FastQCFastQC Report
Wed 25 May 2016
SRR1294777_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294777_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2796646
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC769392.751116873569268No Hit
CCCATGTACTCTGCGTTGATACCAC726782.5987557953348404No Hit
GAGTACATGGGAAGCAGTGGTATCA358761.2828223522033178No Hit
CATGTACTCTGCGTTGATACCACTG283541.0138573133675124No Hit
GCTTCCCATGTACTCTGCGTTGATA233170.8337487118498372No Hit
GCGTTGATACCACTGCTTCCCATGT225170.8051430177433969No Hit
ACGCAGAGTACATGGGAAGCAGTGG193870.6932232395519491No Hit
GTATCAACGCAGAGTACATGGGAAG181340.6484195711577369No Hit
ACTCTGCGTTGATACCACTGCTTCC152670.5459039149037811No Hit
TATCAACGCAGAGTACATGGGAAGC146550.5240205589123543No Hit
GGTATCAACGCAGAGTACATGGGAA123940.44317371594402727No Hit
CAGTGGTATCAACGCAGAGTACATG98230.35124216650945456No Hit
GTGGTATCAACGCAGAGTACATGGG95670.3420883443953936No Hit
GCAGTGGTATCAACGCAGAGTACAT82420.2947101635316018No Hit
GTACATGGTAAGCAGTGGTATCAAC76590.2738637639515334No Hit
ATACCACTGCTTCCCATGTACTCTG75040.26832141071841054No Hit
ACATGGGAAGCAGTGGTATCAACGC73040.26116998719180046No Hit
GTACTCTGCGTTGATACCACTGCTT71410.25534157701761323No Hit
CATGGGAAGCAGTGGTATCAACGCA71050.2540543207828234No Hit
CTGCTTCCCATGTACTCTGCGTTGA70970.25376826384175905No Hit
GGGAAGCAGTGGTATCAACGCAGAG69010.24675986878568115No Hit
CTTCCCATGTACTCTGCGTTGATAC59840.2139705919161739No Hit
TACCACTGCTTCCCATGTACTCTGC59500.21275484991665014No Hit
AAGCAGTGGTATCAACGCAGAGTAC58280.208392481565418No Hit
GCAGAGTACATGGGAAGCAGTGGTA58060.20760582497749092No Hit
CCACTGCTTCCCATGTACTCTGCGT55980.20016834450981638No Hit
GATACCACTGCTTCCCATGTACTCT55780.1994532021571554No Hit
ACCATGTACTCTGCGTTGATACCAC55700.19916714521609097No Hit
GTTGATACCACTGCTTCCCATGTAC55040.19680717545230964No Hit
CCATGTACTCTGCGTTGATACCACT50940.18214675722275897No Hit
GTACATGGGTAAGCAGTGGTATCAA49240.17606804722514038No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA48800.17449473404928617No Hit
ATGGGAAGCAGTGGTATCAACGCAG47170.16866632387509897No Hit
TTCCCATGTACTCTGCGTTGATACC43370.1550786191745398No Hit
TGATACCACTGCTTCCCATGTACTC39390.14084728635658572No Hit
CCCCATGTACTCTGCGTTGATACCA35030.12525718306857572No Hit
GGTAAGCAGTGGTATCAACGCAGAG34590.1236838698927215No Hit
GCTTACCATGTACTCTGCGTTGATA33430.11953604424728764No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTTCG350.002176523216.2792789
CGCAAAA2050.012.9755862
AAGCGGT1251.4206307E-912.15519410
GCACCGC600.00589261711.0795476
CGTTCCG600.0058949711.07895310
GTCTAGG2150.010.6055791
ACCGCGC856.5684185E-410.06348518
GTATTAG3150.09.9532531
CCGCGCC800.0044360839.52092919
GTCCAAG2303.6379788E-129.5008321
TATTCCG1106.8521374E-59.4984535
GGACAGT3350.09.3550116
GTACAAA5350.09.2344531
AGCGGTG1554.2877764E-79.18991511
TCAGTAC2002.6611815E-99.0243373
CACCGCG950.00181673229.0041717
CAGCTCG850.0074620178.9376429
ACGCAAA3750.08.8674431
TGGACAG3550.08.8295485
GGAAGCG2054.1727617E-98.8013988
Produced by FastQC (version 0.11.5)