##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294775_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3710014
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.817312010143358	33.0	33.0	33.0	27.0	33.0
2	30.812883724967076	33.0	33.0	33.0	27.0	33.0
3	30.83142732075944	33.0	33.0	33.0	27.0	33.0
4	34.149379220671406	37.0	37.0	37.0	27.0	37.0
5	34.544882040876395	37.0	37.0	37.0	27.0	37.0
6	34.5968290146614	37.0	37.0	37.0	27.0	37.0
7	34.653305890489904	37.0	37.0	37.0	27.0	37.0
8	34.54409767725944	37.0	37.0	37.0	27.0	37.0
9	34.26413620002512	37.0	37.0	37.0	27.0	37.0
10	34.37721340135105	37.0	37.0	37.0	27.0	37.0
11	34.63135637763092	37.0	37.0	37.0	27.0	37.0
12	34.56562724561147	37.0	37.0	37.0	27.0	37.0
13	34.429683553754785	37.0	37.0	37.0	27.0	37.0
14	35.615904683917634	40.0	37.0	40.0	27.0	40.0
15	35.84675529526304	40.0	37.0	40.0	27.0	40.0
16	35.85181646214812	37.0	37.0	40.0	27.0	40.0
17	35.832906560460415	37.0	37.0	40.0	27.0	40.0
18	35.821623314629	37.0	37.0	40.0	27.0	40.0
19	35.82528691266394	40.0	37.0	40.0	27.0	40.0
20	35.75253462655397	40.0	37.0	40.0	27.0	40.0
21	35.674580742821995	37.0	37.0	40.0	27.0	40.0
22	35.57909242390999	37.0	37.0	40.0	27.0	40.0
23	35.24239423355276	37.0	33.0	40.0	27.0	40.0
24	35.57081347941005	37.0	37.0	40.0	27.0	40.0
25	34.432723704007586	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.3276833284780274
1101	2	0.40037263957326985
1101	3	0.2242449558602715
1101	4	0.33754036088649997
1101	5	0.3626507024289225
1101	6	0.26815723730792485
1101	7	0.2772006909422231
1101	8	0.33008349738791765
1101	9	0.5484036220000945
1101	10	0.5275725229943617
1101	11	0.3143661941874001
1101	12	0.3799916846885125
1101	13	0.3796162253584825
1101	14	0.9804444562723873
1101	15	0.7309967544436518
1101	16	0.7200313709794344
1101	17	0.6859765834360374
1101	18	0.6844470322726579
1101	19	0.6134145187427933
1101	20	0.7883690694199075
1101	21	0.7935566247903125
1101	22	0.6797910793560078
1101	23	1.0759234008557854
1101	24	0.90214494530872
1101	25	0.6337054071091401
1102	1	0.25342398508053066
1102	2	0.4166752116582515
1102	3	0.25160534637145915
1102	4	0.20869336528103588
1102	5	0.2135604915924887
1102	6	0.12054406836587361
1102	7	0.14076856584800623
1102	8	0.21753370637974712
1102	9	0.4194309818236164
1102	10	0.5230493940363132
1102	11	0.2876849798651335
1102	12	0.30818195683525573
1102	13	0.3049012502946198
1102	14	0.8259111593270916
1102	15	0.6252638617987003
1102	16	0.3294463223809956
1102	17	0.5044648569874042
1102	18	0.5605180917618071
1102	19	0.5185650011741032
1102	20	0.7020340126946394
1102	21	0.7100414268623183
1102	22	0.6862765164547255
1102	23	1.04884359387367
1102	24	0.805220175562603
1102	25	0.7381803042763906
1103	1	0.2938824748674449
1103	2	0.5077391157628739
1103	3	0.26322780642121657
1103	4	0.033985638089710335
1103	5	0.19968620997160258
1103	6	0.16005577037859098
1103	7	0.2757059523466978
1103	8	0.43640197159872685
1103	9	0.50553153935833
1103	10	0.5250899385169845
1103	11	0.43955502262376456
1103	12	0.38790675639953065
1103	13	0.3814062018007931
1103	14	0.9691263896302189
1103	15	0.7542224396485722
1103	16	0.5015722509271541
1103	17	0.5907122354494234
1103	18	0.6934786499181413
1103	19	0.6870015359042085
1103	20	0.8320739888362354
1103	21	0.9179048567590229
1103	22	0.7077430088702101
1103	23	1.0412038734846547
1103	24	0.9159594899739929
1103	25	0.812695032990689
1104	1	0.28239822351534016
1104	2	0.4935060616788114
1104	3	0.32353993934873415
1104	4	0.2950760860442685
1104	5	0.2771082851556699
1104	6	0.1306724237440804
1104	7	0.18933853670743162
1104	8	0.343179180099888
1104	9	0.4662803638094317
1104	10	0.5926223937207027
1104	11	0.35537002179563615
1104	12	0.2731792752547406
1104	13	0.30058335388969937
1104	14	0.8739366847366341
1104	15	0.6737167847950332
1104	16	0.6090251119251135
1104	17	0.7262968951430935
1104	18	0.6549047234391807
1104	19	0.6832836779962008
1104	20	0.7256035237494416
1104	21	0.7251686676741329
1104	22	0.819445055813631
1104	23	1.1993874507736422
1104	24	0.908531541270591
1104	25	0.7569223818428554
1105	1	0.17145022960568923
1105	2	0.331610019748684
1105	3	0.27383788093426986
1105	4	0.1367283088098219
1105	5	0.2135743752890562
1105	6	0.20319892039242404
1105	7	0.24500016470751973
1105	8	0.23789103802003808
1105	9	-1.5847067213067945
1105	10	-0.19342361193584878
1105	11	-0.04676961863933826
1105	12	0.20054729763300827
1105	13	0.22733925633547614
1105	14	-1.6029184812080928
1105	15	-0.28192453056324496
1105	16	-0.028685727481722267
1105	17	0.11922170790470688
1105	18	0.04239357669691657
1105	19	0.23149415744305912
1105	20	0.35208141250243585
1105	21	0.4849241204729964
1105	22	0.49542746333005994
1105	23	0.8423372019499311
1105	24	0.6664541830988426
1105	25	0.15805756372146362
1106	1	0.05680390749502706
1106	2	0.014349486492861274
1106	3	0.009823139225083821
1106	4	-1.1473336417157896
1106	5	-0.2385458790000854
1106	6	-0.10755039135803912
1106	7	-0.13981794728640295
1106	8	0.047147714456230005
1106	9	0.28632444858262573
1106	10	0.2775210111516344
1106	11	0.19725418971029285
1106	12	0.018168348829718184
1106	13	0.08355029696146943
1106	14	0.5469326208224743
1106	15	0.32818634474084973
1106	16	0.04897841011693771
1106	17	0.006650969462093315
1106	18	0.1298593803935688
1106	19	0.10453054031680153
1106	20	0.29342748276548747
1106	21	0.32379349768577015
1106	22	0.387053622622922
1106	23	0.5521488893675723
1106	24	0.34679528196483744
1106	25	0.404081910079924
1107	1	0.23020210043306477
1107	2	0.27681199887423347
1107	3	0.16329430577071946
1107	4	0.20074044348028508
1107	5	0.24953788216230777
1107	6	0.027103629705678145
1107	7	0.145072943545415
1107	8	0.09750872457691884
1107	9	0.1966433786143753
1107	10	0.26604554383236234
1107	11	0.09228786478136897
1107	12	0.12750470326506047
1107	13	0.033033064024152736
1107	14	0.567210294767186
1107	15	0.409070171950475
1107	16	0.3656285179857335
1107	17	0.35384541774636347
1107	18	0.31844164483098325
1107	19	0.4296112795962159
1107	20	0.5214100231383085
1107	21	0.4071867779840872
1107	22	0.3965871061085977
1107	23	0.6237162136380476
1107	24	0.5435104252254135
1107	25	0.34941198558112774
1108	1	0.4186714868491883
1108	2	0.3708301793802171
1108	3	0.4259820321249066
1108	4	0.6349144311193768
1108	5	0.4964267422557356
1108	6	0.37605772573233764
1108	7	0.3260451675901379
1108	8	0.4742691204671132
1108	9	0.16305192496121634
1108	10	0.4114936923207111
1108	11	0.3301332189436579
1108	12	0.35230475706518405
1108	13	0.3456511618616531
1108	14	-1.0889027445462673
1108	15	-0.10992430697562128
1108	16	0.3547327622907943
1108	17	0.5165514328628902
1108	18	0.6181284395244546
1108	19	0.5317983039243686
1108	20	0.5996937171074421
1108	21	0.7582998501025031
1108	22	0.8085566499885033
1108	23	1.0734946981803546
1108	24	0.9284679592113463
1108	25	0.748344430337589
1109	1	0.41040725218809015
1109	2	0.3349019650371403
1109	3	0.40722262098613626
1109	4	0.5901737519112231
1109	5	0.59793716728786
1109	6	0.4115534041374147
1109	7	0.3378255291786374
1109	8	0.3617225667006565
1109	9	0.5212617004992879
1109	10	0.5574735048006332
1109	11	0.366197581094319
1109	12	0.39403324445677157
1109	13	0.36616192318513185
1109	14	0.6772008432307928
1109	15	0.480254856010248
1109	16	0.5207498930729955
1109	17	0.4527042597180113
1109	18	0.48576729774000427
1109	19	0.5581925327631083
1109	20	0.6679501969901906
1109	21	0.6856955495885302
1109	22	0.7668700735349248
1109	23	1.136361983556199
1109	24	0.8775909665595591
1109	25	0.7129709712547054
1110	1	0.4128858314098274
1110	2	0.24017569644817627
1110	3	0.398237212246471
1110	4	0.5885563241814395
1110	5	0.393909312517728
1110	6	0.27745425123251977
1110	7	0.34980619586517747
1110	8	0.31298371673220515
1110	9	0.4681011430874591
1110	10	0.4406887786780871
1110	11	0.2489857781101037
1110	12	0.21512467121659995
1110	13	0.18409066277747144
1110	14	0.5445851868492255
1110	15	0.3639585741376905
1110	16	0.3821317637408441
1110	17	0.3798037576433302
1110	18	0.45857524671836103
1110	19	0.38446678079201746
1110	20	0.43628282953249453
1110	21	0.45020149799611175
1110	22	0.522537596769034
1110	23	0.7871472244711128
1110	24	0.6968899300987559
1110	25	0.5738849650273039
1111	1	0.12718879399783134
1111	2	0.046080997413042724
1111	3	0.24585265471808526
1111	4	0.5315319811946395
1111	5	0.385021672983207
1111	6	0.3222555027173968
1111	7	0.21717669251220428
1111	8	0.3004927492951097
1111	9	0.39162661618128425
1111	10	0.2509739119718404
1111	11	0.1708471436005894
1111	12	0.15939770574640022
1111	13	0.1969936334764384
1111	14	-1.5370129956006906
1111	15	-0.476490969965198
1111	16	-0.03930551309735364
1111	17	0.17938867441609574
1111	18	0.22102683894032538
1111	19	0.19183752580466518
1111	20	0.3031561317703364
1111	21	0.41171646232915293
1111	22	0.23038091094145585
1111	23	-1.7430246402015754
1111	24	-0.19117110230968848
1111	25	0.039036937147365336
1112	1	-0.1708360950695429
1112	2	-0.2785012273595946
1112	3	0.08461674611171688
1112	4	0.23427404825281428
1112	5	-0.6685323214826511
1112	6	-0.9698662767148747
1112	7	-0.9704667477777562
1112	8	-0.7892484175718621
1112	9	-0.5574080269078294
1112	10	-0.8209001109116798
1112	11	-0.8467876524040108
1112	12	-0.8209539615868522
1112	13	-0.8215872600937502
1112	14	-0.500677222203457
1112	15	-0.8557321408240242
1112	16	-0.8440833946634285
1112	17	-0.9746042275144688
1112	18	-0.9498649628015059
1112	19	-0.8011442965887312
1112	20	-0.8671297278783641
1112	21	-1.8176436385412558
1112	22	-1.8193836077449816
1112	23	-1.3941156804719768
1112	24	-1.5655446677700837
1112	25	-1.7552241497993393
1113	1	0.10340640070520024
1113	2	-0.09609385583264185
1113	3	0.021362938105365714
1113	4	0.2614444298697265
1113	5	0.16695833636649837
1113	6	0.07600731202409605
1113	7	0.09087257289925077
1113	8	0.1063239325903993
1113	9	0.29557145193548706
1113	10	0.0038079021566730376
1113	11	-0.15073850436483838
1113	12	0.050366779551644925
1113	13	-0.046298516329798645
1113	14	0.1282517240135448
1113	15	0.008623701833975872
1113	16	0.027302340391393898
1113	17	0.10981981462560952
1113	18	0.09525188680732555
1113	19	0.03725450608180125
1113	20	-0.5365697816574979
1113	21	-0.455896326323014
1113	22	0.032665222975559516
1113	23	-0.21502435652527652
1113	24	0.10564398087139182
1113	25	-0.7873447751656286
1114	1	-0.9496261977614218
1114	2	-0.7189481230554016
1114	3	-0.5794095139889599
1114	4	-1.6389599919724631
1114	5	-0.6784497557662874
1114	6	-0.531907848848796
1114	7	-0.5280340336586988
1114	8	-0.44809128230204465
1114	9	-0.42450843223704027
1114	10	-0.4641765392182293
1114	11	-0.5202215310951246
1114	12	-0.5981453767387279
1114	13	-0.6202191453055832
1114	14	-0.5096227162341407
1114	15	-1.4108249381810225
1114	16	-0.9234975598081832
1114	17	-0.8115105435565937
1114	18	-0.8930055243678794
1114	19	-0.8067807324935643
1114	20	-2.0109508444423554
1114	21	-6.7308819099037045
1114	22	-7.772014149732254
1114	23	-7.60999300252551
1114	24	-5.7263706761827
1114	25	-7.14037956031553
1115	1	-1.1474623330150955
1115	2	-1.0492989225068001
1115	3	-0.8329243062308116
1115	4	-0.5698661750837601
1115	5	-0.6715879581725588
1115	6	-0.9255803293161122
1115	7	-1.1634564003421062
1115	8	-0.6916361689644077
1115	9	-2.7300258189092936
1115	10	-1.6982765631734864
1115	11	-1.2068193519241746
1115	12	-0.9832588673431601
1115	13	-1.4548682388727698
1115	14	-1.0687960915569263
1115	15	-1.9942795687441688
1115	16	-1.3678166154667792
1115	17	-3.048660324163677
1115	18	-2.9338068070774526
1115	19	-2.221761695281522
1115	20	-1.8902927710123123
1115	21	-1.382942221880576
1115	22	-0.8282752716894919
1115	23	-0.30750588234944587
1115	24	-1.2934869808987486
1115	25	-0.9565336398600479
1116	1	-1.3204419323589995
1116	2	-0.6780232960221433
1116	3	-0.42093858819917784
1116	4	-0.0849828370900596
1116	5	-0.15868820972877984
1116	6	-0.15404011798063522
1116	7	-0.6086180053837467
1116	8	-1.5531253563380218
1116	9	-0.6591067603414373
1116	10	-2.2488511126720354
1116	11	-0.8430526958631788
1116	12	-0.49253585624502705
1116	13	-0.5516051913911895
1116	14	-0.8649307696630828
1116	15	-1.059338398076072
1116	16	-0.5701339751940608
1116	17	-0.5427956530940605
1116	18	-0.976177267295725
1116	19	-0.5717703619174941
1116	20	-0.7979956730185549
1116	21	-0.32179234112075505
1116	22	0.15319825614007954
1116	23	0.24649699807285685
1116	24	0.1600609410551499
1116	25	0.3665331802294247
1201	1	0.28555787666904564
1201	2	0.36376663640437457
1201	3	0.3472267190575984
1201	4	0.32189648888017075
1201	5	0.3332383891102282
1201	6	0.2565167114710647
1201	7	0.20313684691215883
1201	8	0.29694386255970073
1201	9	0.6131140016614012
1201	10	0.5191564009158824
1201	11	0.4280821240372248
1201	12	0.3920671200382344
1201	13	0.37424353722821735
1201	14	1.0659000719248937
1201	15	0.7604703982855128
1201	16	0.7353384846933935
1201	17	0.7765840749519839
1201	18	0.6274380353385851
1201	19	0.5980019045911718
1201	20	0.7651491244353323
1201	21	0.730092590292422
1201	22	0.7473123667180133
1201	23	0.5746781375568375
1201	24	0.726420573633213
1201	25	0.6614084118869528
1202	1	-0.2004625619723619
1202	2	-0.043973236725282305
1202	3	-0.02408504489529406
1202	4	-0.03412185184919281
1202	5	-0.055422040712834075
1202	6	-0.12557974270200134
1202	7	-0.1164373836163719
1202	8	-0.028004045217507212
1202	9	0.11473454982093045
1202	10	0.2533278323319337
1202	11	0.025158559151293503
1202	12	0.02573611929862807
1202	13	0.21535514614896556
1202	14	0.33483550601489753
1202	15	0.31356624086388507
1202	16	0.2547112801078555
1202	17	0.12438322298634574
1202	18	0.15084931143803715
1202	19	0.11836054087722658
1202	20	0.35252805001989884
1202	21	0.2656388652540187
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2210	25	0.8816731417072887
2211	1	0.37164060610193417
2211	2	0.29001325676063416
2211	3	0.2806598684533945
2211	4	0.4812361713187556
2211	5	0.29734137896712554
2211	6	0.27232218501544736
2211	7	0.2075609010289199
2211	8	0.2620163912686664
2211	9	0.44677089370841117
2211	10	0.3591127366011264
2211	11	0.2008110337713518
2211	12	0.21868305603464222
2211	13	0.12371810972129538
2211	14	0.4531414713665569
2211	15	0.24779834541826062
2211	16	0.252566896294951
2211	17	0.3370207484272214
2211	18	0.2934141780486428
2211	19	0.2883120508768613
2211	20	0.4276207111657442
2211	21	0.47497478990027986
2211	22	0.4638360761603906
2211	23	0.5662652319048149
2211	24	0.5610312720347892
2211	25	0.6746515733683367
2212	1	-0.2802178114180016
2212	2	-0.34308341959390276
2212	3	-0.22357842409662965
2212	4	-0.6751716453516039
2212	5	-0.2800597204082962
2212	6	-0.5910093430782553
2212	7	-0.5217676262200612
2212	8	-0.3108548661976229
2212	9	-0.2663216196245415
2212	10	-0.6313220780223574
2212	11	-0.5186308628709497
2212	12	-0.632492052726306
2212	13	-0.602919904007372
2212	14	-0.5499580785526845
2212	15	-0.6440915509318117
2212	16	-0.4385013776610478
2212	17	-0.50481464324114
2212	18	-0.36215928248200413
2212	19	-0.5263646794120049
2212	20	-0.6049532383246472
2212	21	-0.5119555738569375
2212	22	-0.5504673893885084
2212	23	0.013850762670827521
2212	24	-0.2510228925059934
2212	25	-0.9966355992467442
2213	1	-0.03990835148258398
2213	2	-1.2308140821786715
2213	3	-0.20849193597100069
2213	4	0.13868095427620375
2213	5	0.12304305844599384
2213	6	0.1199506254121232
2213	7	0.1764221249862601
2213	8	0.22881909020313884
2213	9	0.2033286451267955
2213	10	-0.318003681546827
2213	11	0.009673670169839
2213	12	0.08091129603737812
2213	13	-0.22401456759789085
2213	14	0.050354274744577765
2213	15	-0.050741751719449724
2213	16	-0.013735465411485848
2213	17	0.10389718419060046
2213	18	0.0939935256303599
2213	19	0.1075186598678215
2213	20	0.2810622103517417
2213	21	-0.37077846526769775
2213	22	-0.83640049231078
2213	23	0.024258985707689362
2213	24	0.13559012318354036
2213	25	-0.4194748302450435
2214	1	-0.14583367551825432
2214	2	-0.3205424105594652
2214	3	-0.3389624103168245
2214	4	-0.4137353498513505
2214	5	-0.21333778607777987
2214	6	-0.14848998620877296
2214	7	-0.35389535291166396
2214	8	0.12426657424642684
2214	9	0.13456568736549457
2214	10	-1.7733656759472751
2214	11	-0.7698461452677492
2214	12	-0.3751966957464532
2214	13	-0.14016557452838896
2214	14	-0.7842311516392897
2214	15	-0.48069885645200117
2214	16	-0.6535819512382091
2214	17	-0.4631582827769236
2214	18	-0.4998697521702411
2214	19	-1.334562651578338
2214	20	-2.4695317529607266
2214	21	-1.7826821419823915
2214	22	-1.9930460683545377
2214	23	-2.468878621639732
2214	24	-1.5505494178490835
2214	25	-1.782490329386043
2215	1	-0.1889165651669309
2215	2	-0.28795326234838825
2215	3	-0.8566649715200327
2215	4	-0.24482355478752993
2215	5	-0.21614620546608876
2215	6	-0.15981061272297126
2215	7	-0.23700845446260388
2215	8	-0.15798880030053652
2215	9	-0.15793368501319094
2215	10	-0.2970105507501586
2215	11	-0.6223011759656742
2215	12	-0.43607463575111893
2215	13	-0.27965853891974746
2215	14	-0.2087898101143537
2215	15	-0.6268075195767011
2215	16	-0.394640550946022
2215	17	-0.22932120864654593
2215	18	-0.294289785430621
2215	19	-2.1563817721905565
2215	20	-2.345783258256027
2215	21	-3.29886865945398
2215	22	-2.405523377033738
2215	23	-3.296275112885155
2215	24	-2.3601455703735894
2215	25	-1.7083021929879862
2216	1	-1.3777892398328753
2216	2	-1.0847339519959611
2216	3	-0.6757061757173126
2216	4	-0.732843329842737
2216	5	-1.5643895400233632
2216	6	-1.3199717159827742
2216	7	-1.0040826805778096
2216	8	-4.948787543619545
2216	9	-3.848802047168089
2216	10	-1.8040796764690228
2216	11	-0.9332041783124083
2216	12	-0.9773158266464392
2216	13	-1.7395769404492398
2216	14	-3.3133929965240014
2216	15	-1.872178890217704
2216	16	-2.734646706053958
2216	17	-2.5023427487694647
2216	18	-2.3019519896440386
2216	19	-1.3482662781231127
2216	20	-0.6589068614026914
2216	21	-0.5404163070339081
2216	22	-0.457860291270336
2216	23	-0.7323961906272345
2216	24	-0.3255624431615942
2216	25	-0.05929781318004501
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	5.0
7	101.0
8	563.0
9	1442.0
10	2607.0
11	4414.0
12	7131.0
13	10065.0
14	13957.0
15	18935.0
16	22458.0
17	24516.0
18	24973.0
19	25573.0
20	26378.0
21	28050.0
22	29459.0
23	30252.0
24	31998.0
25	33932.0
26	35956.0
27	39532.0
28	44305.0
29	51703.0
30	61446.0
31	74399.0
32	98653.0
33	139313.0
34	201258.0
35	315549.0
36	549137.0
37	1761954.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.402685164538724	19.968976680742113	11.60392394029049	24.024414214428674
2	13.25791191621009	22.46761530224819	37.636555969750916	26.637916811790802
3	20.56984011057758	28.003766798202655	27.889889360412656	23.53650373080711
4	12.758409749656055	18.825011586128134	34.90353790072568	33.51304076349012
5	11.805255065686298	39.82882119992311	35.210492984915156	13.15543074947543
6	31.848204256778196	34.59819003768666	18.102884763899734	15.450720941635415
7	24.58131624060637	33.58494431206805	23.008819502097058	18.82491994522852
8	25.938932873816402	36.553994991351715	21.452820673302277	16.05425146152961
9	25.886591730337027	17.75183463908567	21.112460020150976	35.24911361042633
10	15.664832260605158	31.941522545350942	32.667989394101525	19.72565579994237
11	29.77471742979647	27.505422560817994	20.49017739530017	22.22968261408537
12	22.2608450014798	30.731399115569502	29.458311926981384	17.549443955969313
13	27.877814726892076	23.14243995873751	23.87981693341639	25.099928380954022
14	20.73895476243743	26.093149001204864	26.66881403353666	26.499082202821043
15	28.4576449544519	27.06982676734079	22.136248613179138	22.336279665028176
16	19.57321887236648	30.155170838330086	29.78987905639785	20.481731232905588
17	23.83673148130428	30.381601940530683	27.60088983218744	18.1807767459776
18	23.896496736310727	27.523941268687775	28.860334153478572	19.719227841522926
19	22.194132860375447	30.956701981274858	29.258824496594997	17.5903406617547
20	22.822079275039453	28.380280835958	27.93699182882996	20.860648060172586
21	19.795355532600865	30.06612947651342	29.760719366119577	20.37779562476614
22	21.208084578221666	29.591074516874883	24.676426148765067	24.524414756138388
23	18.58025920296787	30.508224927446154	27.910017583416018	23.001498286169962
24	20.64972633142873	33.52764799208186	25.898890608583052	19.923735067906357
25	21.714803336121356	27.780731500152367	23.995689366383324	26.50877579734295
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9542.0
1	9542.0
2	11099.5
3	12657.0
4	12657.0
5	12657.0
6	15144.0
7	17631.0
8	17631.0
9	17631.0
10	19746.5
11	21862.0
12	21862.0
13	21862.0
14	28302.0
15	34742.0
16	34742.0
17	34742.0
18	49320.5
19	63899.0
20	63899.0
21	63899.0
22	86911.5
23	109924.0
24	109924.0
25	109924.0
26	139398.5
27	168873.0
28	168873.0
29	168873.0
30	199309.5
31	229746.0
32	229746.0
33	229746.0
34	256587.0
35	283428.0
36	283428.0
37	283428.0
38	304216.5
39	325005.0
40	325005.0
41	325005.0
42	358879.5
43	392754.0
44	392754.0
45	392754.0
46	585878.5
47	779003.0
48	779003.0
49	779003.0
50	677360.5
51	575718.0
52	575718.0
53	575718.0
54	434644.0
55	293570.0
56	293570.0
57	293570.0
58	233744.0
59	173918.0
60	173918.0
61	173918.0
62	143165.5
63	112413.0
64	112413.0
65	112413.0
66	86335.0
67	60257.0
68	60257.0
69	60257.0
70	43988.5
71	27720.0
72	27720.0
73	27720.0
74	19543.0
75	11366.0
76	11366.0
77	11366.0
78	7675.5
79	3985.0
80	3985.0
81	3985.0
82	2692.5
83	1400.0
84	1400.0
85	1400.0
86	920.0
87	440.0
88	440.0
89	440.0
90	284.0
91	128.0
92	128.0
93	128.0
94	76.0
95	24.0
96	24.0
97	24.0
98	16.5
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.032102304735238195
2	0.014716925596507184
3	0.020565960128452347
4	0.022560561766074197
5	0.02137458241397472
6	0.005741218227208846
7	0.001455520113940271
8	8.355763617064518E-4
9	5.390815236815818E-4
10	8.086222855223726E-5
11	0.0013746578853880336
12	0.001509428266308429
13	0.0028840861516964624
14	0.00118597935209948
15	0.002425866856567118
16	0.002776269846960146
17	0.002722361694591988
18	0.002749315770776067
19	7.816682093382936E-4
20	5.121274474975027E-4
21	0.08611827340813269
22	0.08234470274236162
23	0.08388108508485413
24	0.0014016119615721128
25	0.2283010252791499
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3710014.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.16160594879226
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.89434548124254	57.41032832259627
2	6.8247379165977184	8.62122813983853
3	1.1444200084383216	2.1685021683868437
4	0.36289263478804723	0.9168352640080648
5	0.1838823129044874	0.5807151094312876
6	0.10298242082036378	0.390272105010511
7	0.08155025106201212	0.36055913758227204
8	0.057630486256432405	0.2912027250852857
9	0.04647603583724919	0.2641950955452853
>10	0.25577906187853683	3.013698731612872
>50	0.023393850386953643	1.0233481370258959
>100	0.015815808113270036	1.935411977199955
>500	0.0022041157125100917	0.9654730584480673
>1k	0.002333769576952347	3.0253982294885624
>5k	7.347052371887017E-4	3.57729209952429
>10k+	8.21141147446196E-4	15.455539699215986
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	104489	2.81640446639824	No Hit
CCCATGTACTCTGCGTTGATACCAC	101220	2.7282915913524852	No Hit
GAGTACATGGGAAGCAGTGGTATCA	50129	1.3511808850317006	No Hit
CATGTACTCTGCGTTGATACCACTG	39955	1.07695011393488	No Hit
GCGTTGATACCACTGCTTCCCATGT	32671	0.880616623010048	No Hit
GCTTCCCATGTACTCTGCGTTGATA	30065	0.8103743004743378	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	27252	0.7345524841685234	No Hit
GTATCAACGCAGAGTACATGGGAAG	26312	0.709215652555489	No Hit
ACTCTGCGTTGATACCACTGCTTCC	21805	0.5877336311938446	No Hit
TATCAACGCAGAGTACATGGGAAGC	20572	0.554499255258875	No Hit
GGTATCAACGCAGAGTACATGGGAA	17890	0.4822084229331749	No Hit
CAGTGGTATCAACGCAGAGTACATG	14607	0.39371819082084325	No Hit
GTGGTATCAACGCAGAGTACATGGG	13933	0.37555114347277396	No Hit
GCAGTGGTATCAACGCAGAGTACAT	11875	0.3200796546859392	No Hit
GTACATGGTAAGCAGTGGTATCAAC	11408	0.30749210110797426	No Hit
ATACCACTGCTTCCCATGTACTCTG	11008	0.29671047063434264	No Hit
ACATGGGAAGCAGTGGTATCAACGC	10495	0.2828830295519101	No Hit
CATGGGAAGCAGTGGTATCAACGCA	10354	0.2790825048099549	No Hit
GTACTCTGCGTTGATACCACTGCTT	10156	0.2737455977255072	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	9746	0.2626944264900348	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	9526	0.2567645297295374	No Hit
CTTCCCATGTACTCTGCGTTGATAC	8484	0.228678382345727	No Hit
GATACCACTGCTTCCCATGTACTCT	8304	0.22382664863259275	No Hit
TACCACTGCTTCCCATGTACTCTGC	8292	0.22350319971838384	No Hit
ACCATGTACTCTGCGTTGATACCAC	8233	0.22191290922352314	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	8231	0.221859001071155	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	8007	0.21582128800592126	No Hit
GTTGATACCACTGCTTCCCATGTAC	7986	0.2152552524060556	No Hit
CCACTGCTTCCCATGTACTCTGCGT	7899	0.2129102477780407	No Hit
CCATGTACTCTGCGTTGATACCACT	7678	0.20695339694135925	No Hit
GTACATGGGTAAGCAGTGGTATCAA	7492	0.20193993877112054	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	7177	0.19344940477313563	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	6731	0.18142788679503635	No Hit
TTCCCATGTACTCTGCGTTGATACC	6317	0.1702688992548276	No Hit
TGATACCACTGCTTCCCATGTACTC	5652	0.152344438592415	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	5295	0.14272183339469877	No Hit
CCCCATGTACTCTGCGTTGATACCA	4854	0.1308350857975199	No Hit
GCTTACCATGTACTCTGCGTTGATA	4624	0.12463564827518171	No Hit
ATCAACGCAGAGTACATGGGAAGCA	4084	0.110080447135779	No Hit
GAGTACATGGTAAGCAGTGGTATCA	3819	0.10293761694699805	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.390815236815818E-5	0.0
5	0.0	2.695407618407909E-5	0.0	5.390815236815818E-5	0.0
6	0.0	2.695407618407909E-5	0.0	5.390815236815818E-5	0.0
7	0.0	2.695407618407909E-5	0.0	5.390815236815818E-5	0.0
8	0.0	2.695407618407909E-5	0.0	5.390815236815818E-5	0.0
9	0.0	2.695407618407909E-5	0.0	8.086222855223728E-5	0.0
10	2.695407618407909E-5	2.695407618407909E-5	0.0	1.3477038092039544E-4	0.0
11	2.695407618407909E-5	2.695407618407909E-5	0.0	1.3477038092039544E-4	0.0
12	2.695407618407909E-5	2.695407618407909E-5	0.0	1.6172445710447455E-4	0.0
13	2.695407618407909E-5	2.695407618407909E-5	0.0	1.8867853328855363E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTCGT	55	0.0030808572	12.085118	9
GTATCGA	130	3.2385287E-8	10.969005	18
CTAGACT	270	0.0	10.905713	4
GTACAAA	645	0.0	10.45997	1
GTCTTAG	365	0.0	10.153214	1
CTAGGGC	170	1.5643309E-9	10.057802	3
GATACGA	120	1.688196E-5	9.506471	18
GGTATCG	150	2.6252928E-7	9.506471	17
CCCGTCA	90	0.0011193098	9.495322	12
GTCCTAA	235	7.2759576E-12	9.300189	1
GACTCCG	165	1.07484084E-7	9.207709	7
GTTCTAG	300	0.0	9.185621	1
CCTAGAC	290	0.0	9.171483	3
GTCTAGG	260	0.0	9.136891	1
CGCAAAA	305	0.0	9.031992	2
TAGACTG	360	0.0	8.970586	5
ATGGGTA	2110	0.0	8.868051	5
ACCGTGT	215	1.0513759E-9	8.8330965	8
ATACGAC	130	4.2574018E-5	8.789081	19
GAACAAA	1395	0.0	8.787135	1
>>END_MODULE