FastQCFastQC Report
Wed 25 May 2016
SRR1294770_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294770_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4777926
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC867711.8160808685609615No Hit
GTACATGGGAAGCAGTGGTATCAAC813181.7019518510751317No Hit
GAGTACATGGGAAGCAGTGGTATCA409880.8578617584282385No Hit
CATGTACTCTGCGTTGATACCACTG348280.7289355255816018No Hit
GCGTTGATACCACTGCTTCCCATGT278010.5818633440534658No Hit
ACGCAGAGTACATGGGAAGCAGTGG258880.5418250512879438No Hit
GTATCAACGCAGAGTACATGGGAAG226060.4731341590472519No Hit
GCTTCCCATGTACTCTGCGTTGATA225450.47185745446873806No Hit
ACTCTGCGTTGATACCACTGCTTCC193150.4042549005572711No Hit
TATCAACGCAGAGTACATGGGAAGC186930.3912366997730814No Hit
GGTATCAACGCAGAGTACATGGGAA148660.31113918465878293No Hit
CAGTGGTATCAACGCAGAGTACATG131360.2749310056288021No Hit
GTATCAACGCAGAGTACTTTTTTTT121650.25460838028885335No Hit
GTGGTATCAACGCAGAGTACATGGG114740.24014603826011538No Hit
GCAGTGGTATCAACGCAGAGTACAT99040.20728659255082643No Hit
ATACCACTGCTTCCCATGTACTCTG98750.20667963463645106No Hit
CATGGGAAGCAGTGGTATCAACGCA98020.20515177505888538No Hit
ACATGGGAAGCAGTGGTATCAACGC96100.20113329507405517No Hit
GGTATCAACGCAGAGTACTTTTTTT90360.18911971428607308No Hit
TATCAACGCAGAGTACTTTTTTTTT88940.18614771346395906No Hit
GGGAAGCAGTGGTATCAACGCAGAG86950.18198272639634855No Hit
CTGCTTCCCATGTACTCTGCGTTGA81080.16969706102606025No Hit
GTACTCTGCGTTGATACCACTGCTT77520.16224612938752087No Hit
CTTCCCATGTACTCTGCGTTGATAC77180.16153452355687384No Hit
AAGCAGTGGTATCAACGCAGAGTAC75130.1572439589897374No Hit
TACCACTGCTTCCCATGTACTCTGC74630.15619747982702117No Hit
GATACCACTGCTTCCCATGTACTCT70980.14855818193919285No Hit
GCAGAGTACATGGGAAGCAGTGGTA69860.14621406861470856No Hit
CCACTGCTTCCCATGTACTCTGCGT67820.14194443363082643No Hit
GTACATGGTAAGCAGTGGTATCAAC67010.14024913738722616No Hit
CCATGTACTCTGCGTTGATACCACT66520.13922358780776428No Hit
GTTGATACCACTGCTTCCCATGTAC65290.1366492490674824No Hit
ATGGGAAGCAGTGGTATCAACGCAG61360.12842392284853302No Hit
ACCATGTACTCTGCGTTGATACCAC57040.11938234288266499No Hit
ACGCAGAGTACTTTTTTTTTTTTTT53570.11211977749341451No Hit
TTCCCATGTACTCTGCGTTGATACC50910.1065525083477643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAAA1658.185452E-1110.93360918
TACGCAG1502.0918378E-910.7809435
CGAACGA1553.6925485E-910.4154819
TCGAACG1356.498667E-79.846642518
GTCCTAG4000.09.7553211
TCTACGC1502.6015914E-79.5125973
CTAGCAC3800.09.5125973
TAGGCCG1002.72107E-49.5125965
ATACCGT800.0045345739.4958726
CGCGAGA800.00453809749.49497714
GTTCTAG3900.09.2733511
AGTACCG1252.7140257E-59.1320925
GTCCTAA3350.09.0912351
GTCTAGA3700.09.00293351
TACGTCT850.0073590458.95303254
TAGACTG5150.08.8661095
GTCCTAC3250.08.785281
GTGTTAG4550.08.7852791
TGCACCG1304.3052525E-58.7808585
TGGACCG1201.7400213E-48.7198815
Produced by FastQC (version 0.11.5)