##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294766_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2881735
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.19629459336129	33.0	33.0	33.0	27.0	33.0
2	31.18432159792625	33.0	33.0	33.0	27.0	33.0
3	31.21487124943827	33.0	33.0	33.0	27.0	33.0
4	34.64616177407014	37.0	37.0	37.0	27.0	37.0
5	35.042463307694845	37.0	37.0	37.0	33.0	37.0
6	35.09094625286503	37.0	37.0	37.0	33.0	37.0
7	35.150626272020155	37.0	37.0	37.0	33.0	37.0
8	35.031720126937415	37.0	37.0	37.0	33.0	37.0
9	34.753657779081	37.0	37.0	37.0	27.0	37.0
10	34.88424438749573	37.0	37.0	37.0	33.0	37.0
11	35.09491816561898	37.0	37.0	37.0	33.0	37.0
12	35.03724422960474	37.0	37.0	37.0	33.0	37.0
13	34.908034916465255	37.0	37.0	37.0	33.0	37.0
14	36.241923355200946	40.0	37.0	40.0	27.0	40.0
15	36.47671732480606	40.0	37.0	40.0	33.0	40.0
16	36.47527722014689	40.0	37.0	40.0	33.0	40.0
17	36.459706739169285	40.0	37.0	40.0	33.0	40.0
18	36.45185903630972	40.0	37.0	40.0	33.0	40.0
19	36.494495156563666	40.0	37.0	40.0	33.0	40.0
20	36.408485860080816	40.0	37.0	40.0	27.0	40.0
21	36.33732699224599	40.0	37.0	40.0	27.0	40.0
22	36.24708968728908	40.0	37.0	40.0	27.0	40.0
23	35.90615167598686	40.0	37.0	40.0	27.0	40.0
24	36.23180653321697	40.0	37.0	40.0	27.0	40.0
25	35.24363725325195	37.0	33.0	40.0	27.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5043163996465907
1101	2	0.6387007903928961
1101	3	0.44183671103822775
1101	4	0.6499868739846022
1101	5	0.5391059373293743
1101	6	0.4056463433677848
1101	7	0.42811839088309256
1101	8	0.48421230081380173
1101	9	0.7669301435885245
1101	10	0.742778105243552
1101	11	0.46539215753909247
1101	12	0.5112346503300245
1101	13	0.42188763388480766
1101	14	1.215991571575536
1101	15	0.9533738398573135
1101	16	0.8746154476380568
1101	17	0.9469355299998625
1101	18	0.978394265465262
1101	19	0.9484541432046711
1101	20	1.0227006884904242
1101	21	0.9660859038834033
1101	22	1.024587761921623
1101	23	1.2960025824584847
1101	24	1.089836072635265
1101	25	0.9698773250120567
1102	1	0.24075140371650505
1102	2	0.4358857061942203
1102	3	0.26999229751238474
1102	4	0.38972563362502655
1102	5	0.4033038132180238
1102	6	0.30830673886197246
1102	7	0.3357876868948608
1102	8	0.4111617638445253
1102	9	0.5143999804826862
1102	10	0.6323998802310413
1102	11	0.37445744856438523
1102	12	0.44271796936761376
1102	13	0.4840771400860291
1102	14	1.1151278853027051
1102	15	0.8480692375366203
1102	16	0.703157200725073
1102	17	0.8373312088767051
1102	18	0.8278493507948141
1102	19	0.7013048029348568
1102	20	0.8275562922364657
1102	21	0.8548379103385102
1102	22	0.9325418355000679
1102	23	1.323768070244597
1102	24	1.029552268880046
1102	25	0.8414875417565995
1103	1	0.3096278591762882
1103	2	0.47210908941315566
1103	3	0.30659068404554546
1103	4	0.28049329652721156
1103	5	0.42292385325541915
1103	6	0.27075417370117805
1103	7	0.18927776589799095
1103	8	0.2454720586063388
1103	9	0.5184005450516054
1103	10	0.43534754599416914
1103	11	0.35123470945571
1103	12	0.31237256464266494
1103	13	0.3123040301098783
1103	14	1.0564814630994661
1103	15	0.7715496683808212
1103	16	0.5371579034196827
1103	17	0.6261765590658186
1103	18	0.6070984582567576
1103	19	0.7429798643094969
1103	20	0.9386285588044458
1103	21	0.8895301623286684
1103	22	0.7304487073484793
1103	23	1.2120181939623507
1103	24	0.9015642092903988
1103	25	0.8451615263859082
1104	1	0.1904452079703738
1104	2	0.3504065676817376
1104	3	0.2634613970481787
1104	4	0.2821840523992307
1104	5	0.3513088013997887
1104	6	0.13233940901758956
1104	7	0.1912973841080543
1104	8	0.31269039458167214
1104	9	0.5366815692568423
1104	10	0.4925481690655431
1104	11	0.2794593704405486
1104	12	0.25354795898426374
1104	13	0.23712387907089294
1104	14	0.7105146867963796
1104	15	0.5552309655902761
1104	16	0.4696640299076762
1104	17	0.5811929451048528
1104	18	0.4672340721401582
1104	19	0.5399576460071387
1104	20	0.6437292715271639
1104	21	0.6461639005395483
1104	22	0.759414708174468
1104	23	1.1474737605633862
1104	24	0.8932799433750986
1104	25	0.7068796827614321
1105	1	0.21877247117587828
1105	2	0.22506437910309884
1105	3	0.30651509642552455
1105	4	0.2716795006535193
1105	5	0.2837178429997209
1105	6	0.18969956653485553
1105	7	0.2047944918706719
1105	8	0.23233176394809618
1105	9	-1.57013397707167
1105	10	-0.03295827003083929
1105	11	0.1371395274137157
1105	12	0.18652150546920154
1105	13	0.25390599718843987
1105	14	-1.649632628574814
1105	15	-0.21500194368427117
1105	16	-0.10772074818409294
1105	17	0.15307380056780318
1105	18	0.08980984671907777
1105	19	0.2381141634880919
1105	20	0.40541129204657267
1105	21	0.5594273848949385
1105	22	0.5184119298582601
1105	23	0.8252077835448546
1105	24	0.6083290995083033
1105	25	0.2471431637161956
1106	1	0.06001808970657052
1106	2	0.09270715920289163
1106	3	0.15264438066512298
1106	4	-1.123336017367663
1106	5	-0.24382588286843543
1106	6	-0.0464164032642671
1106	7	0.02560408132504932
1106	8	0.20317638243427893
1106	9	0.3902622501833477
1106	10	0.3621246929491093
1106	11	0.32963952405695807
1106	12	0.02903225922187147
1106	13	0.1990903909827182
1106	14	0.5206464747769886
1106	15	0.3315026980884923
1106	16	0.22907329649834196
1106	17	0.09417452088615619
1106	18	0.18042927547370624
1106	19	0.19107156295920902
1106	20	0.30017097367745293
1106	21	0.45339461408148196
1106	22	0.5219669918502987
1106	23	0.7100735066224644
1106	24	0.3534827454247491
1106	25	0.22176511303249669
1107	1	0.4376339345036868
1107	2	0.4279748451507075
1107	3	0.38996332940909895
1107	4	0.491498075020111
1107	5	0.4847762607858215
1107	6	0.3914503080490448
1107	7	0.38747823073577337
1107	8	0.4025690362974501
1107	9	0.6604399360142139
1107	10	0.541376500614831
1107	11	0.37430325545325616
1107	12	0.4182990304880647
1107	13	0.39333602177757854
1107	14	0.920112007714458
1107	15	0.699963293837996
1107	16	0.6273111494217218
1107	17	0.6196067325752495
1107	18	0.6951731307644238
1107	19	0.5850979409984802
1107	20	0.7207919969513554
1107	21	0.7625298583156379
1107	22	0.6817049965024893
1107	23	0.9931312146617515
1107	24	0.8238421980684336
1107	25	0.6574176915913981
1108	1	0.43609362031410015
1108	2	0.3177700509103225
1108	3	0.4486260782422882
1108	4	0.6139403477156407
1108	5	0.4678630276518376
1108	6	0.32427120097000284
1108	7	0.3482858114735947
1108	8	0.34771367512731644
1108	9	0.12785771638197474
1108	10	0.39129360756827936
1108	11	0.3066151703970661
1108	12	0.3382304507965941
1108	13	0.28227788396913667
1108	14	-0.9104669877450604
1108	15	0.08059654389682436
1108	16	0.40067974090894865
1108	17	0.4744947227959173
1108	18	0.530532868980842
1108	19	0.48865174929576227
1108	20	0.6486525112814405
1108	21	0.6624471119824449
1108	22	0.6874757609905799
1108	23	0.8736362959321511
1108	24	0.8723671126372423
1108	25	0.8230261441586819
1109	1	0.3196384200081006
1109	2	0.27113239334930483
1109	3	0.3291641688979965
1109	4	0.5140572816207936
1109	5	0.4783442943653782
1109	6	0.32019663014118294
1109	7	0.24904898030882094
1109	8	0.39701153748055873
1109	9	0.526765731779733
1109	10	0.4495903233756806
1109	11	0.3142942021415962
1109	12	0.2612302458951561
1109	13	0.25687380906590107
1109	14	0.6471526035869672
1109	15	0.39017954453242254
1109	16	0.36207792376793435
1109	17	0.3613918810289789
1109	18	0.44302399803052595
1109	19	0.48707526656791345
1109	20	0.5264029926098814
1109	21	0.5752106673524864
1109	22	0.7460575818402404
1109	23	1.1081638271594372
1109	24	0.7914107666164014
1109	25	0.633424358120898
1110	1	0.2588685360701284
1110	2	0.17462561666015475
1110	3	0.2558619243657638
1110	4	0.4435125298243747
1110	5	0.23467376965109565
1110	6	0.1230850503878429
1110	7	0.017006864742782568
1110	8	0.19498958673136713
1110	9	0.2498103409561594
1110	10	0.16292178025423
1110	11	0.12346893449926455
1110	12	0.08008399604661776
1110	13	0.10560698992549788
1110	14	0.43668410751833164
1110	15	0.2856750158172474
1110	16	0.20528004199307048
1110	17	0.2644597431576443
1110	18	0.2773857040202401
1110	19	0.18881197733126953
1110	20	0.32439730761195307
1110	21	0.4752257072790087
1110	22	0.5840731884749744
1110	23	0.7132334610763138
1110	24	0.608345818178833
1110	25	0.5256228323462793
1111	1	0.10817120870499508
1111	2	0.052103808645338034
1111	3	0.18241935942463883
1111	4	0.3682721608441213
1111	5	0.26663638413623403
1111	6	0.14780013173858464
1111	7	0.10460015519309707
1111	8	0.20241829536299605
1111	9	0.15182355860219587
1111	10	0.04455235786299028
1111	11	0.03701840744793827
1111	12	0.09135579018048645
1111	13	0.08421796969253847
1111	14	-1.9889105731728023
1111	15	-0.5492740128851921
1111	16	-0.14476669596613334
1111	17	0.055734232294533115
1111	18	0.0803150547214102
1111	19	0.19556754177062885
1111	20	0.12665169443391022
1111	21	0.3397394696646643
1111	22	0.22902805044051888
1111	23	-1.8791251228862365
1111	24	-0.41299351060303735
1111	25	-0.1876319726304061
1112	1	-0.2885678326525749
1112	2	-0.28922566798765104
1112	3	-0.06730723576379916
1112	4	0.041775544498463546
1112	5	-0.7358974495216302
1112	6	-0.8607577724387312
1112	7	-1.0063646855044794
1112	8	-0.7318723491761219
1112	9	-0.6061916565885213
1112	10	-0.7358672545201586
1112	11	-0.770793534549199
1112	12	-0.8246100027650343
1112	13	-0.8200972486146298
1112	14	-0.6200990132290869
1112	15	-0.9220751338830055
1112	16	-0.780252740628292
1112	17	-0.7588936349817246
1112	18	-0.9496085692226046
1112	19	-0.767532469656345
1112	20	-0.8217177685328778
1112	21	-1.7320372172281324
1112	22	-1.6023372653422143
1112	23	-1.2420138622369308
1112	24	-1.5343399083933633
1112	25	-1.8124662757176537
1113	1	-0.04095095964764539
1113	2	-0.1596834329584027
1113	3	-0.17539479747119202
1113	4	0.08828682193855997
1113	5	-0.04519075429793418
1113	6	-0.08579355245215226
1113	7	-0.09972659109273962
1113	8	-0.13509465106170637
1113	9	0.16723683133486844
1113	10	-0.08032333894911403
1113	11	-0.14823006897974977
1113	12	-0.10745751643084844
1113	13	-0.2613421738807489
1113	14	-0.04252319476413646
1113	15	-0.1415390045178242
1113	16	-0.0655342542119115
1113	17	-0.03221791104724758
1113	18	-0.042354519710556815
1113	19	-0.16450891010443058
1113	20	-0.7148248071716452
1113	21	-0.6747086850078574
1113	22	-0.18131656453801526
1113	23	-0.41474154596627244
1113	24	0.04182219995591652
1113	25	-1.1564118042434757
1114	1	-0.7768152856059096
1114	2	-0.6609804173894602
1114	3	-0.4442158632606592
1114	4	-1.416424672525281
1114	5	-0.7693716877017494
1114	6	-0.5636778659470565
1114	7	-0.6102067858171694
1114	8	-0.3696050245558524
1114	9	-0.44363880580037574
1114	10	-0.4810262255614788
1114	11	-0.5157083194639824
1114	12	-0.49542007945746036
1114	13	-0.6021343812399991
1114	14	-0.5381300618483706
1114	15	-1.1976699188895594
1114	16	-0.8008361353293338
1114	17	-0.8097239458120384
1114	18	-0.6861192127920717
1114	19	-0.7288583333351539
1114	20	-1.9820191700107515
1114	21	-6.684223903532466
1114	22	-7.587627138867379
1114	23	-7.88556850932099
1114	24	-5.73381707845877
1114	25	-6.93862741011408
1115	1	-0.9260626907356659
1115	2	-0.90596363590209
1115	3	-0.5878214274544611
1115	4	-0.5195366726961055
1115	5	-0.5285794733801268
1115	6	-0.758816184614787
1115	7	-0.976492205043769
1115	8	-0.7824811521378621
1115	9	-2.8596470247497727
1115	10	-1.587998064886257
1115	11	-1.0247466402670042
1115	12	-0.8758234951549966
1115	13	-1.328147433723636
1115	14	-1.0785482110900517
1115	15	-1.8282330935703826
1115	16	-1.253934353133431
1115	17	-3.059773931156137
1115	18	-2.7237508974825815
1115	19	-1.9287541891133628
1115	20	-1.7213681181832285
1115	21	-1.1901655594741243
1115	22	-0.7264837071357135
1115	23	-0.12061114730479972
1115	24	-1.291291780051118
1115	25	-0.972536205787577
1116	1	-1.5400907343033516
1116	2	-0.741269378752591
1116	3	-0.6496648270216454
1116	4	-0.371570189051873
1116	5	-0.4121494839322324
1116	6	-0.3832130154054809
1116	7	-0.7186827510706308
1116	8	-1.4566061320173205
1116	9	-0.7779191618198666
1116	10	-2.21656476672662
1116	11	-0.995150660798906
1116	12	-0.6852935647273668
1116	13	-0.689225358356552
1116	14	-1.165748862101438
1116	15	-1.2144536505070462
1116	16	-0.8420802226092263
1116	17	-0.7191912401898151
1116	18	-1.0980019661070486
1116	19	-0.6981253550318414
1116	20	-1.1351797541176296
1116	21	-0.4471174311318791
1116	22	-0.1817839056584134
1116	23	-0.1290912860918496
1116	24	-0.1788411871657445
1116	25	-0.24517494811941987
1201	1	0.24225448178987818
1201	2	0.27054022816951573
1201	3	0.25363624223402326
1201	4	0.3979818275196436
1201	5	0.38216778593423584
1201	6	0.1925851094339066
1201	7	0.24460801843194702
1201	8	0.2965142728264283
1201	9	0.5919604541119767
1201	10	0.5508438210857562
1201	11	0.25976081989340827
1201	12	0.2723670202391304
1201	13	0.2619259716220199
1201	14	0.9530658707005983
1201	15	0.7133248737722866
1201	16	0.6155491452905366
1201	17	0.6553117763236642
1201	18	0.5620561486365787
1201	19	0.6596347380256162
1201	20	0.7603853877944076
1201	21	0.78497874899967
1201	22	0.8520619977739656
1201	23	0.6950657417127104
1201	24	0.7610062899503731
1201	25	0.7861138400254646
1202	1	-0.1966573607380866
1202	2	0.0015281662795345596
1202	3	0.007525248890008385
1202	4	0.011982330284453724
1202	5	0.029046428122505574
1202	6	-0.07934135976754675
1202	7	-0.09176459911559931
1202	8	0.15909246864676163
1202	9	0.1089914939288974
1202	10	0.2785947313291004
1202	11	0.06188409498849978
1202	12	0.09274990427566365
1202	13	0.09562881345152618
1202	14	0.37519267092828557
1202	15	0.424132819978027
1202	16	0.34118350498382455
1202	17	0.08717524494380768
1202	18	0.19500447549884825
1202	19	0.1458423546761125
1202	20	0.33466557909720507
1202	21	0.3908924610036948
1202	22	0.3994306649139716
1202	23	0.6363532661844076
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2211	5	0.31274949979486166
2211	6	0.29176216696738067
2211	7	0.24685883660681185
2211	8	0.36670054600739377
2211	9	0.47639763727360673
2211	10	0.38890474270566244
2211	11	0.3027544021557347
2211	12	0.2353977996112704
2211	13	0.20184326168816114
2211	14	0.5255624092673088
2211	15	0.36606968004628726
2211	16	0.3276738349041892
2211	17	0.40129640535336364
2211	18	0.31566716036802234
2211	19	0.31388842976144105
2211	20	0.3923020978685372
2211	21	0.42540611288082886
2211	22	0.5191263418195504
2211	23	0.6260005926918026
2211	24	0.5710275333397661
2211	25	0.753481507677435
2212	1	-0.1657245460235508
2212	2	-0.24492190077228315
2212	3	-0.08174105804842569
2212	4	-0.4324768380187862
2212	5	-0.15629861862309724
2212	6	-0.44337679871861724
2212	7	-0.44769471269056993
2212	8	-0.20477359296464925
2212	9	-0.17763007959612054
2212	10	-0.41828910695844
2212	11	-0.4603516858950556
2212	12	-0.5477868916008077
2212	13	-0.49739716197763784
2212	14	-0.19731258834933385
2212	15	-0.6215070640954181
2212	16	-0.4630839777447733
2212	17	-0.4399381584284825
2212	18	-0.31035887543955454
2212	19	-0.27263105657714704
2212	20	-0.4214030870483043
2212	21	-0.3036199356379967
2212	22	-0.4044556393550991
2212	23	0.11414179555691106
2212	24	-0.058903238042134376
2212	25	-0.9374278865681873
2213	1	-0.1232511560956624
2213	2	-1.105524063023548
2213	3	-0.2758351211146852
2213	4	0.10348931275111539
2213	5	0.05978212477470635
2213	6	-0.010096978177585925
2213	7	-0.04405775398500822
2213	8	0.03601336759236773
2213	9	0.0713195228205592
2213	10	-0.4267627620613297
2213	11	-0.08117353727044474
2213	12	-0.09688129509442689
2213	13	-0.3749776909659204
2213	14	-0.0629581521030147
2213	15	-0.18273456752059758
2213	16	-0.02001985675808271
2213	17	0.0875059607187012
2213	18	0.09144709171457777
2213	19	0.08313449886293967
2213	20	0.05444627921929168
2213	21	-0.6161972036905041
2213	22	-0.9449329005990563
2213	23	-0.10759989652235902
2213	24	0.06617529914746711
2213	25	-0.5342746930609152
2214	1	-0.15493321897246304
2214	2	-0.32945679857302324
2214	3	-0.3209018504438852
2214	4	-0.531992611297639
2214	5	-0.28931920488683716
2214	6	-0.11535227121262182
2214	7	-0.4099988673772117
2214	8	-0.03409861170137418
2214	9	0.04056921984411588
2214	10	-1.8276508235672964
2214	11	-0.7297890142076611
2214	12	-0.4334920534410358
2214	13	-0.19466702073395936
2214	14	-0.769994674928455
2214	15	-0.5413090166723009
2214	16	-0.6167502827351967
2214	17	-0.37332859228961013
2214	18	-0.4653823566961037
2214	19	-1.2099834083828824
2214	20	-2.330281645345053
2214	21	-1.7983041060104625
2214	22	-1.8417033266452236
2214	23	-2.4950655359450877
2214	24	-1.6683286041305152
2214	25	-1.7633626851255997
2215	1	-0.22443274778859035
2215	2	-0.227152894214111
2215	3	-0.858462780985839
2215	4	-0.3955773966780072
2215	5	-0.24073469667345648
2215	6	-0.17635995797697746
2215	7	-0.12617054460701382
2215	8	-0.13177585998755603
2215	9	-0.22716817367756903
2215	10	-0.3639246830251537
2215	11	-0.6574733764464327
2215	12	-0.4442879846297174
2215	13	-0.28279429581295545
2215	14	-0.19619526991358072
2215	15	-0.6002476519020732
2215	16	-0.39674076666874925
2215	17	-0.2627869156141358
2215	18	-0.26551068500963027
2215	19	-2.247391804694715
2215	20	-2.25926601312716
2215	21	-3.3142959942567316
2215	22	-2.272665096079251
2215	23	-3.3373463430553443
2215	24	-2.2562662046035484
2215	25	-1.644493711197768
2216	1	-1.4412132673203004
2216	2	-1.1721974367660444
2216	3	-0.7705105558291514
2216	4	-0.8860443051095572
2216	5	-1.9145529394106546
2216	6	-1.4574441552255877
2216	7	-1.0638900053662113
2216	8	-5.05877554464206
2216	9	-4.02735028570406
2216	10	-1.9693543412694652
2216	11	-0.9373582753387808
2216	12	-1.0040552202322175
2216	13	-1.887839429637701
2216	14	-3.4313720604847475
2216	15	-2.037170668181787
2216	16	-3.1089267022594314
2216	17	-2.8462314739333436
2216	18	-2.8287514512991976
2216	19	-1.7024548737947924
2216	20	-0.8459089631843142
2216	21	-0.7309180526744825
2216	22	-0.7139528882665616
2216	23	-0.935580605343965
2216	24	-0.5146585338242531
2216	25	-0.40038948950988384
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	3.0
7	40.0
8	219.0
9	545.0
10	1113.0
11	2084.0
12	3422.0
13	5236.0
14	7807.0
15	11470.0
16	14219.0
17	15415.0
18	15744.0
19	15989.0
20	16309.0
21	17187.0
22	17765.0
23	18475.0
24	19557.0
25	20622.0
26	21817.0
27	24468.0
28	27269.0
29	32358.0
30	39489.0
31	48358.0
32	66193.0
33	96769.0
34	144729.0
35	237629.0
36	430925.0
37	1508510.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.522415015799105	19.85203024514563	11.476264544706979	24.149290194348282
2	13.675545611008321	22.66197574645708	37.33828108107733	26.324197561457268
3	20.515671954168045	27.71006138555166	28.229648464805873	23.54461819547442
4	12.920809932105346	18.769066194251394	35.016023499512855	33.2941003741304
5	11.835143907028177	39.9633685521415	35.17660252171803	13.024885019112286
6	31.706033903575253	35.04319152244044	17.97577304704635	15.275001526937961
7	24.656725181306083	33.7348515422512	22.786459101463045	18.821964174979673
8	25.502528692873643	36.68155820504795	21.54358186725594	16.272331234822467
9	25.63181016892689	17.638875393862	21.010125897033717	35.71918854017739
10	15.413310754303838	31.877830331085843	33.00552098912806	19.703337925482263
11	30.12092134248161	27.109027007594104	20.970965829986937	21.799085819937343
12	21.88674544184463	30.511228950381074	29.526637547311434	18.075388060462856
13	27.701606298600527	23.21939190521006	24.11575149844395	24.96325029774546
14	20.601579059143926	25.948185039747152	26.781889679621447	26.668346221487475
15	27.756249047864113	27.531310739329395	22.30883453708758	22.403605675718914
16	19.729926781861725	30.181287680065296	29.51635913012729	20.57242640794569
17	23.59273723668805	30.377615941933428	27.51509721139705	18.514549609981476
18	23.55826773156398	27.6246115513481	28.858451133116215	19.958669583971712
19	22.15297733541866	30.89272001002179	29.059961044112182	17.894341610447366
20	22.65558714556238	28.538754553281333	27.9469884752576	20.858669825898694
21	19.861311480647718	29.82598470099	29.867590562207013	20.44511325615527
22	21.39516485562045	29.504049198130623	24.778960214401387	24.321825731847547
23	18.828092821277572	30.364350394835636	27.917265454989987	22.8902913288968
24	20.733998057394654	33.1410970688452	26.04593751529047	20.07896735846968
25	21.651586810789603	28.00999465069155	24.327145340501914	26.011273198016934
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7003.0
1	7003.0
2	8303.0
3	9603.0
4	9603.0
5	9603.0
6	11804.5
7	14006.0
8	14006.0
9	14006.0
10	15962.0
11	17918.0
12	17918.0
13	17918.0
14	23485.5
15	29053.0
16	29053.0
17	29053.0
18	40854.5
19	52656.0
20	52656.0
21	52656.0
22	70749.0
23	88842.0
24	88842.0
25	88842.0
26	111866.0
27	134890.0
28	134890.0
29	134890.0
30	158857.0
31	182824.0
32	182824.0
33	182824.0
34	203155.0
35	223486.0
36	223486.0
37	223486.0
38	238568.0
39	253650.0
40	253650.0
41	253650.0
42	277581.0
43	301512.0
44	301512.0
45	301512.0
46	442775.5
47	584039.0
48	584039.0
49	584039.0
50	506449.0
51	428859.0
52	428859.0
53	428859.0
54	329052.5
55	229246.0
56	229246.0
57	229246.0
58	184668.5
59	140091.0
60	140091.0
61	140091.0
62	115845.5
63	91600.0
64	91600.0
65	91600.0
66	71432.5
67	51265.0
68	51265.0
69	51265.0
70	38104.0
71	24943.0
72	24943.0
73	24943.0
74	17673.5
75	10404.0
76	10404.0
77	10404.0
78	7150.0
79	3896.0
80	3896.0
81	3896.0
82	2635.5
83	1375.0
84	1375.0
85	1375.0
86	897.0
87	419.0
88	419.0
89	419.0
90	274.0
91	129.0
92	129.0
93	129.0
94	73.5
95	18.0
96	18.0
97	18.0
98	13.0
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030398353769517324
2	0.0147133584455198
3	0.020855491570182547
4	0.022694661375872522
5	0.020959595521448018
6	0.005239898880362004
7	2.0820790253094055E-4
8	3.1231185379641086E-4
9	5.205197563273514E-4
10	1.7350658544245046E-4
11	9.369355613892325E-4
12	0.001110442146831683
13	0.0027414040499907175
14	6.593250246813118E-4
15	0.0019432737569554453
16	0.002498494830371287
17	0.002672001415813737
18	0.0027761053670792074
19	4.858184392388613E-4
20	7.634289759467821E-4
21	0.08095817276744739
22	0.07898019769340346
23	0.08012534115732363
24	0.0013186500493626236
25	0.22892458883276914
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2881735.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.42045784363827
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.13730889494036	59.62244474544086
2	6.5720666342172125	8.598952163787777
3	1.1259561783908432	2.2098170610660666
4	0.3753363483486661	0.9821870301731617
5	0.19961096350919394	0.6529320311690621
6	0.123647752556103	0.4853455550134292
7	0.08404346638678759	0.3848713434853047
8	0.054924991783204466	0.28745744876122453
9	0.04285399752571141	0.2523175324705964
>10	0.24599891984405015	3.009284228614937
>50	0.019314617158318816	0.8690703040825554
>100	0.012990002366271586	1.7141228627303575
>500	0.002035989666507886	0.952872710678342
>1k	0.002250304368241146	3.0378710671938016
>5k	0.0010179948332519472	4.508242865618992
>10k+	6.429441052117561E-4	12.432211049713663
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	76464	2.6534015098543065	No Hit
CCCATGTACTCTGCGTTGATACCAC	71346	2.475800168995414	No Hit
GAGTACATGGGAAGCAGTGGTATCA	37597	1.304665418575962	No Hit
CATGTACTCTGCGTTGATACCACTG	29877	1.0367712506528186	No Hit
GCTTCCCATGTACTCTGCGTTGATA	23289	0.8081589736738458	No Hit
GCGTTGATACCACTGCTTCCCATGT	23116	0.802155645817537	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	19902	0.6906256126951299	No Hit
GTATCAACGCAGAGTACATGGGAAG	18806	0.6525929691661446	No Hit
ACTCTGCGTTGATACCACTGCTTCC	15651	0.5431103137519585	No Hit
TATCAACGCAGAGTACATGGGAAGC	14786	0.5130936744704145	No Hit
GGTATCAACGCAGAGTACATGGGAA	13257	0.4600353606421132	No Hit
CAGTGGTATCAACGCAGAGTACATG	10594	0.36762575323546404	No Hit
GTGGTATCAACGCAGAGTACATGGG	9988	0.34659675507983906	No Hit
GCAGTGGTATCAACGCAGAGTACAT	8627	0.299368262522404	No Hit
ATACCACTGCTTCCCATGTACTCTG	7772	0.26969863641174496	No Hit
CATGGGAAGCAGTGGTATCAACGCA	7693	0.2669572323617543	No Hit
GTACATGGTAAGCAGTGGTATCAAC	7644	0.26525686782441826	No Hit
GTACTCTGCGTTGATACCACTGCTT	7531	0.2613356189934189	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	7376	0.2559569148447029	No Hit
ACATGGGAAGCAGTGGTATCAACGC	7356	0.25526288850293316	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	6811	0.23635067068970605	No Hit
GTTGATACCACTGCTTCCCATGTAC	6267	0.21747315419356744	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	6261	0.21726494629103646	No Hit
CTTCCCATGTACTCTGCGTTGATAC	6034	0.20938774731194926	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	6025	0.2090754354581528	No Hit
TACCACTGCTTCCCATGTACTCTGC	5926	0.2056400050663923	No Hit
CCACTGCTTCCCATGTACTCTGCGT	5862	0.20341912077272892	No Hit
GATACCACTGCTTCCCATGTACTCT	5831	0.20234337994298573	No Hit
ACCATGTACTCTGCGTTGATACCAC	5349	0.18561734510633351	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	5233	0.18159199232406867	No Hit
CCATGTACTCTGCGTTGATACCACT	5032	0.17461702758928213	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	4898	0.16996705109942448	No Hit
GTACATGGGTAAGCAGTGGTATCAA	4875	0.1691689208063892	No Hit
TTCCCATGTACTCTGCGTTGATACC	4378	0.15192236621340963	No Hit
TGATACCACTGCTTCCCATGTACTC	3909	0.13564744849890778	No Hit
CCCCATGTACTCTGCGTTGATACCA	3783	0.131275082545758	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	3562	0.12360609146920172	No Hit
GCTTACCATGTACTCTGCGTTGATA	3395	0.11781097151542387	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	3.4701317088490094E-5	0.0
8	0.0	0.0	0.0	3.4701317088490094E-5	0.0
9	0.0	0.0	0.0	3.4701317088490094E-5	0.0
10	0.0	0.0	0.0	6.940263417698019E-5	0.0
11	0.0	0.0	0.0	6.940263417698019E-5	0.0
12	0.0	0.0	0.0	6.940263417698019E-5	0.0
13	0.0	0.0	0.0	6.940263417698019E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACCGA	70	1.0958142E-4	12.209154	6
GTCTTAG	175	0.0	11.945924	1
TGGACCG	80	2.8735585E-5	11.872895	5
GTCTAGC	145	1.2132659E-9	11.140791	1
GGTATCG	95	1.35263745E-5	11.006265	17
GTACAAA	620	0.0	10.575296	1
CGCAAAA	230	0.0	10.326049	2
GGACCGT	85	6.622115E-4	10.054598	6
ACGCAAA	315	0.0	9.954937	1
GCTAGAC	115	1.02758295E-5	9.9126625	3
TTAGGCT	165	1.0246367E-8	9.786994	4
GTATTAC	255	0.0	9.688762	1
CCTATAC	190	1.0531949E-9	9.499636	3
CTAGCAC	190	1.0531949E-9	9.499636	3
TACACCG	90	0.0011159289	9.498316	5
GACTCCG	100	2.7702597E-4	9.49535	7
TACACTG	525	0.0	9.407856	5
CTAGACA	275	0.0	9.326428	4
GTCCTAG	185	6.673872E-9	9.245617	1
TCTGTCG	145	1.7188122E-6	9.167924	8
>>END_MODULE