5
43	302514.0
44	302514.0
45	302514.0
46	309536.0
47	316558.0
48	316558.0
49	316558.0
50	304634.0
51	292710.0
52	292710.0
53	292710.0
54	271224.0
55	249738.0
56	249738.0
57	249738.0
58	223462.0
59	197186.0
60	197186.0
61	197186.0
62	168686.5
63	140187.0
64	140187.0
65	140187.0
66	110829.0
67	81471.0
68	81471.0
69	81471.0
70	59711.5
71	37952.0
72	37952.0
73	37952.0
74	27062.5
75	16173.0
76	16173.0
77	16173.0
78	10666.0
79	5159.0
80	5159.0
81	5159.0
82	3349.5
83	1540.0
84	1540.0
85	1540.0
86	981.5
87	423.0
88	423.0
89	423.0
90	257.5
91	92.0
92	92.0
93	92.0
94	54.5
95	17.0
96	17.0
97	17.0
98	14.0
99	11.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030264721385878546
2	0.014815939866641651
3	0.020734870617385427
4	0.021553842167802803
5	0.02039983680130559
6	0.0074079699333208255
7	7.445195912885252E-5
8	1.1167793869327878E-4
9	7.817455708529514E-4
10	3.350338160798363E-4
11	0.0012656833051904928
12	0.0022707847534300016
13	0.0025313666103809855
14	8.561975299818038E-4
15	0.0018240729986568867
16	0.0025685925899454117
17	0.0024941406308165593
18	0.0022335587738655754
19	6.328416525952464E-4
20	5.583896934663939E-4
21	0.08312561236736384
22	0.0791052065744058
23	0.08029643792046744
24	0.0013401352643193452
25	0.22544053224216543
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2686296.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.28963736117305
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.94823756938513	64.59353038222744
2	9.073871825934907	13.481892948209385
3	1.9900128870336677	4.435120071653766
4	0.6821977421357466	2.0272089148750263
5	0.34334429840159597	1.2753461709140475
6	0.19381098906752814	0.8638888856662164
7	0.1309804037432705	0.6811340686855318
8	0.09632327853794972	0.5724657145618838
9	0.07175268626628165	0.4797432938170877
>10	0.412152788559782	5.834832611130391
>50	0.04055105313037476	2.0840567868057778
>100	0.01520574380065711	2.001088171051415
>500	6.033809044821019E-4	0.2844890070082096
>1k	9.05071356564495E-4	1.1854331192366072
>5k	5.028174203136083E-5	0.19976985415715692
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	5348	0.19908453871055162	No Hit
CCCATGTACTCTGCGTTGATACCAC	4715	0.1755204936462698	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3260	0.1213566933800296	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.722597956442626E-5	0.0
5	0.0	0.0	0.0	3.722597956442626E-5	0.0
6	0.0	0.0	0.0	3.722597956442626E-5	0.0
7	0.0	7.445195912885252E-5	0.0	7.445195912885252E-5	0.0
8	0.0	7.445195912885252E-5	0.0	7.445195912885252E-5	0.0
9	0.0	7.445195912885252E-5	0.0	1.1167793869327877E-4	0.0
10	0.0	7.445195912885252E-5	0.0	1.4890391825770504E-4	0.0
11	0.0	7.445195912885252E-5	0.0	1.4890391825770504E-4	0.0
12	0.0	1.1167793869327877E-4	0.0	2.2335587738655754E-4	0.0
13	0.0	1.1167793869327877E-4	0.0	2.605818569509838E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCCCTA	50	0.0014944017	13.306653	16
ACCGCGG	60	4.1093116E-4	12.660972	8
TGATCGG	55	0.0030798023	12.085472	10
TGATCGC	55	0.0030798023	12.085472	10
AGACCGT	95	1.0461317E-6	11.994826	6
AACTGCG	65	8.057102E-4	11.68705	7
TGCACCG	85	5.3342217E-5	11.174773	5
TAGACCG	60	0.005884195	11.08165	5
CGTTCGA	60	0.0058972556	11.07835	10
AATGCCG	60	0.0058972556	11.07835	10
TCGTTCG	70	0.0014976235	10.852261	9
TCGAGTC	70	0.0014980886	10.851857	13
GTCTAGC	190	9.094947E-12	10.5027075	1
TAGACTG	405	0.0	10.31942	5
ACGTGTC	135	6.4870983E-7	9.847421	8
CTAGCAC	315	0.0	9.650764	3
GTCTAGG	450	0.0	9.50245	1
GTGCTAC	230	3.6379788E-12	9.50245	1
GTCCTAA	240	1.8189894E-12	9.502449	1
CCAGTAC	500	0.0	9.499971	3
>>END_MODULE