FastQCFastQC Report
Wed 25 May 2016
SRR1294775_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294775_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3710014
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1044892.81640446639824No Hit
CCCATGTACTCTGCGTTGATACCAC1012202.7282915913524852No Hit
GAGTACATGGGAAGCAGTGGTATCA501291.3511808850317006No Hit
CATGTACTCTGCGTTGATACCACTG399551.07695011393488No Hit
GCGTTGATACCACTGCTTCCCATGT326710.880616623010048No Hit
GCTTCCCATGTACTCTGCGTTGATA300650.8103743004743378No Hit
ACGCAGAGTACATGGGAAGCAGTGG272520.7345524841685234No Hit
GTATCAACGCAGAGTACATGGGAAG263120.709215652555489No Hit
ACTCTGCGTTGATACCACTGCTTCC218050.5877336311938446No Hit
TATCAACGCAGAGTACATGGGAAGC205720.554499255258875No Hit
GGTATCAACGCAGAGTACATGGGAA178900.4822084229331749No Hit
CAGTGGTATCAACGCAGAGTACATG146070.39371819082084325No Hit
GTGGTATCAACGCAGAGTACATGGG139330.37555114347277396No Hit
GCAGTGGTATCAACGCAGAGTACAT118750.3200796546859392No Hit
GTACATGGTAAGCAGTGGTATCAAC114080.30749210110797426No Hit
ATACCACTGCTTCCCATGTACTCTG110080.29671047063434264No Hit
ACATGGGAAGCAGTGGTATCAACGC104950.2828830295519101No Hit
CATGGGAAGCAGTGGTATCAACGCA103540.2790825048099549No Hit
GTACTCTGCGTTGATACCACTGCTT101560.2737455977255072No Hit
CTGCTTCCCATGTACTCTGCGTTGA97460.2626944264900348No Hit
GGGAAGCAGTGGTATCAACGCAGAG95260.2567645297295374No Hit
CTTCCCATGTACTCTGCGTTGATAC84840.228678382345727No Hit
GATACCACTGCTTCCCATGTACTCT83040.22382664863259275No Hit
TACCACTGCTTCCCATGTACTCTGC82920.22350319971838384No Hit
ACCATGTACTCTGCGTTGATACCAC82330.22191290922352314No Hit
AAGCAGTGGTATCAACGCAGAGTAC82310.221859001071155No Hit
GCAGAGTACATGGGAAGCAGTGGTA80070.21582128800592126No Hit
GTTGATACCACTGCTTCCCATGTAC79860.2152552524060556No Hit
CCACTGCTTCCCATGTACTCTGCGT78990.2129102477780407No Hit
CCATGTACTCTGCGTTGATACCACT76780.20695339694135925No Hit
GTACATGGGTAAGCAGTGGTATCAA74920.20193993877112054No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA71770.19344940477313563No Hit
ATGGGAAGCAGTGGTATCAACGCAG67310.18142788679503635No Hit
TTCCCATGTACTCTGCGTTGATACC63170.1702688992548276No Hit
TGATACCACTGCTTCCCATGTACTC56520.152344438592415No Hit
GGTAAGCAGTGGTATCAACGCAGAG52950.14272183339469877No Hit
CCCCATGTACTCTGCGTTGATACCA48540.1308350857975199No Hit
GCTTACCATGTACTCTGCGTTGATA46240.12463564827518171No Hit
ATCAACGCAGAGTACATGGGAAGCA40840.110080447135779No Hit
GAGTACATGGTAAGCAGTGGTATCA38190.10293761694699805No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCGT550.003080857212.0851189
GTATCGA1303.2385287E-810.96900518
CTAGACT2700.010.9057134
GTACAAA6450.010.459971
GTCTTAG3650.010.1532141
CTAGGGC1701.5643309E-910.0578023
GATACGA1201.688196E-59.50647118
GGTATCG1502.6252928E-79.50647117
CCCGTCA900.00111930989.49532212
GTCCTAA2357.2759576E-129.3001891
GACTCCG1651.07484084E-79.2077097
GTTCTAG3000.09.1856211
CCTAGAC2900.09.1714833
GTCTAGG2600.09.1368911
CGCAAAA3050.09.0319922
TAGACTG3600.08.9705865
ATGGGTA21100.08.8680515
ACCGTGT2151.0513759E-98.83309658
ATACGAC1304.2574018E-58.78908119
GAACAAA13950.08.7871351
Produced by FastQC (version 0.11.5)