FastQCFastQC Report
Wed 25 May 2016
SRR1294767_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294767_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3369662
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1172703.480171008249492No Hit
CCCATGTACTCTGCGTTGATACCAC1117233.3155550912821528No Hit
GAGTACATGGGAAGCAGTGGTATCA566391.6808510764581137No Hit
CATGTACTCTGCGTTGATACCACTG455981.3531921005726983No Hit
GCGTTGATACCACTGCTTCCCATGT376851.1183614261608434No Hit
GCTTCCCATGTACTCTGCGTTGATA338511.0045814684084042No Hit
ACGCAGAGTACATGGGAAGCAGTGG301340.8942736689911333No Hit
GTATCAACGCAGAGTACATGGGAAG299690.8893770354415369No Hit
ACTCTGCGTTGATACCACTGCTTCC249790.7412909662749558No Hit
TATCAACGCAGAGTACATGGGAAGC237120.7036907559274491No Hit
GGTATCAACGCAGAGTACATGGGAA206120.6116933983289718No Hit
CAGTGGTATCAACGCAGAGTACATG165230.4903459159998837No Hit
GTGGTATCAACGCAGAGTACATGGG158400.4700768207612514No Hit
GCAGTGGTATCAACGCAGAGTACAT131030.3888520569718862No Hit
ATACCACTGCTTCCCATGTACTCTG129720.38496442669917635No Hit
GTACTCTGCGTTGATACCACTGCTT121460.36045158238422725No Hit
CATGGGAAGCAGTGGTATCAACGCA117310.3481358070928182No Hit
ACATGGGAAGCAGTGGTATCAACGC115060.3414585795251868No Hit
CTGCTTCCCATGTACTCTGCGTTGA114340.33932186670354475No Hit
GGGAAGCAGTGGTATCAACGCAGAG109330.32445390665295215No Hit
GTTGATACCACTGCTTCCCATGTAC100330.29774499638242646No Hit
TACCACTGCTTCCCATGTACTCTGC99140.29421348491332366No Hit
GTACATGGTAAGCAGTGGTATCAAC98640.29272965656496114No Hit
CTTCCCATGTACTCTGCGTTGATAC97500.28934652793069454No Hit
GATACCACTGCTTCCCATGTACTCT95440.2832331551354409No Hit
AAGCAGTGGTATCAACGCAGAGTAC94640.2808590297780608No Hit
GCAGAGTACATGGGAAGCAGTGGTA92890.27566563055879195No Hit
CCACTGCTTCCCATGTACTCTGCGT91860.27260894416116516No Hit
ATGGGAAGCAGTGGTATCAACGCAG78310.23239719592054042No Hit
CCATGTACTCTGCGTTGATACCACT77870.23109142697398136No Hit
TTCCCATGTACTCTGCGTTGATACC73200.21723247020027525No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA66730.19803173137246405No Hit
GTACATGGGTAAGCAGTGGTATCAA66530.19743820003311907No Hit
TGATACCACTGCTTCCCATGTACTC66320.19681499212680675No Hit
ACCATGTACTCTGCGTTGATACCAC66030.1959543716847565No Hit
CCCCATGTACTCTGCGTTGATACCA53210.15790901283274109No Hit
GGTAAGCAGTGGTATCAACGCAGAG46080.13674962058509132No Hit
ATCAACGCAGAGTACATGGGAAGCA45470.13493935000008903No Hit
GCTTACCATGTACTCTGCGTTGATA39420.11698502698490235No Hit
GGAAGCAGTGGTATCAACGCAGAGT35430.1051440767649693No Hit
AAAAAGTACTCTGCGTTGATACCAC33740.10012873694750393No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCC550.003079816512.0856118
CGCAAAA3450.011.0152242
GAACAAA16500.010.4815071
GTACAAA8500.010.2850211
GACTCCG951.6526545E-49.9954717
GTCCTAG2500.09.8825641
TGGACTG2950.09.6599385
CTTAGGC1401.0586155E-69.5006313
TCTATAC3200.09.500633
AGAACCG800.0045219269.4989395
ACTGATC2201.6370905E-119.4958378
ACGCAAA4550.09.3980431
GTGTAGG3550.09.3686281
GTCTAGC1356.830169E-69.1505221
GTGTTAG3850.09.1322381
TCTGTCG1252.7702661E-59.1160048
GTCTTAG2304.1836756E-119.0893141
CTAGGAC2106.675691E-109.0482213
CGGCGTT1054.515313E-49.0435219
CTAGTAC950.00182350639.0005983
Produced by FastQC (version 0.11.5)