FastQCFastQC Report
Wed 25 May 2016
SRR1294774_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294774_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4266460
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1300453.048077328745611No Hit
CCCATGTACTCTGCGTTGATACCAC1289673.0228104798826196No Hit
GAGTACATGGGAAGCAGTGGTATCA607421.4237095859330686No Hit
CATGTACTCTGCGTTGATACCACTG506791.1878465988196303No Hit
GCGTTGATACCACTGCTTCCCATGT421660.9883134964349836No Hit
GTATCAACGCAGAGTACATGGGAAG338770.7940306483595299No Hit
ACGCAGAGTACATGGGAAGCAGTGG331240.7763813559719299No Hit
GCTTCCCATGTACTCTGCGTTGATA308660.7234569174444387No Hit
ACTCTGCGTTGATACCACTGCTTCC275880.6466250709018718No Hit
TATCAACGCAGAGTACATGGGAAGC248370.582145385167094No Hit
GGTATCAACGCAGAGTACATGGGAA210090.4924222892046333No Hit
CAGTGGTATCAACGCAGAGTACATG180300.42259859461942684No Hit
GTGGTATCAACGCAGAGTACATGGG167920.3935815641070114No Hit
GCAGTGGTATCAACGCAGAGTACAT147590.34593081852402224No Hit
ATACCACTGCTTCCCATGTACTCTG143730.3368835052947877No Hit
GTACATGGTAAGCAGTGGTATCAAC138080.3236406763452605No Hit
GTACTCTGCGTTGATACCACTGCTT129590.30374127496800624No Hit
CATGGGAAGCAGTGGTATCAACGCA125090.2931938890790023No Hit
CTGCTTCCCATGTACTCTGCGTTGA119700.2805604646475064No Hit
ACATGGGAAGCAGTGGTATCAACGC119430.2799276214941661No Hit
GGGAAGCAGTGGTATCAACGCAGAG113340.26565349259104737No Hit
TACCACTGCTTCCCATGTACTCTGC109600.25688744298551963No Hit
ACCATGTACTCTGCGTTGATACCAC105310.24683226843800246No Hit
CTTCCCATGTACTCTGCGTTGATAC103960.24366805267130126No Hit
CCATGTACTCTGCGTTGATACCACT100440.23541765304256923No Hit
AAGCAGTGGTATCAACGCAGAGTAC99410.23300347360575277No Hit
GCAGAGTACATGGGAAGCAGTGGTA99020.23208936682870576No Hit
GATACCACTGCTTCCCATGTACTCT99010.2320659281933969No Hit
GTACATGGGTAAGCAGTGGTATCAA98510.23089399642795197No Hit
GTTGATACCACTGCTTCCCATGTAC98490.23084711915733416No Hit
CCACTGCTTCCCATGTACTCTGCGT96710.2266750420723504No Hit
ATGGGAAGCAGTGGTATCAACGCAG80590.18889196195440716No Hit
TTCCCATGTACTCTGCGTTGATACC77320.18122752820839758No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA76880.1801962282548061No Hit
TGATACCACTGCTTCCCATGTACTC71520.1676331197292369No Hit
GGTAAGCAGTGGTATCAACGCAGAG67180.15746075200517526No Hit
CCCCATGTACTCTGCGTTGATACCA59460.13936612554670616No Hit
ATCAACGCAGAGTACATGGGAAGCA51220.1200526900521744No Hit
GCTTACCATGTACTCTGCGTTGATA49850.1168415970148554No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA49810.11674784247361983No Hit
GAGTACATGGTAAGCAGTGGTATCA49040.1149430675548347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAA9250.012.4280861
TACGACC1155.347829E-912.3891644
GTCGTAC1302.6175258E-911.6933041
CGACCAT1157.1288014E-811.5601026
GTCTTAG2750.011.4009711
TCGTACC1001.9335512E-611.3992332
ACGCACG600.005899954611.07791211
GTACGAC1605.456968E-1010.686283
GTATCGA1553.6307028E-910.42611118
GGTATCG1659.2040864E-1010.37035617
GTCTAGC1502.4456313E-810.1341961
AGCGTTC750.002659190410.1287328
TGGTCGA750.002659707610.12849510
ATACGAC1605.9462764E-910.117866519
TAGACAG4600.010.1176995
GAACAAA17450.010.0724921
ACCGCTC1252.5879035E-69.8755148
ACACCGT1451.6299236E-79.8232396
TATCGAC1804.0217856E-99.52269719
CGTACCC1201.7043487E-59.4989163
Produced by FastQC (version 0.11.5)