FastQCFastQC Report
Wed 25 May 2016
SRR1294769_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294769_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4401460
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1312982.98305562245255No Hit
GTACATGGGAAGCAGTGGTATCAAC1202802.732729594270992No Hit
GAGTACATGGGAAGCAGTGGTATCA611701.3897661230591667No Hit
CATGTACTCTGCGTTGATACCACTG529821.2037369418329371No Hit
GCGTTGATACCACTGCTTCCCATGT413460.9393701180971767No Hit
ACGCAGAGTACATGGGAAGCAGTGG384830.8743235199229347No Hit
GCTTCCCATGTACTCTGCGTTGATA348010.7906694596792883No Hit
GTATCAACGCAGAGTACATGGGAAG329670.7490014676948104No Hit
ACTCTGCGTTGATACCACTGCTTCC298670.6785702925847332No Hit
TATCAACGCAGAGTACATGGGAAGC279910.6359480717761833No Hit
GGTATCAACGCAGAGTACATGGGAA222010.5044008124576845No Hit
CAGTGGTATCAACGCAGAGTACATG199260.45271341782045044No Hit
GTGGTATCAACGCAGAGTACATGGG165160.3752391251993657No Hit
GCAGTGGTATCAACGCAGAGTACAT151820.34493100016812606No Hit
CATGGGAAGCAGTGGTATCAACGCA150760.34252270837403953No Hit
ATACCACTGCTTCCCATGTACTCTG147150.33432088443380154No Hit
ACATGGGAAGCAGTGGTATCAACGC142750.3243242015149519No Hit
GGGAAGCAGTGGTATCAACGCAGAG126670.28779086939333764No Hit
CTGCTTCCCATGTACTCTGCGTTGA123900.2814975031012437No Hit
GTACTCTGCGTTGATACCACTGCTT119950.27252320820818543No Hit
CTTCCCATGTACTCTGCGTTGATAC115480.2623674871519905No Hit
TACCACTGCTTCCCATGTACTCTGC113320.25746002462819156No Hit
AAGCAGTGGTATCAACGCAGAGTAC112720.25609684059380294No Hit
GATACCACTGCTTCCCATGTACTCT109510.24880380600982405No Hit
GTACATGGTAAGCAGTGGTATCAAC107180.243510108009615No Hit
GCAGAGTACATGGGAAGCAGTGGTA106820.24269219758898183No Hit
GTATCAACGCAGAGTACTTTTTTTT106280.2414653319580321No Hit
CCACTGCTTCCCATGTACTCTGCGT104640.23773929559736998No Hit
GTTGATACCACTGCTTCCCATGTAC101220.22996914660135503No Hit
CCATGTACTCTGCGTTGATACCACT100970.22940115325369312No Hit
ATGGGAAGCAGTGGTATCAACGCAG89920.2042958472870366No Hit
ACCATGTACTCTGCGTTGATACCAC87710.19927478609370525No Hit
GTACATGGGTAAGCAGTGGTATCAA81620.18543846814466108No Hit
TATCAACGCAGAGTACTTTTTTTTT80150.18209866726040905No Hit
GGTATCAACGCAGAGTACTTTTTTT78930.17932685972381893No Hit
TTCCCATGTACTCTGCGTTGATACC73880.1678533941010483No Hit
TGATACCACTGCTTCCCATGTACTC67360.1530401275940256No Hit
CCCCATGTACTCTGCGTTGATACCA63770.14488374312160054No Hit
GGTAAGCAGTGGTATCAACGCAGAG61170.13897661230591668No Hit
ACGCAGAGTACTTTTTTTTTTTTTT58120.13204709346444135No Hit
ATCAACGCAGAGTACATGGGAAGCA58100.1320016539966284No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA51840.1177791005711741No Hit
GTACTTTTTTTTTTTTTTTTTTTTT51820.11773366110336114No Hit
GCTTACCATGTACTCTGCGTTGATA45490.10335206954056154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCGA759.757969E-713.9239710
GTATTAG4600.010.9682011
CGTAGTA803.7158225E-410.705372
ATTAACG803.7162137E-410.7052483
AACCGTG1002.4177785E-510.4434527
CTGTGCG1606.2482286E-910.0873119
ATACCGT951.652782E-49.9955516
TTAGACT2700.09.8682134
CTAGGAC4300.09.7370733
GTGTACG900.00109358759.519571
TTAGACA3000.09.5157774
TCTACGC1106.719252E-59.5157763
CTACGCA1401.0361891E-69.5157764
TATACCG800.0044568019.5157765
GATCCGG800.00453483979.4957736
GCCGCTC900.00112077339.4940478
GACTGCG900.00112077339.4940477
CCGCTCA1201.7149909E-59.4939399
GTCTTAG2850.09.352561
ATTAGAC3100.09.2088163
Produced by FastQC (version 0.11.5)