FastQCFastQC Report
Wed 25 May 2016
SRR1294775_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294775_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3710014
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1133183.0543820050274744No Hit
GTACATGGGAAGCAGTGGTATCAAC1037132.7954881032793946No Hit
GAGTACATGGGAAGCAGTGGTATCA539961.4554122976355344No Hit
CATGTACTCTGCGTTGATACCACTG460881.242259463171837No Hit
GCGTTGATACCACTGCTTCCCATGT353710.9533926287070615No Hit
ACGCAGAGTACATGGGAAGCAGTGG336200.906196041308739No Hit
GCTTCCCATGTACTCTGCGTTGATA334070.9004548230815302No Hit
GTATCAACGCAGAGTACATGGGAAG282190.7606170758385278No Hit
ACTCTGCGTTGATACCACTGCTTCC253160.6823693926761463No Hit
TATCAACGCAGAGTACATGGGAAGC239180.6446875941708037No Hit
GGTATCAACGCAGAGTACATGGGAA194970.52552362336099No Hit
CAGTGGTATCAACGCAGAGTACATG180230.4857933150656574No Hit
GTGGTATCAACGCAGAGTACATGGG151710.40892028978866385No Hit
GCAGTGGTATCAACGCAGAGTACAT135770.3659554923512418No Hit
CATGGGAAGCAGTGGTATCAACGCA135260.36458083446585376No Hit
ACATGGGAAGCAGTGGTATCAACGC130320.3512655208309187No Hit
ATACCACTGCTTCCCATGTACTCTG123660.33331410609232204No Hit
GGGAAGCAGTGGTATCAACGCAGAG111580.3007535820619545No Hit
GTACATGGTAAGCAGTGGTATCAAC111410.3002953627668251No Hit
CTGCTTCCCATGTACTCTGCGTTGA107430.28956764044556166No Hit
GTACTCTGCGTTGATACCACTGCTT103700.27951377002890015No Hit
CTTCCCATGTACTCTGCGTTGATAC101790.27436554147774106No Hit
AAGCAGTGGTATCAACGCAGAGTAC100340.2704572004310496No Hit
GATACCACTGCTTCCCATGTACTCT94910.2558211370630946No Hit
GCAGAGTACATGGGAAGCAGTGGTA93850.25296400498758226No Hit
TACCACTGCTTCCCATGTACTCTGC93470.25193975009258723No Hit
GTATCAACGCAGAGTACTTTTTTTT93360.2516432552545624No Hit
CCACTGCTTCCCATGTACTCTGCGT93060.25083463296904No Hit
CCATGTACTCTGCGTTGATACCACT90430.2437457109326272No Hit
ACCATGTACTCTGCGTTGATACCAC89840.24215542043776653No Hit
GTTGATACCACTGCTTCCCATGTAC87930.23700719188660743No Hit
ATGGGAAGCAGTGGTATCAACGCAG82430.22218244998536393No Hit
GTACATGGGTAAGCAGTGGTATCAA80100.21590215023447348No Hit
TATCAACGCAGAGTACTTTTTTTTT70850.19096962976420034No Hit
GGTATCAACGCAGAGTACTTTTTTT69630.1876812324697427No Hit
TTCCCATGTACTCTGCGTTGATACC66650.17964891776688713No Hit
GGTAAGCAGTGGTATCAACGCAGAG63400.17088884300706142No Hit
TGATACCACTGCTTCCCATGTACTC60510.16309911498986257No Hit
CCCCATGTACTCTGCGTTGATACCA56150.1513471377736041No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA54500.14689971520323106No Hit
GCTTACCATGTACTCTGCGTTGATA51600.1390830331098481No Hit
ATCAACGCAGAGTACATGGGAAGCA49380.13309922819698256No Hit
ACGCAGAGTACTTTTTTTTTTTTTT49030.13215583553053978No Hit
GTACTTTTTTTTTTTTTTTTTTTTT45150.12169765397111709No Hit
GAGTACATGGTAAGCAGTGGTATCA44100.11886747597178877No Hit
GGAAGCAGTGGTATCAACGCAGAGT37980.10237158134713238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCAG702.4594556E-816.310575
CTACGCA604.0420657E-412.6859994
ATACCGT752.0833648E-411.3952366
GACTCCG1053.4957138E-610.8504117
GTAGCAC2300.010.7555213
CGCAAAA1701.3460522E-1010.634142
CTCCGTG750.00266271410.1270517
CTACACT5250.09.7863424
GTCTTAG2950.09.6817771
CTAGCAC3150.09.6655243
TAGCACC2401.8189894E-129.5144994
TCTACGC1002.7152422E-49.5144993
CGAATAG800.00454161749.49398215
CTTAGAC2054.110916E-109.2824383
GCTACAC2700.09.162113
GTGTAAG5000.09.1395981
GCGATGA1252.7768901E-59.114223513
AGTACTC10750.09.027715
TATTCCG950.00179955189.0137365
TAAGACT3800.09.0137364
Produced by FastQC (version 0.11.5)