FastQCFastQC Report
Wed 25 May 2016
SRR1294772_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294772_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3489626
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC989542.835662045158994No Hit
GTACATGGGAAGCAGTGGTATCAAC916692.6269004185548823No Hit
GAGTACATGGGAAGCAGTGGTATCA470321.347766207610787No Hit
CATGTACTCTGCGTTGATACCACTG396021.1348494079308211No Hit
GCGTTGATACCACTGCTTCCCATGT335850.9624240534659015No Hit
ACGCAGAGTACATGGGAAGCAGTGG290670.8329545916954997No Hit
GTATCAACGCAGAGTACATGGGAAG289340.8291432950121302No Hit
GCTTCCCATGTACTCTGCGTTGATA277230.7944404357372394No Hit
ACTCTGCGTTGATACCACTGCTTCC221480.6346811950621642No Hit
TATCAACGCAGAGTACATGGGAAGC198980.5702043714713267No Hit
GGTATCAACGCAGAGTACATGGGAA165060.47300197786238407No Hit
CAGTGGTATCAACGCAGAGTACATG160310.4593902039932073No Hit
GTGGTATCAACGCAGAGTACATGGG135800.389153450828255No Hit
GCAGTGGTATCAACGCAGAGTACAT122590.35129839128892326No Hit
CATGGGAAGCAGTGGTATCAACGCA121740.3488626001754916No Hit
ATACCACTGCTTCCCATGTACTCTG115420.33075177683797635No Hit
ACATGGGAAGCAGTGGTATCAACGC108410.31066366424367536No Hit
GGGAAGCAGTGGTATCAACGCAGAG102730.294386848332744No Hit
CTGCTTCCCATGTACTCTGCGTTGA101070.28962989157004215No Hit
GTACTCTGCGTTGATACCACTGCTT92520.2651286986055239No Hit
TACCACTGCTTCCCATGTACTCTGC89270.2558153796424029No Hit
CCATGTACTCTGCGTTGATACCACT88960.2549270322951514No Hit
GTATCAACGCAGAGTACTTTTTTTT88780.25441121770642466No Hit
GATACCACTGCTTCCCATGTACTCT86590.24813547354358317No Hit
CTTCCCATGTACTCTGCGTTGATAC85920.24621549701887824No Hit
AAGCAGTGGTATCAACGCAGAGTAC85810.24590027699243414No Hit
GCAGAGTACATGGGAAGCAGTGGTA82700.23698814715387836No Hit
CCACTGCTTCCCATGTACTCTGCGT81920.23475295060272935No Hit
GTACATGGTAAGCAGTGGTATCAAC81490.23352072686299336No Hit
ATGGGAAGCAGTGGTATCAACGCAG72210.2069276191775279No Hit
GTTGATACCACTGCTTCCCATGTAC71680.20540883177738817No Hit
ACCATGTACTCTGCGTTGATACCAC65920.18890276493813377No Hit
GTACATGGGTAAGCAGTGGTATCAA64360.18443237183583572No Hit
GGTATCAACGCAGAGTACTTTTTTT61990.17764081308426746No Hit
TATCAACGCAGAGTACTTTTTTTTT59740.17119313072518375No Hit
TTCCCATGTACTCTGCGTTGATACC56010.16050430619212488No Hit
TGATACCACTGCTTCCCATGTACTC54020.1548016893500908No Hit
CCCCATGTACTCTGCGTTGATACCA53010.15190739638001322No Hit
ATCAACGCAGAGTACATGGGAAGCA52090.14927101070429896No Hit
GGTAAGCAGTGGTATCAACGCAGAG48330.13849621707311902No Hit
ACGCAGAGTACTTTTTTTTTTTTTT42040.12047136283372488No Hit
GTACTTTTTTTTTTTTTTTTTTTTT36480.1045384233152779No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCG402.7290103E-416.6514875
TAGACCG350.00214796416.3116635
TCGTAAG400.00522486714.2729082
TAGACTG3150.012.9889155
ACGTACT1501.70985E-1011.4181634
CGTACTC2050.011.139675
ATTAACG803.7181083E-410.7045283
TAACCCG1002.3589115E-510.466655
CTATGCG750.002662578410.1270839
TCTGTCG2300.09.9069298
CCACGTA1659.969881E-99.8036142
CTTAGAC2251.8189894E-129.72658353
TATACTG5400.09.5151365
GTCCAAG3150.09.3673311
TTAGACT3050.09.359154
GTACTAG1856.486516E-99.2611621
GCTATAC1856.526534E-99.257973
GTCTAAG1651.03556886E-79.22997951
CTAGGAC3650.09.1241033
ATTAGAC2201.6370905E-109.0826293
Produced by FastQC (version 0.11.5)