FastQCFastQC Report
Wed 25 May 2016
SRR1294771_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294771_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2405622
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC31690.13173308192226377No Hit
GTATCAACGCAGAGTACTTTTTTTT31330.13023658746054034No Hit
CCCATGTACTCTGCGTTGATACCAC28770.11959484906606274No Hit
GGTATCAACGCAGAGTACTTTTTTT24690.10263457849986407No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCTG855.341073E-511.1732545
GTGTAGG2650.010.7567661
GTCTAGA3450.010.7411761
GTCCAGG4700.010.7148071
TAGACTG3450.010.4607565
GTCTTAC2053.6379788E-1210.1970631
TAGGACT3050.09.9655884
GTCTCGC1252.5649333E-69.8818821
TCTAGAC2700.09.8506553
CTAGTAC1856.511982E-109.7555713
ACTGATC2650.09.674658
GTCTAAA2106.730261E-119.501811
GCCTAAG2002.6375346E-109.501811
CTAGACT3100.09.498454
CTACACT4000.09.498454
GGACAGT3000.09.496286
ACTGACC2900.09.4954898
CTAGGAC2650.09.3196233
CTAGACA2650.09.3192354
CCTAGTA2357.2759576E-129.29770852
Produced by FastQC (version 0.11.5)