FastQCFastQC Report
Wed 25 May 2016
SRR1294770_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294770_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4777926
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC808941.6930777077752983No Hit
CCCATGTACTCTGCGTTGATACCAC779011.630435465095106No Hit
GAGTACATGGGAAGCAGTGGTATCA373680.7820966670475851No Hit
CATGTACTCTGCGTTGATACCACTG299540.6269247368000258No Hit
GCGTTGATACCACTGCTTCCCATGT279220.5843958236272391No Hit
GCTTCCCATGTACTCTGCGTTGATA223760.46832035489875734No Hit
GTATCAACGCAGAGTACATGGGAAG206140.43144242920463816No Hit
ACGCAGAGTACATGGGAAGCAGTGG202600.4240333567326074No Hit
ACTCTGCGTTGATACCACTGCTTCC169020.3537518161645869No Hit
TATCAACGCAGAGTACATGGGAAGC158420.3315664579150033No Hit
GGTATCAACGCAGAGTACATGGGAA130860.2738845264660859No Hit
GTGGTATCAACGCAGAGTACATGGG106570.22304656874133255No Hit
CAGTGGTATCAACGCAGAGTACATG105730.22128848374796933No Hit
GCAGTGGTATCAACGCAGAGTACAT104830.21940482125508012No Hit
ATACCACTGCTTCCCATGTACTCTG85820.1796176834886099No Hit
GTACTCTGCGTTGATACCACTGCTT78080.16341818604976302No Hit
GCAGAGTACATGGGAAGCAGTGGTA77760.16274843938562464No Hit
CATGGGAAGCAGTGGTATCAACGCA75850.15875088898404874No Hit
ACATGGGAAGCAGTGGTATCAACGC73450.15372778900301093No Hit
CTGCTTCCCATGTACTCTGCGTTGA73190.1531836198383985No Hit
GGGAAGCAGTGGTATCAACGCAGAG70930.14845353402292125No Hit
GTACATGGTAAGCAGTGGTATCAAC70200.1469256744453556No Hit
TACCACTGCTTCCCATGTACTCTGC67590.14146305321597696No Hit
CTTCCCATGTACTCTGCGTTGATAC62480.13076803617301733No Hit
GATACCACTGCTTCCCATGTACTCT61780.12930296534521463No Hit
AAGCAGTGGTATCAACGCAGAGTAC60450.12651933077238953No Hit
GTTGATACCACTGCTTCCCATGTAC59940.125451922026419No Hit
CCACTGCTTCCCATGTACTCTGCGT57930.12124507579229983No Hit
CCATGTACTCTGCGTTGATACCACT56090.11739403247350419No Hit
ACCATGTACTCTGCGTTGATACCAC50310.10529673335250482No Hit
ATGGGAAGCAGTGGTATCAACGCAG49080.10272239461222296No Hit
TTCCCATGTACTCTGCGTTGATACC48520.10155033794998082No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC550.003067616112.0921891
GTCGTAA550.003067616112.0921891
TACGAAA1803.45608E-1110.5602118
CGCAAAA2250.010.5549042
CTAGTAC2350.010.509553
GTCCTAG3000.09.8177051
GCACCGT1554.0901796E-89.8033836
AATACGA2002.6375346E-109.5039916
TAGGCCG1106.8582754E-59.4980225
TATACCG1201.7063834E-59.4980225
TGCACCG1304.25484E-69.4980225
GCACCGA1356.8821428E-69.1452876
GTCCAAG5300.09.1424761
GTCCTAA3900.09.0137741
CGAATTA950.00182071329.00227215
CCGTATA850.0074432328.9406252
TCCGGTA850.0074432328.9406252
TAGGCCC3300.08.9230384
ATACGAA2252.6011548E-108.87057717
CTAAGAC3400.08.6608683
Produced by FastQC (version 0.11.5)