##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294767_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3369662
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.233001114058325	33.0	33.0	33.0	27.0	33.0
2	31.22569444650532	33.0	33.0	33.0	27.0	33.0
3	31.295790200916294	33.0	33.0	33.0	27.0	33.0
4	34.726920979018075	37.0	37.0	37.0	27.0	37.0
5	35.11327159816029	37.0	37.0	37.0	33.0	37.0
6	35.15419855166483	37.0	37.0	37.0	33.0	37.0
7	35.23092553496463	37.0	37.0	37.0	33.0	37.0
8	35.0852854084475	37.0	37.0	37.0	33.0	37.0
9	34.802245150997344	37.0	37.0	37.0	27.0	37.0
10	34.945128621208895	37.0	37.0	37.0	33.0	37.0
11	35.17027345769397	37.0	37.0	37.0	33.0	37.0
12	35.120017972128956	37.0	37.0	37.0	33.0	37.0
13	34.976757906282586	37.0	37.0	37.0	33.0	37.0
14	36.33779441380174	40.0	37.0	40.0	27.0	40.0
15	36.55397959795374	40.0	37.0	40.0	33.0	40.0
16	36.54710620827846	40.0	37.0	40.0	33.0	40.0
17	36.55167877371677	40.0	37.0	40.0	33.0	40.0
18	36.53586442794559	40.0	37.0	40.0	33.0	40.0
19	36.5583325567965	40.0	37.0	40.0	33.0	40.0
20	36.488185758690335	40.0	37.0	40.0	33.0	40.0
21	36.419148270657416	40.0	37.0	40.0	27.0	40.0
22	36.340307722258196	40.0	37.0	40.0	27.0	40.0
23	36.0122492997814	40.0	37.0	40.0	27.0	40.0
24	36.355445442302525	40.0	37.0	40.0	27.0	40.0
25	35.38538049216806	37.0	33.0	40.0	27.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5395526866341349
1101	2	0.6315172003927678
1101	3	0.4819032651789463
1101	4	0.6186364807094193
1101	5	0.6284456255050088
1101	6	0.5126480308992996
1101	7	0.4646166604412443
1101	8	0.5891239145057625
1101	9	0.7840028097915734
1101	10	0.7393270096349553
1101	11	0.5052912007052086
1101	12	0.6012542790565192
1101	13	0.44598136509220154
1101	14	1.2581602447930749
1101	15	0.9813725098368948
1101	16	0.9022408074849722
1101	17	0.9077264998834664
1101	18	1.01392353507066
1101	19	0.8620800649993114
1101	20	1.0348977928848768
1101	21	1.0510080436525016
1101	22	1.0521966155954203
1101	23	1.3682090439845993
1101	24	1.2078276357983384
1101	25	1.041672288159475
1102	1	0.2651105895936361
1102	2	0.3673103168009675
1102	3	0.23152039306191696
1102	4	0.35668462344468566
1102	5	0.29576802627062193
1102	6	0.2662831386980642
1102	7	0.2722311259728656
1102	8	0.3802516822474331
1102	9	0.5034527635822954
1102	10	0.5491955803227597
1102	11	0.36624799243114836
1102	12	0.34174787926366434
1102	13	0.3627920135596554
1102	14	1.051180181275825
1102	15	0.8134822490919333
1102	16	0.5848960138925676
1102	17	0.7534984595087124
1102	18	0.6924837181793322
1102	19	0.6461650626092919
1102	20	0.751112762012248
1102	21	0.7529410118895257
1102	22	0.8457054425898107
1102	23	1.161311383390185
1102	24	1.0142122544553658
1102	25	0.7872333331491248
1103	1	0.44032129968908507
1103	2	0.583365539715377
1103	3	0.33449528421054
1103	4	0.3120323324999532
1103	5	0.4431106537025826
1103	6	0.3060179836449919
1103	7	0.34598904508310113
1103	8	0.5160768281940946
1103	9	0.6783711286746694
1103	10	0.6326933326848732
1103	11	0.3985628269903998
1103	12	0.4684685191855351
1103	13	0.4409208116102121
1103	14	1.1609481674965352
1103	15	0.7809310522509705
1103	16	0.5878498379682568
1103	17	0.6521694023547084
1103	18	0.8020758001423474
1103	19	0.7530598630783416
1103	20	0.8484212984117505
1103	21	0.9529071487659948
1103	22	0.8334475885786574
1103	23	1.2505399680102443
1103	24	0.964901504935817
1103	25	0.8943731564393147
1104	1	0.33796297200094116
1104	2	0.4617051420655329
1104	3	0.3458628085068227
1104	4	0.3942319271006056
1104	5	0.3150619214322177
1104	6	0.23549167617937172
1104	7	0.20452892209263496
1104	8	0.3582979316289183
1104	9	0.5424416228243203
1104	10	0.5753907408176389
1104	11	0.38538075839935715
1104	12	0.29872006183813227
1104	13	0.40512652673940863
1104	14	0.984663765882317
1104	15	0.6812170459406559
1104	16	0.6199797133699008
1104	17	0.6423849915148878
1104	18	0.6031781278937558
1104	19	0.7138268006262791
1104	20	0.784100395040717
1104	21	0.7335049151157378
1104	22	0.8216108764780969
1104	23	1.1394320785382348
1104	24	0.982222969750687
1104	25	0.7407621236726101
1105	1	0.21861992426234167
1105	2	0.2532800484934974
1105	3	0.22924919971688595
1105	4	0.20134613933792878
1105	5	0.25619148289537463
1105	6	0.23447597226454064
1105	7	0.20502485189754083
1105	8	0.30804774496094467
1105	9	-1.389849636353361
1105	10	0.02983355773066876
1105	11	0.1402523760589034
1105	12	0.23631840471844612
1105	13	0.19125330702822652
1105	14	-1.5250563789450524
1105	15	-0.11446689680444422
1105	16	0.055674558635836036
1105	17	0.2266896860260914
1105	18	0.17729313123830082
1105	19	0.30996196014507404
1105	20	0.38634326733919266
1105	21	0.5872455747737888
1105	22	0.5483605763413735
1105	23	0.8961572834365938
1105	24	0.6840127209050095
1105	25	0.3523380378299379
1106	1	0.10817620897434566
1106	2	0.10922424288056831
1106	3	0.13371142552795234
1106	4	-0.89809930408056
1106	5	-0.11629774274652505
1106	6	-0.11424358155027647
1106	7	0.05588093613749123
1106	8	0.10390996335596014
1106	9	0.3555003733304787
1106	10	0.3981553534840714
1106	11	0.25352103327175257
1106	12	0.16106949361252987
1106	13	0.20425474774701513
1106	14	0.6580649789749771
1106	15	0.4224797103449802
1106	16	0.3021351455954573
1106	17	0.23363105260666828
1106	18	0.17904433742054948
1106	19	0.1854763292183037
1106	20	0.35495030249651904
1106	21	0.5210248285823553
1106	22	0.5831315437491114
1106	23	0.7454768052000702
1106	24	0.4210775233317463
1106	25	0.35132211067018204
1107	1	0.25384837071137767
1107	2	0.3526293061839212
1107	3	0.1690713816351419
1107	4	0.3460415941590682
1107	5	0.3388775921178748
1107	6	0.2042330471679321
1107	7	0.18400690799116148
1107	8	0.32644375049704877
1107	9	0.3729635888198004
1107	10	0.42868960888375796
1107	11	0.23698661642576013
1107	12	0.2877951921652695
1107	13	0.3408412133369225
1107	14	0.7520315505855777
1107	15	0.5109648262502375
1107	16	0.5205385445868345
1107	17	0.5744689589118579
1107	18	0.5698875019867558
1107	19	0.43846075415268615
1107	20	0.5722560596008748
1107	21	0.6114106370022014
1107	22	0.5770531869765847
1107	23	0.8616023641114126
1107	24	0.7556376770337678
1107	25	0.7102703040957152
1108	1	0.29610232274266934
1108	2	0.26485206119660276
1108	3	0.27373572818951786
1108	4	0.4209404893441686
1108	5	0.3581337769215125
1108	6	0.16096857708559753
1108	7	0.17715471109887204
1108	8	0.1932964865746385
1108	9	0.04829165126351853
1108	10	0.4084192710720558
1108	11	0.2031481957176524
1108	12	0.10270075749649976
1108	13	0.23010709438840848
1108	14	-1.2136480939883256
1108	15	-0.11676014199689888
1108	16	0.21114917798718125
1108	17	0.3081538329772542
1108	18	0.45558173485482456
1108	19	0.42413841767501737
1108	20	0.5577653034480292
1108	21	0.5712573872143096
1108	22	0.5584022225164702
1108	23	0.868974662770384
1108	24	0.7133449521276205
1108	25	0.5846189273708902
1109	1	0.2652022492402182
1109	2	0.1724112527716457
1109	3	0.2572441823881988
1109	4	0.3329402533089265
1109	5	0.3527187090890038
1109	6	0.19152933221702284
1109	7	0.17587139343510216
1109	8	0.32442114775213327
1109	9	0.3870721424548407
1109	10	0.4092593496862449
1109	11	0.22566041117901392
1109	12	0.21198847510654417
1109	13	0.1818316534445401
1109	14	0.648684279902831
1109	15	0.31409520381881606
1109	16	0.3471086654988511
1109	17	0.3660867777166743
1109	18	0.2830649077805347
1109	19	0.23928658858606866
1109	20	0.5500787860191068
1109	21	0.4865167681275935
1109	22	0.516536108903324
1109	23	0.926427178033336
1109	24	0.7311323186600305
1109	25	0.6136642965747825
1110	1	0.24775956776373675
1110	2	0.08306970696426674
1110	3	0.2602649750397781
1110	4	0.424003257900587
1110	5	0.340819778564061
1110	6	0.14262517456929658
1110	7	0.15728190019461863
1110	8	0.14042377786203986
1110	9	0.30290448839372175
1110	10	0.28872413776602457
1110	11	0.09729320304509059
1110	12	0.029854467476575053
1110	13	0.06385842672774089
1110	14	0.4471774828780326
1110	15	0.22416315200212011
1110	16	0.14023822047631285
1110	17	0.22279998431048398
1110	18	0.28367720266174956
1110	19	0.2556129351383518
1110	20	0.3344425950890155
1110	21	0.39721438712793145
1110	22	0.4304975078481945
1110	23	0.6041941716985662
1110	24	0.5228334613898298
1110	25	0.5439812777734687
1111	1	0.15002861336594364
1111	2	0.09011749404476532
1111	3	0.24880445284974329
1111	4	0.38656698058215255
1111	5	0.2956011274491317
1111	6	0.2694322578906494
1111	7	0.22738122902137547
1111	8	0.2517942442335155
1111	9	0.23758851414955728
1111	10	0.2910165163160201
1111	11	0.2161207190756258
1111	12	0.21873635128117286
1111	13	0.11527879485346659
1111	14	-1.775134397737503
1111	15	-0.44698468863050067
1111	16	-0.08162568759856015
1111	17	0.049151473440602444
1111	18	0.173662420671036
1111	19	0.20680261462120342
1111	20	0.26928400124343455
1111	21	0.3879332892893359
1111	22	0.2443155603292766
1111	23	-1.897132630637941
1111	24	-0.3182879842634563
1111	25	0.12134968897355236
1112	1	-0.23449069852553706
1112	2	-0.35341328697152363
1112	3	-0.07447873431866014
1112	4	0.04992530581325383
1112	5	-0.5764985167020953
1112	6	-0.7209848950723625
1112	7	-0.828287476355456
1112	8	-0.5360051243443706
1112	9	-0.49680022027191484
1112	10	-0.6455928794165189
1112	11	-0.6960786458651853
1112	12	-0.6463398746564977
1112	13	-0.6971463609724466
1112	14	-0.4419234584565075
1112	15	-0.7461248127716544
1112	16	-0.7797095338568241
1112	17	-0.7682980085685429
1112	18	-0.844344085795484
1112	19	-0.7148424601628989
1112	20	-0.7017900528411616
1112	21	-1.6452375772241865
1112	22	-1.4773980018669732
1112	23	-1.1217177281147812
1112	24	-1.540395097407
1112	25	-1.9176277020619708
1113	1	0.10921469445479914
1113	2	-0.11703010309891937
1113	3	-0.0854435520960628
1113	4	0.1618105639579568
1113	5	0.009971108416202412
1113	6	-0.022281005279864985
1113	7	-0.0513382739348458
1113	8	-0.00620132690417563
1113	9	0.07604426239311834
1113	10	-0.11797003909701687
1113	11	-0.2029890196409454
1113	12	-0.08415067383759123
1113	13	-0.22326502827725392
1113	14	-0.09627666501359045
1113	15	-0.20314365124283285
1113	16	-0.07974086880838627
1113	17	-0.08223713613484307
1113	18	-0.07920876393214371
1113	19	-0.23050224521657015
1113	20	-0.7131677864661086
1113	21	-0.5978301789976257
1113	22	-0.16018897994331383
1113	23	-0.28365952264775274
1113	24	-0.10342868936491101
1113	25	-1.309002359395528
1114	1	-0.9434464953510187
1114	2	-0.7269449724631727
1114	3	-0.6037655893643858
1114	4	-1.7759359951439961
1114	5	-0.8663973718481657
1114	6	-0.7119380329116822
1114	7	-0.6209893650869986
1114	8	-0.5359655968199135
1114	9	-0.6363966138413915
1114	10	-0.6901911391412412
1114	11	-0.7018399725913369
1114	12	-0.6732258126636523
1114	13	-0.7749965805402468
1114	14	-0.7762862177513696
1114	15	-1.4420751038690653
1114	16	-0.9816374128478671
1114	17	-0.9704480398484989
1114	18	-0.9224728983962507
1114	19	-1.019726617911168
1114	20	-2.1954418944057252
1114	21	-6.774279171582087
1114	22	-7.70013883281155
1114	23	-7.998022231440036
1114	24	-5.950002490270304
1114	25	-7.466116762439505
1115	1	-0.8831378086090425
1115	2	-0.8275569251106418
1115	3	-0.6986624004355626
1115	4	-0.5348917099984973
1115	5	-0.5712603452581462
1115	6	-0.80227791619145
1115	7	-0.91164529224109
1115	8	-0.6587361590248051
1115	9	-2.7062916479948385
1115	10	-1.4421164963215034
1115	11	-1.0315601738789937
1115	12	-0.8873576641168981
1115	13	-1.1499143424118898
1115	14	-0.9051139410733029
1115	15	-1.7588819144037018
1115	16	-1.2641002148818075
1115	17	-2.790170183371579
1115	18	-2.5924695781834046
1115	19	-1.747398681458101
1115	20	-1.4986373171448477
1115	21	-1.0395204235537747
1115	22	-0.6398977580366747
1115	23	-0.16972910792356544
1115	24	-1.1996328022891376
1115	25	-0.8510901957870729
1116	1	-1.7191582691143736
1116	2	-0.9481127540065302
1116	3	-0.7656455793631807
1116	4	-0.573985220701573
1116	5	-0.541326410502613
1116	6	-0.5958510455672084
1116	7	-0.9711244352946977
1116	8	-1.8961006893869694
1116	9	-1.031602413909603
1116	10	-2.6156501930539733
1116	11	-1.2958772203762194
1116	12	-0.944710090817658
1116	13	-1.0565184973731903
1116	14	-1.4469453437927413
1116	15	-1.440538227315173
1116	16	-1.00044373128474
1116	17	-0.9179451933058544
1116	18	-1.4546774659381256
1116	19	-1.1397049214493506
1116	20	-1.3465825566870677
1116	21	-0.8132739451495965
1116	22	-0.5081754542715586
1116	23	-0.36589142345069803
1116	24	-0.4776559434813237
1116	25	-0.4979747600438955
1201	1	0.22272540058359525
1201	2	0.3503154082038904
1201	3	0.3362432146175678
1201	4	0.41006777664431837
1201	5	0.3995976104622869
1201	6	0.2767560298032734
1201	7	0.2931728624219687
1201	8	0.41159519616213913
1201	9	0.6074260765728212
1201	10	0.5386768928678336
1201	11	0.40396666687291116
1201	12	0.3420362961656025
1201	13	0.3205122645970633
1201	14	1.0177295764762206
1201	15	0.7452855616945655
1201	16	0.7811084722514678
1201	17	0.7518552964908096
1201	18	0.570467018778146
1201	19	0.602989323496665
1201	20	0.7206290349401456
1201	21	0.7187421497911757
1201	22	0.8554422136802202
1201	23	0.6703326386522761
1201	24	0.6967937921866749
1201	25	0.7612135823263131
1202	1	-0.042404808366839575
1202	2	0.18585876621915887
1202	3	0.1457185083625241
1202	4	0.1825584154270885
1202	5	0.14626775263804603
1202	6	0.05550869619639087
1202	7	0.06180127936499247
1202	8	0.2686098419408296
1202	9	0.29831167869437536
1202	10	0.4099894754163671
1202	11	0.22589209891069828
1202	12	0.2781480412661139
1202	13	0.3329690545819375
1202	14	0.6163579975765501
1202	15	0.5824528471764552
1202	16	0.49933369272388006
1202	17	0.2899041390340358
1202	18	0.3561214808371034
1202	19	0.41672020351968087
1202	20	0.5278381387784634
1202	21	0.50259878420961
1202	22	0.5269166817839022
1202	23	0.7871732262793856
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2211	6	0.2321170403440007
2211	7	0.1239022009170796
2211	8	0.3228774936087362
2211	9	0.4473328680658071
2211	10	0.4143597515866091
2211	11	0.18572827151091076
2211	12	0.20279594623537633
2211	13	0.18760737861907018
2211	14	0.43986619118765447
2211	15	0.29650596355851633
2211	16	0.20679831091245404
2211	17	0.24845810237225407
2211	18	0.20726903608287017
2211	19	0.23925050131627046
2211	20	0.23908520081086237
2211	21	0.29483176302861125
2211	22	0.2998777982185672
2211	23	0.435942933633676
2211	24	0.5581324547412336
2211	25	0.4367514205284877
2212	1	-0.35899803800354846
2212	2	-0.34919731231518014
2212	3	-0.2514792149221563
2212	4	-0.5425575270801559
2212	5	-0.2139022597058755
2212	6	-0.49844324905990334
2212	7	-0.5310746830761914
2212	8	-0.33535819680981405
2212	9	-0.1988235358321404
2212	10	-0.47288910311438315
2212	11	-0.45851017244809356
2212	12	-0.624543709251725
2212	13	-0.6149107411239427
2212	14	-0.41597820867269775
2212	15	-0.680763785605393
2212	16	-0.46815768781097944
2212	17	-0.4559281720156676
2212	18	-0.5665238113734077
2212	19	-0.6306458438463665
2212	20	-0.6439576417129729
2212	21	-0.5001655663808293
2212	22	-0.5277500250567186
2212	23	-0.1586443064946721
2212	24	-0.302031279720957
2212	25	-1.0603793122514347
2213	1	-0.019623723755437084
2213	2	-1.092136273852116
2213	3	-0.11378543013315934
2213	4	0.07997783410068138
2213	5	0.04069321969657125
2213	6	0.13398513775029386
2213	7	0.09550585311874471
2213	8	0.3199982723912669
2213	9	0.24671953356630638
2213	10	-0.17685774844121482
2213	11	0.061887985582856686
2213	12	0.15570762021335582
2213	13	-0.22437936171606054
2213	14	0.12289777848836536
2213	15	0.15250174227365676
2213	16	0.07727337702225157
2213	17	0.20483694321679025
2213	18	0.17202210667976203
2213	19	0.21449272525145346
2213	20	0.3143884803964312
2213	21	-0.18050199839934322
2213	22	-0.7143325056272403
2213	23	-0.044142119966579685
2213	24	0.18833728403292582
2213	25	-0.3393835167400283
2214	1	-0.22273998193000466
2214	2	-0.3502531028498197
2214	3	-0.44245541954052214
2214	4	-0.5579053261653186
2214	5	-0.2504290210338951
2214	6	-0.2103617931503976
2214	7	-0.4385368157812337
2214	8	-0.11938465666315068
2214	9	-0.1156596438548263
2214	10	-1.8619285989847754
2214	11	-0.7243312000710631
2214	12	-0.4080675072519213
2214	13	-0.22961300009944097
2214	14	-0.7946986324417011
2214	15	-0.576491836598926
2214	16	-0.6887652662554444
2214	17	-0.48935234564005725
2214	18	-0.4969605596038491
2214	19	-1.3347196867463964
2214	20	-2.3394374666461317
2214	21	-1.7478778105107793
2214	22	-1.9073504556435097
2214	23	-2.453056696794782
2214	24	-1.5053071073956446
2214	25	-1.8786396934718752
2215	1	-0.1880907454299745
2215	2	-0.26746425821134423
2215	3	-0.49443841050301884
2215	4	-0.21699456606295797
2215	5	-0.15321826589169518
2215	6	-0.1489832277479266
2215	7	-0.14685079749305174
2215	8	-0.1865848521177753
2215	9	-0.3767544651089949
2215	10	-0.3897061973892306
2215	11	-0.4710676562836298
2215	12	-0.3015127662246897
2215	13	-0.2807740867459998
2215	14	-0.0874223825422007
2215	15	-0.4342558750900878
2215	16	-0.307500121874547
2215	17	-0.17881138464675672
2215	18	-0.20903426127000557
2215	19	-2.1041673833670913
2215	20	-2.260525391922627
2215	21	-3.2601780263808564
2215	22	-2.3055242278944448
2215	23	-3.036097949259876
2215	24	-2.0042493646170314
2215	25	-1.2949398531409315
2216	1	-1.3887682183203651
2216	2	-1.02487796959025
2216	3	-0.8206014684952443
2216	4	-0.8834549209350797
2216	5	-1.8440674666215884
2216	6	-1.4669090843972654
2216	7	-0.9926634204485438
2216	8	-5.02348253354544
2216	9	-3.9503575837830276
2216	10	-1.8590942146338918
2216	11	-1.0933901073627865
2216	12	-1.062173586664528
2216	13	-1.6799418206109138
2216	14	-3.171816998992341
2216	15	-1.8189300816410636
2216	16	-2.904732747134716
2216	17	-2.56177349534709
2216	18	-2.5137495302039667
2216	19	-1.4648925178459464
2216	20	-0.8013936893632518
2216	21	-0.681017408490888
2216	22	-0.6316967896929455
2216	23	-0.8525198285662867
2216	24	-0.5099154151202612
2216	25	-0.26853167119531207
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	35.0
8	200.0
9	473.0
10	989.0
11	1930.0
12	3190.0
13	5210.0
14	8022.0
15	12584.0
16	16561.0
17	18450.0
18	18340.0
19	18534.0
20	18852.0
21	19459.0
22	20146.0
23	20658.0
24	22029.0
25	23456.0
26	24398.0
27	27054.0
28	30996.0
29	36694.0
30	44658.0
31	54717.0
32	75295.0
33	110784.0
34	167604.0
35	277509.0
36	505370.0
37	1785465.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.7629454680175	19.572806680060985	11.40719920964011	24.257048642281404
2	12.878870176025966	23.02474621285649	36.92627424861137	27.170109362506167
3	21.003714216934846	28.378718929089715	27.625397490669933	22.992169363305507
4	12.983718554898935	19.325411325327025	34.14380899318982	33.54706112658422
5	11.545609354078353	40.23732483142469	34.974943372883516	13.242122441613443
6	31.92084828641538	33.99105130010198	18.230245608453423	15.857854805029223
7	24.037098126872223	34.093608312772396	22.756849508169672	19.11244405218571
8	25.94174469158518	36.41223865980147	21.636223346638374	16.009793301974984
9	26.102154957256385	18.031170224826138	20.716957905714164	35.14971691220331
10	15.842674555117265	32.18178019569973	32.41263489523547	19.56291035394753
11	29.077900317365728	27.82615456844612	20.072922299112005	23.02302281507615
12	22.418796765551768	31.33816140249246	28.861518025427934	17.381523806527834
13	28.005023817727537	22.95633629307661	23.334754888562237	25.703885000633615
14	20.666530746578182	26.829735160597895	26.249492896394766	26.254241196429163
15	29.508107689417578	26.19864380683759	21.83628874705119	22.45695975669364
16	19.060671494998154	29.914356668949853	30.51577121733546	20.50920061871653
17	24.52444101768747	30.336948937858672	27.28058640279847	17.858023641655397
18	24.500915843317784	26.92899869775746	28.815855544858515	19.754229914066237
19	22.391735627937067	31.468715547186722	29.062824125390062	17.07672469948615
20	22.92755801199413	28.324857670593484	27.616920224308295	21.130664093104087
21	19.26394846789832	30.255715105032476	30.08535123144033	20.394985195628873
22	21.333199090355325	29.74013588834548	23.632585285128226	25.29407973617097
23	18.036340696929493	30.720419463321463	27.70058173983782	23.542658099911225
24	20.63347352751593	34.51646658428329	25.134318135144344	19.715741753056435
25	21.748876456130404	27.552189873188045	23.14534578142703	27.553587889254523
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8854.0
1	8854.0
2	10896.0
3	12938.0
4	12938.0
5	12938.0
6	15872.0
7	18806.0
8	18806.0
9	18806.0
10	20716.5
11	22627.0
12	22627.0
13	22627.0
14	27762.5
15	32898.0
16	32898.0
17	32898.0
18	45272.0
19	57646.0
20	57646.0
21	57646.0
22	76770.5
23	95895.0
24	95895.0
25	95895.0
26	119943.0
27	143991.0
28	143991.0
29	143991.0
30	169245.0
31	194499.0
32	194499.0
33	194499.0
34	214934.5
35	235370.0
36	235370.0
37	235370.0
38	252777.5
39	270185.0
40	270185.0
41	270185.0
42	302914.0
43	335643.0
44	335643.0
45	335643.0
46	557352.5
47	779062.0
48	779062.0
49	779062.0
50	669101.0
51	559140.0
52	559140.0
53	559140.0
54	411628.5
55	264117.0
56	264117.0
57	264117.0
58	206330.0
59	148543.0
60	148543.0
61	148543.0
62	122164.0
63	95785.0
64	95785.0
65	95785.0
66	74490.5
67	53196.0
68	53196.0
69	53196.0
70	39005.0
71	24814.0
72	24814.0
73	24814.0
74	17592.5
75	10371.0
76	10371.0
77	10371.0
78	6991.5
79	3612.0
80	3612.0
81	3612.0
82	2396.5
83	1181.0
84	1181.0
85	1181.0
86	775.5
87	370.0
88	370.0
89	370.0
90	233.0
91	96.0
92	96.0
93	96.0
94	55.0
95	14.0
96	14.0
97	14.0
98	11.5
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03175392665495828
2	0.014838283483625361
3	0.021218745381584265
4	0.02181227672092928
5	0.02056586090830475
6	0.006944316670336669
7	1.1870626786900288E-4
8	3.561188036070087E-4
9	0.001068356410821026
10	2.967656696725072E-4
11	0.0014244752144280347
12	0.001810270585002294
13	0.0033534520672993313
14	0.0010386798438537753
15	0.00246315505828181
16	0.0031457160985285766
17	0.0029973332636923228
18	0.002670891027052565
19	5.045016384432622E-4
20	4.1547193754151013E-4
21	0.08164023572690673
22	0.07813840082477115
23	0.07991899484280619
24	0.001483828348362536
25	0.23014177683102935
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3369662.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.91928152526443
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.1247621206654	52.19961469664307
2	7.273918500702288	8.426002668680106
3	1.2412204204752955	2.156717849052472
4	0.43734478879872835	1.0132278378416344
5	0.22590217183003236	0.6542045743696152
6	0.134441356457166	0.46720480639686157
7	0.08902083665373933	0.3609216029833755
8	0.06861916621100135	0.31794982446431197
9	0.05344264472695029	0.278582362685543
>10	0.30280194903759844	3.308302975153654
>50	0.02316302217404308	0.9199794399396308
>100	0.017787258126718917	1.9917260835658428
>500	0.002853883751821747	1.105642535622893
>1k	0.002853883751780016	3.351369052529686
>5k	7.783319323036408E-4	3.661462278288129
>10k+	0.001089664705225097	19.78709141178318
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	117270	3.480171008249492	No Hit
CCCATGTACTCTGCGTTGATACCAC	111723	3.3155550912821528	No Hit
GAGTACATGGGAAGCAGTGGTATCA	56639	1.6808510764581137	No Hit
CATGTACTCTGCGTTGATACCACTG	45598	1.3531921005726983	No Hit
GCGTTGATACCACTGCTTCCCATGT	37685	1.1183614261608434	No Hit
GCTTCCCATGTACTCTGCGTTGATA	33851	1.0045814684084042	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	30134	0.8942736689911333	No Hit
GTATCAACGCAGAGTACATGGGAAG	29969	0.8893770354415369	No Hit
ACTCTGCGTTGATACCACTGCTTCC	24979	0.7412909662749558	No Hit
TATCAACGCAGAGTACATGGGAAGC	23712	0.7036907559274491	No Hit
GGTATCAACGCAGAGTACATGGGAA	20612	0.6116933983289718	No Hit
CAGTGGTATCAACGCAGAGTACATG	16523	0.4903459159998837	No Hit
GTGGTATCAACGCAGAGTACATGGG	15840	0.4700768207612514	No Hit
GCAGTGGTATCAACGCAGAGTACAT	13103	0.3888520569718862	No Hit
ATACCACTGCTTCCCATGTACTCTG	12972	0.38496442669917635	No Hit
GTACTCTGCGTTGATACCACTGCTT	12146	0.36045158238422725	No Hit
CATGGGAAGCAGTGGTATCAACGCA	11731	0.3481358070928182	No Hit
ACATGGGAAGCAGTGGTATCAACGC	11506	0.3414585795251868	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	11434	0.33932186670354475	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	10933	0.32445390665295215	No Hit
GTTGATACCACTGCTTCCCATGTAC	10033	0.29774499638242646	No Hit
TACCACTGCTTCCCATGTACTCTGC	9914	0.29421348491332366	No Hit
GTACATGGTAAGCAGTGGTATCAAC	9864	0.29272965656496114	No Hit
CTTCCCATGTACTCTGCGTTGATAC	9750	0.28934652793069454	No Hit
GATACCACTGCTTCCCATGTACTCT	9544	0.2832331551354409	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	9464	0.2808590297780608	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	9289	0.27566563055879195	No Hit
CCACTGCTTCCCATGTACTCTGCGT	9186	0.27260894416116516	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	7831	0.23239719592054042	No Hit
CCATGTACTCTGCGTTGATACCACT	7787	0.23109142697398136	No Hit
TTCCCATGTACTCTGCGTTGATACC	7320	0.21723247020027525	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	6673	0.19803173137246405	No Hit
GTACATGGGTAAGCAGTGGTATCAA	6653	0.19743820003311907	No Hit
TGATACCACTGCTTCCCATGTACTC	6632	0.19681499212680675	No Hit
ACCATGTACTCTGCGTTGATACCAC	6603	0.1959543716847565	No Hit
CCCCATGTACTCTGCGTTGATACCA	5321	0.15790901283274109	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	4608	0.13674962058509132	No Hit
ATCAACGCAGAGTACATGGGAAGCA	4547	0.13493935000008903	No Hit
GCTTACCATGTACTCTGCGTTGATA	3942	0.11698502698490235	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	3543	0.1051440767649693	No Hit
AAAAAGTACTCTGCGTTGATACCAC	3374	0.10012873694750393	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	2.967656696725072E-5	0.0	0.0	0.0	0.0
9	2.967656696725072E-5	0.0	0.0	2.967656696725072E-5	0.0
10	2.967656696725072E-5	0.0	0.0	5.935313393450144E-5	0.0
11	2.967656696725072E-5	0.0	0.0	1.1870626786900288E-4	0.0
12	2.967656696725072E-5	0.0	0.0	2.0773596877075504E-4	0.0
13	2.967656696725072E-5	0.0	0.0	2.0773596877075504E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTCC	55	0.0030798165	12.085611	8
CGCAAAA	345	0.0	11.015224	2
GAACAAA	1650	0.0	10.481507	1
GTACAAA	850	0.0	10.285021	1
GACTCCG	95	1.6526545E-4	9.995471	7
GTCCTAG	250	0.0	9.882564	1
TGGACTG	295	0.0	9.659938	5
CTTAGGC	140	1.0586155E-6	9.500631	3
TCTATAC	320	0.0	9.50063	3
AGAACCG	80	0.004521926	9.498939	5
ACTGATC	220	1.6370905E-11	9.495837	8
ACGCAAA	455	0.0	9.398043	1
GTGTAGG	355	0.0	9.368628	1
GTCTAGC	135	6.830169E-6	9.150522	1
GTGTTAG	385	0.0	9.132238	1
TCTGTCG	125	2.7702661E-5	9.116004	8
GTCTTAG	230	4.1836756E-11	9.089314	1
CTAGGAC	210	6.675691E-10	9.048221	3
CGGCGTT	105	4.515313E-4	9.043521	9
CTAGTAC	95	0.0018235063	9.000598	3
>>END_MODULE