FastQCFastQC Report
Wed 25 May 2016
SRR1294773_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294773_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1957460
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT98670.504071602995719No Hit
GTACATGGGAAGCAGTGGTATCAAC78330.400161433694686No Hit
GGTATCAACGCAGAGTACTTTTTTT75620.3863169617769967No Hit
CCCATGTACTCTGCGTTGATACCAC69610.3556139078193169No Hit
TATCAACGCAGAGTACTTTTTTTTT65860.3364564282284185No Hit
GAGTACATGGGAAGCAGTGGTATCA40710.20797359843879315No Hit
ACGCAGAGTACTTTTTTTTTTTTTT40670.20776925198982354No Hit
CATGTACTCTGCGTTGATACCACTG27100.1384447191768925No Hit
ACGCAGAGTACATGGGAAGCAGTGG26480.13527734921786397No Hit
GTATCAACGCAGAGTACATGGGAAG24140.12332308195314337No Hit
GCGTTGATACCACTGCTTCCCATGT22840.11668182236163191No Hit
ATTCCATTCCATTCCATTCCATTCC21340.10901883052527254No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGCG307.754643E-418.9887114
ATTAACG400.00522738514.2714253
CGTGTTA400.00529971314.24189710
GATCGGT551.970043E-413.81032411
ATACCGT752.0843367E-411.3940996
TAGAACC951.3381119E-511.0165394
CGAATAG951.3691879E-510.99374510
TAGACTG3150.010.8734675
TATTCCG700.001473120910.87346655
CACCGTT700.00149879710.8509697
CCGAATA1053.4908153E-610.8509689
TCGGTGC803.7923877E-410.681149513
TAACCCG1002.3597904E-510.4657115
AGTACTG3300.010.0909065
ATCGGTG856.6307024E-410.05284712
ATTAGAC951.6195745E-410.0150363
ACACCGT951.6529298E-49.9948246
CCCGTCA951.6542085E-49.99405812
GTGTAGG3550.09.9201041
TATACTG6200.09.8211955
Produced by FastQC (version 0.11.5)