FastQCFastQC Report
Wed 25 May 2016
SRR1294766_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294766_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2881735
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC764642.6534015098543065No Hit
CCCATGTACTCTGCGTTGATACCAC713462.475800168995414No Hit
GAGTACATGGGAAGCAGTGGTATCA375971.304665418575962No Hit
CATGTACTCTGCGTTGATACCACTG298771.0367712506528186No Hit
GCTTCCCATGTACTCTGCGTTGATA232890.8081589736738458No Hit
GCGTTGATACCACTGCTTCCCATGT231160.802155645817537No Hit
ACGCAGAGTACATGGGAAGCAGTGG199020.6906256126951299No Hit
GTATCAACGCAGAGTACATGGGAAG188060.6525929691661446No Hit
ACTCTGCGTTGATACCACTGCTTCC156510.5431103137519585No Hit
TATCAACGCAGAGTACATGGGAAGC147860.5130936744704145No Hit
GGTATCAACGCAGAGTACATGGGAA132570.4600353606421132No Hit
CAGTGGTATCAACGCAGAGTACATG105940.36762575323546404No Hit
GTGGTATCAACGCAGAGTACATGGG99880.34659675507983906No Hit
GCAGTGGTATCAACGCAGAGTACAT86270.299368262522404No Hit
ATACCACTGCTTCCCATGTACTCTG77720.26969863641174496No Hit
CATGGGAAGCAGTGGTATCAACGCA76930.2669572323617543No Hit
GTACATGGTAAGCAGTGGTATCAAC76440.26525686782441826No Hit
GTACTCTGCGTTGATACCACTGCTT75310.2613356189934189No Hit
CTGCTTCCCATGTACTCTGCGTTGA73760.2559569148447029No Hit
ACATGGGAAGCAGTGGTATCAACGC73560.25526288850293316No Hit
GGGAAGCAGTGGTATCAACGCAGAG68110.23635067068970605No Hit
GTTGATACCACTGCTTCCCATGTAC62670.21747315419356744No Hit
AAGCAGTGGTATCAACGCAGAGTAC62610.21726494629103646No Hit
CTTCCCATGTACTCTGCGTTGATAC60340.20938774731194926No Hit
GCAGAGTACATGGGAAGCAGTGGTA60250.2090754354581528No Hit
TACCACTGCTTCCCATGTACTCTGC59260.2056400050663923No Hit
CCACTGCTTCCCATGTACTCTGCGT58620.20341912077272892No Hit
GATACCACTGCTTCCCATGTACTCT58310.20234337994298573No Hit
ACCATGTACTCTGCGTTGATACCAC53490.18561734510633351No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA52330.18159199232406867No Hit
CCATGTACTCTGCGTTGATACCACT50320.17461702758928213No Hit
ATGGGAAGCAGTGGTATCAACGCAG48980.16996705109942448No Hit
GTACATGGGTAAGCAGTGGTATCAA48750.1691689208063892No Hit
TTCCCATGTACTCTGCGTTGATACC43780.15192236621340963No Hit
TGATACCACTGCTTCCCATGTACTC39090.13564744849890778No Hit
CCCCATGTACTCTGCGTTGATACCA37830.131275082545758No Hit
GGTAAGCAGTGGTATCAACGCAGAG35620.12360609146920172No Hit
GCTTACCATGTACTCTGCGTTGATA33950.11781097151542387No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGA701.0958142E-412.2091546
GTCTTAG1750.011.9459241
TGGACCG802.8735585E-511.8728955
GTCTAGC1451.2132659E-911.1407911
GGTATCG951.35263745E-511.00626517
GTACAAA6200.010.5752961
CGCAAAA2300.010.3260492
GGACCGT856.622115E-410.0545986
ACGCAAA3150.09.9549371
GCTAGAC1151.02758295E-59.91266253
TTAGGCT1651.0246367E-89.7869944
GTATTAC2550.09.6887621
CCTATAC1901.0531949E-99.4996363
CTAGCAC1901.0531949E-99.4996363
TACACCG900.00111592899.4983165
GACTCCG1002.7702597E-49.495357
TACACTG5250.09.4078565
CTAGACA2750.09.3264284
GTCCTAG1856.673872E-99.2456171
TCTGTCG1451.7188122E-69.1679248
Produced by FastQC (version 0.11.5)