FastQCFastQC Report
Wed 25 May 2016
SRR1294771_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294771_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2405622
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT90480.3761189413798178No Hit
GGTATCAACGCAGAGTACTTTTTTT75390.31339088185924474No Hit
TATCAACGCAGAGTACTTTTTTTTT60810.2527828561594465No Hit
ACGCAGAGTACTTTTTTTTTTTTTT40240.16727482538819483No Hit
GTACATGGGAAGCAGTGGTATCAAC34670.14412073052208535No Hit
CCCATGTACTCTGCGTTGATACCAC30740.12778399931493808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGT504.5814613E-617.0933026
ACCGTGA602.582971E-514.2420498
TAGACTG3700.011.8285625
TATACCG600.005811905511.0999915
AGACCGT600.00589440711.0789926
CTAGGAC2350.010.9312983
TAGACAG3050.010.9180255
TACCGTG1053.4914501E-610.8510867
CTAGCAC2850.010.6826983
CCTAGAC1659.058567E-1010.3792123
GACCGTG856.629666E-410.0532117
TAGGACT2950.09.9980554
TCTATAC3150.09.6652983
GGTTAGA2201.6370905E-119.5182441
CTAGAAC2700.09.5142783
TATACTG4500.09.5142785
CTAGATA2303.6379788E-129.5142774
GCACCGT900.00111797939.4962786
ACCGTCT1002.7717365E-49.49469958
CCGTCTG900.00112031989.4941079
Produced by FastQC (version 0.11.5)