##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294769_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4401460
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.95668800807005	33.0	33.0	33.0	27.0	33.0
2	31.007002449187315	33.0	33.0	33.0	27.0	33.0
3	30.93908952938343	33.0	33.0	33.0	27.0	33.0
4	34.260092560195936	37.0	37.0	37.0	27.0	37.0
5	34.68870602027509	37.0	37.0	37.0	27.0	37.0
6	34.81303203936876	37.0	37.0	37.0	33.0	37.0
7	34.834332471498094	37.0	37.0	37.0	33.0	37.0
8	34.71606625983196	37.0	37.0	37.0	27.0	37.0
9	34.409178772498215	37.0	37.0	37.0	27.0	37.0
10	34.52157375052823	37.0	37.0	37.0	27.0	37.0
11	34.797775510853214	37.0	37.0	37.0	33.0	37.0
12	34.729034456748444	37.0	37.0	37.0	27.0	37.0
13	34.607937593434904	37.0	37.0	37.0	27.0	37.0
14	35.815153608120944	40.0	37.0	40.0	27.0	40.0
15	36.05861873105742	40.0	37.0	40.0	27.0	40.0
16	36.06238861650452	40.0	37.0	40.0	27.0	40.0
17	36.04522317594616	40.0	37.0	40.0	27.0	40.0
18	36.03611142666297	40.0	37.0	40.0	27.0	40.0
19	36.038583333712	40.0	37.0	40.0	27.0	40.0
20	35.961073598306015	40.0	37.0	40.0	27.0	40.0
21	35.88567907012673	40.0	37.0	40.0	27.0	40.0
22	35.79235299196176	40.0	37.0	40.0	27.0	40.0
23	35.44836895030286	37.0	37.0	40.0	27.0	40.0
24	35.78074547990894	37.0	37.0	40.0	27.0	40.0
25	34.67558969069354	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.4265346938926946
1101	2	0.587431475365797
1101	3	0.350970248466691
1101	4	0.4600887640967315
1101	5	0.5399312660356159
1101	6	0.4023235402318548
1101	7	0.45821546282866166
1101	8	0.5106961717890002
1101	9	0.7722929926125346
1101	10	0.7564133335214294
1101	11	0.5039360376040065
1101	12	0.5076794645960376
1101	13	0.5336429148525923
1101	14	1.2229401253965761
1101	15	0.9591690058619591
1101	16	0.9274969777158404
1101	17	0.9477786415672682
1101	18	0.8764617313729772
1101	19	0.89218262718029
1101	20	0.9288650038951829
1101	21	0.9553347554588569
1101	22	0.9229559691965505
1101	23	1.2652863081964654
1101	24	1.0904637074508798
1101	25	0.8166367351966244
1102	1	0.23219586900290068
1102	2	0.4725996172392328
1102	3	0.2175264005106392
1102	4	0.18785892273820792
1102	5	0.2722152882858069
1102	6	0.1489448288746189
1102	7	0.2012654980186639
1102	8	0.36284679063951586
1102	9	0.5141433646624733
1102	10	0.6399831077225926
1102	11	0.39991699686235194
1102	12	0.40785416817200826
1102	13	0.36955750920498787
1102	14	1.0659334174198278
1102	15	0.7838811585246859
1102	16	0.5390333736613044
1102	17	0.6475689048228759
1102	18	0.692938778126404
1102	19	0.6565655010788731
1102	20	0.8776504574368644
1102	21	0.8027676183208428
1102	22	0.9352412692823862
1102	23	1.265639859234028
1102	24	0.883991882653703
1102	25	0.8626701864243316
1103	1	0.186523917568703
1103	2	0.4422191649485008
1103	3	0.12477668157067967
1103	4	-0.02326389680710861
1103	5	0.1821092796561885
1103	6	0.14524079375799914
1103	7	0.20113075320933405
1103	8	0.35404539261656254
1103	9	0.5796939273171873
1103	10	0.5512663802505884
1103	11	0.3189582795043009
1103	12	0.3627027047423468
1103	13	0.3384672883631197
1103	14	0.9176177583551777
1103	15	0.7405275788993819
1103	16	0.4571711853889866
1103	17	0.5285505137911315
1103	18	0.6057713479728193
1103	19	0.5958287422955877
1103	20	0.703178708409304
1103	21	0.7366306691957689
1103	22	0.7137199183804483
1103	23	1.098220827183603
1103	24	0.7649354639997625
1103	25	0.6298701750830418
1104	1	0.20686991664409504
1104	2	0.4062614131752085
1104	3	0.168652500515325
1104	4	0.135983470416015
1104	5	0.16459941298918324
1104	6	0.0918732443730832
1104	7	0.17929112031833938
1104	8	0.34085636848973166
1104	9	0.5259552197425634
1104	10	0.5348736762647803
1104	11	0.2810262013756457
1104	12	0.20337169732679428
1104	13	0.363782747678151
1104	14	0.7438202473150213
1104	15	0.5658412070538645
1104	16	0.5406599588918368
1104	17	0.5704353196075189
1104	18	0.5179958276234018
1104	19	0.5167760712835658
1104	20	0.5630888396919147
1104	21	0.7050011877688291
1104	22	0.7006912626521711
1104	23	0.9479326321727441
1104	24	0.8175817424560918
1104	25	0.6027871815997798
1105	1	0.13553600502995167
1105	2	0.2237736794981977
1105	3	0.21435943615216502
1105	4	0.0933238848421638
1105	5	0.13383036910753532
1105	6	0.1283081241963373
1105	7	0.11705441365919
1105	8	0.2342333575954072
1105	9	-1.7099956504308622
1105	10	-0.19445575209135058
1105	11	-0.05589748390546845
1105	12	0.09928585868991746
1105	13	0.16934712507407568
1105	14	-1.7595505477325162
1105	15	-0.37793909179204377
1105	16	-0.1368794499330832
1105	17	0.1513927476371819
1105	18	0.09392479398757558
1105	19	0.22066240936779025
1105	20	0.3543857261495731
1105	21	0.4187113400135942
1105	22	0.47523157053292664
1105	23	0.8380888628953969
1105	24	0.5970280227628493
1105	25	0.1942004075272621
1106	1	0.013418666143405034
1106	2	0.051160333194467
1106	3	0.023951305296201042
1106	4	-1.3618861611117907
1106	5	-0.40467323318476645
1106	6	-0.12680490064279581
1106	7	-0.006290005858843983
1106	8	0.16651621562198216
1106	9	0.26514112123649625
1106	10	0.29864692453399755
1106	11	0.13153753378151123
1106	12	0.10359454207213759
1106	13	0.10712102160432835
1106	14	0.5591800576771249
1106	15	0.3241484411684681
1106	16	0.12876915231162656
1106	17	0.09220359695799374
1106	18	0.15090940010164644
1106	19	0.19086381166362543
1106	20	0.3522412437136282
1106	21	0.4132459884582431
1106	22	0.5169102307379632
1106	23	0.5996836869450917
1106	24	0.338848429592737
1106	25	0.21092374413492365
1107	1	0.3688577914854818
1107	2	0.34532919167812537
1107	3	0.36525010135603253
1107	4	0.47600980796985226
1107	5	0.4506253057697549
1107	6	0.2831244671361688
1107	7	0.28848568827054066
1107	8	0.31526222554309413
1107	9	0.5396664165460123
1107	10	0.4625030783935884
1107	11	0.35472606082147706
1107	12	0.39096763971557635
1107	13	0.39990397600817573
1107	14	0.774239104538438
1107	15	0.6709350818283895
1107	16	0.5651804250025876
1107	17	0.5759565546236729
1107	18	0.6274665910490853
1107	19	0.5744589770192547
1107	20	0.6245493239133637
1107	21	0.7209240995511053
1107	22	0.6695870476261021
1107	23	0.8463619430212859
1107	24	0.7709633128545406
1107	25	0.737356819865397
1108	1	0.43102561629082103
1108	2	0.37604933968314214
1108	3	0.5200555905823876
1108	4	0.6478794525647373
1108	5	0.6053783171507803
1108	6	0.45131929594742815
1108	7	0.395892094306717
1108	8	0.44310093532278927
1108	9	0.3058386945338398
1108	10	0.5284418190368143
1108	11	0.3205365805959488
1108	12	0.40795132905460463
1108	13	0.3867750577307163
1108	14	-0.9437640872078461
1108	15	-0.03151646444771927
1108	16	0.36829639546499493
1108	17	0.4413065485067236
1108	18	0.6268392074752853
1108	19	0.5368303939986987
1108	20	0.6514221352182261
1108	21	0.723457467534395
1108	22	0.7910885109380246
1108	23	1.075171307080545
1108	24	0.9202332640293776
1108	25	0.7571549069823362
1109	1	0.4718860794217292
1109	2	0.3832705412651478
1109	3	0.4609010142948584
1109	4	0.6037611642965146
1109	5	0.5714814446298178
1109	6	0.34208940405906674
1109	7	0.334265718270359
1109	8	0.41732682452560965
1109	9	0.6443269677899224
1109	10	0.6023111224210638
1109	11	0.42048165519088343
1109	12	0.39768087755856385
1109	13	0.41273178364887997
1109	14	0.8962179093158724
1109	15	0.5896950767563709
1109	16	0.5560147224967196
1109	17	0.618956447749774
1109	18	0.606640146036959
1109	19	0.6217033372978591
1109	20	0.7017744256572556
1109	21	0.7020245968931178
1109	22	0.7485498982485694
1109	23	1.1913404841256252
1109	24	0.9806767672219721
1109	25	0.8583595496414205
1110	1	0.3074170552492781
1110	2	0.20705550493146774
1110	3	0.2988753394446597
1110	4	0.42576502427581886
1110	5	0.3454658992939983
1110	6	0.18858177704892398
1110	7	0.15047481557637354
1110	8	0.24310940813263926
1110	9	0.308372233787388
1110	10	0.3540315866576478
1110	11	0.09532112124759351
1110	12	0.038637331361343286
1110	13	0.10181783349607088
1110	14	0.5890230852182157
1110	15	0.3078192880737305
1110	16	0.30998581564425365
1110	17	0.3274482229932474
1110	18	0.37349174296362975
1110	19	0.26877068002844595
1110	20	0.42538064195183267
1110	21	0.4594642903577508
1110	22	0.5946697864098383
1110	23	0.703405246130167
1110	24	0.5907508169899671
1110	25	0.5448301513788891
1111	1	0.22375736613900443
1111	2	0.12223506911008997
1111	3	0.3319699730942496
1111	4	0.595135328547066
1111	5	0.40591075649931696
1111	6	0.2991644134543634
1111	7	0.2175137241752907
1111	8	0.36220824566495224
1111	9	0.35736023467083555
1111	10	0.2515166954762975
1111	11	0.21125130013208349
1111	12	0.2067201589463039
1111	13	0.22433064518443757
1111	14	-1.7212664290314947
1111	15	-0.5252587742963684
1111	16	-0.042905842955221374
1111	17	0.2137285643910758
1111	18	0.2254003489807701
1111	19	0.2107265710804498
1111	20	0.2751908263147911
1111	21	0.41490033816202754
1111	22	0.16777919997092283
1111	23	-1.6747016053081083
1111	24	-0.3019854476999839
1111	25	0.15054788535525887
1112	1	-0.12111429250866834
1112	2	-0.1772242600924976
1112	3	0.0827828455386097
1112	4	0.3137899511524722
1112	5	-0.6451380663458295
1112	6	-0.7998002848519761
1112	7	-0.9692736889452149
1112	8	-0.7201209777429298
1112	9	-0.5855043539197524
1112	10	-0.8496567625139448
1112	11	-0.7641362944940724
1112	12	-0.7500884047950223
1112	13	-0.776014408515266
1112	14	-0.4865120256630675
1112	15	-0.7575186451076945
1112	16	-0.7981990786065438
1112	17	-0.6980048079389505
1112	18	-0.9472513411304249
1112	19	-0.7605716480122027
1112	20	-0.8969370624193758
1112	21	-1.713884695906053
1112	22	-1.7551827274286325
1112	23	-1.4616774960193766
1112	24	-1.6452770932076533
1112	25	-1.8821777116530285
1113	1	0.04144170039314332
1113	2	-0.08126130057058134
1113	3	-0.14911275783072142
1113	4	0.2012493934831454
1113	5	0.14143316664647898
1113	6	-0.05616019895671087
1113	7	-0.04545421557760676
1113	8	0.07919983560029209
1113	9	0.21458385885736675
1113	10	0.03315283993642737
1113	11	-0.04564439056447611
1113	12	-0.04816992806677689
1113	13	-0.1976362759731316
1113	14	0.04515325952191063
1113	15	-0.13172477883539813
1113	16	-0.010811341942009278
1113	17	0.012088431647789832
1113	18	0.029326884298136235
1113	19	-0.0827383812713336
1113	20	-0.6457371003622328
1113	21	-0.5461456012953221
1113	22	-0.03435717007139516
1113	23	-0.28850657289011394
1113	24	0.014575061143361268
1113	25	-1.0419952507944927
1114	1	-0.7769386386356238
1114	2	-0.6698682536341423
1114	3	-0.3915007777684174
1114	4	-1.4636278970895802
1114	5	-0.6646305933366037
1114	6	-0.4991109627427335
1114	7	-0.41399776978100533
1114	8	-0.41608661669580016
1114	9	-0.40122092541240306
1114	10	-0.466522806680004
1114	11	-0.3959040397390936
1114	12	-0.45903089917485573
1114	13	-0.5628598936744069
1114	14	-0.5327347220246992
1114	15	-1.2392263493681952
1114	16	-0.7613946217023368
1114	17	-0.7291025125540997
1114	18	-0.7128279686400134
1114	19	-0.7203625353886736
1114	20	-1.870554335575548
1114	21	-6.444500349484706
1114	22	-7.299506249890204
1114	23	-7.7437891997052155
1114	24	-5.536357321358299
1114	25	-6.904214297842955
1115	1	-1.0222224975696008
1115	2	-1.1253786836866055
1115	3	-0.8306782054267998
1115	4	-0.6248571174060586
1115	5	-0.7209209532151917
1115	6	-0.914764726665581
1115	7	-1.0278703915895946
1115	8	-0.7245469879061801
1115	9	-2.7446317479126883
1115	10	-1.6242152742130003
1115	11	-1.147928645731369
1115	12	-1.0622921197306496
1115	13	-1.4415104956833176
1115	14	-1.124080539418621
1115	15	-1.9803057934889736
1115	16	-1.3435297392310446
1115	17	-3.154625950657511
1115	18	-2.764301309754913
1115	19	-2.0586183878645556
1115	20	-1.7200036164754593
1115	21	-1.2820165867731674
1115	22	-0.842802894787674
1115	23	-0.24732027938636492
1115	24	-1.2530492663198984
1115	25	-1.0193967934285766
1116	1	-1.5164407224105538
1116	2	-0.81926147344425
1116	3	-0.5112257931996744
1116	4	-0.133382520254969
1116	5	-0.26187607274377456
1116	6	-0.29482930013109154
1116	7	-0.7236633306792228
1116	8	-1.63030525382257
1116	9	-0.797982836861685
1116	10	-2.3136917321645782
1116	11	-1.003114499286049
1116	12	-0.6964163991954138
1116	13	-0.6358921480808775
1116	14	-1.1481578857616057
1116	15	-1.2044300804308392
1116	16	-0.7239781214892105
1116	17	-0.6215396045676869
1116	18	-1.2334706016618853
1116	19	-0.7238451990934394
1116	20	-0.9149210808394486
1116	21	-0.4183202378339459
1116	22	-0.02048916846180049
1116	23	0.12692144161232477
1116	24	-0.03843429045254254
1116	25	0.06331034851484674
1201	1	0.187920961768814
1201	2	0.36599948231437907
1201	3	0.208191946133212
1201	4	0.2381810036442289
1201	5	0.2041384157162156
1201	6	0.10490689691348365
1201	7	0.23462809186976585
1201	8	0.3111478492887869
1201	9	0.5664062389451416
1201	10	0.485271307580291
1201	11	0.4006491188930852
1201	12	0.36389086961589356
1201	13	0.3874207182741074
1201	14	1.0422634208363988
1201	15	0.7438069181835161
1201	16	0.6825415546275835
1201	17	0.6758223182860092
1201	18	0.47909750559355757
1201	19	0.6217085336850658
1201	20	0.7177112329047404
1201	21	0.7501303588399821
1201	22	0.7481574124391628
1201	23	0.5414828126833271
1201	24	0.6878585427259978
1201	25	0.5932281641140591
1202	1	-0.1757251931129531
1202	2	0.09291779418710888
1202	3	-0.053336276403143756
1202	4	-0.07107053578141631
1202	5	0.012339042705605152
1202	6	-0.09065585491603656
1202	7	0.006312837371694968
1202	8	0.1369258161891338
1202	9	0.163776874300801
1202	10	0.4265778400019897
1202	11	0.14105523556568045
1202	12	0.161864132813605
1202	13	0.27057101942775574
1202	14	0.39573285830895344
1202	15	0.3884838391399015
1202	16	0.3386856339693054
1202	17	0.2525670541074234
1202	18	0.26182508575427477
1202	19	0.24962142190721437
1202	20	0.3983869934819779
1202	21	0.38409292539349593
1202	22	0.3707265463539642
1202	23	0.6407903516217459
1202	24	0.38334340170146675
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2211	8	0.3591160155519617
2211	9	0.47930303451363443
2211	10	0.37092128927449863
2211	11	0.25737950396650433
2211	12	0.21804126028100512
2211	13	0.1622762777454838
2211	14	0.40551050471089667
2211	15	0.2553286842806344
2211	16	0.2784399611608208
2211	17	0.30348093724862224
2211	18	0.24601318310376996
2211	19	0.2845476872207158
2211	20	0.27727900254691207
2211	21	0.49370470980575476
2211	22	0.4449694931139092
2211	23	0.625930923763498
2211	24	0.5873840296779491
2211	25	0.624415242221616
2212	1	-0.15363334218818636
2212	2	-0.22492916729251888
2212	3	-0.10356051503476849
2212	4	-0.34536131592325603
2212	5	0.022513218759009135
2212	6	-0.3973326036297067
2212	7	-0.41250408656100035
2212	8	-0.1481944730613165
2212	9	-0.01617375843456159
2212	10	-0.29254676019269965
2212	11	-0.28227948839482764
2212	12	-0.5376439328239968
2212	13	-0.5249720204832471
2212	14	-0.21755458432767938
2212	15	-0.4942636924968653
2212	16	-0.2994940350614286
2212	17	-0.3475111686710477
2212	18	-0.2584120161786956
2212	19	-0.3337536302413042
2212	20	-0.3514913266403923
2212	21	-0.29986271359968697
2212	22	-0.3222160207437952
2212	23	0.20451236524989014
2212	24	0.02469543897026938
2212	25	-0.6060455724902454
2213	1	0.016961400793579884
2213	2	-1.0386154269678585
2213	3	-0.06001108379133768
2213	4	0.3098161017500374
2213	5	0.1704721688205737
2213	6	0.15583370236893757
2213	7	0.17512433097986957
2213	8	0.22941473468064544
2213	9	0.24699978205747186
2213	10	-0.17590235350568406
2213	11	0.12140629931259639
2213	12	0.1392934955002758
2213	13	-0.2851306017054043
2213	14	0.13143751760612332
2213	15	0.04285042089377811
2213	16	0.06111974865610392
2213	17	0.15708048045532053
2213	18	0.23170694791511437
2213	19	0.11611280988076089
2213	20	0.2871249479406828
2213	21	-0.39664044065852266
2213	22	-0.7468973367312515
2213	23	-0.031513862078043076
2213	24	0.18667817621516036
2213	25	-0.4465457739571477
2214	1	-0.1428921008596582
2214	2	-0.36084238474785124
2214	3	-0.2373191865070794
2214	4	-0.3709140011730341
2214	5	-0.15883833193277042
2214	6	-0.057246179570483946
2214	7	-0.40626601589092104
2214	8	-0.0716169554277073
2214	9	-1.5303167798208506E-4
2214	10	-1.8486170330291856
2214	11	-0.7726061340368062
2214	12	-0.32649237099010264
2214	13	-0.17299532445952792
2214	14	-0.6726046269583819
2214	15	-0.4141985111226063
2214	16	-0.5902305773239576
2214	17	-0.41343948853695167
2214	18	-0.32857373429286696
2214	19	-1.3369130860216387
2214	20	-2.343935276956252
2214	21	-1.7882113310085188
2214	22	-1.9278090598283981
2214	23	-2.4357296245381193
2214	24	-1.4470387101596742
2214	25	-1.7718874804079263
2215	1	-0.20474778502860502
2215	2	-0.3970340669208916
2215	3	-0.7548692400430497
2215	4	-0.28247580598503674
2215	5	-0.2506873663361304
2215	6	-0.19580182579645822
2215	7	-0.28706221080864225
2215	8	-0.23720445100969556
2215	9	-0.2795098950676902
2215	10	-0.2826724739473079
2215	11	-0.585542072137784
2215	12	-0.416181693422196
2215	13	-0.30247306609908264
2215	14	-0.1835717699046242
2215	15	-0.6043041342189639
2215	16	-0.49543682076190265
2215	17	-0.3265314865982276
2215	18	-0.3474231559224421
2215	19	-2.239888734300692
2215	20	-2.4128473142339217
2215	21	-3.3525369791576267
2215	22	-2.2683448061354525
2215	23	-3.3261366960944088
2215	24	-2.2662546810319455
2215	25	-1.546991888549556
2216	1	-1.3789706292184292
2216	2	-1.0749092700929275
2216	3	-0.7582628507836553
2216	4	-0.770712600713864
2216	5	-1.7506720601021186
2216	6	-1.5572024190952476
2216	7	-0.9778558754854956
2216	8	-4.96532054515032
2216	9	-3.9955617613349403
2216	10	-1.9356699447743537
2216	11	-1.0785558696144406
2216	12	-1.0264295815263367
2216	13	-1.8509377050541715
2216	14	-3.327755036546513
2216	15	-1.9854497693912307
2216	16	-2.9693805083981104
2216	17	-2.6723576223572323
2216	18	-2.5399420518327602
2216	19	-1.6690303461200173
2216	20	-0.888250196179122
2216	21	-0.6796035029661098
2216	22	-0.7100229524776296
2216	23	-0.8660560832619524
2216	24	-0.5100829888184464
2216	25	-0.31057762897039964
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	7.0
7	92.0
8	599.0
9	1464.0
10	2656.0
11	4567.0
12	7271.0
13	10513.0
14	14267.0
15	19948.0
16	23794.0
17	26199.0
18	26903.0
19	27626.0
20	28251.0
21	29986.0
22	31710.0
23	32935.0
24	35277.0
25	37671.0
26	39855.0
27	44740.0
28	50375.0
29	59220.0
30	70529.0
31	86344.0
32	116108.0
33	164427.0
34	238796.0
35	376968.0
36	660196.0
37	2132166.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.495954018652085	19.777706818907532	11.597563206739931	24.128775955700455
2	13.223128477275644	22.310052841524282	38.13490210513998	26.3319165760601
3	20.258719224628567	28.267666936776557	27.7860366061775	23.687577232417368
4	12.854145509307116	18.490954667784102	35.06692448251437	33.58797534039441
5	12.086500956109843	39.546225610109424	35.22085966943482	13.146413764345906
6	32.2228162733243	35.073186060667894	17.853858733032148	14.850138932975659
7	24.83302475734431	33.50692200051756	23.058498873426895	18.601554368711234
8	26.014597166993607	36.57752688802341	21.448279434598337	15.959596510384646
9	25.990471696248456	17.448669325055384	20.904377753235153	35.65648122546101
10	15.677505543640263	31.60049892762369	32.63952251990258	20.082473008833475
11	30.34951542779038	27.33241271883906	20.443867053451985	21.87420479991857
12	22.29222648937739	30.331134825719293	29.645135924026413	17.73150276087691
13	27.800045031832415	23.09826277551041	24.19683954694614	24.90485264571104
14	20.745999815514168	25.543108568094585	26.68385353642527	27.027038079965976
15	28.44376354410294	26.927778265000697	22.094615257731583	22.53384293316478
16	19.981655636722532	30.048653232006895	29.403396956208	20.566294175062573
17	23.745873425199086	30.246288070705578	27.748054574164744	18.259783929930588
18	23.979971590521814	27.718258133644515	28.757258003684328	19.54451227214935
19	22.186140571817795	30.52132746646152	29.272985163709038	18.01954679801165
20	23.082106705750924	28.185515119968933	27.628014616155518	21.10436355812462
21	20.179741441747304	29.97574325094104	29.353759000204434	20.490756307107226
22	21.23963168639407	29.395735206038324	24.864829343208633	24.49980376435897
23	18.831319293150457	30.357042561459814	27.9446739451501	22.866964200239625
24	20.802359252009875	33.00501318332029	25.900970033364423	20.291657531305415
25	22.010851867994138	27.342922763100187	24.044871208074138	26.60135416083153
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9451.0
1	9451.0
2	11402.5
3	13354.0
4	13354.0
5	13354.0
6	16268.0
7	19182.0
8	19182.0
9	19182.0
10	21228.0
11	23274.0
12	23274.0
13	23274.0
14	30522.5
15	37771.0
16	37771.0
17	37771.0
18	53855.5
19	69940.0
20	69940.0
21	69940.0
22	96380.0
23	122820.0
24	122820.0
25	122820.0
26	158698.0
27	194576.0
28	194576.0
29	194576.0
30	232675.0
31	270774.0
32	270774.0
33	270774.0
34	306220.0
35	341666.0
36	341666.0
37	341666.0
38	373621.5
39	405577.0
40	405577.0
41	405577.0
42	440949.0
43	476321.0
44	476321.0
45	476321.0
46	695577.0
47	914833.0
48	914833.0
49	914833.0
50	797100.5
51	679368.0
52	679368.0
53	679368.0
54	514596.5
55	349825.0
56	349825.0
57	349825.0
58	279516.0
59	209207.0
60	209207.0
61	209207.0
62	172423.5
63	135640.0
64	135640.0
65	135640.0
66	104318.5
67	72997.0
68	72997.0
69	72997.0
70	53395.5
71	33794.0
72	33794.0
73	33794.0
74	23841.0
75	13888.0
76	13888.0
77	13888.0
78	9398.5
79	4909.0
80	4909.0
81	4909.0
82	3301.0
83	1693.0
84	1693.0
85	1693.0
86	1063.5
87	434.0
88	434.0
89	434.0
90	281.0
91	128.0
92	128.0
93	128.0
94	74.5
95	21.0
96	21.0
97	21.0
98	19.0
99	17.0
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03214842347766423
2	0.012836649657159215
3	0.017766831914864615
4	0.020515919717548268
5	0.01851658313377834
6	0.009474129039000696
7	0.001340464300482113
8	0.0013177445665756363
9	0.001067827493604395
10	4.5439467812952974E-4
11	0.0011814261631367775
12	0.0032034824808131845
13	0.002930845673935467
14	8.633498884461065E-4
15	0.002226533922834696
16	0.0024537312618994607
17	0.0024991707297124135
18	0.002544610197525367
19	5.225538798489592E-4
20	6.588722832878181E-4
21	0.08908407664729431
22	0.08374493917927234
23	0.08517628241538035
24	0.0014767827039209717
25	0.22624311024069285
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4401460.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.51164875266364
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.26595567555816	51.93340902229331
2	9.012548872930848	10.727032857841504
3	1.8927961248507006	3.379302544275533
4	0.6322429741442991	1.5050328721445971
5	0.3194063092279553	0.9504198042079373
6	0.18831937637488116	0.6724317948085554
7	0.10927256527375928	0.45520933660121315
8	0.08585331917404845	0.4087418059949035
9	0.06407247474716482	0.34317527506804657
>10	0.3757964920121527	4.1596343388459935
>50	0.029589458102874767	1.2118220492410148
>100	0.018259309451030766	2.0726378606551052
>500	0.0021301819135054125	0.8741642175698285
>1k	0.002323834786917004	2.7984502716832957
>5k	5.809586967292506E-4	2.7189766073275146
>10k+	8.520727552029009E-4	15.789559341441613
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	122781	2.7895516487710896	No Hit
CCCATGTACTCTGCGTTGATACCAC	118917	2.7017625969564643	No Hit
GAGTACATGGGAAGCAGTGGTATCA	57640	1.3095654623693047	No Hit
CATGTACTCTGCGTTGATACCACTG	46293	1.0517646417325162	No Hit
GCGTTGATACCACTGCTTCCCATGT	38611	0.8772316458629636	No Hit
GCTTCCCATGTACTCTGCGTTGATA	31502	0.7157170575218222	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	31484	0.7153081023115058	No Hit
GTATCAACGCAGAGTACATGGGAAG	30943	0.703016726268102	No Hit
ACTCTGCGTTGATACCACTGCTTCC	25916	0.5888046239202446	No Hit
TATCAACGCAGAGTACATGGGAAGC	23943	0.5439785889227665	No Hit
GGTATCAACGCAGAGTACATGGGAA	20260	0.4603018089452136	No Hit
CAGTGGTATCAACGCAGAGTACATG	16453	0.37380778196325765	No Hit
GTGGTATCAACGCAGAGTACATGGG	15353	0.3488160746661335	No Hit
GCAGTGGTATCAACGCAGAGTACAT	13105	0.29774211284437435	No Hit
ATACCACTGCTTCCCATGTACTCTG	13099	0.29760579444093554	No Hit
GTACTCTGCGTTGATACCACTGCTT	12072	0.27427262771898414	No Hit
CATGGGAAGCAGTGGTATCAACGCA	11738	0.266684236594221	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	11384	0.25864145079132833	No Hit
GTACATGGTAAGCAGTGGTATCAAC	11265	0.25593780245645764	No Hit
ACATGGGAAGCAGTGGTATCAACGC	11236	0.25527893017316977	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	10940	0.2485538889368528	No Hit
TACCACTGCTTCCCATGTACTCTGC	10102	0.22951475192322549	No Hit
CTTCCCATGTACTCTGCGTTGATAC	9580	0.21765505082404474	No Hit
GTTGATACCACTGCTTCCCATGTAC	9480	0.21538307743339707	No Hit
GATACCACTGCTTCCCATGTACTCT	9364	0.21274758830024582	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	9236	0.20983946236021686	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	9171	0.20836267965629587	No Hit
CCACTGCTTCCCATGTACTCTGCGT	8839	0.20081972799934567	No Hit
CCATGTACTCTGCGTTGATACCACT	8606	0.19552602999913668	No Hit
ACCATGTACTCTGCGTTGATACCAC	7802	0.17725936393832956	No Hit
GTACATGGGTAAGCAGTGGTATCAA	7417	0.16851226638433608	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	7297	0.16578589831555893	No Hit
TTCCCATGTACTCTGCGTTGATACC	7066	0.16053763978316288	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	6875	0.15619817060702584	No Hit
TGATACCACTGCTTCCCATGTACTC	6543	0.14865521895007566	No Hit
CCCCATGTACTCTGCGTTGATACCA	5445	0.12370895112076448	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	5243	0.11911956487165622	No Hit
ATCAACGCAGAGTACATGGGAAGCA	4700	0.1067827493604395	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.271973390647649E-5	0.0
4	0.0	0.0	0.0	6.815920171942946E-5	0.0
5	0.0	0.0	0.0	6.815920171942946E-5	0.0
6	0.0	0.0	0.0	6.815920171942946E-5	0.0
7	0.0	0.0	0.0	6.815920171942946E-5	0.0
8	0.0	0.0	0.0	6.815920171942946E-5	0.0
9	0.0	0.0	0.0	9.087893562590595E-5	0.0
10	0.0	0.0	0.0	1.3631840343885892E-4	0.0
11	0.0	0.0	0.0	1.590381373453354E-4	0.0
12	0.0	0.0	0.0	2.044776051582884E-4	0.0
13	0.0	0.0	0.0	2.044776051582884E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCGTC	50	0.0015050832	13.294393	7
ACCGTCC	70	1.096082E-4	12.209136	8
CCGTCCA	100	1.941593E-6	11.395324	9
GGACCGC	60	0.0058950405	11.079165	6
GTACAAA	955	0.0	11.044005	1
CGCAAAA	295	0.0	10.304486	2
TAGGCCG	85	6.611928E-4	10.056526	5
CAGGACG	95	1.6480396E-4	9.998397	4
GGTATCG	135	6.407445E-7	9.856769	17
TAGACAG	525	0.0	9.769196	5
GTTCTAG	265	0.0	9.681105	1
GTCCTAG	315	0.0	9.652648	1
CTACACT	445	0.0	9.605201	4
CCGAGAC	100	2.760596E-4	9.498909	3
TATTCCG	150	2.660745E-7	9.497829	5
GAACAAA	1495	0.0	9.406488	1
GTATTAG	475	0.0	9.401806	1
TATACTG	760	0.0	9.247887	5
GACTGTC	280	0.0	9.156853	7
CTAGACA	415	0.0	9.155158	4
>>END_MODULE