##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294771_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2405622
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16961268229173	33.0	33.0	33.0	27.0	33.0
2	31.121806751019072	33.0	33.0	33.0	27.0	33.0
3	31.102798777197748	33.0	33.0	33.0	27.0	33.0
4	34.5612698088062	37.0	37.0	37.0	27.0	37.0
5	34.77729917667863	37.0	37.0	37.0	27.0	37.0
6	34.98464139420075	37.0	37.0	37.0	33.0	37.0
7	34.912761439660926	37.0	37.0	37.0	33.0	37.0
8	34.856338194446174	37.0	37.0	37.0	33.0	37.0
9	34.518706180771545	37.0	37.0	37.0	27.0	37.0
10	34.59388050159169	37.0	37.0	37.0	27.0	37.0
11	34.85201083129436	37.0	37.0	37.0	33.0	37.0
12	34.777045188313046	37.0	37.0	37.0	27.0	37.0
13	34.70658607212604	37.0	37.0	37.0	27.0	37.0
14	35.874743829246654	40.0	37.0	40.0	27.0	40.0
15	36.206340813311485	40.0	37.0	40.0	27.0	40.0
16	36.237129939782726	40.0	37.0	40.0	27.0	40.0
17	36.17131619182066	40.0	37.0	40.0	27.0	40.0
18	36.18327318256983	40.0	37.0	40.0	27.0	40.0
19	36.280598115580915	40.0	37.0	40.0	27.0	40.0
20	36.1771816187248	40.0	37.0	40.0	27.0	40.0
21	36.12600732783455	40.0	37.0	40.0	27.0	40.0
22	35.97938204755361	40.0	37.0	40.0	27.0	40.0
23	35.60561426525032	37.0	37.0	40.0	27.0	40.0
24	35.91132397359186	37.0	37.0	40.0	27.0	40.0
25	34.783865461822344	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5175204825950281
1101	2	0.6310629145769155
1101	3	0.41982594908306226
1101	4	0.5007173164746135
1101	5	0.44517704274576886
1101	6	0.30020449024839735
1101	7	0.4334817675077858
1101	8	0.5212104461932725
1101	9	0.6180185792205677
1101	10	0.7044694950509225
1101	11	0.4748139303458032
1101	12	0.42113106802754174
1101	13	0.4972048905570432
1101	14	1.0950742233619124
1101	15	0.9060820539507475
1101	16	0.733719749961601
1101	17	0.9119294667797107
1101	18	0.744212129076196
1101	19	0.837806867655992
1101	20	0.8870564184322589
1101	21	0.8826105438258196
1101	22	0.9199312539347275
1101	23	1.2065516688708513
1101	24	1.1097556784245697
1101	25	0.7812069206652055
1102	1	0.2747588623593984
1102	2	0.4895238031421769
1102	3	0.3794947276605889
1102	4	0.2008111035650657
1102	5	0.2747691004083208
1102	6	0.24090034962775064
1102	7	0.24173971956169282
1102	8	0.39366302775791695
1102	9	0.5282544281701718
1102	10	0.621072834637566
1102	11	0.38528771268262574
1102	12	0.36863247760642537
1102	13	0.3502593478130933
1102	14	0.9435829935008684
1102	15	0.8707547430542277
1102	16	0.5767542878579093
1102	17	0.7148036718515698
1102	18	0.648683386395362
1102	19	0.6498767948670263
1102	20	0.7507629316318898
1102	21	0.6977438633973279
1102	22	0.7691200497926758
1102	23	1.0457510431866979
1102	24	0.8023626293561037
1102	25	0.7384565417739282
1103	1	0.24690480749601562
1103	2	0.47786692082744864
1103	3	0.26312818811641847
1103	4	0.05887869757458475
1103	5	0.17815939511582712
1103	6	0.21063536224605883
1103	7	0.19339244860669424
1103	8	0.28771972195882967
1103	9	0.5124792867558057
1103	10	0.38293140049151475
1103	11	0.3143409600338458
1103	12	0.3922448671294916
1103	13	0.2842414784901237
1103	14	0.9576464216600229
1103	15	0.5355366297659714
1103	16	0.3436039781463194
1103	17	0.3095135749002864
1103	18	0.46676338558754793
1103	19	0.4842786143600364
1103	20	0.6797441713175161
1103	21	0.6859866669553725
1103	22	0.6281785354946408
1103	23	1.1649818324726482
1103	24	0.8231849287561985
1103	25	0.5587582671355378
1104	1	0.2355681274367356
1104	2	0.4700093903467213
1104	3	0.35439362436881794
1104	4	0.34364034553635037
1104	5	0.2808141793059633
1104	6	0.2261643084959033
1104	7	0.18090208351651427
1104	8	0.43451681217162985
1104	9	0.47344721896481445
1104	10	0.5634694561840803
1104	11	0.38709032507087215
1104	12	0.2743313422929532
1104	13	0.4332483577874626
1104	14	0.910363995331636
1104	15	0.7198349457994198
1104	16	0.6633335431306477
1104	17	0.6504136075511369
1104	18	0.5410216207192562
1104	19	0.5921901698056615
1104	20	0.6170098618620656
1104	21	0.6991093697028745
1104	22	0.7695002666005664
1104	23	1.1764379038269652
1104	24	0.8484514548782514
1104	25	0.5825470230604708
1105	1	0.19838227292274624
1105	2	0.32526143898210336
1105	3	0.38115889162913064
1105	4	0.3032921869144616
1105	5	0.25101319698144664
1105	6	0.14453617317673917
1105	7	0.1656246912378947
1105	8	0.32561172505204894
1105	9	-1.7229127324598679
1105	10	-0.2588889784325943
1105	11	0.0825813492779588
1105	12	0.10136565971078682
1105	13	0.17511787774533616
1105	14	-1.4747323891464106
1105	15	-0.13529664948572417
1105	16	-0.07491083152835643
1105	17	0.1575511951482511
1105	18	0.07047698589069995
1105	19	0.2621705145961073
1105	20	0.265350423203742
1105	21	0.4267117070145545
1105	22	0.5463923247365798
1105	23	0.9125782200315413
1105	24	0.7793204353795034
1105	25	0.30175718493136827
1106	1	0.12600223824086498
1106	2	0.1273603763603468
1106	3	0.10327662003256322
1106	4	-1.183142209560252
1106	5	-0.3532274658422523
1106	6	-0.14067958341940567
1106	7	0.11606393063981102
1106	8	0.1975460655856338
1106	9	0.4311771653505332
1106	10	0.27232170952320445
1106	11	0.21408263884697476
1106	12	0.09363855696957302
1106	13	0.11016632244375302
1106	14	0.4810395262601972
1106	15	0.3271570245153228
1106	16	0.20273038167816537
1106	17	0.009430354501567706
1106	18	0.10482833421820459
1106	19	0.25511308164306
1106	20	0.3175983880912199
1106	21	0.5309680398514871
1106	22	0.5557884056743916
1106	23	0.7429187432216153
1106	24	0.4653364019051054
1106	25	0.4376020722607237
1107	1	0.3007453504229929
1107	2	0.34604150685958857
1107	3	0.35789639674978346
1107	4	0.28766329313454975
1107	5	0.2925251782451852
1107	6	0.12476473357642703
1107	7	0.2548612613669192
1107	8	0.12411618413856473
1107	9	0.40447556559182374
1107	10	0.48897681839149243
1107	11	0.22022781353685872
1107	12	0.26958983834243355
1107	13	0.2196198944205534
1107	14	0.749840683017112
1107	15	0.4389640676499482
1107	16	0.5048000632460372
1107	17	0.5605618488207682
1107	18	0.5472714625221116
1107	19	0.49239461804086915
1107	20	0.5959032948973118
1107	21	0.6243551793002453
1107	22	0.5753921801762161
1107	23	0.8334758933848008
1107	24	0.5506009417825979
1107	25	0.5504805580168579
1108	1	0.47198479125729165
1108	2	0.3767853639883185
1108	3	0.4288413628137775
1108	4	0.610056186920346
1108	5	0.46558052538573946
1108	6	0.3432083642935524
1108	7	0.3717635727944355
1108	8	0.49101169476306694
1108	9	0.21897723031811722
1108	10	0.5573388578530611
1108	11	0.3478066504672057
1108	12	0.3702079945748977
1108	13	0.325421181573617
1108	14	-1.0382324178954079
1108	15	0.02229311218859209
1108	16	0.2939823383682949
1108	17	0.49267586556985776
1108	18	0.5932131844192625
1108	19	0.4846460220390796
1108	20	0.6962488675929279
1108	21	0.6769709703237012
1108	22	0.727333699152112
1108	23	1.0084268265101173
1108	24	0.9297466285846667
1108	25	0.7825361797628148
1109	1	0.47033410536622355
1109	2	0.3120564206356953
1109	3	0.44692169566314277
1109	4	0.5801725562594839
1109	5	0.49853656942448765
1109	6	0.3221138707576472
1109	7	0.23417035401556774
1109	8	0.3070407947683975
1109	9	0.4611534958704979
1109	10	0.4401935639179442
1109	11	0.28928122074324847
1109	12	0.36470937469331943
1109	13	0.3875770926949116
1109	14	0.6204718656818784
1109	15	0.5866996757400358
1109	16	0.5307052836475279
1109	17	0.5779468787990822
1109	18	0.6336066136893521
1109	19	0.48506493487163027
1109	20	0.5666077562734131
1109	21	0.5695603162031446
1109	22	0.723970022317495
1109	23	1.104894151743359
1109	24	0.8578337172989521
1109	25	0.8073702789924013
1110	1	0.4078919980120155
1110	2	0.18671680253864054
1110	3	0.3833806128469739
1110	4	0.45025407304399323
1110	5	0.565336874546162
1110	6	0.4180159577629112
1110	7	0.2139289606753465
1110	8	0.3386350555320661
1110	9	0.4116071688106757
1110	10	0.5043538799794334
1110	11	0.252932165933899
1110	12	0.14919833808996685
1110	13	0.2657486670822493
1110	14	0.6921804542211873
1110	15	0.47456499732770396
1110	16	0.4405668562115608
1110	17	0.37376866174062684
1110	18	0.427415448937154
1110	19	0.3767020713649032
1110	20	0.3968670815617088
1110	21	0.5291461143703842
1110	22	0.5703183680650383
1110	23	0.9015917807757248
1110	24	0.6141069447007013
1110	25	0.5478661073454205
1111	1	0.1926018573619821
1111	2	0.07475440171545245
1111	3	0.32965840013001113
1111	4	0.48769780954770425
1111	5	0.337569097405364
1111	6	0.30460714829708024
1111	7	0.2757734468509838
1111	8	0.3344818200737194
1111	9	0.3173513779177526
1111	10	0.2396730977602033
1111	11	0.13485325094313083
1111	12	0.03746695991162596
1111	13	0.1332395949609122
1111	14	-1.630548043208222
1111	15	-0.43879426984915426
1111	16	0.03743592698952369
1111	17	0.11437174669978845
1111	18	0.29504574898453484
1111	19	0.26928171366323284
1111	20	0.2862375089942901
1111	21	0.43292001064590835
1111	22	0.2515449093532993
1111	23	-1.7581413791044298
1111	24	-0.4638364406830675
1111	25	0.006591153365476998
1112	1	-0.1422389617327049
1112	2	-0.19200095238719328
1112	3	0.0569730268351627
1112	4	0.3060966401577119
1112	5	-0.6607368120471762
1112	6	-0.8386515531543282
1112	7	-0.9845649097628026
1112	8	-0.6948448275913606
1112	9	-0.7461034792744101
1112	10	-0.8387121488239799
1112	11	-0.8760045391545788
1112	12	-0.9210542071909558
1112	13	-0.9118451246152333
1112	14	-0.6900442704808114
1112	15	-0.9385784704432822
1112	16	-0.8721897698940779
1112	17	-1.0269412487183587
1112	18	-0.9998845200172966
1112	19	-0.8444302267355965
1112	20	-0.9452475340301518
1112	21	-1.6578447579691726
1112	22	-1.7634876110453135
1112	23	-1.246341234836855
1112	24	-1.5789798203766523
1112	25	-1.9345352372665587
1113	1	0.02718057005220942
1113	2	-0.2305112468048094
1113	3	-0.19787912773635696
1113	4	0.024223831789655037
1113	5	0.04190334521114636
1113	6	-0.2238689591455767
1113	7	-0.04707801570559411
1113	8	-0.01381411258829246
1113	9	0.14974659783465682
1113	10	-0.07964394075552406
1113	11	-0.17282104556171163
1113	12	-0.0880945962312012
1113	13	-0.19109771884485127
1113	14	-0.10533778513413239
1113	15	-0.21971585190514276
1113	16	-0.02877788535369774
1113	17	-0.13806860015510836
1113	18	-0.02352536716465181
1113	19	-0.3011992594228232
1113	20	-0.6531853504729028
1113	21	-0.6776282679322918
1113	22	-0.12886823880535303
1113	23	-0.2852935259570373
1113	24	0.006344511918598528
1113	25	-1.1219921841632967
1114	1	-0.8953937639102953
1114	2	-0.6397699198849907
1114	3	-0.4913089046797623
1114	4	-1.5000105641468693
1114	5	-0.6441869023541429
1114	6	-0.6150546333662064
1114	7	-0.42682320366071025
1114	8	-0.45961750767342124
1114	9	-0.34465205113377095
1114	10	-0.4615608656472645
1114	11	-0.44313880871592204
1114	12	-0.5519377843562907
1114	13	-0.5342704496660104
1114	14	-0.6008015648368001
1114	15	-1.1170481650607016
1114	16	-0.7775758601294882
1114	17	-0.8392014656723035
1114	18	-0.7871065473783645
1114	19	-0.8059450198920715
1114	20	-1.9656364641273854
1114	21	-6.456971779973152
1114	22	-7.565346778176771
1114	23	-7.879897923781584
1114	24	-5.888324083823829
1114	25	-7.255853239818286
1115	1	-1.1874794313000514
1115	2	-1.0994293538637763
1115	3	-0.9528924361715383
1115	4	-0.7178348491965494
1115	5	-0.7844111240540315
1115	6	-1.1048231096896046
1115	7	-1.1253808792514235
1115	8	-0.816879852696772
1115	9	-2.744446697315311
1115	10	-1.6490097397545398
1115	11	-1.232202163325752
1115	12	-1.1086519608255543
1115	13	-1.5856830154110853
1115	14	-1.2208023313286205
1115	15	-2.0769923685170752
1115	16	-1.4948808596612224
1115	17	-3.2358934697159256
1115	18	-2.7815392025694763
1115	19	-2.0911910168124024
1115	20	-1.8778513234596375
1115	21	-1.3265303542882165
1115	22	-0.917071473604139
1115	23	-0.3291348456418035
1115	24	-1.3472017976680704
1115	25	-1.1410171561244837
1116	1	-1.6330325736228808
1116	2	-0.7785766627281845
1116	3	-0.5769966479941147
1116	4	-0.2517172267827661
1116	5	-0.25624711225070484
1116	6	-0.19735202145933073
1116	7	-0.6145756696683691
1116	8	-1.6855757739400659
1116	9	-0.7481803236296045
1116	10	-2.256304857463867
1116	11	-1.049018559834515
1116	12	-0.5926315623089948
1116	13	-0.7368639320141597
1116	14	-1.099072750344746
1116	15	-1.199965077842272
1116	16	-0.8049759788949515
1116	17	-0.6084344587135249
1116	18	-1.0927682830196233
1116	19	-0.6966139429167058
1116	20	-0.8755862900216798
1116	21	-0.4767449328447171
1116	22	-0.11369849219709494
1116	23	-0.03342495383404298
1116	24	0.025789321315862423
1116	25	-0.10077351919713351
1201	1	0.2516913126533602
1201	2	0.3661924038374238
1201	3	0.39490582898464766
1201	4	0.48485326338396817
1201	5	0.49103483192707387
1201	6	0.24391262419567994
1201	7	0.3295894605722154
1201	8	0.46455694979752593
1201	9	0.7355662587131917
1201	10	0.700175856231553
1201	11	0.48909619575286456
1201	12	0.49164095426330334
1201	13	0.40438183495380997
1201	14	1.0745011961169197
1201	15	0.7462065591872289
1201	16	0.8522301039828974
1201	17	0.7587103795236558
1201	18	0.5909501280181573
1201	19	0.7397016500403524
1201	20	0.8613427694340317
1201	21	0.7493330737224397
1201	22	0.8740463612995413
1201	23	0.8082368010202643
1201	24	0.7586891575142545
1201	25	0.8179255757717456
1202	1	-0.15274516427312435
1202	2	0.1422867702458923
1202	3	0.11876874396368109
1202	4	0.1411258955558523
1202	5	0.21907547819013473
1202	6	0.045348565649319994
1202	7	0.05320276970495996
1202	8	0.1937549724224965
1202	9	0.225617292553288
1202	10	0.45703567063407746
1202	11	0.22996633415123569
1202	12	0.2937507073199228
1202	13	0.33285870779241833
1202	14	0.7208005224543328
1202	15	0.5167879893070335
1202	16	0.47417038336262607
1202	17	0.4031576553179974
1202	18	0.329005556908065
1202	19	0.3007597105954787
1202	20	0.5678693786590685
1202	21	0.5015976569129634
1202	22	0.5169721590696312
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2211	4	0.27506215739909123
2211	5	0.25364062632648654
2211	6	0.21658734185783146
2211	7	0.2432137427678569
2211	8	0.26505824978927706
2211	9	0.2908145913706335
2211	10	0.38396844390857865
2211	11	0.13740717203700825
2211	12	0.13589395495323942
2211	13	0.10660516317846458
2211	14	0.3839668213857408
2211	15	0.2784109800369592
2211	16	0.16671224028804232
2211	17	0.3658315429679888
2211	18	0.3250056984959713
2211	19	0.31139650221347637
2211	20	0.5034272153531418
2211	21	0.4464275351080289
2211	22	0.47384684197170657
2211	23	0.6358575440081182
2211	24	0.6304745087753645
2211	25	0.7623313979090014
2212	1	-0.20553200148195216
2212	2	-0.3358251472788467
2212	3	-0.26592250991601674
2212	4	-0.6208646845294012
2212	5	-0.17433271172488674
2212	6	-0.6538681456458448
2212	7	-0.4194254404757345
2212	8	-0.3445437575529624
2212	9	-0.30574609087142335
2212	10	-0.7198221754722809
2212	11	-0.6854743210722631
2212	12	-0.7711976616156164
2212	13	-0.7327744144816108
2212	14	-0.5367408177828636
2212	15	-0.6857086975803313
2212	16	-0.5485159468257663
2212	17	-0.6155813428876371
2212	18	-0.4680835431584711
2212	19	-0.545133864108081
2212	20	-0.4495429543331966
2212	21	-0.4732643450520655
2212	22	-0.43594530909928153
2212	23	0.05545907870976663
2212	24	-0.13226200496907126
2212	25	-0.9370524544297112
2213	1	-0.16095061092497076
2213	2	-1.348011778292605
2213	3	-0.3826992087634622
2213	4	-0.03670964401001697
2213	5	-0.0032022106497890945
2213	6	0.014120859882517323
2213	7	0.08889394432853237
2213	8	0.0579622790124219
2213	9	0.02647725889503505
2213	10	-0.3618605096711036
2213	11	-0.15472887465933383
2213	12	-0.09849412445577599
2213	13	-0.3833030957694703
2213	14	0.024997517778771794
2213	15	-0.16140022019016698
2213	16	-0.1380236018883565
2213	17	0.17365846787206607
2213	18	0.09976712632007434
2213	19	0.07807909184304407
2213	20	0.18713971424060105
2213	21	-0.36061901439717303
2213	22	-0.9887437715167664
2213	23	-0.11535817796492864
2213	24	0.13041986747577994
2213	25	-0.5006393387646213
2214	1	-0.2056269144661549
2214	2	-0.4951649717798716
2214	3	-0.4930573517188179
2214	4	-0.5516188925829297
2214	5	-0.20242548766621127
2214	6	-0.2438106375819089
2214	7	-0.47183381576287786
2214	8	-0.11776575655514421
2214	9	-0.022075593001794402
2214	10	-1.7996127799539323
2214	11	-0.7895955322233092
2214	12	-0.5141378164111572
2214	13	-0.33662162525380523
2214	14	-0.772918205681492
2214	15	-0.48093423430891846
2214	16	-0.7619587068926776
2214	17	-0.49869043345716335
2214	18	-0.4956860798630487
2214	19	-1.4329403136554575
2214	20	-2.562628406700618
2214	21	-1.8236171172048756
2214	22	-2.000233881922817
2214	23	-2.5605029107410857
2214	24	-1.683455387339663
2214	25	-1.8238205972834223
2215	1	-0.2776444418490236
2215	2	-0.4271947084582379
2215	3	-0.8450442971643994
2215	4	-0.2951872939869986
2215	5	-0.14579490103304238
2215	6	-0.153673459103139
2215	7	-0.24151543490329885
2215	8	-0.15342922610821574
2215	9	-0.22807018099677379
2215	10	-0.33996596904540155
2215	11	-0.5318854795120203
2215	12	-0.4377569333503786
2215	13	-0.23045475705549734
2215	14	-0.17684278980236456
2215	15	-0.6283726301953294
2215	16	-0.3202583863986703
2215	17	-0.26931416410132414
2215	18	-0.31964095242852864
2215	19	-2.0211495387173883
2215	20	-2.1722555535995696
2215	21	-3.105423212460856
2215	22	-2.2354395028946286
2215	23	-3.262463148352758
2215	24	-2.4092528069520185
2215	25	-1.6320351639121355
2216	1	-1.5048087359840956
2216	2	-1.1785818488828
2216	3	-0.866388483212237
2216	4	-0.8769051891111346
2216	5	-1.8337834724322306
2216	6	-1.4238240665117914
2216	7	-1.0633699202034848
2216	8	-5.254877882959104
2216	9	-4.117437451629911
2216	10	-2.088087925539135
2216	11	-1.0844957498843542
2216	12	-1.0426936158510642
2216	13	-1.9180532340418992
2216	14	-3.440006662306395
2216	15	-1.8678517501747862
2216	16	-2.9724527103192884
2216	17	-2.553337118426626
2216	18	-2.485355092865376
2216	19	-1.4943105232201574
2216	20	-0.802148566150521
2216	21	-0.6111642790102891
2216	22	-0.6414655131880309
2216	23	-0.9670928914520474
2216	24	-0.4395223737318332
2216	25	-0.29293308377995686
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	3.0
7	47.0
8	209.0
9	564.0
10	1114.0
11	2204.0
12	3849.0
13	6002.0
14	7697.0
15	9762.0
16	11077.0
17	12128.0
18	12703.0
19	13209.0
20	13632.0
21	14929.0
22	15883.0
23	16726.0
24	18126.0
25	19594.0
26	21154.0
27	23657.0
28	27246.0
29	32130.0
30	39090.0
31	47710.0
32	63262.0
33	90128.0
34	131383.0
35	206582.0
36	359458.0
37	1184364.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.86223362850699	20.512018734989056	12.828078532603271	23.797669103900684
2	15.70835221579641	20.552961671025223	40.12501922893029	23.613666884248076
3	18.814007453356236	25.02071647185075	29.46350054945946	26.701775525333556
4	12.281615062048203	16.683056235277974	38.50244388218638	32.532884820487446
5	12.764394231081077	38.382828293748176	35.72755554409807	13.125221931072678
6	31.615367570423754	38.46482273448565	17.04475299980918	12.875056695281414
7	26.916236965547974	32.517871187770936	22.56637572810852	17.99951611857257
8	24.127839546254577	37.86532800017293	20.495822625581045	17.511009827991444
9	24.16586193608409	17.18343326126439	21.74925911437927	36.90144568827225
10	14.044698371418916	30.868007071858848	34.27561044250665	20.81168411421559
11	32.83818168742593	25.311472056993843	23.131676887985623	18.7186693675946
12	20.60320788569205	27.379309370079135	31.5872898420833	20.43019290214552
13	26.870020394402637	23.532130953182666	26.6282057997258	22.969642852688892
14	20.23249027479978	23.455170963158555	28.157645100652395	28.154693661389274
15	22.44024817351361	31.43136256404394	23.999127027841578	22.129262234600873
16	21.54999503236033	31.188878805804364	25.835924889288147	21.42520127254716
17	20.158008289095726	30.276483328275628	28.17494398417007	21.39056439845858
18	20.38127568013382	29.895915954565183	29.173837566034628	20.548970799266364
19	20.978861096469423	29.218427576849464	29.317903302777005	20.484808023904105
20	21.846267682625196	28.91686516101945	28.91503610310898	20.32183105324637
21	21.923313416865454	28.78084923863401	28.28357544558278	21.012261898917757
22	21.1038364031048	28.651047815881935	29.16711388232279	21.078001898690466
23	21.419080266584572	28.762802965378953	28.70202268132161	21.11609408671487
24	21.24460736100938	28.71544408574348	28.869460100083387	21.170488453163752
25	21.5296219420435	28.724397074594183	28.36647065636647	21.37951032699584
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1793.0
1	1793.0
2	2071.0
3	2349.0
4	2349.0
5	2349.0
6	3508.0
7	4667.0
8	4667.0
9	4667.0
10	7393.0
11	10119.0
12	10119.0
13	10119.0
14	16726.5
15	23334.0
16	23334.0
17	23334.0
18	36417.0
19	49500.0
20	49500.0
21	49500.0
22	70272.5
23	91045.0
24	91045.0
25	91045.0
26	118178.5
27	145312.0
28	145312.0
29	145312.0
30	172978.0
31	200644.0
32	200644.0
33	200644.0
34	222919.0
35	245194.0
36	245194.0
37	245194.0
38	257464.0
39	269734.0
40	269734.0
41	269734.0
42	271724.5
43	273715.0
44	273715.0
45	273715.0
46	273931.5
47	274148.0
48	274148.0
49	274148.0
50	261541.5
51	248935.0
52	248935.0
53	248935.0
54	229494.0
55	210053.0
56	210053.0
57	210053.0
58	183458.5
59	156864.0
60	156864.0
61	156864.0
62	130058.0
63	103252.0
64	103252.0
65	103252.0
66	79020.5
67	54789.0
68	54789.0
69	54789.0
70	40044.5
71	25300.0
72	25300.0
73	25300.0
74	17579.5
75	9859.0
76	9859.0
77	9859.0
78	6660.5
79	3462.0
80	3462.0
81	3462.0
82	2294.0
83	1126.0
84	1126.0
85	1126.0
86	723.0
87	320.0
88	320.0
89	320.0
90	202.5
91	85.0
92	85.0
93	85.0
94	51.0
95	17.0
96	17.0
97	17.0
98	11.5
99	6.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.029514196328433977
2	0.01629516191654383
3	0.02207329331042034
4	0.02647963811438372
5	0.02477529719964317
6	0.009103674642150762
7	0.0030761275046536822
8	0.0018290487865508381
9	0.0022863109831885472
10	0.0012886480087062721
11	0.001205509427499416
12	0.0020784645301714067
13	0.002494157436205688
14	6.651086496548502E-4
15	0.0019537566583611226
16	0.002203172401981691
17	0.002161603111378263
18	0.0027435731798262568
19	4.1569290603428135E-4
20	4.988314872411377E-4
21	0.07964676079616831
22	0.07731888052237634
23	0.07823340491565176
24	0.0011639401368959878
25	0.22480672358333934
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2405622.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.24004260548219
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.5654528992371	76.29237610816524
2	7.325946281817945	12.34276053811635
3	1.1596323180850865	2.9306242764654526
4	0.3526104319838649	1.18815671253833
5	0.15891663120300725	0.6693571891630514
6	0.10177351035681832	0.5144042909140716
7	0.0645923885129476	0.38088858922243946
8	0.04544074679346252	0.30623443567249686
9	0.03401457370134918	0.2578850224028072
>10	0.17622695113803688	2.581161643406972
>50	0.009542453558135051	0.5497186365235387
>100	0.00461398035787031	0.715637158411718
>500	7.420999526787757E-4	0.4494378060737018
>1k	4.947333017851607E-4	0.8213575929238023
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	3169	0.13173308192226377	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3133	0.13023658746054034	No Hit
CCCATGTACTCTGCGTTGATACCAC	2877	0.11959484906606274	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2469	0.10263457849986407	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	4.1569290603428136E-5	0.0	0.0
7	0.0	0.0	4.1569290603428136E-5	0.0	0.0
8	0.0	0.0	4.1569290603428136E-5	0.0	0.0
9	0.0	0.0	4.1569290603428136E-5	0.0	0.0
10	0.0	0.0	4.1569290603428136E-5	0.0	0.0
11	0.0	0.0	4.1569290603428136E-5	4.1569290603428136E-5	0.0
12	0.0	0.0	4.1569290603428136E-5	4.1569290603428136E-5	0.0
13	0.0	0.0	4.1569290603428136E-5	4.1569290603428136E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCTG	85	5.341073E-5	11.173254	5
GTGTAGG	265	0.0	10.756766	1
GTCTAGA	345	0.0	10.741176	1
GTCCAGG	470	0.0	10.714807	1
TAGACTG	345	0.0	10.460756	5
GTCTTAC	205	3.6379788E-12	10.197063	1
TAGGACT	305	0.0	9.965588	4
GTCTCGC	125	2.5649333E-6	9.881882	1
TCTAGAC	270	0.0	9.850655	3
CTAGTAC	185	6.511982E-10	9.755571	3
ACTGATC	265	0.0	9.67465	8
GTCTAAA	210	6.730261E-11	9.50181	1
GCCTAAG	200	2.6375346E-10	9.50181	1
CTAGACT	310	0.0	9.49845	4
CTACACT	400	0.0	9.49845	4
GGACAGT	300	0.0	9.49628	6
ACTGACC	290	0.0	9.495489	8
CTAGGAC	265	0.0	9.319623	3
CTAGACA	265	0.0	9.319235	4
CCTAGTA	235	7.2759576E-12	9.2977085	2
>>END_MODULE