FastQCFastQC Report
Wed 25 May 2016
SRR1294774_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294774_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4266460
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1424833.3396070747176814No Hit
GTACATGGGAAGCAGTGGTATCAAC1267352.97049544587316No Hit
GAGTACATGGGAAGCAGTGGTATCA655761.5370119490162804No Hit
CATGTACTCTGCGTTGATACCACTG583681.3680662657097453No Hit
GCGTTGATACCACTGCTTCCCATGT452721.0611138977044199No Hit
ACGCAGAGTACATGGGAAGCAGTGG411610.9647576679495413No Hit
GTATCAACGCAGAGTACATGGGAAG359120.841728271213137No Hit
GCTTCCCATGTACTCTGCGTTGATA339170.7949681937718858No Hit
ACTCTGCGTTGATACCACTGCTTCC314840.7379419940653376No Hit
TATCAACGCAGAGTACATGGGAAGC292810.6863066804798358No Hit
GGTATCAACGCAGAGTACATGGGAA235130.5511126320181134No Hit
CAGTGGTATCAACGCAGAGTACATG224610.5264551876731529No Hit
GTGGTATCAACGCAGAGTACATGGG181620.4256924944802014No Hit
GCAGTGGTATCAACGCAGAGTACAT167080.391612718741064No Hit
CATGGGAAGCAGTGGTATCAACGCA163140.38237789642935827No Hit
ATACCACTGCTTCCCATGTACTCTG160650.3765416762374428No Hit
ACATGGGAAGCAGTGGTATCAACGC155240.3638613745353291No Hit
GTACATGGTAAGCAGTGGTATCAAC138490.3246016603929253No Hit
GGGAAGCAGTGGTATCAACGCAGAG138310.3241797649573651No Hit
CTGCTTCCCATGTACTCTGCGTTGA134510.315273083539984No Hit
GTACTCTGCGTTGATACCACTGCTT129230.30289748409688594No Hit
AAGCAGTGGTATCAACGCAGAGTAC123200.2887639870056206No Hit
TACCACTGCTTCCCATGTACTCTGC120820.28318559180210295No Hit
CCATGTACTCTGCGTTGATACCACT120120.28154488733048005No Hit
CTTCCCATGTACTCTGCGTTGATAC119490.2800682533060195No Hit
GCAGAGTACATGGGAAGCAGTGGTA116450.2729429081721146No Hit
GTATCAACGCAGAGTACTTTTTTTT116420.2728725922661879No Hit
ACCATGTACTCTGCGTTGATACCAC115080.2697318151347956No Hit
GATACCACTGCTTCCCATGTACTCT114920.26935679696985326No Hit
CCACTGCTTCCCATGTACTCTGCGT110780.25965320195196956No Hit
GTTGATACCACTGCTTCCCATGTAC106610.2498792910281592No Hit
GTACATGGGTAAGCAGTGGTATCAA105840.24807451610937406No Hit
ATGGGAAGCAGTGGTATCAACGCAG100020.23443323035959554No Hit
TATCAACGCAGAGTACTTTTTTTTT86500.20274419542196573No Hit
GGTATCAACGCAGAGTACTTTTTTT82110.19245463452135964No Hit
GGTAAGCAGTGGTATCAACGCAGAG81850.1918452300033283No Hit
TTCCCATGTACTCTGCGTTGATACC79980.1874622052005644No Hit
TGATACCACTGCTTCCCATGTACTC74240.17400842853325707No Hit
CCCCATGTACTCTGCGTTGATACCA67780.1588670701237091No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA64460.15108544320115505No Hit
ATCAACGCAGAGTACATGGGAAGCA64400.15094481138930166No Hit
ACGCAGAGTACTTTTTTTTTTTTTT61540.14424136169095692No Hit
GTACTTTTTTTTTTTTTTTTTTTTT55090.12912344191671785No Hit
GCTTACCATGTACTCTGCGTTGATA54630.12804526469250854No Hit
GAGTACATGGTAAGCAGTGGTATCA54350.12738898290385942No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTCGA751.4895653E-512.65874410
TCTACGC1103.7342033E-812.1107413
TGACCCG657.9188345E-411.7114855
GTCGTAC1156.89779E-811.5875881
AACGCGA600.005899250511.078096
CCGCGCG600.00590594411.0764019
CGCGCGC951.3711002E-510.9931210
GGGTACG1053.390809E-610.8781451
GTATCGC700.001498230410.8520056
CTAGTAC1002.3580324E-510.4671393
GGCGTGT951.6555736E-49.9938628
CGTACCC1054.0354476E-59.9687053
TAGACAG5650.09.93662555
GTATACG1151.0041551E-59.9322191
TAGGACC2300.09.9293024
CGGTACT1151.00766665E-59.9293024
AAAAGTA9600.09.9122972
CTACGCA1451.5812293E-79.8437054
CTAGGAC3300.09.8039323
TAGGACT4600.09.7224424
Produced by FastQC (version 0.11.5)