##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294778_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3257732
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.999792186711492	33.0	33.0	33.0	27.0	33.0
2	30.979428326209767	33.0	33.0	33.0	27.0	33.0
3	31.031794205293743	33.0	33.0	33.0	27.0	33.0
4	34.41831433647703	37.0	37.0	37.0	27.0	37.0
5	34.843160517808094	37.0	37.0	37.0	33.0	37.0
6	34.88264627047283	37.0	37.0	37.0	33.0	37.0
7	34.93806212420174	37.0	37.0	37.0	33.0	37.0
8	34.81396321121566	37.0	37.0	37.0	33.0	37.0
9	34.52662772751104	37.0	37.0	37.0	27.0	37.0
10	34.65738249800782	37.0	37.0	37.0	27.0	37.0
11	34.87825671356637	37.0	37.0	37.0	33.0	37.0
12	34.82374486299057	37.0	37.0	37.0	33.0	37.0
13	34.6844777286775	37.0	37.0	37.0	27.0	37.0
14	35.95515254170693	40.0	37.0	40.0	27.0	40.0
15	36.180041206581755	40.0	37.0	40.0	27.0	40.0
16	36.17485508322968	40.0	37.0	40.0	27.0	40.0
17	36.1731336402135	40.0	37.0	40.0	27.0	40.0
18	36.166516152955495	40.0	37.0	40.0	27.0	40.0
19	36.174564390195385	40.0	37.0	40.0	27.0	40.0
20	36.10574197018048	40.0	37.0	40.0	27.0	40.0
21	36.024423740197165	40.0	37.0	40.0	27.0	40.0
22	35.950788155686226	40.0	37.0	40.0	27.0	40.0
23	35.6226924130039	37.0	37.0	40.0	27.0	40.0
24	35.947083124087555	40.0	37.0	40.0	27.0	40.0
25	34.88956427354982	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.4988597554784846
1101	2	0.6266399510260143
1101	3	0.4785427913136786
1101	4	0.7326287131265303
1101	5	0.5470814904299317
1101	6	0.480428517870628
1101	7	0.5154378178429937
1101	8	0.5555660407438552
1101	9	0.778964022708557
1101	10	0.7289586986582819
1101	11	0.45582091806885217
1101	12	0.5497622963389688
1101	13	0.545832239029238
1101	14	1.204227345916955
1101	15	0.9323614159561586
1101	16	0.9523264151769553
1101	17	1.023718718063428
1101	18	0.9623950088938287
1101	19	1.0052605294973702
1101	20	1.0364795746335105
1101	21	1.0540078056184612
1101	22	1.1902930484603758
1101	23	1.4506624003091915
1101	24	1.2978493803599136
1101	25	1.1495017627571755
1102	1	0.2401496376251835
1102	2	0.5341495871416448
1102	3	0.351287491175448
1102	4	0.3496019160290871
1102	5	0.42115317474622316
1102	6	0.34160350748069845
1102	7	0.34079771717163254
1102	8	0.46505826607116774
1102	9	0.7934542060819112
1102	10	0.7640705957139247
1102	11	0.5608332449322972
1102	12	0.4774801142086247
1102	13	0.5344367561811723
1102	14	1.147520873964318
1102	15	0.8591337041231029
1102	16	0.6431301171819186
1102	17	0.826830415098101
1102	18	0.806233734693464
1102	19	0.808748628030024
1102	20	0.9748446861957376
1102	21	0.8305387284120371
1102	22	0.9412075336080719
1102	23	1.3313471680532913
1102	24	1.0434247293398187
1102	25	0.9442035711277725
1103	1	0.397014167478968
1103	2	0.5751236413700411
1103	3	0.4532161345439967
1103	4	0.2742097344917056
1103	5	0.4179913802198314
1103	6	0.4025765271644275
1103	7	0.4467167905673577
1103	8	0.4919028744199423
1103	9	0.7358188100490821
1103	10	0.6331771359922698
1103	11	0.5968280530829446
1103	12	0.576919808296374
1103	13	0.5872592591793904
1103	14	1.1762609159285802
1103	15	0.9479548839473964
1103	16	0.6589586070640294
1103	17	0.6866242712068455
1103	18	0.8746848604997481
1103	19	0.9347744247054806
1103	20	0.9987084583976937
1103	21	1.0236548334470115
1103	22	0.9219632206696176
1103	23	1.339089170001543
1103	24	1.0673163212302015
1103	25	1.0229929278365972
1104	1	0.26517400116700784
1104	2	0.4235153863356871
1104	3	0.3256467287315843
1104	4	0.3341364796529902
1104	5	0.339558771779366
1104	6	0.12778701148677385
1104	7	0.2941692880537943
1104	8	0.3498411402911117
1104	9	0.5246294991991718
1104	10	0.5187229151627832
1104	11	0.3668640000114607
1104	12	0.3056538741780983
1104	13	0.42702782424537844
1104	14	0.8853196950259417
1104	15	0.6009525311503197
1104	16	0.5667906313031068
1104	17	0.6561496798841304
1104	18	0.5402045598588927
1104	19	0.6841670866325273
1104	20	0.7175399365428774
1104	21	0.6323355148224863
1104	22	0.7197437147399839
1104	23	1.1299538356849013
1104	24	0.9013719105267626
1104	25	0.8068583199439274
1105	1	0.21767616173148596
1105	2	0.2750140670559169
1105	3	0.216917797061015
1105	4	0.34853803803913763
1105	5	0.3362908813761223
1105	6	0.2284053490485789
1105	7	0.2607349190616972
1105	8	0.3275573520517199
1105	9	-1.7272809321116256
1105	10	-0.27850951100418797
1105	11	0.056290873130016905
1105	12	0.19064436032645204
1105	13	0.3114689802817523
1105	14	-1.5312527513258303
1105	15	-0.224162144964275
1105	16	-0.01216575535227804
1105	17	0.23918627225238254
1105	18	0.1193232504956967
1105	19	0.28345111550212465
1105	20	0.3892564185728986
1105	21	0.622602801891972
1105	22	0.6414018812048425
1105	23	0.9693839573948821
1105	24	0.8258892560792646
1105	25	0.5248805004554242
1106	1	0.021833698832381288
1106	2	0.08500588212427829
1106	3	0.04529132156696036
1106	4	-1.1119176017692212
1106	5	-0.307200719128204
1106	6	-0.042265545902075985
1106	7	0.05436593664254019
1106	8	0.19416000159812796
1106	9	0.35037003846963444
1106	10	0.4194464824753652
1106	11	0.20856863574895357
1106	12	0.1754661633887622
1106	13	0.2609880106589344
1106	14	0.6501906664918025
1106	15	0.38878044527331923
1106	16	0.20239342951269634
1106	17	0.2362553659193125
1106	18	0.17611717174502672
1106	19	0.1947803621464459
1106	20	0.2533384414464308
1106	21	0.48005204053647077
1106	22	0.4399197385532645
1106	23	0.6314530040462074
1106	24	0.3815841717866135
1106	25	0.27690283552352923
1107	1	0.27917213011962616
1107	2	0.2976658577890774
1107	3	0.2838398048214401
1107	4	0.4837475338375441
1107	5	0.34228658652457966
1107	6	0.23144058871563544
1107	7	0.21198728302413627
1107	8	0.2651251516320272
1107	9	0.48713979523341067
1107	10	0.42450144062540573
1107	11	0.28213462824915325
1107	12	0.2825023991412152
1107	13	0.2496577264163804
1107	14	0.7532172721967001
1107	15	0.5116534017783962
1107	16	0.6073102399871431
1107	17	0.5077009158759012
1107	18	0.5420397862960229
1107	19	0.44662590995825013
1107	20	0.6144085988474828
1107	21	0.6018288056589114
1107	22	0.5147637774545046
1107	23	0.8777925391418009
1107	24	0.6937455018929626
1107	25	0.699609115815683
1108	1	0.3995502976737626
1108	2	0.3388319640260029
1108	3	0.44731164923402744
1108	4	0.6626911884041036
1108	5	0.5343051851472893
1108	6	0.38138123090293163
1108	7	0.3393012028237621
1108	8	0.45302015467961354
1108	9	0.21445552312673044
1108	10	0.541974477428198
1108	11	0.3254450384437533
1108	12	0.28355889683518143
1108	13	0.3510493400020209
1108	14	-0.9349606975139295
1108	15	0.03882481524613013
1108	16	0.39755252930021356
1108	17	0.4832477809507125
1108	18	0.5948429262886279
1108	19	0.6169133159671176
1108	20	0.6791894079313465
1108	21	0.6544735947090956
1108	22	0.7637314749090365
1108	23	1.0187582324672206
1108	24	0.9098635152167063
1108	25	0.7942510680620245
1109	1	0.35866709206095493
1109	2	0.25311330151073363
1109	3	0.3495003232766969
1109	4	0.3540651701823876
1109	5	0.46451348257717484
1109	6	0.24757186575244816
1109	7	0.2761187980013986
1109	8	0.35191643489116586
1109	9	0.3990953589091575
1109	10	0.49749977834694903
1109	11	0.350867741676403
1109	12	0.3401660882474644
1109	13	0.28727984203750623
1109	14	0.6735803013486503
1109	15	0.4235872014561437
1109	16	0.3774868116334176
1109	17	0.3834484572758896
1109	18	0.3885710251868275
1109	19	0.3037548467751492
1109	20	0.4122895124821824
1109	21	0.5045067216215529
1109	22	0.5434807826906081
1109	23	0.9068164592391312
1109	24	0.6935643083477601
1109	25	0.5272148352621713
1110	1	0.322027020592202
1110	2	0.20020736029677266
1110	3	0.3363264489936739
1110	4	0.4969604396760303
1110	5	0.444736971949375
1110	6	0.296480677677863
1110	7	0.15216928962942688
1110	8	0.2886013612263909
1110	9	0.3685796587714165
1110	10	0.4103138792218033
1110	11	0.19106013167451863
1110	12	0.21176772470250427
1110	13	0.11889598156604109
1110	14	0.6286390892580229
1110	15	0.41662385722680995
1110	16	0.3481175271246073
1110	17	0.3758109817825286
1110	18	0.49504974996297335
1110	19	0.3895745414256453
1110	20	0.4752365893466006
1110	21	0.5792039045490469
1110	22	0.6171554615578003
1110	23	0.7882325273736726
1110	24	0.6741402822123206
1110	25	0.5437621692944887
1111	1	0.16791293518806327
1111	2	0.09717920747345232
1111	3	0.19774044132385526
1111	4	0.3105338369445363
1111	5	0.19997082094239005
1111	6	0.12893561836119005
1111	7	0.1121023448270364
1111	8	0.19982009484902363
1111	9	0.17624030235969457
1111	10	0.041212793771613576
1111	11	0.041797178002703106
1111	12	-0.00813990763480632
1111	13	0.12104012295835531
1111	14	-1.5762493230255572
1111	15	-0.47323093611824163
1111	16	0.013667621864172474
1111	17	0.13060973324812863
1111	18	0.16500644954926003
1111	19	0.17912604802046417
1111	20	0.23007808238170924
1111	21	0.38916877017796025
1111	22	0.17454488367467036
1111	23	-1.7770707475443643
1111	24	-0.27783271996972303
1111	25	0.0065418894755566726
1112	1	-0.13869073567038015
1112	2	-0.29040824105279484
1112	3	-0.07895780964492616
1112	4	0.07784098178107968
1112	5	-0.6312418578863088
1112	6	-0.7472798534079743
1112	7	-0.8308974995851557
1112	8	-0.5295811748303478
1112	9	-0.43532758365891056
1112	10	-0.7572328704159048
1112	11	-0.6816844716679498
1112	12	-0.5474394325957235
1112	13	-0.7219449980913168
1112	14	-0.3665990300185058
1112	15	-0.785350262759323
1112	16	-0.6207704587205995
1112	17	-0.7194096134787031
1112	18	-0.8552692700479412
1112	19	-0.7940056004756073
1112	20	-0.815643206365209
1112	21	-1.731852342852548
1112	22	-1.590979034898993
1112	23	-1.184022773731101
1112	24	-1.486715106079835
1112	25	-1.7169761758831754
1113	1	-0.0346851808102393
1113	2	-0.20395535449493707
1113	3	-0.19531093449461068
1113	4	0.00489145003956537
1113	5	-0.1879954272197466
1113	6	-0.20130990904942792
1113	7	-0.1734208308285261
1113	8	-0.11586623155983489
1113	9	0.057842845367602536
1113	10	-0.1216560045988544
1113	11	-0.18146379359495768
1113	12	-0.04894396696813175
1113	13	-0.27654878879747
1113	14	-0.09965741518139737
1113	15	-0.16620665285560676
1113	16	-0.16783325172031027
1113	17	-0.18303330102536108
1113	18	-0.13329393670431955
1113	19	-0.29051834936414167
1113	20	-0.83576487880201
1113	21	-0.7654912628410742
1113	22	-0.1787047473432466
1113	23	-0.5517662018564025
1113	24	-0.17876482420042095
1113	25	-1.1893301211721905
1114	1	-1.0508894300448297
1114	2	-0.8330205948867615
1114	3	-0.5831343693895228
1114	4	-1.5728247112043903
1114	5	-0.8748001370236835
1114	6	-0.7258937357628952
1114	7	-0.6286601117429171
1114	8	-0.5545607490096103
1114	9	-0.5088965194741704
1114	10	-0.7181499023347016
1114	11	-0.7121659286521265
1114	12	-0.6601877729260863
1114	13	-0.707010700365835
1114	14	-0.7503466819443005
1114	15	-1.3612671511011598
1114	16	-0.8405503778460997
1114	17	-0.8763547297450955
1114	18	-0.86062378657914
1114	19	-0.7689641109074685
1114	20	-2.1471679220080944
1114	21	-6.810294795019001
1114	22	-7.781349179069032
1114	23	-7.95968673718788
1114	24	-5.815044799928572
1114	25	-7.348925969699025
1115	1	-1.1198121489927289
1115	2	-1.1165910986178034
1115	3	-0.9127768515673615
1115	4	-0.6875167666624051
1115	5	-0.8373786208385567
1115	6	-0.8673349846270568
1115	7	-1.1277327000397648
1115	8	-0.7539062141431856
1115	9	-2.7639863993508094
1115	10	-1.7391744907708642
1115	11	-1.280247780871747
1115	12	-1.1643277553346252
1115	13	-1.504992641455992
1115	14	-1.3644579303592792
1115	15	-2.049211138768662
1115	16	-1.5579502397351916
1115	17	-3.1289821661587
1115	18	-2.8473658397593837
1115	19	-2.2002554182133807
1115	20	-1.9059251319212862
1115	21	-1.3670078232446627
1115	22	-0.9108561413733298
1115	23	-0.4119604754391375
1115	24	-1.2221333537025743
1115	25	-1.0443625221974742
1116	1	-1.7726323539442248
1116	2	-0.925969543928364
1116	3	-0.7528050828889015
1116	4	-0.4325854109417264
1116	5	-0.5400367830489046
1116	6	-0.5075286481791323
1116	7	-0.8846457698257808
1116	8	-1.82481740171783
1116	9	-0.9810215625017449
1116	10	-2.54551104964807
1116	11	-1.3244805619050766
1116	12	-0.8821772650384219
1116	13	-0.8727577134871751
1116	14	-1.301534325086088
1116	15	-1.2920844923191055
1116	16	-0.8688301752191308
1116	17	-0.7874999307998749
1116	18	-1.4798731843002528
1116	19	-0.9567188860082965
1116	20	-1.253595469110941
1116	21	-0.774079211586546
1116	22	-0.3894464584694006
1116	23	-0.23859029375177698
1116	24	-0.3324510444591766
1116	25	-0.29689216673082797
1201	1	0.2946933023608125
1201	2	0.3706746748796128
1201	3	0.3800880755659044
1201	4	0.4761703355892877
1201	5	0.48955777599633166
1201	6	0.4018491641430586
1201	7	0.33703534390485856
1201	8	0.44354080388813344
1201	9	0.6542929829083306
1201	10	0.5163956157023293
1201	11	0.39799406610055144
1201	12	0.42584866307381475
1201	13	0.4630938838048735
1201	14	1.1495782779030748
1201	15	0.8322397936088564
1201	16	0.7725885093238105
1201	17	0.7577935459808387
1201	18	0.6153044557824003
1201	19	0.606888743642962
1201	20	0.7792088654683553
1201	21	0.8279605249090878
1201	22	0.8617536203775273
1201	23	0.8138242279993761
1201	24	0.7825491561290931
1201	25	0.7960497818941974
1202	1	-0.07186593323404367
1202	2	0.12682308747426774
1202	3	0.052448580363257946
1202	4	0.19518367469915887
1202	5	0.11338231579092906
1202	6	0.05983087694615108
1202	7	0.11795143185462109
1202	8	0.1930230015060772
1202	9	0.23400999886406026
1202	10	0.40090831236854996
1202	11	0.26992686504592456
1202	12	0.19168218155086691
1202	13	0.3378852555320293
1202	14	0.5306632203963204
1202	15	0.5790787374837194
1202	16	0.5427405972635029
1202	17	0.40832020576574024
1202	18	0.43224553566203383
1202	19	0.4206983715964725
1202	20	0.5632958776095904
1202	21	0.5258359887841095
1202	22	0.4668604553169189
1202	23	0.8254965238737597
1202	24	0.4610513719112177
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2211	8	0.2846686900539055
2211	9	0.4753613494916493
2211	10	0.4635897399618969
2211	11	0.14100581197210715
2211	12	0.24377238072820973
2211	13	0.14150728139054536
2211	14	0.4901382413472746
2211	15	0.2673078947266774
2211	16	0.1831212473206918
2211	17	0.2154156282724884
2211	18	0.2619101966338562
2211	19	0.2829337718406606
2211	20	0.36483679956322135
2211	21	0.31545001429581276
2211	22	0.443015045530359
2211	23	0.5333372386601027
2211	24	0.5584694827313399
2211	25	0.6209288048624302
2212	1	-0.35702727378196997
2212	2	-0.4350300519445902
2212	3	-0.3286441305999297
2212	4	-0.6907730678859068
2212	5	-0.3511580888244481
2212	6	-0.7445892044858766
2212	7	-0.6311809253212388
2212	8	-0.5195802952855288
2212	9	-0.3905661829382936
2212	10	-0.7183572926459476
2212	11	-0.6495771429365576
2212	12	-0.7511252561529673
2212	13	-0.7015823223339979
2212	14	-0.6809082385494776
2212	15	-0.8063265726745286
2212	16	-0.576480600175671
2212	17	-0.7101687061523947
2212	18	-0.6339235513081789
2212	19	-0.7430393901612788
2212	20	-0.7091899253597376
2212	21	-0.5884552981231437
2212	22	-0.6340246408143884
2212	23	-0.14845966790669252
2212	24	-0.34260653991986345
2212	25	-1.0692748207775722
2213	1	0.02339621100275835
2213	2	-0.9745827763864163
2213	3	-0.07513104642598023
2213	4	0.16896491799894164
2213	5	0.07686633015767086
2213	6	0.14993320663312204
2213	7	0.1768584925186829
2213	8	0.23097983240013775
2213	9	0.20467100340823663
2213	10	-0.21933206811394967
2213	11	0.00617322076319482
2213	12	0.15780875789005222
2213	13	-0.23984684327228223
2213	14	0.16808174317535673
2213	15	0.07010215119019136
2213	16	0.07474853968943052
2213	17	0.23947277906516007
2213	18	0.2918040736422256
2213	19	0.2601726648325311
2213	20	0.31967476029546305
2213	21	-0.4043183185744965
2213	22	-0.7540124223059834
2213	23	0.034765883053353264
2213	24	0.22448464351706576
2213	25	-0.5362337497569314
2214	1	-0.16929004728842045
2214	2	-0.34208191303233804
2214	3	-0.33784657868257284
2214	4	-0.5330190785714635
2214	5	-0.2700796411919768
2214	6	-0.03871318420463865
2214	7	-0.3775403427095583
2214	8	-0.010033928019822724
2214	9	0.010618024470517184
2214	10	-1.6490797524566432
2214	11	-0.6874774071138816
2214	12	-0.35525591434542036
2214	13	-0.21046809822159673
2214	14	-0.7442599290473879
2214	15	-0.5235152017956324
2214	16	-0.581154865395483
2214	17	-0.3582942170334249
2214	18	-0.32830363790620964
2214	19	-1.1133981471063024
2214	20	-2.1995378279339803
2214	21	-1.6542185691832927
2214	22	-1.7810694532248021
2214	23	-2.3418046651227655
2214	24	-1.5047477385504067
2214	25	-1.6383918406678717
2215	1	-0.321029087247247
2215	2	-0.4083467418300195
2215	3	-0.8690311132819382
2215	4	-0.40897167235831944
2215	5	-0.23133547215017813
2215	6	-0.27306151010881763
2215	7	-0.39291890961254694
2215	8	-0.2545780734903218
2215	9	-0.33471115207335345
2215	10	-0.3133018078142058
2215	11	-0.6420716785213116
2215	12	-0.4976149698416279
2215	13	-0.4153448424502528
2215	14	-0.1943430438721805
2215	15	-0.679844425099283
2215	16	-0.5428340637742579
2215	17	-0.34310036493269536
2215	18	-0.44551126418017617
2215	19	-2.2671421151630824
2215	20	-2.4693558394182915
2215	21	-3.1230258311138
2215	22	-2.424782423102478
2215	23	-3.5687682272226198
2215	24	-2.4860942780122457
2215	25	-1.8661577021457205
2216	1	-1.59988051441902
2216	2	-1.2254534579579435
2216	3	-0.9154465249848833
2216	4	-1.1191445319988844
2216	5	-1.879991647138965
2216	6	-1.7468541243703726
2216	7	-1.1723241071806996
2216	8	-5.089798562345258
2216	9	-4.145277243537933
2216	10	-2.170673741292731
2216	11	-1.279222752171485
2216	12	-1.2678607917796967
2216	13	-1.988371134614944
2216	14	-3.813747231218926
2216	15	-2.1812464042110378
2216	16	-3.3123413272381868
2216	17	-2.9382476064484067
2216	18	-2.8707734641405764
2216	19	-1.8334910953774113
2216	20	-1.0683005283294875
2216	21	-0.9147274002545132
2216	22	-0.7586121458840367
2216	23	-1.0975649515343164
2216	24	-0.8339531885073299
2216	25	-0.5716061000226063
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	31.0
8	238.0
9	625.0
10	1363.0
11	2690.0
12	4438.0
13	6912.0
14	10007.0
15	14334.0
16	17763.0
17	19658.0
18	19706.0
19	20396.0
20	20767.0
21	22058.0
22	22391.0
23	23889.0
24	25263.0
25	26594.0
26	28352.0
27	31046.0
28	34808.0
29	41022.0
30	49576.0
31	60705.0
32	81431.0
33	116493.0
34	169458.0
35	273690.0
36	482676.0
37	1629351.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.922935299796364	19.65020892032042	11.351644464532674	24.075211315350547
2	13.17853780021939	22.574797152070015	37.48292651177698	26.763738535933612
3	20.695464327042256	28.265726592216815	27.723701868594464	23.31510721214646
4	12.803360126741623	18.958434397086908	34.73349871968855	33.504706756482925
5	11.510513939424456	40.335124623241164	35.23134535256828	12.923016084766095
6	31.844417293925513	34.45290340009087	18.21203600240671	15.49064330357691
7	24.554581391536786	33.71546129473295	22.906190276518377	18.82376703721189
8	25.932663211073777	36.68609337713501	21.548423219472305	15.83282019231891
9	26.037927788079813	17.677072624786007	20.892453211865856	35.392546375268324
10	15.557144940103907	32.223683705653485	32.827233729059394	19.391937625183218
11	29.594928937594005	27.581453172483656	20.407864444239802	22.415753445682537
12	22.286592908971805	30.989405389210894	29.347906832256193	17.376094869561108
13	27.965307474996525	23.082151012678782	23.675493385256292	25.2770481270684
14	20.656178122504958	26.329452594504183	26.58374141614071	26.43062786685015
15	28.860860599061724	26.801745178519187	22.031297915897326	22.306096306521763
16	19.27538976824027	30.040418818402642	30.311197581317277	20.37299383203981
17	24.12003781878906	30.328427941700127	27.563573629989808	17.987960609521004
18	24.203319945334727	27.22688544236264	28.87436441796587	19.695430194336765
19	22.226120653057766	31.18732389913071	29.300943819314178	17.285611628497346
20	22.791468509101	28.484623750544475	27.97650748882884	20.747400251525683
21	19.457985821389816	30.095660765261545	30.16263374627194	20.283719667076696
22	21.26255971692847	29.554100288496777	24.25455793267835	24.9287820618964
23	18.225964168941807	30.699524962144125	27.886193672777164	23.188317196136904
24	20.475170035764528	34.07612801313321	25.703249872532652	19.745452078569613
25	21.552875172899245	27.825118260621306	23.729067500849112	26.89293906563034
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7011.0
1	7011.0
2	8894.0
3	10777.0
4	10777.0
5	10777.0
6	13475.0
7	16173.0
8	16173.0
9	16173.0
10	18186.5
11	20200.0
12	20200.0
13	20200.0
14	26096.0
15	31992.0
16	31992.0
17	31992.0
18	44389.5
19	56787.0
20	56787.0
21	56787.0
22	76669.0
23	96551.0
24	96551.0
25	96551.0
26	121600.5
27	146650.0
28	146650.0
29	146650.0
30	172300.0
31	197950.0
32	197950.0
33	197950.0
34	220645.0
35	243340.0
36	243340.0
37	243340.0
38	259821.0
39	276302.0
40	276302.0
41	276302.0
42	307058.5
43	337815.0
44	337815.0
45	337815.0
46	526708.0
47	715601.0
48	715601.0
49	715601.0
50	617308.0
51	519015.0
52	519015.0
53	519015.0
54	388049.5
55	257084.0
56	257084.0
57	257084.0
58	202207.0
59	147330.0
60	147330.0
61	147330.0
62	119965.5
63	92601.0
64	92601.0
65	92601.0
66	70677.5
67	48754.0
68	48754.0
69	48754.0
70	35609.0
71	22464.0
72	22464.0
73	22464.0
74	15699.5
75	8935.0
76	8935.0
77	8935.0
78	5994.5
79	3054.0
80	3054.0
81	3054.0
82	2031.5
83	1009.0
84	1009.0
85	1009.0
86	630.5
87	252.0
88	252.0
89	252.0
90	154.0
91	56.0
92	56.0
93	56.0
94	37.0
95	18.0
96	18.0
97	18.0
98	14.5
99	11.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03032784771736902
2	0.015010442847969077
3	0.021026898468013942
4	0.021855695925877265
5	0.020719936446583084
6	0.0052797467686108
7	3.069620214308605E-4
8	5.525316385755489E-4
9	4.604430321462907E-4
10	2.1487341500160237E-4
11	9.822784685787535E-4
12	0.0011664556814372697
13	0.002609177182162314
14	5.832278407186349E-4
15	0.0021794303521591096
16	0.0027933543950208303
17	0.0028240505971639162
18	0.0027012657885915723
19	3.3765822357394653E-4
20	3.9905062786011863E-4
21	0.0825113913606153
22	0.07928829013559126
23	0.0805161382213147
24	0.0010743670750080119
25	0.22359113641023878
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3257732.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.354710998278094
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.10553803057412	56.80859494239085
2	6.754796398693176	8.423867545854453
3	1.064496027710473	1.9912902650010469
4	0.3485344079922666	0.8693104913325491
5	0.18857514825690003	0.5879274435508225
6	0.10715869105188923	0.40091095268965926
7	0.06839516095822437	0.29853323466615567
8	0.045917856928904856	0.2290555758769715
9	0.039854557880909566	0.22366074947654369
>10	0.23388704619800738	2.7006917034970885
>50	0.020970274562510466	0.8977465265389242
>100	0.015343655806065068	1.9426788630938367
>500	0.0022939404258086223	1.0058369427978897
>1k	0.002493413506287931	3.0850496369066565
>5k	9.973654025151725E-4	4.803433447846106
>10k+	7.480240518863794E-4	15.731411678480475
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	103402	3.1740486939993837	No Hit
CCCATGTACTCTGCGTTGATACCAC	97263	2.9856047090429785	No Hit
GAGTACATGGGAAGCAGTGGTATCA	48998	1.5040525126069302	No Hit
CATGTACTCTGCGTTGATACCACTG	38259	1.1744059977923291	No Hit
GCGTTGATACCACTGCTTCCCATGT	30722	0.9430487222398896	No Hit
GCTTCCCATGTACTCTGCGTTGATA	30384	0.9326734059155265	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	26469	0.8124977745253446	No Hit
GTATCAACGCAGAGTACATGGGAAG	25103	0.770566762397889	No Hit
ACTCTGCGTTGATACCACTGCTTCC	20529	0.6301623337954135	No Hit
TATCAACGCAGAGTACATGGGAAGC	19679	0.6040705619737904	No Hit
GGTATCAACGCAGAGTACATGGGAA	16859	0.5175072719302877	No Hit
CAGTGGTATCAACGCAGAGTACATG	13744	0.42188860225457464	No Hit
GTGGTATCAACGCAGAGTACATGGG	12931	0.3969325899122457	No Hit
GCAGTGGTATCAACGCAGAGTACAT	11407	0.35015157784618256	No Hit
ATACCACTGCTTCCCATGTACTCTG	10162	0.3119348061780404	No Hit
CATGGGAAGCAGTGGTATCAACGCA	9792	0.3005772113850986	No Hit
GTACATGGTAAGCAGTGGTATCAAC	9775	0.30005537594866616	No Hit
ACATGGGAAGCAGTGGTATCAACGC	9693	0.2975382873729331	No Hit
GTACTCTGCGTTGATACCACTGCTT	9652	0.29627974308506655	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	9258	0.28418543944069063	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	9107	0.27955031291708465	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	8027	0.24639841460255169	No Hit
CTTCCCATGTACTCTGCGTTGATAC	7935	0.24357436400538782	No Hit
TACCACTGCTTCCCATGTACTCTGC	7902	0.24256138933466598	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	7892	0.2422544273132351	No Hit
GTTGATACCACTGCTTCCCATGTAC	7826	0.24022847797179142	No Hit
GATACCACTGCTTCCCATGTACTCT	7636	0.23439619956460508	No Hit
CCACTGCTTCCCATGTACTCTGCGT	7619	0.23387436412817258	No Hit
ACCATGTACTCTGCGTTGATACCAC	6729	0.206554744220826	No Hit
GTACATGGGTAAGCAGTGGTATCAA	6648	0.20406835184723607	No Hit
CCATGTACTCTGCGTTGATACCACT	6615	0.20305537717651423	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	6432	0.19743797218432949	No Hit
TTCCCATGTACTCTGCGTTGATACC	5680	0.17435442817272875	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	5165	0.15854588406903944	No Hit
TGATACCACTGCTTCCCATGTACTC	5092	0.15630506131259417	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	4606	0.14138670707105433	No Hit
CCCCATGTACTCTGCGTTGATACCA	4587	0.1408034792303357	No Hit
GCTTACCATGTACTCTGCGTTGATA	3931	0.12066677062447125	No Hit
ATCAACGCAGAGTACATGGGAAGCA	3724	0.11431265678085245	No Hit
GAGTACATGGTAAGCAGTGGTATCA	3443	0.10568702397864528	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	3.069620214308605E-5	0.0	0.0	0.0	0.0
9	9.208860642925815E-5	0.0	0.0	3.069620214308605E-5	0.0
10	9.208860642925815E-5	0.0	0.0	3.069620214308605E-5	0.0
11	9.208860642925815E-5	0.0	0.0	6.13924042861721E-5	0.0
12	9.208860642925815E-5	0.0	0.0	9.208860642925815E-5	0.0
13	9.208860642925815E-5	0.0	0.0	9.208860642925815E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTACA	40	0.005284825	14.248342	4
ACCGATG	55	0.00307979	12.085605	8
CGCAAAA	215	0.0	11.929944	2
TAACCCG	60	0.005885111	11.081534	5
CCATCGA	75	0.0026585623	10.128888	9
AGGACCG	85	6.606934E-4	10.05719	5
CTTAGAC	265	0.0	10.036722	3
GTCTAGA	280	0.0	9.841162	1
GCTAGAC	155	4.0745363E-8	9.805462	3
TTATACT	490	0.0	9.69275	4
ACGCAAA	295	0.0	9.66286	1
CGTGCAC	90	0.0011185259	9.495979	10
CGTTCCA	160	6.686059E-8	9.495978	10
GTACAAA	665	0.0	9.43037	1
CTACACT	385	0.0	9.375532	4
TTAGACA	335	0.0	9.3571205	4
TCAGAAC	255	0.0	9.312784	3
TATACTG	690	0.0	9.22314	5
GTCCTAC	165	1.0636177E-7	9.213879	1
CCAGGAC	460	0.0	9.086039	3
>>END_MODULE