FastQCFastQC Report
Wed 25 May 2016
SRR1294773_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294773_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1957460
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC74960.3829452453689986No Hit
CCCATGTACTCTGCGTTGATACCAC66100.33768250692223595No Hit
GAGTACATGGGAAGCAGTGGTATCA34570.17660641852196213No Hit
GTATCAACGCAGAGTACTTTTTTTT30800.15734676570657893No Hit
CATGTACTCTGCGTTGATACCACTG24700.12618393223871752No Hit
GGTATCAACGCAGAGTACTTTTTTT22040.11259489338224025No Hit
GCGTTGATACCACTGCTTCCCATGT22010.11244163354551308No Hit
GTATCAACGCAGAGTACATGGGAAG20640.10544276766830485No Hit
ACGCAGAGTACATGGGAAGCAGTGG19870.10150909852564037No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGCG400.005295457314.2436219
GTGCTAG2350.011.7256441
TGGACCG658.032841E-411.6909565
AGCACCG855.3305375E-511.1751795
TACACCG855.3305375E-511.1751795
TATACCG600.005881641511.0820515
TTAGACA1502.1373125E-910.7656974
GTGTTAC1451.4393663E-810.4847611
GCACCGT1002.4090912E-510.4461236
GTATAAG2550.010.4333651
CTTAGAC1106.051301E-610.36296753
TATACTG6450.010.3088865
TAGACTG2650.010.0365755
ACACCGT1054.122463E-59.9486886
TCTATAC3150.09.650173
CCTAGTA900.0011134019.5003582
CTTATAC2700.09.4993873
CTAGTAC800.00451873669.4993873
ACGGGTC900.00111496349.4989015
CGGGTCT1002.7659468E-49.4964756
Produced by FastQC (version 0.11.5)