FastQCFastQC Report
Wed 25 May 2016
SRR1294767_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294767_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3369662
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1195413.547566491832119No Hit
GTACATGGGAAGCAGTGGTATCAAC1171143.4755414638026014No Hit
GAGTACATGGGAAGCAGTGGTATCA635191.8850258571927987No Hit
CATGTACTCTGCGTTGATACCACTG510891.5161461297898722No Hit
GCGTTGATACCACTGCTTCCCATGT395201.1728179265457486No Hit
ACGCAGAGTACATGGGAAGCAGTGG371901.1036715255120544No Hit
GCTTCCCATGTACTCTGCGTTGATA371791.1033450832754146No Hit
GTATCAACGCAGAGTACATGGGAAG318730.9458812189471822No Hit
ACTCTGCGTTGATACCACTGCTTCC280840.8334367067082692No Hit
TATCAACGCAGAGTACATGGGAAGC274340.8141469381795562No Hit
GGTATCAACGCAGAGTACATGGGAA230250.6833029544209479No Hit
CAGTGGTATCAACGCAGAGTACATG199340.5915726859251759No Hit
GTGGTATCAACGCAGAGTACATGGG174290.5172328856722128No Hit
CATGGGAAGCAGTGGTATCAACGCA153130.4544372699695103No Hit
GCAGTGGTATCAACGCAGAGTACAT150790.44749295329917366No Hit
ACATGGGAAGCAGTGGTATCAACGC145080.43054763356087344No Hit
ATACCACTGCTTCCCATGTACTCTG142180.4219414291403708No Hit
GGGAAGCAGTGGTATCAACGCAGAG128860.3824122419399928No Hit
CTGCTTCCCATGTACTCTGCGTTGA124920.370719674554896No Hit
AAGCAGTGGTATCAACGCAGAGTAC116470.3456429754675691No Hit
GTACTCTGCGTTGATACCACTGCTT114050.33846124626149443No Hit
GCAGAGTACATGGGAAGCAGTGGTA111350.33044857318033677No Hit
CTTCCCATGTACTCTGCGTTGATAC109440.3247803488895919No Hit
GATACCACTGCTTCCCATGTACTCT107020.3175986196835172No Hit
TACCACTGCTTCCCATGTACTCTGC106380.3156993193976132No Hit
GTTGATACCACTGCTTCCCATGTAC102890.3053421975260427No Hit
CCACTGCTTCCCATGTACTCTGCGT101250.3004752405434135No Hit
ATGGGAAGCAGTGGTATCAACGCAG96950.28771431674749576No Hit
GTATCAACGCAGAGTACTTTTTTTT95270.2827286534969976No Hit
GTACATGGTAAGCAGTGGTATCAAC93830.2784552278537135No Hit
CCATGTACTCTGCGTTGATACCACT89260.26489303674967996No Hit
ACCATGTACTCTGCGTTGATACCAC75690.2246219353751207No Hit
TTCCCATGTACTCTGCGTTGATACC74660.22156524897749388No Hit
GTACATGGGTAAGCAGTGGTATCAA73230.21732149990117702No Hit
GGTATCAACGCAGAGTACTTTTTTT73090.21690602796363553No Hit
TATCAACGCAGAGTACTTTTTTTTT71470.2120984241149409No Hit
TGATACCACTGCTTCCCATGTACTC67620.20067294583254938No Hit
CCCCATGTACTCTGCGTTGATACCA57630.1710260554322659No Hit
GGTAAGCAGTGGTATCAACGCAGAG56560.16785066276677008No Hit
ATCAACGCAGAGTACATGGGAAGCA54810.1626572635475012No Hit
GTACTTTTTTTTTTTTTTTTTTTTT52870.15690000955585456No Hit
ACGCAGAGTACTTTTTTTTTTTTTT50230.1490653958765004No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA44340.1315858979327897No Hit
GCTTACCATGTACTCTGCGTTGATA43210.12823244586549037No Hit
GGAAGCAGTGGTATCAACGCAGAGT42850.12716408945466934No Hit
GAGTACTTTTTTTTTTTTTTTTTTT39290.11659923161432809No Hit
GAGTACATGGTAAGCAGTGGTATCA38050.11291933731038899No Hit
AAAAAGTACTCTGCGTTGATACCAC36460.10820076316259612No Hit
CTGCGTTGATACCACTGCTTCCCAT34370.10199836066644072No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGCG604.1155214E-412.658887
TACGCAG802.821709E-511.8941765
CACGTAC1553.5724952E-910.436183
CTAGACA2800.010.1950074
CTGTCGC1409.790165E-810.1721649
CAGCGTA750.002662815610.1269559
GGCGCGA750.00266379910.12650415
CGCAAAA1701.5188562E-910.0750672
CTAGTGC951.6185477E-410.0161483
CGTACAC1151.0077676E-59.9290513
GGACCAT1252.5867248E-69.8756866
TAGACAG3800.09.7657445
ACGCAAA2002.5465852E-109.5204371
CCACGTA1752.6035195E-89.2434742
CGTACTC1651.040662E-79.2269975
GTCCTAC1356.6683915E-69.16782951
AGTACTC10850.09.1206955
ACAGCGT1151.1187792E-49.0813718
CGAGGCG950.00183583628.99393412
ACTAGAC1804.1141902E-88.98671153
Produced by FastQC (version 0.11.5)