##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294768_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4100806
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.254052008312513	33.0	33.0	33.0	27.0	33.0
2	31.307682197109543	33.0	33.0	33.0	27.0	33.0
3	31.18968246730033	33.0	33.0	33.0	27.0	33.0
4	34.7522757721287	37.0	37.0	37.0	27.0	37.0
5	34.8670337002043	37.0	37.0	37.0	33.0	37.0
6	35.136739216632044	37.0	37.0	37.0	33.0	37.0
7	35.12090184222321	37.0	37.0	37.0	33.0	37.0
8	35.06753111461503	37.0	37.0	37.0	33.0	37.0
9	34.7834381826402	37.0	37.0	37.0	27.0	37.0
10	34.85634823983383	37.0	37.0	37.0	33.0	37.0
11	35.09103381140196	37.0	37.0	37.0	33.0	37.0
12	35.01129460891347	37.0	37.0	37.0	33.0	37.0
13	34.953313568113195	37.0	37.0	37.0	33.0	37.0
14	36.126445142735356	40.0	37.0	40.0	27.0	40.0
15	36.439066612758566	40.0	37.0	40.0	33.0	40.0
16	36.46088963974399	40.0	37.0	40.0	33.0	40.0
17	36.41062952014799	40.0	37.0	40.0	33.0	40.0
18	36.408256572000724	40.0	37.0	40.0	33.0	40.0
19	36.48247222619163	40.0	37.0	40.0	33.0	40.0
20	36.362329259174906	40.0	37.0	40.0	27.0	40.0
21	36.30364177188582	40.0	37.0	40.0	27.0	40.0
22	36.16372537496287	40.0	37.0	40.0	27.0	40.0
23	35.755421251334496	37.0	37.0	40.0	27.0	40.0
24	36.05953024844384	37.0	37.0	40.0	27.0	40.0
25	34.9524581265244	37.0	33.0	40.0	27.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.47476376137436205
1101	2	0.6724417161631813
1101	3	0.43380991341526354
1101	4	0.5450938641478444
1101	5	0.5039972098944503
1101	6	0.47659693954017257
1101	7	0.4986576615356668
1101	8	0.6068083739154915
1101	9	0.7878937553118206
1101	10	0.7787969727479265
1101	11	0.5454101500173181
1101	12	0.5631170580912652
1101	13	0.6413834184827465
1101	14	1.2579376942461167
1101	15	1.056890043413901
1101	16	0.9516082676375461
1101	17	0.9687465436161133
1101	18	1.0150805125273266
1101	19	0.8889943201201334
1101	20	1.0465100172186297
1101	21	1.0279596648281313
1101	22	1.1279255124809353
1101	23	1.4446410628853172
1101	24	1.2373841607580118
1101	25	1.0505006603146825
1102	1	0.15424597693724706
1102	2	0.45565214466227744
1102	3	0.15610219617528998
1102	4	0.12240752075197747
1102	5	0.14895309028700154
1102	6	0.24977200301011493
1102	7	0.28608825109030533
1102	8	0.4466494763766349
1102	9	0.6055201377670301
1102	10	0.6144396735697057
1102	11	0.36198158934889335
1102	12	0.4707326744177607
1102	13	0.3878389650117313
1102	14	1.0342381033111891
1102	15	0.7785823580957114
1102	16	0.5583534176489948
1102	17	0.7141504348453296
1102	18	0.6684838286862629
1102	19	0.6661913486446949
1102	20	0.7432716302608853
1102	21	0.7963038987920683
1102	22	0.8340705449676236
1102	23	1.153087063964918
1102	24	0.9234277967564566
1102	25	0.9289766822151719
1103	1	0.3651127827169809
1103	2	0.5744365359786698
1103	3	0.2793115514562814
1103	4	0.15931436819005285
1103	5	0.27006668078475826
1103	6	0.21884108779089928
1103	7	0.28271192525065203
1103	8	0.470214665788113
1103	9	0.6788694635802415
1103	10	0.689429173236455
1103	11	0.47790907615480904
1103	12	0.4159786612557923
1103	13	0.42289086499798145
1103	14	1.0407001173928236
1103	15	0.7698273618990825
1103	16	0.5254435275122731
1103	17	0.5949045520659695
1103	18	0.7115701040749087
1103	19	0.7560244972593679
1103	20	0.8621358386433045
1103	21	0.8875753823285635
1103	22	0.7912471775768779
1103	23	1.1930206524454476
1103	24	0.9028599619876658
1103	25	0.7253014347779114
1104	1	0.3296001253494296
1104	2	0.5901771749603313
1104	3	0.3510440774588801
1104	4	0.27534617572769093
1104	5	0.2592532737423099
1104	6	0.16741689351785283
1104	7	0.1886112631057557
1104	8	0.44300672452104806
1104	9	0.5153342534196526
1104	10	0.6618121031423385
1104	11	0.4096782950481028
1104	12	0.3908331902026987
1104	13	0.48276573515482823
1104	14	0.9087228054824408
1104	15	0.6788584142410272
1104	16	0.5780235329367471
1104	17	0.6674552210645501
1104	18	0.5343875968447094
1104	19	0.6068891427212719
1104	20	0.7590079996008328
1104	21	0.7914866057587702
1104	22	0.7849535075247616
1104	23	1.2720508236397663
1104	24	1.0033797874940618
1104	25	0.8284764269711218
1105	1	0.12843067447523993
1105	2	0.26102381889622706
1105	3	0.21837266024216007
1105	4	0.13750878675144662
1105	5	0.16854248293028462
1105	6	0.16785571570461144
1105	7	0.17000692722455568
1105	8	0.2742424422929517
1105	9	-1.5862425905226445
1105	10	-0.21703982497566443
1105	11	0.09689797037306391
1105	12	0.126981216295043
1105	13	0.1974309698133183
1105	14	-1.7793154090447203
1105	15	-0.2760769546474364
1105	16	-0.1613655859023453
1105	17	0.16969130116464015
1105	18	0.03242288364328516
1105	19	0.10435896944704837
1105	20	0.2869076349747388
1105	21	0.38228318896297964
1105	22	0.3996700153575148
1105	23	0.6694361839068534
1105	24	0.5231450455755038
1105	25	0.25123964878951455
1106	1	0.0469706146558444
1106	2	0.10254037983905917
1106	3	0.14558828977283866
1106	4	-1.1526891046039722
1106	5	-0.29644421632918494
1106	6	-0.03780818925471863
1106	7	0.11353873512475587
1106	8	0.21549553578560676
1106	9	0.5071005702588707
1106	10	0.4696408823391707
1106	11	0.3151057816860572
1106	12	0.15430710295661498
1106	13	0.17170480870718308
1106	14	0.6558849383347933
1106	15	0.3978363657178221
1106	16	0.2740620966315248
1106	17	0.15208552160616762
1106	18	0.21633459347346218
1106	19	0.20698667222573164
1106	20	0.42892197024523426
1106	21	0.6645858552616204
1106	22	0.5346823775968517
1106	23	0.871949490094039
1106	24	0.4825898953072141
1106	25	0.5151421613080345
1107	1	0.42712041568124803
1107	2	0.3755618477509586
1107	3	0.36968519441803593
1107	4	0.5422367413709424
1107	5	0.4384667018783688
1107	6	0.3363520783441345
1107	7	0.29011277943452285
1107	8	0.40736131185433777
1107	9	0.5640734708563286
1107	10	0.5531083204436342
1107	11	0.2550691556592142
1107	12	0.310362910522727
1107	13	0.35630535645343286
1107	14	0.8386620581735258
1107	15	0.5844786141510809
1107	16	0.6672302578778826
1107	17	0.6263777817122929
1107	18	0.7605429197893514
1107	19	0.6152338501700854
1107	20	0.6914163583554753
1107	21	0.721154729569875
1107	22	0.6829633669805872
1107	23	0.9631980980592232
1107	24	0.8725367962687685
1107	25	0.885017295582351
1108	1	0.47289279671513995
1108	2	0.39648712102619754
1108	3	0.5516134934829928
1108	4	0.6033043081025227
1108	5	0.5722642755409666
1108	6	0.415203018607464
1108	7	0.43278118999881343
1108	8	0.49797138824412457
1108	9	0.2339269518691225
1108	10	0.5211623021153571
1108	11	0.28660332674508027
1108	12	0.31363818072978944
1108	13	0.38721443737455985
1108	14	-1.0226502634906893
1108	15	-0.031806821488416404
1108	16	0.3326116302970945
1108	17	0.4562545321723164
1108	18	0.5818693219786084
1108	19	0.5807077235735818
1108	20	0.7024406616188728
1108	21	0.7296415918769341
1108	22	0.8805594962469954
1108	23	1.0653324686920271
1108	24	0.9239356130327323
1108	25	0.9997277861665452
1109	1	0.390599619400664
1109	2	0.24163514197224245
1109	3	0.39957587939354156
1109	4	0.5031563576605365
1109	5	0.5216056079164275
1109	6	0.34525281805903063
1109	7	0.2724789029698442
1109	8	0.36644298839696177
1109	9	0.5407344212906295
1109	10	0.4131023955747537
1109	11	0.27751788333815597
1109	12	0.34572762586870454
1109	13	0.34053733315870005
1109	14	0.7038405006981847
1109	15	0.4690686080520621
1109	16	0.41765940380089717
1109	17	0.4358071502387375
1109	18	0.4753011290979643
1109	19	0.4553867765512152
1109	20	0.6522542238196962
1109	21	0.6398812482062368
1109	22	0.7424040829868161
1109	23	1.0670582214481854
1109	24	0.813373622937398
1109	25	0.6198991852280358
1110	1	0.4321447463585848
1110	2	0.25307944802637294
1110	3	0.3936484730524725
1110	4	0.6445472864302388
1110	5	0.506650863356974
1110	6	0.3520891455682147
1110	7	0.28284946647003295
1110	8	0.28018881116480543
1110	9	0.4372267337746507
1110	10	0.42341570232439096
1110	11	0.2874660652649794
1110	12	0.2889763815100821
1110	13	0.18560256516062879
1110	14	0.6601159048116187
1110	15	0.48305688912302003
1110	16	0.4294499156767273
1110	17	0.4285593879996128
1110	18	0.4956733712727939
1110	19	0.46869135585855304
1110	20	0.5924564253441318
1110	21	0.6422273233321363
1110	22	0.7357111591263461
1110	23	0.8677161314968274
1110	24	0.7250143201218648
1110	25	0.6874541549212267
1111	1	0.1853238420241432
1111	2	0.13536744733821493
1111	3	0.3203188318604653
1111	4	0.5507015442064969
1111	5	0.42725963099169917
1111	6	0.30667105943798134
1111	7	0.28534529256291563
1111	8	0.3766500528972827
1111	9	0.39938577625326843
1111	10	0.20951434338178387
1111	11	0.10238337749780868
1111	12	0.21046042682343824
1111	13	0.16163464713174847
1111	14	-1.8160132285294281
1111	15	-0.49919248514001424
1111	16	0.0043055674737289
1111	17	0.09458425884448474
1111	18	0.19326325759841012
1111	19	0.16234151513451422
1111	20	0.23819071740535946
1111	21	0.32487171761707856
1111	22	0.1368296377630358
1111	23	-2.1319934126047073
1111	24	-0.4728036020124904
1111	25	-0.0995176691809121
1112	1	-0.17559460508430647
1112	2	-0.27976482012380544
1112	3	0.06442812774199069
1112	4	0.13421866002727256
1112	5	-0.7779045114901777
1112	6	-0.9561406269357988
1112	7	-1.1476291335950037
1112	8	-0.8587428971035891
1112	9	-0.7225238479534966
1112	10	-0.947902281114466
1112	11	-0.9297586694744382
1112	12	-0.8340472173199771
1112	13	-0.9065746291075385
1112	14	-0.6057809847365334
1112	15	-0.9777021042460561
1112	16	-0.8320779734901933
1112	17	-0.9671709522783019
1112	18	-1.179957072436011
1112	19	-0.9116958919283746
1112	20	-0.9701937014191557
1112	21	-1.8336671098170925
1112	22	-1.8775164925585415
1112	23	-1.4880145206330155
1112	24	-1.8347566449717334
1112	25	-1.9258952772031819
1113	1	0.21335592955756155
1113	2	-0.03688449733669685
1113	3	0.08119613693998318
1113	4	0.26221494156231273
1113	5	0.24152926926785767
1113	6	0.034678828601016676
1113	7	0.0744183970810468
1113	8	0.05880895199035052
1113	9	0.2663229649224945
1113	10	0.01693271789740436
1113	11	-0.07726977578421668
1113	12	-0.03365468648745207
1113	13	-0.026705568459348683
1113	14	0.15270428737288455
1113	15	-0.024353256588661054
1113	16	0.07346590023804112
1113	17	0.17095109430925248
1113	18	0.20213072089038775
1113	19	0.05809676742568115
1113	20	-0.6012905542705766
1113	21	-0.4666968013782693
1113	22	0.011803809428478473
1113	23	-0.12771067737613606
1113	24	0.08764264803122046
1113	25	-1.0546614018759328
1114	1	-0.7708556365162735
1114	2	-0.5443362353077568
1114	3	-0.3300248478374286
1114	4	-1.3852823431206076
1114	5	-0.6254294410994419
1114	6	-0.4522927774207659
1114	7	-0.29738324522342197
1114	8	-0.3030313436513552
1114	9	-0.28175185004923975
1114	10	-0.38941162465960133
1114	11	-0.40166786648757125
1114	12	-0.37738970180419784
1114	13	-0.4396524188596658
1114	14	-0.34597100778124457
1114	15	-1.1973368604449135
1114	16	-0.6438130998527498
1114	17	-0.6210263626744066
1114	18	-0.616549819953697
1114	19	-0.651020140845354
1114	20	-1.7956176432152802
1114	21	-6.558994464019875
1114	22	-7.5473150323500775
1114	23	-7.616669088470097
1114	24	-5.670079696926699
1114	25	-7.218013111937232
1115	1	-1.0296239355402967
1115	2	-1.060905284706589
1115	3	-0.8114597079048309
1115	4	-0.5421244778796321
1115	5	-0.6308347690639664
1115	6	-1.011914433959113
1115	7	-0.9745956879277031
1115	8	-0.8303781400211676
1115	9	-2.78210868681154
1115	10	-1.5617517296817311
1115	11	-1.153360276021651
1115	12	-1.1123469580819716
1115	13	-1.4807869098916626
1115	14	-1.142421686059734
1115	15	-2.0583040340752703
1115	16	-1.3118847950306787
1115	17	-2.970378717750691
1115	18	-2.7418394482805155
1115	19	-2.0474463375558116
1115	20	-1.8476535320984624
1115	21	-1.3619674576057932
1115	22	-0.8831824046140184
1115	23	-0.23396434713619385
1115	24	-1.376324877513028
1115	25	-1.0089726082152737
1116	1	-1.5947639193026433
1116	2	-1.061972776955109
1116	3	-0.7428943864146795
1116	4	-0.38074632698880606
1116	5	-0.4324710576513979
1116	6	-0.4927262710131046
1116	7	-0.8576357423137964
1116	8	-1.8896159464672877
1116	9	-0.9980090349587414
1116	10	-2.6026663035634385
1116	11	-1.2409676599771444
1116	12	-0.8660663978967094
1116	13	-0.9595167282168262
1116	14	-1.366126522776753
1116	15	-1.476043187703965
1116	16	-1.04806731191983
1116	17	-0.8753061859248277
1116	18	-1.4414887907731568
1116	19	-1.057269269578832
1116	20	-1.3593920832543134
1116	21	-0.7893245078830802
1116	22	-0.4392051214705077
1116	23	-0.33660147818801534
1116	24	-0.341958536577998
1116	25	-0.304332606586442
1201	1	0.18325323810837446
1201	2	0.35100138905000833
1201	3	0.21015987449633755
1201	4	0.32293202724076053
1201	5	0.2856940538263544
1201	6	0.2088169571063787
1201	7	0.20393848758783406
1201	8	0.4054115668541485
1201	9	0.6728881465816627
1201	10	0.6940659561741711
1201	11	0.3760938112429102
1201	12	0.4236999160974477
1201	13	0.4224365075941847
1201	14	1.0106083236102918
1201	15	0.7673942584273021
1201	16	0.7676444642530811
1201	17	0.6683072226880498
1201	18	0.6294294013546491
1201	19	0.640020811276294
1201	20	0.8404862149850345
1201	21	0.7982658846813564
1201	22	0.8528350588309266
1201	23	0.6346303142992227
1201	24	0.7474083006757581
1201	25	0.5902801970811637
1202	1	-0.40841707559395246
1202	2	0.014789862181025626
1202	3	-0.19715851749144875
1202	4	-0.18252024512893428
1202	5	-0.08613664510044572
1202	6	-0.1144176711243503
1202	7	-0.029442564312624597
1202	8	-0.01466083916042038
1202	9	0.20453932836340272
1202	10	0.36219831428987703
1202	11	0.11571032203350029
1202	12	0.053215027012498695
1202	13	0.2006999506469498
1202	14	0.37287689257384926
1202	15	0.40209453419368657
1202	16	0.3708891662530718
1202	17	0.1513840389800052
1202	18	0.23635322287076121
1202	19	0.241667656079251
1202	20	0.38928598962231575
1202	21	0.3815054087709129
1202	22	0.3799598345752315
1202	23	0.7247590650560767
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2211	4	0.48568202024577545
2211	5	0.4118519996934893
2211	6	0.21930692800222573
2211	7	0.19848801504272018
2211	8	0.2331622834264664
2211	9	0.4985728603774504
2211	10	0.43387471377551634
2211	11	0.24362433269575234
2211	12	0.2631392281559286
2211	13	0.22014624408230787
2211	14	0.5015914615336499
2211	15	0.32211655550227647
2211	16	0.27301841817683226
2211	17	0.4042608156986418
2211	18	0.3239855677777328
2211	19	0.39443384475423926
2211	20	0.37851697266279416
2211	21	0.3293179535473172
2211	22	0.4956414208879636
2211	23	0.6955690724250658
2211	24	0.6399233052954614
2211	25	0.6937393903422304
2212	1	-0.29016661130583543
2212	2	-0.2611443986178834
2212	3	-0.09524725896585196
2212	4	-0.5687074022097178
2212	5	-0.20397837914257622
2212	6	-0.582805290219973
2212	7	-0.5492383412529449
2212	8	-0.31940719338612666
2212	9	-0.2643991656239706
2212	10	-0.6828215763360106
2212	11	-0.5328634612721785
2212	12	-0.7434518965791028
2212	13	-0.7901827567587745
2212	14	-0.5652873127149007
2212	15	-0.6953384224287689
2212	16	-0.5146512638674636
2212	17	-0.5909340162123016
2212	18	-0.5004411179558801
2212	19	-0.6396317504024651
2212	20	-0.729229623366102
2212	21	-0.6064200501755934
2212	22	-0.7274631280016877
2212	23	-0.1982560942650835
2212	24	-0.3643260923309697
2212	25	-1.1561978295677022
2213	1	-0.10438146150717387
2213	2	-1.341043195521955
2213	3	-0.21882823478400937
2213	4	0.20429083079643817
2213	5	0.14488324158348576
2213	6	0.08888999629992611
2213	7	0.06662833288339698
2213	8	0.17584638513918804
2213	9	0.1598677042894181
2213	10	-0.23587361873414636
2213	11	0.0825178351350857
2213	12	0.0965424970742248
2213	13	-0.35147395780341384
2213	14	0.17292722710266162
2213	15	0.02751284722094738
2213	16	0.16337991198903978
2213	17	0.17907898706932457
2213	18	0.19493015115700274
2213	19	0.18557797040497093
2213	20	0.3011147734108235
2213	21	-0.3263604841047396
2213	22	-0.7471936168740712
2213	23	-0.005233334400593037
2213	24	0.17695854905189634
2213	25	-0.43072396689953507
2214	1	-0.19437221492860957
2214	2	-0.5175491054102928
2214	3	-0.4512042192755814
2214	4	-0.47416518651081674
2214	5	-0.22110630815471666
2214	6	-0.17335038880760578
2214	7	-0.487350453027787
2214	8	-0.1502527564911773
2214	9	-0.0457055437901559
2214	10	-2.051089235623216
2214	11	-0.8205196350197639
2214	12	-0.6074817299392308
2214	13	-0.28167164164637626
2214	14	-0.8929431217994406
2214	15	-0.640391369229377
2214	16	-0.7664862701556672
2214	17	-0.6177236212826642
2214	18	-0.5119410880651003
2214	19	-1.503013147781445
2214	20	-2.554364019307336
2214	21	-2.002167477239304
2214	22	-2.197436670315774
2214	23	-2.59508170691349
2214	24	-1.8059760820530926
2214	25	-2.2302105634407567
2215	1	-0.29240865568612406
2215	2	-0.44040102744387255
2215	3	-0.8801432306466097
2215	4	-0.3173657172755995
2215	5	-0.24003298115365368
2215	6	-0.23650785857422107
2215	7	-0.25450090957141924
2215	8	-0.2712459676022334
2215	9	-0.30241822279997166
2215	10	-0.2684684810653337
2215	11	-0.6681091976002094
2215	12	-0.5237671878431911
2215	13	-0.3531847985990595
2215	14	-0.26830861922437066
2215	15	-0.716384246225914
2215	16	-0.49059553674246814
2215	17	-0.271623175319327
2215	18	-0.25545449321714386
2215	19	-2.2903436443233645
2215	20	-2.523233495081648
2215	21	-3.4545587826247584
2215	22	-2.3480090005418432
2215	23	-3.6132294555384448
2215	24	-2.3900071976614896
2215	25	-1.883835586709445
2216	1	-1.5944304875415511
2216	2	-1.2219968855648737
2216	3	-0.8589801745610046
2216	4	-0.8872978084193903
2216	5	-1.972008605054576
2216	6	-1.523098197931425
2216	7	-1.1687416586266792
2216	8	-5.236318740453662
2216	9	-4.122273612480814
2216	10	-2.0627878958892936
2216	11	-1.1186559293695808
2216	12	-1.3020686245925432
2216	13	-2.0254773304956473
2216	14	-3.6588860315789162
2216	15	-2.090294056530098
2216	16	-3.204153923392667
2216	17	-2.9314722979294174
2216	18	-2.7035617443115427
2216	19	-1.7420647503210418
2216	20	-1.0408116931585027
2216	21	-0.8373706395484675
2216	22	-0.8289613146811021
2216	23	-1.1887065012225904
2216	24	-0.742627675081323
2216	25	-0.4778706448903165
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	5.0
7	36.0
8	253.0
9	666.0
10	1603.0
11	3146.0
12	5460.0
13	7844.0
14	10469.0
15	13288.0
16	15197.0
17	16173.0
18	17385.0
19	18379.0
20	19542.0
21	21621.0
22	23461.0
23	25641.0
24	27875.0
25	31049.0
26	34367.0
27	39550.0
28	46075.0
29	55045.0
30	66748.0
31	82288.0
32	110457.0
33	157578.0
34	230278.0
35	364594.0
36	630919.0
37	2023814.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.7762167499653	19.64204476294345	12.975657804706762	24.606080682384494
2	15.5497737435168	19.966299319986714	40.02322805590147	24.460698880595015
3	18.544518033704932	24.58078593460285	29.747991065222024	27.126704966470193
4	12.654455589475214	16.340974525132907	37.70770099836971	33.29686888702217
5	13.24363896590253	37.806588074946774	35.61957139195053	13.330201567200165
6	32.19661765426737	37.51147108652132	17.15581923981966	13.136092019391654
7	27.546239114631938	32.33547185758496	22.415999988294978	17.70228903948812
8	25.185262857567864	37.011774546155074	20.206691629306025	17.59627096697104
9	25.302350942418038	16.55243232935385	20.868975934096458	37.27624079413165
10	14.840522542070461	29.773514868687784	33.970989555937756	21.414973033303998
11	33.68460095391911	24.187938500285437	23.408815960526407	18.718644585269047
12	21.603052132054216	26.096606953866864	31.17956759023981	21.120773323839114
13	27.43448873572289	22.356193700257055	26.560566263329715	23.648751300690343
14	21.04211368517363	21.995566210436497	27.487349361011965	29.47497074337791
15	23.0506182304835	30.27744287854668	23.539310991978024	23.13262789899179
16	22.58664412098292	29.631715085505906	25.580271323680208	22.201369469830965
17	21.577477153430184	29.215809399557397	27.416225179693836	21.790488267318583
18	22.01562455800488	28.908114762173703	27.895122910003213	21.18113776981821
19	22.44797032763239	28.46730565001579	27.959280966936817	21.125443055415
20	22.776235660360474	27.89074671837061	27.8475354584722	21.485482162796714
21	22.767653727965865	27.62577398766891	27.730278033966986	21.876294250398235
22	22.721590934049978	27.456951819325276	27.706731081325785	22.11472616529896
23	22.60748157573752	27.520545023338133	27.756952508135278	22.115020892789072
24	22.486640235703454	27.87897616677551	27.50962873584465	22.124754861676386
25	22.567115898221974	27.440142648778217	27.438065286842132	22.554676166157673
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1981.0
1	1981.0
2	2183.5
3	2386.0
4	2386.0
5	2386.0
6	3432.5
7	4479.0
8	4479.0
9	4479.0
10	7438.5
11	10398.0
12	10398.0
13	10398.0
14	18759.5
15	27121.0
16	27121.0
17	27121.0
18	45598.0
19	64075.0
20	64075.0
21	64075.0
22	94565.5
23	125056.0
24	125056.0
25	125056.0
26	165563.5
27	206071.0
28	206071.0
29	206071.0
30	253329.5
31	300588.0
32	300588.0
33	300588.0
34	342317.5
35	384047.0
36	384047.0
37	384047.0
38	416986.0
39	449925.0
40	449925.0
41	449925.0
42	467362.0
43	484799.0
44	484799.0
45	484799.0
46	491719.0
47	498639.0
48	498639.0
49	498639.0
50	479549.0
51	460459.0
52	460459.0
53	460459.0
54	423164.5
55	385870.0
56	385870.0
57	385870.0
58	339247.0
59	292624.0
60	292624.0
61	292624.0
62	246347.0
63	200070.0
64	200070.0
65	200070.0
66	157125.5
67	114181.0
68	114181.0
69	114181.0
70	84202.0
71	54223.0
72	54223.0
73	54223.0
74	38438.0
75	22653.0
76	22653.0
77	22653.0
78	15231.5
79	7810.0
80	7810.0
81	7810.0
82	5190.0
83	2570.0
84	2570.0
85	2570.0
86	1589.5
87	609.0
88	609.0
89	609.0
90	368.5
91	128.0
92	128.0
93	128.0
94	78.5
95	29.0
96	29.0
97	29.0
98	22.0
99	15.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03167669965367784
2	0.014436186447249637
3	0.02126411246959744
4	0.02248338497358812
5	0.02153235242047539
6	0.0068035405722679885
7	7.315635023944074E-5
8	2.682399508779494E-4
9	6.096362519953395E-4
10	1.7069815055869504E-4
11	9.510325531127296E-4
12	0.001609439705267696
13	0.0024141595579015443
14	6.096362519953395E-4
15	0.0017069815055869506
16	0.00231661775758229
17	0.0019752214564649
18	0.0019996069065447136
19	5.120944516760851E-4
20	5.608653518357123E-4
21	0.08186195591793419
22	0.07774081485444569
23	0.07852114925699973
24	0.0014631270047888148
25	0.2213711158245477
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4100806.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.55075991355747
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.75196407649211	47.85692473679711
2	12.896382708996146	15.875643117495514
3	4.233837619765222	7.817877685414704
4	1.798576201352278	4.42814927822689
5	0.9531047488171702	2.9332160783458567
6	0.5631987483493229	2.0799186565959147
7	0.36476966014585455	1.5716294842771212
8	0.2655651862290723	1.307659121518785
9	0.19639202991348237	1.0879270813926856
>10	0.911239753664061	9.784078309259815
>50	0.044387689397068136	1.856103853686156
>100	0.018786708451592284	1.9502310369906133
>500	9.971492736993506E-4	0.3880636977674527
>1k	7.179472375716737E-4	0.7523872220276979
>5k	7.977191528573932E-5	0.31019064020360226
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCAC	6323	0.15418920085466126	No Hit
GTACATGGGAAGCAGTGGTATCAAC	6312	0.15392096090378332	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.438545007981358E-5	0.0
5	0.0	0.0	0.0	2.438545007981358E-5	0.0
6	0.0	0.0	0.0	2.438545007981358E-5	0.0
7	0.0	0.0	0.0	2.438545007981358E-5	0.0
8	0.0	2.438545007981358E-5	0.0	2.438545007981358E-5	0.0
9	0.0	2.438545007981358E-5	0.0	2.438545007981358E-5	0.0
10	2.438545007981358E-5	2.438545007981358E-5	0.0	2.438545007981358E-5	0.0
11	2.438545007981358E-5	2.438545007981358E-5	0.0	2.438545007981358E-5	0.0
12	2.438545007981358E-5	2.438545007981358E-5	0.0	2.438545007981358E-5	0.0
13	2.438545007981358E-5	2.438545007981358E-5	0.0	2.438545007981358E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAACCGC	75	1.4850797E-5	12.662431	6
CTACGAC	115	8.845236E-7	10.737504	3
GTCCTAG	470	0.0	10.512251	1
GTCGTAC	90	0.00111277	9.501458	1
ACCGACC	100	2.768282E-4	9.496244	8
ATCGTGC	150	2.666984E-7	9.496244	8
TACGACA	135	6.8714508E-6	9.146316	4
GTCGTAT	85	0.007429736	8.942549	1
TAGACAG	585	0.0	8.929834	5
GTATAGA	620	0.0	8.888461	1
GTCCCGA	215	1.0404619E-9	8.838565	1
GCTAGAC	195	1.6556442E-8	8.767902	3
TTACACC	425	0.0	8.715901	4
TATACTG	875	0.0	8.683975	5
CTAAGAC	340	0.0	8.660452	3
TAGGACT	395	0.0	8.656494	4
AGCCGTC	110	7.176791E-4	8.63295	7
CTAGCCG	165	1.0573149E-6	8.632844	10
GTCTAGA	420	0.0	8.596557	1
GTATTAT	765	0.0	8.569942	1
>>END_MODULE