FastQCFastQC Report
Wed 25 May 2016
SRR1294769_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294769_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4401460
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1227812.7895516487710896No Hit
CCCATGTACTCTGCGTTGATACCAC1189172.7017625969564643No Hit
GAGTACATGGGAAGCAGTGGTATCA576401.3095654623693047No Hit
CATGTACTCTGCGTTGATACCACTG462931.0517646417325162No Hit
GCGTTGATACCACTGCTTCCCATGT386110.8772316458629636No Hit
GCTTCCCATGTACTCTGCGTTGATA315020.7157170575218222No Hit
ACGCAGAGTACATGGGAAGCAGTGG314840.7153081023115058No Hit
GTATCAACGCAGAGTACATGGGAAG309430.703016726268102No Hit
ACTCTGCGTTGATACCACTGCTTCC259160.5888046239202446No Hit
TATCAACGCAGAGTACATGGGAAGC239430.5439785889227665No Hit
GGTATCAACGCAGAGTACATGGGAA202600.4603018089452136No Hit
CAGTGGTATCAACGCAGAGTACATG164530.37380778196325765No Hit
GTGGTATCAACGCAGAGTACATGGG153530.3488160746661335No Hit
GCAGTGGTATCAACGCAGAGTACAT131050.29774211284437435No Hit
ATACCACTGCTTCCCATGTACTCTG130990.29760579444093554No Hit
GTACTCTGCGTTGATACCACTGCTT120720.27427262771898414No Hit
CATGGGAAGCAGTGGTATCAACGCA117380.266684236594221No Hit
CTGCTTCCCATGTACTCTGCGTTGA113840.25864145079132833No Hit
GTACATGGTAAGCAGTGGTATCAAC112650.25593780245645764No Hit
ACATGGGAAGCAGTGGTATCAACGC112360.25527893017316977No Hit
GGGAAGCAGTGGTATCAACGCAGAG109400.2485538889368528No Hit
TACCACTGCTTCCCATGTACTCTGC101020.22951475192322549No Hit
CTTCCCATGTACTCTGCGTTGATAC95800.21765505082404474No Hit
GTTGATACCACTGCTTCCCATGTAC94800.21538307743339707No Hit
GATACCACTGCTTCCCATGTACTCT93640.21274758830024582No Hit
AAGCAGTGGTATCAACGCAGAGTAC92360.20983946236021686No Hit
GCAGAGTACATGGGAAGCAGTGGTA91710.20836267965629587No Hit
CCACTGCTTCCCATGTACTCTGCGT88390.20081972799934567No Hit
CCATGTACTCTGCGTTGATACCACT86060.19552602999913668No Hit
ACCATGTACTCTGCGTTGATACCAC78020.17725936393832956No Hit
GTACATGGGTAAGCAGTGGTATCAA74170.16851226638433608No Hit
ATGGGAAGCAGTGGTATCAACGCAG72970.16578589831555893No Hit
TTCCCATGTACTCTGCGTTGATACC70660.16053763978316288No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA68750.15619817060702584No Hit
TGATACCACTGCTTCCCATGTACTC65430.14865521895007566No Hit
CCCCATGTACTCTGCGTTGATACCA54450.12370895112076448No Hit
GGTAAGCAGTGGTATCAACGCAGAG52430.11911956487165622No Hit
ATCAACGCAGAGTACATGGGAAGCA47000.1067827493604395No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTC500.001505083213.2943937
ACCGTCC701.096082E-412.2091368
CCGTCCA1001.941593E-611.3953249
GGACCGC600.005895040511.0791656
GTACAAA9550.011.0440051
CGCAAAA2950.010.3044862
TAGGCCG856.611928E-410.0565265
CAGGACG951.6480396E-49.9983974
GGTATCG1356.407445E-79.85676917
TAGACAG5250.09.7691965
GTTCTAG2650.09.6811051
GTCCTAG3150.09.6526481
CTACACT4450.09.6052014
CCGAGAC1002.760596E-49.4989093
TATTCCG1502.660745E-79.4978295
GAACAAA14950.09.4064881
GTATTAG4750.09.4018061
TATACTG7600.09.2478875
GACTGTC2800.09.1568537
CTAGACA4150.09.1551584
Produced by FastQC (version 0.11.5)