FastQCFastQC Report
Wed 25 May 2016
SRR1294778_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294778_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3257732
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1034023.1740486939993837No Hit
CCCATGTACTCTGCGTTGATACCAC972632.9856047090429785No Hit
GAGTACATGGGAAGCAGTGGTATCA489981.5040525126069302No Hit
CATGTACTCTGCGTTGATACCACTG382591.1744059977923291No Hit
GCGTTGATACCACTGCTTCCCATGT307220.9430487222398896No Hit
GCTTCCCATGTACTCTGCGTTGATA303840.9326734059155265No Hit
ACGCAGAGTACATGGGAAGCAGTGG264690.8124977745253446No Hit
GTATCAACGCAGAGTACATGGGAAG251030.770566762397889No Hit
ACTCTGCGTTGATACCACTGCTTCC205290.6301623337954135No Hit
TATCAACGCAGAGTACATGGGAAGC196790.6040705619737904No Hit
GGTATCAACGCAGAGTACATGGGAA168590.5175072719302877No Hit
CAGTGGTATCAACGCAGAGTACATG137440.42188860225457464No Hit
GTGGTATCAACGCAGAGTACATGGG129310.3969325899122457No Hit
GCAGTGGTATCAACGCAGAGTACAT114070.35015157784618256No Hit
ATACCACTGCTTCCCATGTACTCTG101620.3119348061780404No Hit
CATGGGAAGCAGTGGTATCAACGCA97920.3005772113850986No Hit
GTACATGGTAAGCAGTGGTATCAAC97750.30005537594866616No Hit
ACATGGGAAGCAGTGGTATCAACGC96930.2975382873729331No Hit
GTACTCTGCGTTGATACCACTGCTT96520.29627974308506655No Hit
GGGAAGCAGTGGTATCAACGCAGAG92580.28418543944069063No Hit
CTGCTTCCCATGTACTCTGCGTTGA91070.27955031291708465No Hit
AAGCAGTGGTATCAACGCAGAGTAC80270.24639841460255169No Hit
CTTCCCATGTACTCTGCGTTGATAC79350.24357436400538782No Hit
TACCACTGCTTCCCATGTACTCTGC79020.24256138933466598No Hit
GCAGAGTACATGGGAAGCAGTGGTA78920.2422544273132351No Hit
GTTGATACCACTGCTTCCCATGTAC78260.24022847797179142No Hit
GATACCACTGCTTCCCATGTACTCT76360.23439619956460508No Hit
CCACTGCTTCCCATGTACTCTGCGT76190.23387436412817258No Hit
ACCATGTACTCTGCGTTGATACCAC67290.206554744220826No Hit
GTACATGGGTAAGCAGTGGTATCAA66480.20406835184723607No Hit
CCATGTACTCTGCGTTGATACCACT66150.20305537717651423No Hit
ATGGGAAGCAGTGGTATCAACGCAG64320.19743797218432949No Hit
TTCCCATGTACTCTGCGTTGATACC56800.17435442817272875No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA51650.15854588406903944No Hit
TGATACCACTGCTTCCCATGTACTC50920.15630506131259417No Hit
GGTAAGCAGTGGTATCAACGCAGAG46060.14138670707105433No Hit
CCCCATGTACTCTGCGTTGATACCA45870.1408034792303357No Hit
GCTTACCATGTACTCTGCGTTGATA39310.12066677062447125No Hit
ATCAACGCAGAGTACATGGGAAGCA37240.11431265678085245No Hit
GAGTACATGGTAAGCAGTGGTATCA34430.10568702397864528No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACA400.00528482514.2483424
ACCGATG550.0030797912.0856058
CGCAAAA2150.011.9299442
TAACCCG600.00588511111.0815345
CCATCGA750.002658562310.1288889
AGGACCG856.606934E-410.057195
CTTAGAC2650.010.0367223
GTCTAGA2800.09.8411621
GCTAGAC1554.0745363E-89.8054623
TTATACT4900.09.692754
ACGCAAA2950.09.662861
CGTGCAC900.00111852599.49597910
CGTTCCA1606.686059E-89.49597810
GTACAAA6650.09.430371
CTACACT3850.09.3755324
TTAGACA3350.09.35712054
TCAGAAC2550.09.3127843
TATACTG6900.09.223145
GTCCTAC1651.0636177E-79.2138791
CCAGGAC4600.09.0860393
Produced by FastQC (version 0.11.5)