FastQCFastQC Report
Wed 25 May 2016
SRR1294777_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294777_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2796646
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC806252.8829176091646924No Hit
GTACATGGGAAGCAGTGGTATCAAC759182.7146088564659236No Hit
GAGTACATGGGAAGCAGTGGTATCA388091.3876979782210548No Hit
CATGTACTCTGCGTTGATACCACTG325491.163858421838159No Hit
GCTTCCCATGTACTCTGCGTTGATA258550.9245002764025192No Hit
GCGTTGATACCACTGCTTCCCATGT243780.8716870136585039No Hit
ACGCAGAGTACATGGGAAGCAGTGG240770.8609241212509557No Hit
GTATCAACGCAGAGTACATGGGAAG193000.6901123703178736No Hit
ACTCTGCGTTGATACCACTGCTTCC176230.630147684047248No Hit
TATCAACGCAGAGTACATGGGAAGC170000.6078709997618577No Hit
GGTATCAACGCAGAGTACATGGGAA142250.5086449983301427No Hit
CAGTGGTATCAACGCAGAGTACATG123520.44167191700343916No Hit
GTGGTATCAACGCAGAGTACATGGG103430.3698358676786408No Hit
GCAGTGGTATCAACGCAGAGTACAT96640.3455567848057995No Hit
CATGGGAAGCAGTGGTATCAACGCA95280.34069381680770466No Hit
ACATGGGAAGCAGTGGTATCAACGC91510.32721338346004464No Hit
ATACCACTGCTTCCCATGTACTCTG84250.30125371605845No Hit
GGGAAGCAGTGGTATCAACGCAGAG81490.2913847515917281No Hit
CTGCTTCCCATGTACTCTGCGTTGA76070.2720043938346147No Hit
GTACATGGTAAGCAGTGGTATCAAC75260.26910806730633763No Hit
GTATCAACGCAGAGTACTTTTTTTT71640.25616399072317336No Hit
AAGCAGTGGTATCAACGCAGAGTAC71130.25434037772388784No Hit
GTACTCTGCGTTGATACCACTGCTT70450.2519088937248404No Hit
CTTCCCATGTACTCTGCGTTGATAC68520.24500777002166166No Hit
GCAGAGTACATGGGAAGCAGTGGTA66080.23628303331919734No Hit
TACCACTGCTTCCCATGTACTCTGC65090.23274307867352534No Hit
CCACTGCTTCCCATGTACTCTGCGT65020.232492778850094No Hit
GATACCACTGCTTCCCATGTACTCT64740.2314915795563686No Hit
CCATGTACTCTGCGTTGATACCACT60710.2170814611502493No Hit
ACCATGTACTCTGCGTTGATACCAC60110.21493603409226625No Hit
GTTGATACCACTGCTTCCCATGTAC59230.2117894077405578No Hit
ATGGGAAGCAGTGGTATCAACGCAG58010.20742703938932566No Hit
GGTATCAACGCAGAGTACTTTTTTT57870.20692643974246297No Hit
TATCAACGCAGAGTACTTTTTTTTT53670.19190845033658174No Hit
GTACATGGGTAAGCAGTGGTATCAA51010.18239705704619033No Hit
TTCCCATGTACTCTGCGTTGATACC42770.15293319211655676No Hit
GGTAAGCAGTGGTATCAACGCAGAG42240.15103806488200508No Hit
TGATACCACTGCTTCCCATGTACTC40370.14435148388462465No Hit
CCCCATGTACTCTGCGTTGATACCA39230.14027517247445692No Hit
ACGCAGAGTACTTTTTTTTTTTTTT38240.13673521782878492No Hit
GCTTACCATGTACTCTGCGTTGATA36870.131836492713057No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA36500.13051347936063412No Hit
GTACTTTTTTTTTTTTTTTTTTTTT34590.1236838698927215No Hit
ATCAACGCAGAGTACATGGGAAGCA34500.12336205583402406No Hit
GGAAGCAGTGGTATCAACGCAGAGT29530.10559076837039798No Hit
GAGTACATGGTAAGCAGTGGTATCA28050.10029871496070651No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCCG250.0059723819.033395
TTAACGC604.0336515E-412.6889274
TGGCGCG855.363098E-511.16888814
CTTTGCG803.7964387E-410.6802499
GGCGCGA909.5861265E-510.54839315
ATTAACG750.002610399610.1511423
TCAGGAC3100.09.8236853
TTACACT3950.09.6371584
CGTCAGA800.0044523929.5166952
CTAGAAC2357.2759576E-129.3142123
ACTGTCC3300.09.2058728
TACACTG5800.09.1885335
GTGCTAT2304.0017767E-119.1058641
GTCCTAA2201.6189006E-109.0870491
ACGTACT1054.4161084E-49.06351954
CTAGACT1804.1032763E-88.9879894
GCGCGAT850.0074793758.93511116
GTCTAAG2252.5102054E-108.8851151
TAGAACT3000.08.8822494
CGAGACC1502.6397302E-68.8822494
Produced by FastQC (version 0.11.5)