FastQCFastQC Report
Wed 25 May 2016
SRR1294772_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294772_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3489626
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC944982.7079692780830955No Hit
CCCATGTACTCTGCGTTGATACCAC907412.600307310869417No Hit
GAGTACATGGGAAGCAGTGGTATCA442331.2675570390637851No Hit
CATGTACTCTGCGTTGATACCACTG349521.0015973058430903No Hit
GCGTTGATACCACTGCTTCCCATGT313650.8988069208562752No Hit
GTATCAACGCAGAGTACATGGGAAG275160.7885085679668825No Hit
GCTTCCCATGTACTCTGCGTTGATA251550.7208508877455636No Hit
ACGCAGAGTACATGGGAAGCAGTGG239830.6872656267462472No Hit
ACTCTGCGTTGATACCACTGCTTCC194140.5563346903077865No Hit
TATCAACGCAGAGTACATGGGAAGC175460.5028045985443712No Hit
GGTATCAACGCAGAGTACATGGGAA150860.4323099380850555No Hit
CAGTGGTATCAACGCAGAGTACATG133300.38198935931816186No Hit
GTGGTATCAACGCAGAGTACATGGG128560.36840624181502546No Hit
GCAGTGGTATCAACGCAGAGTACAT110300.3160797174253058No Hit
ATACCACTGCTTCCCATGTACTCTG104710.3000608088087377No Hit
GTACTCTGCGTTGATACCACTGCTT96200.27567424130838086No Hit
CATGGGAAGCAGTGGTATCAACGCA93570.268137617039763No Hit
CTGCTTCCCATGTACTCTGCGTTGA91150.2612027764579929No Hit
GGGAAGCAGTGGTATCAACGCAGAG87740.251430955638226No Hit
GTACATGGTAAGCAGTGGTATCAAC85940.24627280975095897No Hit
ACATGGGAAGCAGTGGTATCAACGC85450.24486864781498074No Hit
TACCACTGCTTCCCATGTACTCTGC82470.2363290507349498No Hit
GATACCACTGCTTCCCATGTACTCT75330.21586840538212407No Hit
CCATGTACTCTGCGTTGATACCACT74340.21303142514412718No Hit
CTTCCCATGTACTCTGCGTTGATAC73780.21142666864586634No Hit
GCAGAGTACATGGGAAGCAGTGGTA73130.20956400485324214No Hit
CCACTGCTTCCCATGTACTCTGCGT72140.2067270246152453No Hit
GTTGATACCACTGCTTCCCATGTAC69850.20016471679200004No Hit
AAGCAGTGGTATCAACGCAGAGTAC69820.20007874769387896No Hit
GTACATGGGTAAGCAGTGGTATCAA60940.1746318946500284No Hit
ACCATGTACTCTGCGTTGATACCAC60080.17216744717055638No Hit
ATGGGAAGCAGTGGTATCAACGCAG58460.1675251158720161No Hit
TTCCCATGTACTCTGCGTTGATACC54700.15675032224083613No Hit
TGATACCACTGCTTCCCATGTACTC52150.14944294890054122No Hit
CCCCATGTACTCTGCGTTGATACCA44960.12883902171751357No Hit
ATCAACGCAGAGTACATGGGAAGCA44740.12820858166462537No Hit
GGTAAGCAGTGGTATCAACGCAGAG41300.11835079174673732No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA39100.11204639121785544No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGGT550.003078671912.086236
AGACCGT951.0458716E-611.9953576
AAGCGGT1251.8446372E-811.39575310
TCAGGCG600.005885048311.0815853
GGTATCG700.001485263810.86321617
AGCGGTG1252.2544009E-710.63603611
TATTCCG1355.7178113E-810.5526825
TAGACTG3850.010.3608155
TATCGAC750.00260271910.15487819
CTGTTCG750.002657816510.1292699
TTATACG856.60864E-410.056954
ACCGTGT1356.4864435E-79.8477588
GGAACGA1201.6934117E-59.5039515
CTCGGCG900.00111834199.4961887
GTTCTAG3150.09.3496081
CTAGGAC2750.09.3258023
ACTCCCG950.00181561919.00490518
GTCTAGC1901.0584699E-89.0003871
TCAGTAC1701.6908234E-78.9397663
TAGGACT3400.08.939514
Produced by FastQC (version 0.11.5)