FastQCFastQC Report
Wed 25 May 2016
SRR1294768_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294768_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4100806
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT93590.22822342729697528No Hit
CCCATGTACTCTGCGTTGATACCAC69140.16860100185183108No Hit
GGTATCAACGCAGAGTACTTTTTTT66980.16333374463459135No Hit
TATCAACGCAGAGTACTTTTTTTTT63580.15504269160745474No Hit
GTACATGGGAAGCAGTGGTATCAAC63250.15423797175482087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCT801.9697472E-613.0812234
TACGCTG1103.7447535E-812.1082395
TAGGACG550.003037164712.1082394
GATCGTA700.001499714310.85069811
CTAGGAC3950.010.5974463
GTCGTAC1002.3515862E-510.4697111
GTACCGT1106.082495E-610.3592536
GGGTCGT750.00265846710.1290476
TACCGTG1701.5770638E-910.0533447
TACCCCG951.6217057E-410.0143335
AGGACTG6950.09.582065
TAGACTG4500.09.5136175
TACGACC900.001099839.5136174
CTATACT5750.09.2654354
TCTACGC1451.6752638E-69.1855613
CTAGACT3350.09.0876344
TAGGACT5250.09.0605884
AACCGGT850.0074724188.9363057
TCTAGAC3750.08.8793753
GTATTAA6900.08.8282151
Produced by FastQC (version 0.11.5)