##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294776_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2935166
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.168418753828576	33.0	33.0	33.0	27.0	33.0
2	31.150458611199504	33.0	33.0	33.0	27.0	33.0
3	31.131966641750417	33.0	33.0	33.0	27.0	33.0
4	34.5713080623038	37.0	37.0	37.0	27.0	37.0
5	34.8891561840114	37.0	37.0	37.0	33.0	37.0
6	35.11055456488662	37.0	37.0	37.0	33.0	37.0
7	35.02863722188115	37.0	37.0	37.0	33.0	37.0
8	34.938268568115056	37.0	37.0	37.0	33.0	37.0
9	34.639908611642404	37.0	37.0	37.0	27.0	37.0
10	34.71799039645458	37.0	37.0	37.0	27.0	37.0
11	34.969295774072066	37.0	37.0	37.0	33.0	37.0
12	34.895050910238126	37.0	37.0	37.0	33.0	37.0
13	34.83396169075275	37.0	37.0	37.0	33.0	37.0
14	36.02294146225461	40.0	37.0	40.0	27.0	40.0
15	36.345719799152754	40.0	37.0	40.0	27.0	40.0
16	36.391239882173615	40.0	37.0	40.0	27.0	40.0
17	36.32632873234427	40.0	37.0	40.0	27.0	40.0
18	36.326601630027056	40.0	37.0	40.0	27.0	40.0
19	36.40073133853417	40.0	37.0	40.0	27.0	40.0
20	36.2904615956985	40.0	37.0	40.0	27.0	40.0
21	36.23699852069696	40.0	37.0	40.0	27.0	40.0
22	36.095442983463286	40.0	37.0	40.0	27.0	40.0
23	35.710481451474976	37.0	37.0	40.0	27.0	40.0
24	36.0232893131087	37.0	37.0	40.0	27.0	40.0
25	34.94245163646622	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.3810822564103482
1101	2	0.6350722575206049
1101	3	0.4528471045359801
1101	4	0.5163344132514496
1101	5	0.5263282299440704
1101	6	0.40400056700413245
1101	7	0.4093852742253006
1101	8	0.5162012778320957
1101	9	0.7544847668389991
1101	10	0.6516787661493879
1101	11	0.5778128939875415
1101	12	0.5551760382524193
1101	13	0.5067819349279787
1101	14	1.09423655736812
1101	15	0.961691569492082
1101	16	0.8237892290636921
1101	17	0.9593883978651121
1101	18	0.9300065311675993
1101	19	0.8379534904123389
1101	20	0.9718645049948833
1101	21	1.0643516727658593
1101	22	1.0235622440342524
1101	23	1.2858890202352313
1101	24	1.0786747162009576
1101	25	0.9202744143170278
1102	1	0.23359065685925273
1102	2	0.42162956747962
1102	3	0.22289211514021545
1102	4	0.16470118452414084
1102	5	0.19848058841947847
1102	6	0.2661344775159975
1102	7	0.26901797511940373
1102	8	0.2810683384616226
1102	9	0.4923809709488438
1102	10	0.4855192036056337
1102	11	0.33496508217194076
1102	12	0.43352006774599516
1102	13	0.3301103838277797
1102	14	0.907764310184433
1102	15	0.7282101771982497
1102	16	0.43380984186039484
1102	17	0.7484319566522615
1102	18	0.5873961064066506
1102	19	0.5402173681304276
1102	20	0.6961568699868579
1102	21	0.637293795468878
1102	22	0.717412159874641
1102	23	1.168343061309976
1102	24	0.862413330660857
1102	25	0.8013064219957116
1103	1	0.28266147174641176
1103	2	0.5192925071125742
1103	3	0.23421425891340775
1103	4	0.06671301874882829
1103	5	0.4099423590547815
1103	6	0.2639827760156308
1103	7	0.12693742535232388
1103	8	0.39406004766590996
1103	9	0.5769221376331828
1103	10	0.6447025080047482
1103	11	0.37805267098138273
1103	12	0.4589530192046283
1103	13	0.3900660569069885
1103	14	1.0188725125880893
1103	15	0.711025548387461
1103	16	0.456887541658098
1103	17	0.5890067694836176
1103	18	0.6565739106774089
1103	19	0.6555807572566295
1103	20	0.7875041449924751
1103	21	0.7949488709066515
1103	22	0.6760037263266909
1103	23	1.2288796003001181
1103	24	0.8318971012753238
1103	25	0.7945861947461097
1104	1	0.285890233137966
1104	2	0.5415746329161522
1104	3	0.36563195218763767
1104	4	0.4373202103838807
1104	5	0.3958915809581143
1104	6	0.28048761972558367
1104	7	0.26594490364627177
1104	8	0.4914485442854044
1104	9	0.6678806845315535
1104	10	0.6529274829902434
1104	11	0.40667634865746294
1104	12	0.3755727107119071
1104	13	0.43973574171975827
1104	14	1.0300633899805902
1104	15	0.757618407582747
1104	16	0.7317485059126838
1104	17	0.7597052866333698
1104	18	0.7202752554225853
1104	19	0.777988179596818
1104	20	0.8397877182240663
1104	21	0.9199282226582852
1104	22	0.9044254076626075
1104	23	1.3685900970304061
1104	24	1.1554013636164129
1104	25	0.9836095669858551
1105	1	0.16162559650349806
1105	2	0.21331927227651448
1105	3	0.2960392149250062
1105	4	0.2477431054392909
1105	5	0.3294952622894769
1105	6	0.17752201642891663
1105	7	0.13652528960617616
1105	8	0.34287500234521673
1105	9	-1.7710158054092986
1105	10	-0.1508834910488659
1105	11	0.05299099533327478
1105	12	0.17248399025726968
1105	13	0.17123350686587457
1105	14	-1.437306767150595
1105	15	-0.17696825878023503
1105	16	-0.12705106293323354
1105	17	0.1976983269258099
1105	18	0.21399313510326579
1105	19	0.22672482330125376
1105	20	0.36343295498553374
1105	21	0.5423911117196241
1105	22	0.5173744187937643
1105	23	0.9389144694374849
1105	24	0.7792465414855485
1105	25	0.2876100702279132
1106	1	0.09530140499011708
1106	2	0.15647211553746487
1106	3	0.13542934707189502
1106	4	-1.0845156988195086
1106	5	-0.2001338852856165
1106	6	-0.06678105814442858
1106	7	-0.05803017003036359
1106	8	0.08054647234101253
1106	9	0.4132763786666729
1106	10	0.39129611361722283
1106	11	0.31040746831731525
1106	12	0.033067030299768874
1106	13	0.12466485467560062
1106	14	0.5878628545595674
1106	15	0.4275056992072379
1106	16	0.24323594151593397
1106	17	0.11178735452835298
1106	18	0.22105301070368455
1106	19	0.2115850840534108
1106	20	0.3643178300325758
1106	21	0.467308304096278
1106	22	0.5475403882029752
1106	23	0.6871688466648322
1106	24	0.46576720465485977
1106	25	0.32254997327592605
1107	1	0.29998379309518697
1107	2	0.23468335020231024
1107	3	0.23420111602922944
1107	4	0.46563109652316115
1107	5	0.48046707556277113
1107	6	0.22975870561182887
1107	7	0.23234861362676895
1107	8	0.2775904366427113
1107	9	0.3962495373924071
1107	10	0.5371096009564837
1107	11	0.2339041458758473
1107	12	0.14442277217097654
1107	13	0.2644437413410827
1107	14	0.7700987436820483
1107	15	0.4922412126025435
1107	16	0.5068804445006379
1107	17	0.42583786239329413
1107	18	0.47006586986086774
1107	19	0.4734019824888662
1107	20	0.5000114392931962
1107	21	0.6700835854694915
1107	22	0.45178612222190395
1107	23	0.8564628341856277
1107	24	0.6531354763942616
1107	25	0.531413081932854
1108	1	0.5797204232206603
1108	2	0.5113383842992221
1108	3	0.586008366385169
1108	4	0.8038250918473153
1108	5	0.5967374993963972
1108	6	0.5650794856589414
1108	7	0.49427662277486917
1108	8	0.6312102697613966
1108	9	0.3633838671287819
1108	10	0.5978400523344547
1108	11	0.4639226469008264
1108	12	0.49334610510318555
1108	13	0.5337651294194643
1108	14	-0.8283176372725549
1108	15	0.19760165654749784
1108	16	0.504095957486264
1108	17	0.7106504833602187
1108	18	0.7963495581342173
1108	19	0.7542733512722677
1108	20	0.7771989051878805
1108	21	0.8474522536408529
1108	22	0.967801760298201
1108	23	1.3057671386512482
1108	24	1.1251854514371047
1108	25	1.1319497323675165
1109	1	0.515788064200855
1109	2	0.420419178433562
1109	3	0.4947371172162178
1109	4	0.6552859734390992
1109	5	0.5859331648288872
1109	6	0.40486004533322983
1109	7	0.33104460229748156
1109	8	0.39385615972451404
1109	9	0.5540713405881732
1109	10	0.5421017268674788
1109	11	0.41797236153774264
1109	12	0.40825023315432674
1109	13	0.41616147287871996
1109	14	0.743273037229855
1109	15	0.5841563888807713
1109	16	0.515170702127314
1109	17	0.469955592266011
1109	18	0.5551775247368056
1109	19	0.5250932589482247
1109	20	0.5582837990372056
1109	21	0.661998137402982
1109	22	0.7307654507795291
1109	23	1.1505728515644478
1109	24	0.8578748895885511
1109	25	0.7875680805348111
1110	1	0.41840680543451825
1110	2	0.28027258570137903
1110	3	0.37724843057429425
1110	4	0.6056200046551936
1110	5	0.49106451744520285
1110	6	0.2714574373310441
1110	7	0.2756009767187422
1110	8	0.18074368356054293
1110	9	0.4102894852908321
1110	10	0.3765641392508172
1110	11	0.2660750044651863
1110	12	0.1615170995362334
1110	13	0.19197712724185578
1110	14	0.5154050566012387
1110	15	0.414956831142959
1110	16	0.2804051683217139
1110	17	0.32629802982542344
1110	18	0.4268747992587336
1110	19	0.31946361430813397
1110	20	0.43388714700677866
1110	21	0.5393858489389629
1110	22	0.5134531180519417
1110	23	0.6637047047139788
1110	24	0.5288845846181474
1110	25	0.48567366502873455
1111	1	0.23445115650653037
1111	2	0.16195384616739972
1111	3	0.32533485402250406
1111	4	0.5493716930791024
1111	5	0.4212405155508492
1111	6	0.34133493865583375
1111	7	0.2952234721910614
1111	8	0.4414448176733643
1111	9	0.3707715633129709
1111	10	0.2714538833746616
1111	11	0.22920104112709794
1111	12	0.3084357245169329
1111	13	0.2539910074945766
1111	14	-1.5558247300791734
1111	15	-0.3450740580409928
1111	16	0.07525685318930897
1111	17	0.17502501065730058
1111	18	0.25796503680548
1111	19	0.27640492242885273
1111	20	0.3590411345808633
1111	21	0.46762641347944367
1111	22	0.2973690510526339
1111	23	-1.9187183688702731
1111	24	-0.30717571025604684
1111	25	0.21409195723944663
1112	1	-0.04670544846684521
1112	2	-0.3074574685517639
1112	3	0.02794349072572544
1112	4	0.18432956087275443
1112	5	-0.7499139027552104
1112	6	-0.8169980842816358
1112	7	-0.8132859652329429
1112	8	-0.6572574650372047
1112	9	-0.6318940842776257
1112	10	-0.9184796036440801
1112	11	-0.7401748067616367
1112	12	-0.694557776526814
1112	13	-0.7045948375907471
1112	14	-0.5327816391581024
1112	15	-0.7941082227171066
1112	16	-0.7162630842181272
1112	17	-0.8439473763726753
1112	18	-0.9069706404419193
1112	19	-0.7776595574839149
1112	20	-0.8636136353977832
1112	21	-1.717569475921195
1112	22	-1.6302081839934885
1112	23	-1.3578322955779925
1112	24	-1.468478310362272
1112	25	-1.8725666026832215
1113	1	-0.11692375106929376
1113	2	-0.29420325697216043
1113	3	-0.14390633014223653
1113	4	0.10564856417347812
1113	5	-0.1028267651497643
1113	6	-0.13502649594788352
1113	7	-0.11368475853578275
1113	8	-0.018598776516938642
1113	9	0.2107023192086359
1113	10	-0.1592614908409331
1113	11	-0.25872438693608757
1113	12	-0.12720969113717473
1113	13	-0.21659160893445772
1113	14	-0.1496245052261358
1113	15	-0.29666999618392964
1113	16	-0.11623185404026515
1113	17	-0.044275643247353
1113	18	0.0313244225331033
1113	19	-0.25862792209329655
1113	20	-0.7689142859177736
1113	21	-0.7808312285625973
1113	22	-0.18504299894394904
1113	23	-0.43009058267194433
1113	24	-0.14926214168940533
1113	25	-1.2044080042897818
1114	1	-1.070569714743808
1114	2	-0.8291863286606116
1114	3	-0.6444716599495415
1114	4	-1.571961698449961
1114	5	-0.8999655556477748
1114	6	-0.7444478171983846
1114	7	-0.6288095491877925
1114	8	-0.6350215247025943
1114	9	-0.5927764668617854
1114	10	-0.7163467171292695
1114	11	-0.6482940745503925
1114	12	-0.6733233983772422
1114	13	-0.7661625658713334
1114	14	-0.8347494883820445
1114	15	-1.5455082050795355
1114	16	-0.9930627465845845
1114	17	-1.0254631997953751
1114	18	-0.9648455018609923
1114	19	-0.8962328667965522
1114	20	-2.1453770147567823
1114	21	-6.946987058061893
1114	22	-7.693468804332131
1114	23	-8.024047524362512
1114	24	-6.028814558094588
1114	25	-7.352305440619119
1115	1	-1.0255511489397442
1115	2	-0.9782204769905398
1115	3	-0.7103376805804196
1115	4	-0.5787561350250385
1115	5	-0.5755559827226762
1115	6	-0.8407624217517267
1115	7	-1.0377680979378638
1115	8	-0.7864420470365303
1115	9	-2.7688948599035683
1115	10	-1.5712119286314703
1115	11	-1.0974203281588615
1115	12	-1.0398167276712726
1115	13	-1.3331447936976417
1115	14	-0.9961222642285463
1115	15	-1.9823126453742006
1115	16	-1.322908789895635
1115	17	-2.9883678518612697
1115	18	-2.8246397969043358
1115	19	-2.1567531844943346
1115	20	-1.7336851345838582
1115	21	-1.344947582992532
1115	22	-0.9929023045988217
1115	23	-0.3287759998436002
1115	24	-1.3932826532179448
1115	25	-1.0258096637620113
1116	1	-1.5586122988184385
1116	2	-0.8505975331383375
1116	3	-0.6954654666080451
1116	4	-0.37476968029019986
1116	5	-0.41029993856432867
1116	6	-0.49016485026974976
1116	7	-0.8521152672010075
1116	8	-1.894635040534581
1116	9	-1.001730521253947
1116	10	-2.432155445387366
1116	11	-1.2803864055745677
1116	12	-0.9574582629329811
1116	13	-0.8753498444312413
1116	14	-1.2373619229832187
1116	15	-1.4549265366890012
1116	16	-0.9176860610708459
1116	17	-0.8281050666610099
1116	18	-1.33483625750506
1116	19	-0.9337881500276453
1116	20	-1.2736297128074412
1116	21	-0.7520748885186421
1116	22	-0.37909001918654894
1116	23	-0.21373190660888497
1116	24	-0.42563694861996737
1116	25	-0.41034194277041536
1201	1	0.29309170115817906
1201	2	0.3767305100851033
1201	3	0.26736035036265093
1201	4	0.3602328468533784
1201	5	0.39201558678974635
1201	6	0.26825785387852363
1201	7	0.23700880437660032
1201	8	0.36319361281420015
1201	9	0.5859584853745119
1201	10	0.6229987914506268
1201	11	0.39864359177525444
1201	12	0.4073582700259024
1201	13	0.34067577187642684
1201	14	1.092668980668833
1201	15	0.7627645148469071
1201	16	0.7042251646791087
1201	17	0.5706981298055709
1201	18	0.5255108469144787
1201	19	0.589791169613072
1201	20	0.7319113358576814
1201	21	0.7297211926316578
1201	22	0.8025712526893187
1201	23	0.7683392329777803
1201	24	0.8115934340096516
1201	25	0.6237156184045176
1202	1	-0.1659423300309406
1202	2	0.05060426374076954
1202	3	0.1241986577884795
1202	4	-0.02817883646677899
1202	5	0.2878896932176147
1202	6	0.07997682010051932
1202	7	0.05035878219990053
1202	8	0.15650064938144936
1202	9	0.26215753215186766
1202	10	0.48717108550140864
1202	11	0.37391653906058053
1202	12	0.33866707993872325
1202	13	0.41110408185912917
1202	14	0.6684729173063033
1202	15	0.6424581931768785
1202	16	0.6131193054858031
1202	17	0.515426510355887
1202	18	0.4432910139767472
1202	19	0.44974738792137003
1202	20	0.6286830486378747
1202	21	0.6592656145956894
1202	22	0.571281583105673
1202	23	0.846631939326798
1202	24	0.661487147880301
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2211	7	0.26177382739065536
2211	8	0.28779119924531216
2211	9	0.48479816184802615
2211	10	0.37770414530184837
2211	11	0.12650178534779855
2211	12	0.25852894427232087
2211	13	0.14177013237662095
2211	14	0.4340074617254501
2211	15	0.24591594144726514
2211	16	0.20201866234120303
2211	17	0.1803514145817715
2211	18	0.2617360723229112
2211	19	0.27621509234299424
2211	20	0.3759770360747581
2211	21	0.352327738562515
2211	22	0.4521247116809164
2211	23	0.5166790244303314
2211	24	0.3914645427777259
2211	25	0.5895040121448574
2212	1	-0.31967028542967313
2212	2	-0.3694249240597003
2212	3	-0.18666677864490566
2212	4	-0.5447168042782025
2212	5	-0.3199676263701008
2212	6	-0.633816383633409
2212	7	-0.6185204034193603
2212	8	-0.3247941099017453
2212	9	-0.33071223018994544
2212	10	-0.6787888280678871
2212	11	-0.5495210263689785
2212	12	-0.7672687495404986
2212	13	-0.6549094272293132
2212	14	-0.5346868457756955
2212	15	-0.6411256808038956
2212	16	-0.5082569972696191
2212	17	-0.6490918346293526
2212	18	-0.493501970979878
2212	19	-0.6724109808243313
2212	20	-0.579637278506901
2212	21	-0.48602782524498167
2212	22	-0.5931326212594428
2212	23	-0.08600542488449037
2212	24	-0.27145356347065075
2212	25	-0.8116224213236336
2213	1	-0.26861113640707046
2213	2	-1.4158982715298514
2213	3	-0.4150871626755901
2213	4	-0.03749177659131675
2213	5	-0.24229554383178709
2213	6	-0.16443877203712276
2213	7	-0.0977858962800795
2213	8	0.015714008854345707
2213	9	-0.0876159857557326
2213	10	-0.4515422798540172
2213	11	-0.20297365167528625
2213	12	-0.18975427834263314
2213	13	-0.5289668042751501
2213	14	-0.2279604712023442
2213	15	-0.2099331947870624
2213	16	-0.23696583979132413
2213	17	-0.008532379142515367
2213	18	-0.02547348638623248
2213	19	-0.08966335686926641
2213	20	-0.1470622611712855
2213	21	-0.6155129351895852
2213	22	-1.1187146242523056
2213	23	-0.26323854211905484
2213	24	-0.17521308074732644
2213	25	-0.6158126342989618
2214	1	-0.2210884955040271
2214	2	-0.419535962262124
2214	3	-0.43561842025137665
2214	4	-0.5043146112498462
2214	5	-0.29871165102942854
2214	6	-0.3761097884858202
2214	7	-0.48779947890466246
2214	8	-0.19939210930568407
2214	9	-0.04880751311789311
2214	10	-1.9453429350977771
2214	11	-0.8785642593841274
2214	12	-0.4671714678612915
2214	13	-0.3937334846371101
2214	14	-0.7393644294495516
2214	15	-0.6515414363328276
2214	16	-0.708244958323867
2214	17	-0.6049221831417242
2214	18	-0.5349797968957688
2214	19	-1.4895928306107322
2214	20	-2.420111466657332
2214	21	-1.9312735231110167
2214	22	-2.147753413552479
2214	23	-2.574169089660444
2214	24	-1.6434324162752176
2214	25	-2.0139041434685865
2215	1	-0.3329648916320984
2215	2	-0.42586507618617375
2215	3	-0.932640013698883
2215	4	-0.48234886292519974
2215	5	-0.3757779205752172
2215	6	-0.4265565494686925
2215	7	-0.3712693225383177
2215	8	-0.3788411040934747
2215	9	-0.3241239688140922
2215	10	-0.4377107683070065
2215	11	-0.631728816346147
2215	12	-0.5498222496930083
2215	13	-0.4830856812119464
2215	14	-0.31661684940487334
2215	15	-0.766582389422922
2215	16	-0.649421428912099
2215	17	-0.5058901652712819
2215	18	-0.4996112190848976
2215	19	-2.2809182215265906
2215	20	-2.371898510588828
2215	21	-3.526396382381307
2215	22	-2.4980884842896316
2215	23	-3.583476610136991
2215	24	-2.4163289166475153
2215	25	-1.815653957318176
2216	1	-1.4839378467328892
2216	2	-1.1073751659415834
2216	3	-0.8136981006909316
2216	4	-0.9032228424580637
2216	5	-1.8275052888519667
2216	6	-1.4930156057869226
2216	7	-1.1277210134829403
2216	8	-4.990525839222272
2216	9	-4.0506273517785765
2216	10	-2.0095937233809167
2216	11	-1.025549429177488
2216	12	-1.2632167460923114
2216	13	-1.997219403064257
2216	14	-3.4839295095143896
2216	15	-2.0047347558852024
2216	16	-3.0297810150533735
2216	17	-2.6774971876442777
2216	18	-2.6818571741776225
2216	19	-1.571519171498501
2216	20	-0.9269640866067448
2216	21	-0.7675754066574711
2216	22	-0.8177056461477505
2216	23	-0.9991405286770316
2216	24	-0.5609915744721548
2216	25	-0.3327054692358331
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	6.0
7	34.0
8	246.0
9	574.0
10	1257.0
11	2512.0
12	4386.0
13	6461.0
14	8628.0
15	10928.0
16	12296.0
17	13367.0
18	13980.0
19	14372.0
20	15272.0
21	16603.0
22	17946.0
23	19234.0
24	20971.0
25	22887.0
26	24879.0
27	28816.0
28	32613.0
29	38801.0
30	46823.0
31	57642.0
32	77712.0
33	110914.0
34	160147.0
35	255525.0
36	446282.0
37	1453052.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.241619374917356	20.28608157128115	12.881202398690236	23.591096655111258
2	15.932031745415154	19.87673112590183	40.29078723954527	23.900449889137747
3	19.17004425222062	24.584074789183457	28.891152350179343	27.35472860841658
4	12.541654952675477	16.12260928088873	37.698298702515736	33.63743706392005
5	13.45706122738289	37.59258804895138	35.18696856436386	13.763382159301878
6	32.66572465469309	37.75017011073468	16.51619348567032	13.067911748901903
7	27.735093796303207	32.000857200192016	22.063092796009183	18.200956207495594
8	24.72571070933461	37.47120656624866	19.940949824726733	17.862132899689996
9	24.12826204351833	17.115245384716285	21.392361720359034	37.364130851406344
10	14.709540875375495	30.243577028471318	33.44605978135885	21.60082231479434
11	33.6475828588814	24.97672173159654	22.39221216289098	18.98348324663108
12	21.105003735860205	26.879216045153314	31.190463454496953	20.825316764489532
13	27.40186678986553	23.10346660343185	26.24749070897948	23.24717589772314
14	20.523428460847633	22.60131346017305	27.748447437744193	29.126810641235128
15	23.03649224204691	30.723204560499635	23.447412828858273	22.792890368595188
16	22.301151608132294	30.67571844763728	25.178723953728525	21.8444059905019
17	20.671668411633096	29.85650618086074	27.849527905096537	21.62229750240962
18	21.07698104876407	29.51471698524622	28.50248985387951	20.905812112110205
19	21.436040004701624	28.55157564080943	28.772279487795366	21.24010486669358
20	22.483480429483283	28.25257950602268	28.304399597295543	20.959540467198494
21	22.623448896709053	28.22273144217228	27.78438172400506	21.36943793711361
22	21.71391082469815	28.011612614157798	28.75219978497432	21.522276776169726
23	21.938025679303998	28.257771000404414	28.343359587319796	21.460843732971792
24	21.744863089539475	27.87307546854824	28.425589326537498	21.956472115374787
25	22.15123416214075	27.963961133752548	27.897681581557826	21.987123122548876
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1637.0
1	1637.0
2	1868.5
3	2100.0
4	2100.0
5	2100.0
6	3346.5
7	4593.0
8	4593.0
9	4593.0
10	7524.5
11	10456.0
12	10456.0
13	10456.0
14	17679.0
15	24902.0
16	24902.0
17	24902.0
18	38976.0
19	53050.0
20	53050.0
21	53050.0
22	76015.5
23	98981.0
24	98981.0
25	98981.0
26	129233.0
27	159485.0
28	159485.0
29	159485.0
30	192374.5
31	225264.0
32	225264.0
33	225264.0
34	253667.0
35	282070.0
36	282070.0
37	282070.0
38	302491.0
39	322912.0
40	322912.0
41	322912.0
42	327473.5
43	332035.0
44	332035.0
45	332035.0
46	335904.0
47	339773.0
48	339773.0
49	339773.0
50	326124.0
51	312475.0
52	312475.0
53	312475.0
54	289663.0
55	266851.0
56	266851.0
57	266851.0
58	237687.5
59	208524.0
60	208524.0
61	208524.0
62	176754.5
63	144985.0
64	144985.0
65	144985.0
66	113692.5
67	82400.0
68	82400.0
69	82400.0
70	60413.5
71	38427.0
72	38427.0
73	38427.0
74	27187.5
75	15948.0
76	15948.0
77	15948.0
78	10828.0
79	5708.0
80	5708.0
81	5708.0
82	3781.0
83	1854.0
84	1854.0
85	1854.0
86	1189.0
87	524.0
88	524.0
89	524.0
90	345.0
91	166.0
92	166.0
93	166.0
94	102.0
95	38.0
96	38.0
97	38.0
98	23.0
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03059452174084873
2	0.01509284313050778
3	0.02125944495132473
4	0.02183862854775505
5	0.022554090637463095
6	0.010663792098981795
7	9.88019076263489E-4
8	0.001192436816180073
9	0.002759639488873883
10	0.001260576062818934
11	8.517405829857665E-4
12	0.003372892708623635
13	0.002521152125637868
14	6.132532197497518E-4
15	0.0016694115426521023
16	0.0018056900359298246
17	0.0019419685292075474
18	0.0018397596592492555
19	3.747658565137372E-4
20	3.747658565137372E-4
21	0.08776334967085338
22	0.08493557093534063
23	0.08626428624479841
24	0.0012265064394995037
25	0.2269377609307276
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2935166.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.91024082965488
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.43740108933777	67.24831575300097
2	9.038582591114567	13.903191276826949
3	1.8008338759487874	4.155077012802663
4	0.621616575044258	1.912347219614365
5	0.28406690397007633	1.092382699803651
6	0.17003414706460238	0.7846420320002113
7	0.11759460505125875	0.6330960576332322
8	0.08496131833210323	0.5227516363301625
9	0.06070170414062646	0.42017244158034356
>10	0.34452693731624817	4.905052959576317
>50	0.02688768420413895	1.4500085468556658
>100	0.011459292830714587	1.575854288685924
>500	6.221953013685537E-4	0.3307680886106853
>1k	7.110803435714834E-4	1.0663399866787848
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	3966	0.13512012608486199	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3952	0.13464315135838995	No Hit
CCCATGTACTCTGCGTTGATACCAC	3309	0.11273638356399604	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.406962331943066E-5	0.0
5	0.0	0.0	0.0	3.406962331943066E-5	0.0
6	0.0	0.0	0.0	3.406962331943066E-5	0.0
7	0.0	0.0	0.0	3.406962331943066E-5	0.0
8	0.0	0.0	0.0	3.406962331943066E-5	0.0
9	0.0	0.0	0.0	1.0220886995829196E-4	0.0
10	0.0	0.0	0.0	1.7034811659715328E-4	0.0
11	0.0	0.0	0.0	1.7034811659715328E-4	0.0
12	0.0	0.0	0.0	2.0441773991658393E-4	0.0
13	0.0	0.0	0.0	2.384873632360146E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGCGTA	55	0.0030744325	12.08835	5
TGGATCG	65	8.0414145E-4	11.689833	5
TCGTATC	60	0.005881809	11.08231	2
ACCGTCT	60	0.0058960007	11.078722	8
GTGCGAC	60	0.0058960007	11.078722	11
TGCGCTG	175	2.0008883E-11	10.854845	5
TAGACAG	495	0.0	10.553322	5
TGTGCGA	100	2.4118675E-5	10.445474	10
CGGGGAA	130	3.8130383E-7	10.236978	16
GCGGGAC	75	0.00265155	10.132051	3
CGCTCTG	185	6.511982E-10	9.754691	5
GTCTCGC	120	1.6999007E-5	9.500742	1
GTCTAGA	280	0.0	9.500742	1
CTTAGAC	240	1.8189894E-12	9.498798	3
TCGCTCT	150	2.6566522E-7	9.498475	4
TATACTG	770	0.0	9.497989	5
CTCCGTG	90	0.0011183824	9.496048	7
GTCCTAA	265	0.0	9.321482	1
CTGCGCT	195	1.6898412E-9	9.254925	4
GTTAGCC	320	0.0	9.201961	3
>>END_MODULE