##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294774_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4266460
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.88749056594929	33.0	33.0	33.0	27.0	33.0
2	30.87342972862748	33.0	33.0	33.0	27.0	33.0
3	30.83809411080849	33.0	33.0	33.0	27.0	33.0
4	34.09116269694314	37.0	33.0	37.0	27.0	37.0
5	34.56193893766729	37.0	37.0	37.0	27.0	37.0
6	34.69590316093436	37.0	37.0	37.0	27.0	37.0
7	34.72270055268302	37.0	37.0	37.0	27.0	37.0
8	34.62274977381717	37.0	37.0	37.0	27.0	37.0
9	34.29662975862893	37.0	37.0	37.0	27.0	37.0
10	34.39558931760757	37.0	37.0	37.0	27.0	37.0
11	34.682679551665785	37.0	37.0	37.0	27.0	37.0
12	34.613399633419746	37.0	37.0	37.0	27.0	37.0
13	34.485821031956235	37.0	37.0	37.0	27.0	37.0
14	35.66227059435691	37.0	37.0	40.0	27.0	40.0
15	35.89984319552978	40.0	37.0	40.0	27.0	40.0
16	35.90162898515397	37.0	37.0	40.0	27.0	40.0
17	35.9089305888254	37.0	37.0	40.0	27.0	40.0
18	35.8934596832034	40.0	37.0	40.0	27.0	40.0
19	35.87238295917459	40.0	37.0	40.0	27.0	40.0
20	35.78723906939242	37.0	37.0	40.0	27.0	40.0
21	35.704678117221306	37.0	37.0	40.0	27.0	40.0
22	35.64099253245079	37.0	37.0	40.0	27.0	40.0
23	35.29933340521181	37.0	33.0	40.0	27.0	40.0
24	35.630998298355074	37.0	37.0	40.0	27.0	40.0
25	34.51447077905336	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.576519212405465
1101	2	0.6770687126075892
1101	3	0.4657751896082516
1101	4	0.6444673691497798
1101	5	0.5898559971911155
1101	6	0.5396603692350297
1101	7	0.5578478542435761
1101	8	0.6490281835331189
1101	9	0.8768739935710101
1101	10	0.9133309776252858
1101	11	0.6555518257821191
1101	12	0.6828507338153003
1101	13	0.7528461510755449
1101	14	1.3790656330165447
1101	15	1.1683720465503313
1101	16	1.0628534408042611
1101	17	1.159403710313967
1101	18	1.1325083500410003
1101	19	1.1626574104548837
1101	20	1.2274158454152015
1101	21	1.2249343885043444
1101	22	1.2212237960081822
1101	23	1.5266567694050863
1101	24	1.377607348541737
1101	25	1.2834235554371602
1102	1	0.30097865796894396
1102	2	0.5673014920689603
1102	3	0.33779015846681304
1102	4	0.36795190327833893
1102	5	0.22422307699343946
1102	6	0.29934220033936043
1102	7	0.34960094872845104
1102	8	0.4035892294175909
1102	9	0.6383663629614631
1102	10	0.6371173084604465
1102	11	0.43687091611748485
1102	12	0.4645479168712967
1102	13	0.51238216485077
1102	14	1.1735091701352403
1102	15	0.8435459607334508
1102	16	0.6739814483370807
1102	17	0.8245847822890937
1102	18	0.9321456616952943
1102	19	0.869544658092309
1102	20	0.9737764408628422
1102	21	0.9462398084651085
1102	22	0.9132504189619084
1102	23	1.280089747268356
1102	24	0.9540533334236727
1102	25	0.959591898640177
1103	1	0.39511254714285826
1103	2	0.6244543905501772
1103	3	0.28298278622915163
1103	4	0.12605301834261695
1103	5	0.39462979346233595
1103	6	0.2924174371613333
1103	7	0.296354271121686
1103	8	0.4521631101376755
1103	9	0.6232097037734903
1103	10	0.6357094514145345
1103	11	0.5073841545455764
1103	12	0.518088170005889
1103	13	0.5435417948192978
1103	14	1.1113639752255295
1103	15	0.9498748563372885
1103	16	0.7242498096435384
1103	17	0.6602170608971534
1103	18	0.822077605188376
1103	19	0.8945476398846068
1103	20	0.9872766431205662
1103	21	0.9063189231427771
1103	22	0.8554179504478441
1103	23	1.2923405288030807
1103	24	1.015998565826358
1103	25	0.9404987694723843
1104	1	0.21113005513225147
1104	2	0.40475881817209114
1104	3	0.23694713450000293
1104	4	0.25874183685419894
1104	5	0.2171238447488122
1104	6	0.13240325705037748
1104	7	0.11555406286765901
1104	8	0.32009277469433073
1104	9	0.5085648148270465
1104	10	0.4441629690369808
1104	11	0.3375843897470503
1104	12	0.2862216409543237
1104	13	0.24663394853484277
1104	14	0.8170688536284629
1104	15	0.605469917368012
1104	16	0.5742579854455201
1104	17	0.6308476179897582
1104	18	0.5402485540130897
1104	19	0.6461535636128772
1104	20	0.7168629737029093
1104	21	0.7143958306227916
1104	22	0.7800357480568465
1104	23	1.2100419903748474
1104	24	0.9673622241903317
1104	25	0.8926631331791484
1105	1	0.3213677330084721
1105	2	0.4091713848171601
1105	3	0.3451641066836224
1105	4	0.3544196094506802
1105	5	0.38993701693869554
1105	6	0.28181871957857396
1105	7	0.41407380232539026
1105	8	0.3999539980241167
1105	9	-1.466532847484764
1105	10	0.07262059962489786
1105	11	0.2611501068000308
1105	12	0.38191680423955177
1105	13	0.3851325729262314
1105	14	-1.456797404280067
1105	15	-0.11037833527817043
1105	16	5.201915677588431E-5
1105	17	0.2899304566631642
1105	18	0.30550462549241075
1105	19	0.4323655689337045
1105	20	0.5692712762947352
1105	21	0.6569731101752865
1105	22	0.7057519084184918
1105	23	1.0593563919684357
1105	24	0.8459939412156103
1105	25	0.4780924036064107
1106	1	0.09967210889169564
1106	2	0.13738764264874348
1106	3	0.14561414180831278
1106	4	-1.102254694794503
1106	5	-0.16273184706339805
1106	6	0.011958564866553445
1106	7	0.01706991818786463
1106	8	0.15079852224790358
1106	9	0.3147443639413794
1106	10	0.4708119842729559
1106	11	0.30878623540541383
1106	12	0.17662328842119024
1106	13	0.12657557494385685
1106	14	0.6221924136433259
1106	15	0.4421996027390023
1106	16	0.15288272222242938
1106	17	0.12710907593599785
1106	18	0.2686147128140277
1106	19	0.2732632192551421
1106	20	0.37109699451301736
1106	21	0.5427070222491963
1106	22	0.5483506557739304
1106	23	0.8810022901394987
1106	24	0.45360726940277374
1106	25	0.4156734762981884
1107	1	0.41290006814705293
1107	2	0.2977643549775095
1107	3	0.2534746089667266
1107	4	0.3392258110010715
1107	5	0.312162778858621
1107	6	0.15716755678787564
1107	7	0.18034968636489168
1107	8	0.3913631516823983
1107	9	0.49601148722904753
1107	10	0.43674717921499706
1107	11	0.13807056979248955
1107	12	0.25506111771517936
1107	13	0.19156986718130042
1107	14	0.6144588344371442
1107	15	0.5502673055841001
1107	16	0.4779749245406606
1107	17	0.470765117383138
1107	18	0.48488447045365035
1107	19	0.45993910615362665
1107	20	0.5802790282327237
1107	21	0.5504296249889222
1107	22	0.5284406627988716
1107	23	0.8220217843439244
1107	24	0.7662403041762147
1107	25	0.6704126754551964
1108	1	0.5572439943705589
1108	2	0.5145383020668426
1108	3	0.5877366026807742
1108	4	0.8155888957235078
1108	5	0.6282211921454532
1108	6	0.43816762712212665
1108	7	0.46382287453384663
1108	8	0.5658331968756016
1108	9	0.2984514514275318
1108	10	0.49115491653449084
1108	11	0.41922840951038154
1108	12	0.3941839254585702
1108	13	0.5098289978775128
1108	14	-0.9013140217817153
1108	15	0.13902278924241784
1108	16	0.4046353300796923
1108	17	0.4993196657338004
1108	18	0.7033110242545249
1108	19	0.6680184156091755
1108	20	0.7620616016113431
1108	21	0.789915191986438
1108	22	0.8608141128594795
1108	23	1.0760462965207154
1108	24	0.9979655758250274
1108	25	0.8899491037714924
1109	1	0.41898967967721035
1109	2	0.3485944531140923
1109	3	0.46637728494638253
1109	4	0.6900672083871981
1109	5	0.6393149028713054
1109	6	0.44737283104775116
1109	7	0.2933944100530965
1109	8	0.4285978723884796
1109	9	0.6730102916425977
1109	10	0.549780466858877
1109	11	0.39159664698305363
1109	12	0.3541841263250447
1109	13	0.3068769030844649
1109	14	0.7626974373793303
1109	15	0.5822426770503526
1109	16	0.4972765324266035
1109	17	0.555477725211631
1109	18	0.7127483103928043
1109	19	0.5797192769282447
1109	20	0.6830795458642029
1109	21	0.6687518095469827
1109	22	0.8127540487161227
1109	23	1.1075414814255424
1109	24	0.8631021496473537
1109	25	0.6729327364109565
1110	1	0.40497009459080147
1110	2	0.32559058715053624
1110	3	0.44568671922106873
1110	4	0.6489297042994977
1110	5	0.6187606527191747
1110	6	0.40230810865217137
1110	7	0.39808885685132367
1110	8	0.3592453419817758
1110	9	0.48390758962096925
1110	10	0.46961893544497
1110	11	0.3567016495149744
1110	12	0.2781239463681686
1110	13	0.2450275608849708
1110	14	0.6573453579560535
1110	15	0.5224602244232699
1110	16	0.4914141675504382
1110	17	0.5093582856433656
1110	18	0.6062602457699882
1110	19	0.5671916968263346
1110	20	0.6260847478131453
1110	21	0.6973035050688381
1110	22	0.7082063659218178
1110	23	0.9546931552641453
1110	24	0.8577252281411774
1110	25	0.7663728758270381
1111	1	0.26437436311720575
1111	2	0.17748388473926013
1111	3	0.38530123193646304
1111	4	0.6275855550392251
1111	5	0.41510980724905977
1111	6	0.3299000374885068
1111	7	0.2065125017599243
1111	8	0.3164569273842801
1111	9	0.35931701041773323
1111	10	0.228944352677388
1111	11	0.19815998837321303
1111	12	0.20500365135632848
1111	13	0.17062323711842708
1111	14	-1.516535111910386
1111	15	-0.3867219071499406
1111	16	0.09109750157436025
1111	17	0.25001394572447566
1111	18	0.33211127035492893
1111	19	0.32376790735310834
1111	20	0.3930133880865938
1111	21	0.5078669628375181
1111	22	0.2486729102595291
1111	23	-1.7017608452663708
1111	24	-0.1893936288319722
1111	25	0.10842373731573218
1112	1	-0.10777149931429975
1112	2	-0.24417913696441573
1112	3	0.09365895930198676
1112	4	0.2223032934547149
1112	5	-0.6558277212583832
1112	6	-0.7172690548947358
1112	7	-0.8048532401870077
1112	8	-0.6308358623566193
1112	9	-0.5338314513911087
1112	10	-0.7048226609773209
1112	11	-0.7354756212611449
1112	12	-0.7260199581388278
1112	13	-0.782178660549576
1112	14	-0.5310101352549026
1112	15	-0.8943291524603367
1112	16	-0.7261240274164678
1112	17	-0.8096803250131828
1112	18	-0.9611293113391426
1112	19	-0.7717372015791426
1112	20	-0.8329703688380192
1112	21	-1.7516835961328425
1112	22	-1.6297067052964564
1112	23	-1.3039697175929916
1112	24	-1.590322331809233
1112	25	-1.7668723539128237
1113	1	0.03533245434578447
1113	2	-0.06110459344872865
1113	3	-0.0946355361948008
1113	4	0.28734651476250406
1113	5	0.056641842013533505
1113	6	-0.011940619527926799
1113	7	-0.016016745744529715
1113	8	0.04584808998058065
1113	9	0.21831587603155356
1113	10	-0.026643835595869803
1113	11	-0.12628232834457265
1113	12	-0.050612656144977564
1113	13	-0.2624237118060577
1113	14	-0.033633076637407555
1113	15	-0.1591893685978789
1113	16	-0.023071261004950827
1113	17	-0.008085250982581726
1113	18	0.018319878795324485
1113	19	-0.10010892009935901
1113	20	-0.6655961919249762
1113	21	-0.4881115199144048
1113	22	-0.026304614181782426
1113	23	-0.24302142070370536
1113	24	-0.005721128743680026
1113	25	-0.9484526979111223
1114	1	-0.8648856171448465
1114	2	-0.7140397089620762
1114	3	-0.5345053204473302
1114	4	-1.4506081944288098
1114	5	-0.7710655354372449
1114	6	-0.5449334815159403
1114	7	-0.5931949242806596
1114	8	-0.34366098183151195
1114	9	-0.40768594160648775
1114	10	-0.47262216281322367
1114	11	-0.5131997369440313
1114	12	-0.5748899829930778
1114	13	-0.6214645570602002
1114	14	-0.583888098893155
1114	15	-1.3317978960006016
1114	16	-0.8067824568245001
1114	17	-0.9035677999205376
1114	18	-0.8542548666049825
1114	19	-0.8534207822644362
1114	20	-2.0650933941853324
1114	21	-6.735988283760218
1114	22	-7.720848381711246
1114	23	-8.012399725780519
1114	24	-5.959245774004483
1114	25	-7.222163989706683
1115	1	-1.1088768168309286
1115	2	-1.137802202671132
1115	3	-0.8858305563623396
1115	4	-0.651878958329462
1115	5	-0.6737516826416226
1115	6	-0.9458041990296735
1115	7	-1.1043833218988368
1115	8	-0.7947847095350369
1115	9	-2.8371091105200463
1115	10	-1.6788963504083938
1115	11	-1.248609104779426
1115	12	-1.2068663696145947
1115	13	-1.529713562399003
1115	14	-1.3172587019731452
1115	15	-2.170232884221207
1115	16	-1.5494265607419067
1115	17	-3.2973929901202865
1115	18	-2.9359304312796937
1115	19	-2.261315926247704
1115	20	-1.9116386967665946
1115	21	-1.492969586470835
1115	22	-1.0784712976243256
1115	23	-0.3848583988934209
1115	24	-1.4481896109331203
1115	25	-1.1347468794337487
1116	1	-1.652203117192542
1116	2	-0.9014603462112305
1116	3	-0.6784224539864319
1116	4	-0.37425651049238695
1116	5	-0.3923395755445682
1116	6	-0.5754549963245736
1116	7	-0.7351298595032816
1116	8	-1.837562266052963
1116	9	-0.9111248092312465
1116	10	-2.4631591700121973
1116	11	-1.139588723767723
1116	12	-0.8451852362276995
1116	13	-0.9307947053830361
1116	14	-1.1466238970833373
1116	15	-1.39181894143654
1116	16	-1.0146173831372138
1116	17	-0.7945981937039193
1116	18	-1.3446929127658862
1116	19	-0.9666748248835262
1116	20	-1.3006618574531714
1116	21	-0.7399845610955467
1116	22	-0.4476227400376729
1116	23	-0.18288880837528154
1116	24	-0.24715419548261508
1116	25	-0.21516349194290285
1201	1	0.24381950200539038
1201	2	0.4449646951203903
1201	3	0.2505425416547098
1201	4	0.2779421730183458
1201	5	0.3060492754984523
1201	6	0.2097461237724616
1201	7	0.2835569453231912
1201	8	0.3455935672647428
1201	9	0.697693113195271
1201	10	0.6259394067717778
1201	11	0.44157230209795983
1201	12	0.46142853892520463
1201	13	0.47188765101960684
1201	14	1.1737021057256243
1201	15	0.8868528092237682
1201	16	0.8445416147705487
1201	17	0.7839813184718025
1201	18	0.6825659685689658
1201	19	0.6558556373503635
1201	20	0.8090049270357511
1201	21	0.841535973901486
1201	22	0.8478956611375494
1201	23	0.8674456361201237
1201	24	0.7743440001824382
1201	25	0.794371504489547
1202	1	-0.26022110929134
1202	2	0.022037338036035692
1202	3	-0.09464954902652423
1202	4	-0.1856516285830807
1202	5	-0.10493577020726974
1202	6	-0.20964885295496316
1202	7	-0.20138258231424544
1202	8	-0.06447813973241523
1202	9	0.05434401581256765
1202	10	0.23806109721397206
1202	11	0.007239740125235983
1202	12	-0.013020716399054777
1202	13	0.06424419983507335
1202	14	0.23022364481424518
1202	15	0.23470269907695496
1202	16	0.2627441401778441
1202	17	0.11589405618638438
1202	18	0.07863847929993284
1202	19	0.06032094503675722
1202	20	0.24171902370227372
1202	21	0.23792569065651037
1202	22	0.2852937333664869
1202	23	0.531033253519027
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2211	3	0.40251158045418123
2211	4	0.5757650975707236
2211	5	0.3982790074053639
2211	6	0.3798072399397583
2211	7	0.2786200605565696
2211	8	0.4429799241674104
2211	9	0.6325656921296456
2211	10	0.5972719964393391
2211	11	0.3189179127176658
2211	12	0.3372799159868478
2211	13	0.27669318487192385
2211	14	0.5997623337189637
2211	15	0.392679089368805
2211	16	0.38424630111887836
2211	17	0.5025663002775644
2211	18	0.517218693274522
2211	19	0.47955962192587975
2211	20	0.4823423130194797
2211	21	0.45969555304811394
2211	22	0.6169625929411424
2211	23	0.6671784627116253
2211	24	0.6494786914041413
2211	25	0.712419380220382
2212	1	-0.3266621726520391
2212	2	-0.39133659140578203
2212	3	-0.33461333524381587
2212	4	-0.6016071772565468
2212	5	-0.2657156606003781
2212	6	-0.7145687030678047
2212	7	-0.5533687865016432
2212	8	-0.36700565263199536
2212	9	-0.28725105828766573
2212	10	-0.6413570273455704
2212	11	-0.5595157499347039
2212	12	-0.7993155284010882
2212	13	-0.6692855702661049
2212	14	-0.5887973350612441
2212	15	-0.8207981831306981
2212	16	-0.598429673759675
2212	17	-0.5973752362966991
2212	18	-0.5865302138668795
2212	19	-0.6340805670158787
2212	20	-0.6464431027867477
2212	21	-0.5534769964710691
2212	22	-0.6467838396089505
2212	23	-0.13982642017632685
2212	24	-0.43265870459816824
2212	25	-1.0529434644052174
2213	1	-0.16698816438999486
2213	2	-1.3252624243042064
2213	3	-0.27616241560250643
2213	4	0.09670220668698448
2213	5	0.03215726322604695
2213	6	-0.0168097139986898
2213	7	0.06014042124070329
2213	8	0.12329404687842072
2213	9	0.11077855611942766
2213	10	-0.4191631436835408
2213	11	-0.12497176964536294
2213	12	-0.012733723523787432
2213	13	-0.35152244186363646
2213	14	-0.023782817317702154
2213	15	-0.0986812769552543
2213	16	-0.11854377516161207
2213	17	0.026495865959475395
2213	18	0.05850535041210492
2213	19	0.08094476030954922
2213	20	0.04299804533687279
2213	21	-0.37747842587381086
2213	22	-0.8793233996922325
2213	23	-0.21782243698727655
2213	24	0.03441030963492153
2213	25	-0.6920217172970595
2214	1	-0.16443813216465486
2214	2	-0.42327965911137255
2214	3	-0.3667116436836402
2214	4	-0.5008829627341527
2214	5	-0.28420463575175603
2214	6	-0.3018836807122156
2214	7	-0.5066366895756289
2214	8	-0.15678438885296941
2214	9	-0.06601457273072953
2214	10	-2.02020894589468
2214	11	-0.912212152434293
2214	12	-0.5791977617860908
2214	13	-0.374990610866341
2214	14	-0.9402845554953174
2214	15	-0.7314294503436471
2214	16	-0.8746365399525189
2214	17	-0.6566221504224714
2214	18	-0.6001819595754583
2214	19	-1.496148569056352
2214	20	-2.6301598449549033
2214	21	-1.9611477681699654
2214	22	-2.1425538115152705
2214	23	-2.6844526391627355
2214	24	-1.6940766459832375
2214	25	-2.0628113372914143
2215	1	-0.34909096013532803
2215	2	-0.46233625983498783
2215	3	-0.8339199645628135
2215	4	-0.34437397456157015
2215	5	-0.17122133896641856
2215	6	-0.22366709166919208
2215	7	-0.24763762818383128
2215	8	-0.2314033664600359
2215	9	-0.2620233475424456
2215	10	-0.386648112453706
2215	11	-0.6891286246863757
2215	12	-0.5038934624935578
2215	13	-0.34074009273096095
2215	14	-0.22740388296630698
2215	15	-0.6535457371790798
2215	16	-0.5535307948351615
2215	17	-0.41707432578279935
2215	18	-0.380615069409977
2215	19	-2.333377353420282
2215	20	-2.5087387657994427
2215	21	-3.2020912318063424
2215	22	-2.404716952426682
2215	23	-3.658159934996746
2215	24	-2.589461607806541
2215	25	-1.8538681119366913
2216	1	-1.431487198332519
2216	2	-1.2154098556609263
2216	3	-0.8727561837755005
2216	4	-0.8571371517560706
2216	5	-1.8982014991152028
2216	6	-1.5564686468669464
2216	7	-1.1053887337079047
2216	8	-5.382023160801975
2216	9	-4.243546711109332
2216	10	-2.0392786434900856
2216	11	-1.1249131196681788
2216	12	-1.1758205405692905
2216	13	-2.0357173594712847
2216	14	-3.479772921562372
2216	15	-2.01885288854929
2216	16	-3.052053695315408
2216	17	-2.719723828313235
2216	18	-2.753302382936738
2216	19	-1.835524878211963
2216	20	-1.0078021447182763
2216	21	-0.7550237079739262
2216	22	-0.7506257185954439
2216	23	-1.0925550771044854
2216	24	-0.7549259781137394
2216	25	-0.4224701498525363
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	21.0
7	185.0
8	805.0
9	1687.0
10	2802.0
11	4709.0
12	7597.0
13	10976.0
14	15043.0
15	20539.0
16	25319.0
17	27093.0
18	28103.0
19	28288.0
20	28958.0
21	30970.0
22	32290.0
23	33984.0
24	35448.0
25	38037.0
26	40246.0
27	45078.0
28	50664.0
29	59650.0
30	70689.0
31	86083.0
32	114696.0
33	161344.0
34	235102.0
35	371074.0
36	644449.0
37	2014531.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.303248350924875	19.08941323546031	11.294130666490352	24.313207747124462
2	13.379981227067155	22.103557761463726	37.339757264665536	27.176703746803582
3	21.055600346879434	28.10494720497841	26.87764669640477	23.961805751737387
4	13.291277832638896	18.62089467697118	33.70479467406401	34.38303281632592
5	12.289379779896183	39.770119523780494	34.55918279307709	13.381317903246225
6	33.147791311523065	33.67396645006423	17.747053362963797	15.431188875448912
7	25.32551990247353	33.4510535872878	22.359105451079838	18.864321059158822
8	26.763652762711438	36.01859309061963	20.97849760777214	16.23925653889679
9	26.60903644392773	17.639957996193406	20.131988523959983	35.61901703591888
10	16.268271965032586	31.484666597286875	31.77781965566997	20.46924178201057
11	30.411858255037334	27.435785950433562	19.593450676742307	22.558905117786797
12	23.143219722398282	30.416951069040394	28.58928296848572	17.85054624007561
13	28.542045844755066	22.72150348482487	23.233861223456557	25.50258944696351
14	21.33283393039798	25.613510880764895	25.72765789086327	27.325997297973853
15	29.63841843929075	25.990819357355015	21.43898510395728	22.931777099396957
16	20.203738367702258	29.381598432760153	29.352229093038222	21.062434106499367
17	24.67158687674168	29.752840191929913	27.136504772581898	18.439068158746508
18	25.09753633015881	26.907299643560233	28.071125753833652	19.9240382724473
19	22.90291202970158	30.530981333383334	28.58659678795436	17.979509848960724
20	23.676611427716917	27.674329428276636	27.00205839349677	21.647000750509676
21	20.36635678710831	29.71317940930915	28.994714555252965	20.925749248329577
22	21.769990638010988	28.94745299242338	23.778021164991745	25.504535204573887
23	19.02904153754091	29.873353346017783	27.251256930918704	23.846348185522608
24	21.149809254239862	33.40626737408389	24.870406327377665	20.57351704429858
25	22.583477373598353	26.676860104695756	23.132236129081065	27.607426392624824
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	10532.0
1	10532.0
2	12883.0
3	15234.0
4	15234.0
5	15234.0
6	18333.0
7	21432.0
8	21432.0
9	21432.0
10	22363.5
11	23295.0
12	23295.0
13	23295.0
14	28291.5
15	33288.0
16	33288.0
17	33288.0
18	45624.0
19	57960.0
20	57960.0
21	57960.0
22	79024.5
23	100089.0
24	100089.0
25	100089.0
26	128579.0
27	157069.0
28	157069.0
29	157069.0
30	189954.5
31	222840.0
32	222840.0
33	222840.0
34	253988.0
35	285136.0
36	285136.0
37	285136.0
38	317826.5
39	350517.0
40	350517.0
41	350517.0
42	402604.5
43	454692.0
44	454692.0
45	454692.0
46	696761.0
47	938830.0
48	938830.0
49	938830.0
50	822745.5
51	706661.0
52	706661.0
53	706661.0
54	532956.0
55	359251.0
56	359251.0
57	359251.0
58	289315.0
59	219379.0
60	219379.0
61	219379.0
62	185975.0
63	152571.0
64	152571.0
65	152571.0
66	119726.0
67	86881.0
68	86881.0
69	86881.0
70	64921.5
71	42962.0
72	42962.0
73	42962.0
74	30651.5
75	18341.0
76	18341.0
77	18341.0
78	12326.0
79	6311.0
80	6311.0
81	6311.0
82	4275.0
83	2239.0
84	2239.0
85	2239.0
86	1445.5
87	652.0
88	652.0
89	652.0
90	442.0
91	232.0
92	232.0
93	232.0
94	140.5
95	49.0
96	49.0
97	49.0
98	33.0
99	17.0
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03145464858454081
2	0.017438344669819944
3	0.02346207394420667
4	0.026813798793379053
5	0.02812636237067733
6	0.01359440847916071
7	0.003070461225465608
8	0.0033751634844812798
9	0.004218954355601599
10	0.0020625999071830045
11	0.0015235112950783554
12	0.004781481603015145
13	0.002461056707434266
14	7.500363298847287E-4
15	0.0021563544484185953
16	0.002484495342743164
17	0.0026016885192876532
18	0.002367302166198675
19	4.6877270617795547E-4
20	6.094045180313421E-4
21	0.08487129845351885
22	0.08149613496903756
23	0.08196490767521551
24	0.0011250544948270933
25	0.23736305977320776
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4266460.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.23548431619946
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.43015104195926	43.258196795246555
2	10.061114335047627	10.309721314336446
3	2.560974925590056	3.936383719027482
4	0.9657140788453656	1.97915314162459
5	0.505080885611209	1.293903189657262
6	0.2818839898172602	0.866547764356198
7	0.19555130048610805	0.7013415912347879
8	0.13801500123860558	0.5657012345088661
9	0.10669399906331074	0.49198668440767723
>10	0.6583591483287881	6.477571398135456
>50	0.058159479739945785	2.05685338514201
>100	0.030721269351338423	2.898616770476534
>500	0.0027438782258579313	0.9818238687294147
>1k	0.0030694230950768647	3.4081833530433863
>5k	6.045833369090794E-4	2.5622493415513894
>10k+	0.0011626602632866913	18.21176644852192
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	130045	3.048077328745611	No Hit
CCCATGTACTCTGCGTTGATACCAC	128967	3.0228104798826196	No Hit
GAGTACATGGGAAGCAGTGGTATCA	60742	1.4237095859330686	No Hit
CATGTACTCTGCGTTGATACCACTG	50679	1.1878465988196303	No Hit
GCGTTGATACCACTGCTTCCCATGT	42166	0.9883134964349836	No Hit
GTATCAACGCAGAGTACATGGGAAG	33877	0.7940306483595299	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	33124	0.7763813559719299	No Hit
GCTTCCCATGTACTCTGCGTTGATA	30866	0.7234569174444387	No Hit
ACTCTGCGTTGATACCACTGCTTCC	27588	0.6466250709018718	No Hit
TATCAACGCAGAGTACATGGGAAGC	24837	0.582145385167094	No Hit
GGTATCAACGCAGAGTACATGGGAA	21009	0.4924222892046333	No Hit
CAGTGGTATCAACGCAGAGTACATG	18030	0.42259859461942684	No Hit
GTGGTATCAACGCAGAGTACATGGG	16792	0.3935815641070114	No Hit
GCAGTGGTATCAACGCAGAGTACAT	14759	0.34593081852402224	No Hit
ATACCACTGCTTCCCATGTACTCTG	14373	0.3368835052947877	No Hit
GTACATGGTAAGCAGTGGTATCAAC	13808	0.3236406763452605	No Hit
GTACTCTGCGTTGATACCACTGCTT	12959	0.30374127496800624	No Hit
CATGGGAAGCAGTGGTATCAACGCA	12509	0.2931938890790023	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	11970	0.2805604646475064	No Hit
ACATGGGAAGCAGTGGTATCAACGC	11943	0.2799276214941661	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	11334	0.26565349259104737	No Hit
TACCACTGCTTCCCATGTACTCTGC	10960	0.25688744298551963	No Hit
ACCATGTACTCTGCGTTGATACCAC	10531	0.24683226843800246	No Hit
CTTCCCATGTACTCTGCGTTGATAC	10396	0.24366805267130126	No Hit
CCATGTACTCTGCGTTGATACCACT	10044	0.23541765304256923	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	9941	0.23300347360575277	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	9902	0.23208936682870576	No Hit
GATACCACTGCTTCCCATGTACTCT	9901	0.2320659281933969	No Hit
GTACATGGGTAAGCAGTGGTATCAA	9851	0.23089399642795197	No Hit
GTTGATACCACTGCTTCCCATGTAC	9849	0.23084711915733416	No Hit
CCACTGCTTCCCATGTACTCTGCGT	9671	0.2266750420723504	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	8059	0.18889196195440716	No Hit
TTCCCATGTACTCTGCGTTGATACC	7732	0.18122752820839758	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	7688	0.1801962282548061	No Hit
TGATACCACTGCTTCCCATGTACTC	7152	0.1676331197292369	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	6718	0.15746075200517526	No Hit
CCCCATGTACTCTGCGTTGATACCA	5946	0.13936612554670616	No Hit
ATCAACGCAGAGTACATGGGAAGCA	5122	0.1200526900521744	No Hit
GCTTACCATGTACTCTGCGTTGATA	4985	0.1168415970148554	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	4981	0.11674784247361983	No Hit
GAGTACATGGTAAGCAGTGGTATCA	4904	0.1149430675548347	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.3438635308897776E-5	0.0
4	0.0	0.0	0.0	2.3438635308897776E-5	0.0
5	0.0	0.0	0.0	2.3438635308897776E-5	0.0
6	0.0	0.0	0.0	2.3438635308897776E-5	0.0
7	0.0	0.0	0.0	2.3438635308897776E-5	0.0
8	0.0	0.0	0.0	4.687727061779555E-5	0.0
9	0.0	0.0	0.0	7.031590592669333E-5	0.0
10	0.0	0.0	0.0	1.4063181185338666E-4	0.0
11	0.0	0.0	0.0	1.4063181185338666E-4	0.0
12	0.0	0.0	0.0	1.875090824711822E-4	0.0
13	0.0	0.0	0.0	1.875090824711822E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACAAA	925	0.0	12.428086	1
TACGACC	115	5.347829E-9	12.389164	4
GTCGTAC	130	2.6175258E-9	11.693304	1
CGACCAT	115	7.1288014E-8	11.560102	6
GTCTTAG	275	0.0	11.400971	1
TCGTACC	100	1.9335512E-6	11.399233	2
ACGCACG	60	0.0058999546	11.077912	11
GTACGAC	160	5.456968E-10	10.68628	3
GTATCGA	155	3.6307028E-9	10.426111	18
GGTATCG	165	9.2040864E-10	10.370356	17
GTCTAGC	150	2.4456313E-8	10.134196	1
AGCGTTC	75	0.0026591904	10.128732	8
TGGTCGA	75	0.0026597076	10.128495	10
ATACGAC	160	5.9462764E-9	10.1178665	19
TAGACAG	460	0.0	10.117699	5
GAACAAA	1745	0.0	10.072492	1
ACCGCTC	125	2.5879035E-6	9.875514	8
ACACCGT	145	1.6299236E-7	9.823239	6
TATCGAC	180	4.0217856E-9	9.522697	19
CGTACCC	120	1.7043487E-5	9.498916	3
>>END_MODULE