FastQCFastQC Report
Wed 25 May 2016
SRR1294776_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294776_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2935166
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC39660.13512012608486199No Hit
GTATCAACGCAGAGTACTTTTTTTT39520.13464315135838995No Hit
CCCATGTACTCTGCGTTGATACCAC33090.11273638356399604No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGTA550.003074432512.088355
TGGATCG658.0414145E-411.6898335
TCGTATC600.00588180911.082312
ACCGTCT600.005896000711.0787228
GTGCGAC600.005896000711.07872211
TGCGCTG1752.0008883E-1110.8548455
TAGACAG4950.010.5533225
TGTGCGA1002.4118675E-510.44547410
CGGGGAA1303.8130383E-710.23697816
GCGGGAC750.0026515510.1320513
CGCTCTG1856.511982E-109.7546915
GTCTCGC1201.6999007E-59.5007421
GTCTAGA2800.09.5007421
CTTAGAC2401.8189894E-129.4987983
TCGCTCT1502.6566522E-79.4984754
TATACTG7700.09.4979895
CTCCGTG900.00111838249.4960487
GTCCTAA2650.09.3214821
CTGCGCT1951.6898412E-99.2549254
GTTAGCC3200.09.2019613
Produced by FastQC (version 0.11.5)