FastQCFastQC Report
Wed 25 May 2016
SRR1294768_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294768_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4100806
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC63230.15418920085466126No Hit
GTACATGGGAAGCAGTGGTATCAAC63120.15392096090378332No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACCGC751.4850797E-512.6624316
CTACGAC1158.845236E-710.7375043
GTCCTAG4700.010.5122511
GTCGTAC900.001112779.5014581
ACCGACC1002.768282E-49.4962448
ATCGTGC1502.666984E-79.4962448
TACGACA1356.8714508E-69.1463164
GTCGTAT850.0074297368.9425491
TAGACAG5850.08.9298345
GTATAGA6200.08.8884611
GTCCCGA2151.0404619E-98.8385651
GCTAGAC1951.6556442E-88.7679023
TTACACC4250.08.7159014
TATACTG8750.08.6839755
CTAAGAC3400.08.6604523
TAGGACT3950.08.6564944
AGCCGTC1107.176791E-48.632957
CTAGCCG1651.0573149E-68.63284410
GTCTAGA4200.08.5965571
GTATTAT7650.08.5699421
Produced by FastQC (version 0.11.5)