##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294777_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2796646 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.836530973172867 33.0 33.0 33.0 27.0 33.0 2 30.81690031559232 33.0 33.0 33.0 27.0 33.0 3 30.800681602176322 33.0 33.0 33.0 27.0 33.0 4 34.14731396108052 37.0 37.0 37.0 27.0 37.0 5 34.49423845563579 37.0 37.0 37.0 27.0 37.0 6 34.65727660919544 37.0 37.0 37.0 27.0 37.0 7 34.66611648381669 37.0 37.0 37.0 27.0 37.0 8 34.55996111055886 37.0 37.0 37.0 27.0 37.0 9 34.286303307604896 37.0 37.0 37.0 27.0 37.0 10 34.392516964964464 37.0 37.0 37.0 27.0 37.0 11 34.65919319070058 37.0 37.0 37.0 27.0 37.0 12 34.591284345605416 37.0 37.0 37.0 27.0 37.0 13 34.46485611693436 37.0 37.0 37.0 27.0 37.0 14 35.640387092252645 40.0 37.0 40.0 27.0 40.0 15 35.89010014138364 40.0 37.0 40.0 27.0 40.0 16 35.87586666313863 37.0 37.0 40.0 27.0 40.0 17 35.857074867537754 37.0 37.0 40.0 27.0 40.0 18 35.84812521856538 37.0 37.0 40.0 27.0 40.0 19 35.858080715256776 40.0 37.0 40.0 27.0 40.0 20 35.795754271366484 40.0 37.0 40.0 27.0 40.0 21 35.71857682380966 37.0 37.0 40.0 27.0 40.0 22 35.624799134391694 37.0 37.0 40.0 27.0 40.0 23 35.276061038830086 37.0 33.0 40.0 27.0 40.0 24 35.614217888141724 37.0 37.0 40.0 27.0 40.0 25 34.485482252669804 37.0 33.0 40.0 22.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4255174061726166 1101 2 0.5514752343062348 1101 3 0.3790033574149412 1101 4 0.48472941070858155 1101 5 0.4435231219310438 1101 6 0.2766521984892236 1101 7 0.33316284111321437 1101 8 0.25892398627368607 1101 9 0.5307464543943254 1101 10 0.6065305542515063 1101 11 0.3373653307827027 1101 12 0.4563158073628415 1101 13 0.5383174820361774 1101 14 1.0270795986529322 1101 15 0.7533622662830552 1101 16 0.6342629811747784 1101 17 0.6289529559816529 1101 18 0.5396087709783188 1101 19 0.6563370294554502 1101 20 0.8209929920977359 1101 21 0.6934962778011098 1101 22 0.7814078434459759 1101 23 1.1097978108254267 1101 24 0.843346456663383 1101 25 0.7997194812139696 1102 1 0.3010790448502121 1102 2 0.5733568000231308 1102 3 0.34683430589647557 1102 4 0.3381940159715029 1102 5 0.23480191958368835 1102 6 0.31258453937591923 1102 7 0.3176740478150535 1102 8 0.46806484298528517 1102 9 0.5706425736443492 1102 10 0.6823840206742702 1102 11 0.35976619997362747 1102 12 0.46786200556755375 1102 13 0.4970859393701872 1102 14 1.1568293828718694 1102 15 0.8175728309939387 1102 16 0.5833786987348546 1102 17 0.7058781830500109 1102 18 0.7358047069491889 1102 19 0.7757379715970458 1102 20 0.8983598088689675 1102 21 0.8133219507710265 1102 22 0.8679347949578471 1102 23 1.1887166468135177 1102 24 0.9651392281294946 1102 25 0.8569577418594392 1103 1 0.21688481413420035 1103 2 0.3600987060793557 1103 3 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16 -0.06715436111885964 2213 17 9.477249383422759E-4 2213 18 0.016599603829781984 2213 19 0.053421137694869 2213 20 0.2767287507592968 2213 21 -0.35823453552058027 2213 22 -0.7626431270634626 2213 23 -0.0700830722503909 2213 24 0.0683745223096821 2213 25 -0.4150289304747119 2214 1 -0.052191826955237275 2214 2 -0.24262395636857548 2214 3 -0.20161483175531814 2214 4 -0.3149688474890695 2214 5 -0.17583231958241186 2214 6 -0.06832287653745084 2214 7 -0.2787675615405334 2214 8 0.05349637121483397 2214 9 0.06690654728906509 2214 10 -1.8521474553738102 2214 11 -0.7417531035235712 2214 12 -0.3392211858551022 2214 13 -0.15775803179564463 2214 14 -0.5908276067887002 2214 15 -0.26969692219085317 2214 16 -0.46872949500524186 2214 17 -0.21204286982824527 2214 18 -0.15944907099940053 2214 19 -0.9675492108224901 2214 20 -2.0607649173345663 2214 21 -1.6803823709167744 2214 22 -1.7405117804901948 2214 23 -2.2510912503983675 2214 24 -1.340479601360009 2214 25 -1.7186873137643346 2215 1 -0.04287862302521006 2215 2 -0.242551746128143 2215 3 -0.7192906844592066 2215 4 -0.17748949610317766 2215 5 -0.06138803489513833 2215 6 -0.0703869391622618 2215 7 -0.08027503018819004 2215 8 -0.15033780084363713 2215 9 -0.02815535861396512 2215 10 -0.11487642733643355 2215 11 -0.36448079742883266 2215 12 -0.25759997010304403 2215 13 -0.10131901909495156 2215 14 0.04476117825802817 2215 15 -0.45564276451052876 2215 16 -0.14944073392209134 2215 17 0.008711503524253317 2215 18 -0.07680604839376315 2215 19 -1.9338391700844113 2215 20 -2.1476376707493756 2215 21 -3.0085127327667607 2215 22 -2.16280876906756 2215 23 -3.345611259983798 2215 24 -2.3076463079659675 2215 25 -1.520448476796524 2216 1 -1.4855891317009942 2216 2 -1.158153135891176 2216 3 -0.7923261909602886 2216 4 -0.753439145336408 2216 5 -1.6755079863864708 2216 6 -1.480975746350552 2216 7 -1.087097190665908 2216 8 -5.000463421913182 2216 9 -3.881512434585286 2216 10 -1.7467178428888843 2216 11 -0.8859435895425918 2216 12 -1.0893406841219502 2216 13 -1.842480054560518 2216 14 -3.3705619109457245 2216 15 -1.7518869452615888 2216 16 -2.763971924277662 2216 17 -2.5729195952379555 2216 18 -2.4787153249085065 2216 19 -1.5336705973546714 2216 20 -0.7979262154382383 2216 21 -0.6147552863284176 2216 22 -0.5655392952433047 2216 23 -0.9267310733175407 2216 24 -0.4350974027369645 2216 25 -0.08442532772448175 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 3.0 7 98.0 8 491.0 9 1149.0 10 2042.0 11 3376.0 12 5244.0 13 7679.0 14 10257.0 15 13973.0 16 16935.0 17 18231.0 18 18682.0 19 18960.0 20 19311.0 21 20975.0 22 21494.0 23 22383.0 24 23390.0 25 25044.0 26 26447.0 27 29466.0 28 32903.0 29 37994.0 30 45804.0 31 55721.0 32 74550.0 33 105182.0 34 152808.0 35 241021.0 36 419297.0 37 1325736.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.53681693221919 19.97341732580873 11.582098328710934 23.907667413261148 2 13.350608869674247 22.66752543677293 37.820979334086005 26.16088635946681 3 20.554019691643035 28.102363640915414 27.860098493979134 23.483518173462418 4 12.682165485729326 18.669575019920718 35.18306316977601 33.465196324573945 5 11.938243987747505 39.848578320664195 34.85255698429814 13.360620707290163 6 31.84429162135555 34.952565917622145 18.098867582580073 15.104274878442231 7 24.59477309045166 33.46093870401049 23.00211360549058 18.94217460004727 8 25.72273567732032 36.876942292351046 21.373931705684445 16.026390324644186 9 25.773941251371742 17.512417637389614 21.154662384365167 35.55897872687348 10 15.660757194347502 31.941544138680705 32.73231449167572 19.66538417529607 11 30.117091429719757 27.368950639665478 20.585685996442844 21.928271934171917 12 22.000848180666775 30.61982268304003 29.756945462555258 17.622383673737936 13 27.720463581676576 23.28682283637867 24.107327074959244 24.885386506985512 14 20.57912662584365 25.892388146426498 26.87003084074554 26.65845438698431 15 28.208982709330456 27.335098575231665 22.09443984559795 22.361478869839928 16 19.81018965658424 30.21067510649611 29.560238019209883 20.418897217709766 17 23.70052549674747 30.312716334738855 27.699249658155356 18.28750851035832 18 23.70054058770229 27.7375342649931 28.844383830856625 19.717541316447985 19 22.13731859895911 30.758894171030544 29.238102219274236 17.86568501073612 20 22.77748575964643 28.462041814612586 27.943310341375156 20.817162084365826 21 20.028558749427393 30.077838696747595 29.622222858451675 20.27137969537334 22 21.177354298844783 29.541645746603795 24.97664050570308 24.304359448848345 23 18.73192545908126 30.624789976563267 27.851102822106615 22.79218174224886 24 20.596614120999853 33.18272268438809 26.18801658433499 20.032646610277066 25 21.751087665479254 27.892079215017308 24.057404367863874 26.299428751639564 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6485.0 1 6485.0 2 7636.5 3 8788.0 4 8788.0 5 8788.0 6 10742.0 7 12696.0 8 12696.0 9 12696.0 10 14547.0 11 16398.0 12 16398.0 13 16398.0 14 21512.5 15 26627.0 16 26627.0 17 26627.0 18 37450.5 19 48274.0 20 48274.0 21 48274.0 22 65851.5 23 83429.0 24 83429.0 25 83429.0 26 106237.5 27 129046.0 28 129046.0 29 129046.0 30 153340.0 31 177634.0 32 177634.0 33 177634.0 34 198228.5 35 218823.0 36 218823.0 37 218823.0 38 236652.5 39 254482.0 40 254482.0 41 254482.0 42 277098.5 43 299715.0 44 299715.0 45 299715.0 46 435842.5 47 571970.0 48 571970.0 49 571970.0 50 497391.5 51 422813.0 52 422813.0 53 422813.0 54 322154.0 55 221495.0 56 221495.0 57 221495.0 58 176898.5 59 132302.0 60 132302.0 61 132302.0 62 108265.5 63 84229.0 64 84229.0 65 84229.0 66 64810.0 67 45391.0 68 45391.0 69 45391.0 70 33402.0 71 21413.0 72 21413.0 73 21413.0 74 15227.0 75 9041.0 76 9041.0 77 9041.0 78 6266.0 79 3491.0 80 3491.0 81 3491.0 82 2430.5 83 1370.0 84 1370.0 85 1370.0 86 939.0 87 508.0 88 508.0 89 508.0 90 341.0 91 174.0 92 174.0 93 174.0 94 108.0 95 42.0 96 42.0 97 42.0 98 26.0 99 10.0 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.030035978811762377 2 0.014267089935587129 3 0.01916581505131504 4 0.02009550010977435 5 0.017199173581497265 6 0.008545951114299058 7 1.0727135289915133E-4 8 5.363567644957567E-4 9 6.793852350279585E-4 10 2.1454270579830266E-4 11 0.0010011992937254124 12 0.0025745124695796323 13 0.0026102695872126827 14 7.508994702940594E-4 15 0.001823612999285573 16 0.002324212646148279 17 0.0019308843521847242 18 0.002145427057983027 19 3.933282939635549E-4 20 5.721138821288071E-4 21 0.08603162502511938 22 0.08149047108572197 23 0.08277772732051178 24 0.0011084706466245639 25 0.22405409908869411 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2796646.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.7140862318723 #Duplication Level Percentage of deduplicated Percentage of total 1 92.7686507725188 61.88975767252265 2 5.458443262088639 7.2831010895752755 3 0.8354159121929512 1.6720202761655638 4 0.2908406195826283 0.7761266469826653 5 0.15068551789988605 0.5026423317533667 6 0.09901320913394049 0.3963345463353667 7 0.06927944475555783 0.3235340396062963 8 0.04561891460637763 0.24347393622834368 9 0.033948917307733306 0.20383838970721396 >10 0.2061200346122602 2.5452460778514965 >50 0.021647457300115337 1.011797908728768 >100 0.014267851523367986 1.9394173023019565 >500 0.002167173742047997 1.0243564976967494 >1k 0.0022755324291472948 3.244434142190055 >5k 0.0010294075274713953 4.671954802740339 >10k+ 5.959727790623868E-4 12.271964339613897 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 76939 2.751116873569268 No Hit CCCATGTACTCTGCGTTGATACCAC 72678 2.5987557953348404 No Hit GAGTACATGGGAAGCAGTGGTATCA 35876 1.2828223522033178 No Hit CATGTACTCTGCGTTGATACCACTG 28354 1.0138573133675124 No Hit GCTTCCCATGTACTCTGCGTTGATA 23317 0.8337487118498372 No Hit GCGTTGATACCACTGCTTCCCATGT 22517 0.8051430177433969 No Hit ACGCAGAGTACATGGGAAGCAGTGG 19387 0.6932232395519491 No Hit GTATCAACGCAGAGTACATGGGAAG 18134 0.6484195711577369 No Hit ACTCTGCGTTGATACCACTGCTTCC 15267 0.5459039149037811 No Hit TATCAACGCAGAGTACATGGGAAGC 14655 0.5240205589123543 No Hit GGTATCAACGCAGAGTACATGGGAA 12394 0.44317371594402727 No Hit CAGTGGTATCAACGCAGAGTACATG 9823 0.35124216650945456 No Hit GTGGTATCAACGCAGAGTACATGGG 9567 0.3420883443953936 No Hit GCAGTGGTATCAACGCAGAGTACAT 8242 0.2947101635316018 No Hit GTACATGGTAAGCAGTGGTATCAAC 7659 0.2738637639515334 No Hit ATACCACTGCTTCCCATGTACTCTG 7504 0.26832141071841054 No Hit ACATGGGAAGCAGTGGTATCAACGC 7304 0.26116998719180046 No Hit GTACTCTGCGTTGATACCACTGCTT 7141 0.25534157701761323 No Hit CATGGGAAGCAGTGGTATCAACGCA 7105 0.2540543207828234 No Hit CTGCTTCCCATGTACTCTGCGTTGA 7097 0.25376826384175905 No Hit GGGAAGCAGTGGTATCAACGCAGAG 6901 0.24675986878568115 No Hit CTTCCCATGTACTCTGCGTTGATAC 5984 0.2139705919161739 No Hit TACCACTGCTTCCCATGTACTCTGC 5950 0.21275484991665014 No Hit AAGCAGTGGTATCAACGCAGAGTAC 5828 0.208392481565418 No Hit GCAGAGTACATGGGAAGCAGTGGTA 5806 0.20760582497749092 No Hit CCACTGCTTCCCATGTACTCTGCGT 5598 0.20016834450981638 No Hit GATACCACTGCTTCCCATGTACTCT 5578 0.1994532021571554 No Hit ACCATGTACTCTGCGTTGATACCAC 5570 0.19916714521609097 No Hit GTTGATACCACTGCTTCCCATGTAC 5504 0.19680717545230964 No Hit CCATGTACTCTGCGTTGATACCACT 5094 0.18214675722275897 No Hit GTACATGGGTAAGCAGTGGTATCAA 4924 0.17606804722514038 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 4880 0.17449473404928617 No Hit ATGGGAAGCAGTGGTATCAACGCAG 4717 0.16866632387509897 No Hit TTCCCATGTACTCTGCGTTGATACC 4337 0.1550786191745398 No Hit TGATACCACTGCTTCCCATGTACTC 3939 0.14084728635658572 No Hit CCCCATGTACTCTGCGTTGATACCA 3503 0.12525718306857572 No Hit GGTAAGCAGTGGTATCAACGCAGAG 3459 0.1236838698927215 No Hit GCTTACCATGTACTCTGCGTTGATA 3343 0.11953604424728764 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.5757117633050446E-5 0.0 10 3.5757117633050446E-5 0.0 0.0 7.151423526610089E-5 0.0 11 3.5757117633050446E-5 0.0 0.0 7.151423526610089E-5 0.0 12 3.5757117633050446E-5 0.0 0.0 7.151423526610089E-5 0.0 13 3.5757117633050446E-5 0.0 0.0 7.151423526610089E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGTTCG 35 0.0021765232 16.279278 9 CGCAAAA 205 0.0 12.975586 2 AAGCGGT 125 1.4206307E-9 12.155194 10 GCACCGC 60 0.005892617 11.079547 6 CGTTCCG 60 0.00589497 11.078953 10 GTCTAGG 215 0.0 10.605579 1 ACCGCGC 85 6.5684185E-4 10.063485 18 GTATTAG 315 0.0 9.953253 1 CCGCGCC 80 0.004436083 9.520929 19 GTCCAAG 230 3.6379788E-12 9.500832 1 TATTCCG 110 6.8521374E-5 9.498453 5 GGACAGT 335 0.0 9.355011 6 GTACAAA 535 0.0 9.234453 1 AGCGGTG 155 4.2877764E-7 9.189915 11 TCAGTAC 200 2.6611815E-9 9.024337 3 CACCGCG 95 0.0018167322 9.00417 17 CAGCTCG 85 0.007462017 8.937642 9 ACGCAAA 375 0.0 8.867443 1 TGGACAG 355 0.0 8.829548 5 GGAAGCG 205 4.1727617E-9 8.801398 8 >>END_MODULE