FastQCFastQC Report
Wed 25 May 2016
SRR1294779_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294779_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2686296
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT101470.37773201464023326No Hit
GGTATCAACGCAGAGTACTTTTTTT81900.30488077263265106No Hit
TATCAACGCAGAGTACTTTTTTTTT67680.2519454296920369No Hit
GTACATGGGAAGCAGTGGTATCAAC57870.21542674373933476No Hit
CCCATGTACTCTGCGTTGATACCAC50400.18761893700470833No Hit
ACGCAGAGTACTTTTTTTTTTTTTT40720.15158418878634372No Hit
GAGTACATGGGAAGCAGTGGTATCA29370.10933270198071991No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCAG651.878343E-716.1041245
TAGGACG400.00522153414.2741114
ATACCGT500.001506015113.2929896
ACGGATC701.09819055E-412.2062588
TCTACGC1103.729292E-812.1113663
GGGCCGT658.062341E-411.6861446
CTAGCAC2300.011.58478453
GTCTTAG2850.011.0219011
AGAACCG1002.3557941E-510.4676815
CTATCGG1106.0970015E-610.35682412
TCCAACG856.6362694E-410.05221112
TATACTG6750.09.5865625
GTATACG800.0044437369.5189151
GTTAGAC1002.7101077E-49.5160733
ACGCCAG1002.7659736E-49.4967619
ACTATCG1201.7145932E-59.49375511
GTCTAAG2357.2759576E-129.3163841
TCTAGTA1951.6334525E-99.2722442
TAGGACT3200.09.2186974
TAGCACT2950.09.0322054
Produced by FastQC (version 0.11.5)