FastQCFastQC Report
Wed 25 May 2016
SRR1294776_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294776_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2935166
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT108920.3710863371952387No Hit
GGTATCAACGCAGAGTACTTTTTTT85200.2902731906815492No Hit
TATCAACGCAGAGTACTTTTTTTTT70750.24104258498497186No Hit
ACGCAGAGTACTTTTTTTTTTTTTT44730.15239342510781334No Hit
GTACATGGGAAGCAGTGGTATCAAC41790.14237695585190072No Hit
CCCATGTACTCTGCGTTGATACCAC37550.1279314355644621No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG500.001506267713.2927627
CGCGCGC751.4884685E-512.65934110
TCGTATA701.07685366E-412.2320192
CGTAGAC550.003036776312.1082563
TCGCGCG550.003082888712.0839189
AGGGCCG1354.629328E-911.27541355
GTCTTAG2900.011.1581011
ATCGCGC951.36946255E-510.9940138
GTCGTAT803.710268E-410.7068541
TCTACAC4400.010.3785063
GCGCGCC1106.0918737E-610.35764311
GTTCCGG750.00260930710.1516851
GTATCGC856.617309E-410.0554056
TGTGCGG856.6318345E-410.05300710
CTAGGAC2850.010.0143483
TACACCG951.6213243E-410.0143485
CATTGCG951.6540132E-49.9945577
TCTAGAC2600.09.87953953
TGTATCG1106.73379E-59.513635
TGCACCG800.0044643529.513635
Produced by FastQC (version 0.11.5)