FastQCFastQC Report
Wed 25 May 2016
SRR1294778_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294778_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3257732
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1076323.3038936290646372No Hit
GTACATGGGAAGCAGTGGTATCAAC1018253.125640783219737No Hit
GAGTACATGGGAAGCAGTGGTATCA520011.5962332076426178No Hit
CATGTACTCTGCGTTGATACCACTG442761.359105046087278No Hit
GCTTCCCATGTACTCTGCGTTGATA339071.0408161260656186No Hit
GCGTTGATACCACTGCTTCCCATGT332051.0192673921611721No Hit
ACGCAGAGTACATGGGAAGCAGTGG318180.9766917597887118No Hit
GTATCAACGCAGAGTACATGGGAAG260290.7989914455823868No Hit
ACTCTGCGTTGATACCACTGCTTCC234520.719887332659654No Hit
TATCAACGCAGAGTACATGGGAAGC227320.697786067116632No Hit
GGTATCAACGCAGAGTACATGGGAA181690.5577192967377304No Hit
CAGTGGTATCAACGCAGAGTACATG166090.5098332213945161No Hit
GTGGTATCAACGCAGAGTACATGGG139330.42769018445961793No Hit
GCAGTGGTATCAACGCAGAGTACAT129230.396687020295101No Hit
CATGGGAAGCAGTGGTATCAACGCA127250.39060917227076997No Hit
ACATGGGAAGCAGTGGTATCAACGC121680.37351138767707104No Hit
ATACCACTGCTTCCCATGTACTCTG112950.3467136032061569No Hit
GGGAAGCAGTGGTATCAACGCAGAG108140.33194872997533253No Hit
CTGCTTCCCATGTACTCTGCGTTGA103060.3163550592866448No Hit
AAGCAGTGGTATCAACGCAGAGTAC98450.30220411009868214No Hit
GTACTCTGCGTTGATACCACTGCTT94930.2913990469443159No Hit
GTACATGGTAAGCAGTGGTATCAAC94270.2893730976028722No Hit
CTTCCCATGTACTCTGCGTTGATAC93940.28836012293215035No Hit
GCAGAGTACATGGGAAGCAGTGGTA88970.2731041104670366No Hit
TACCACTGCTTCCCATGTACTCTGC88360.2712316421363083No Hit
GATACCACTGCTTCCCATGTACTCT86800.26644303460198693No Hit
CCACTGCTTCCCATGTACTCTGCGT85810.2634041105898214No Hit
GTATCAACGCAGAGTACTTTTTTTT85610.2627901865469597No Hit
GTTGATACCACTGCTTCCCATGTAC81730.2508800601154423No Hit
CCATGTACTCTGCGTTGATACCACT81220.24931455380614487No Hit
ATGGGAAGCAGTGGTATCAACGCAG78130.23982942734393128No Hit
ACCATGTACTCTGCGTTGATACCAC75220.23089683252029325No Hit
GTACATGGGTAAGCAGTGGTATCAA70230.21557942765089333No Hit
GGTATCAACGCAGAGTACTTTTTTT68970.2117117061808645No Hit
TATCAACGCAGAGTACTTTTTTTTT66060.20277911135722645No Hit
TTCCCATGTACTCTGCGTTGATACC59200.18172151668706943No Hit
GGTAAGCAGTGGTATCAACGCAGAG55580.17060949151127225No Hit
CCCCATGTACTCTGCGTTGATACCA54630.1676933523076791No Hit
TGATACCACTGCTTCCCATGTACTC52440.16097088403834323No Hit
ACGCAGAGTACTTTTTTTTTTTTTT47120.14464050449822147No Hit
ATCAACGCAGAGTACATGGGAAGCA45930.14098765644319422No Hit
GCTTACCATGTACTCTGCGTTGATA44700.13721202357959464No Hit
GTACTTTTTTTTTTTTTTTTTTTTT44260.13586139068529887No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA40270.12361360603020753No Hit
GGAAGCAGTGGTATCAACGCAGAGT38180.11719809978230254No Hit
GAGTACATGGTAAGCAGTGGTATCA36390.11170347959869012No Hit
AAAAAGTACTCTGCGTTGATACCAC35900.11019936569367891No Hit
GAGTACTTTTTTTTTTTTTTTTTTT32590.10003892278431745No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACCG600.00580514911.1019185
AGGACCG600.00580514911.1019185
AGTACTC10600.010.413665
GTACGGG750.002600691510.1557941
GTCTAGG1803.783498E-1010.0500041
CGCAAAA1252.5177287E-69.8965682
CGTGGGA1502.5871486E-79.515934
ACGTACT1606.473056E-89.515934
TGCGTTA900.00112110859.49358512
AAAAGTA9100.09.4113592
CTACACT4050.09.398454
AAGTACT11750.09.3134644
GTCTAGT1554.1298335E-79.2139261
GGTCTAA1554.1298335E-79.2139261
TCTGGAC2600.09.1499343
AAAAAGT9200.09.1070991
CGCAGAA1054.4201541E-49.0627922
CCCCTAT950.00178818159.0199491
TAGACTG2750.08.996885
GGTACTC1701.6438025E-78.956175
Produced by FastQC (version 0.11.5)