FastQCFastQC Report
Wed 25 May 2016
SRR1294766_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294766_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2881735
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC765612.6567675376118904No Hit
GTACATGGGAAGCAGTGGTATCAAC754882.61953302437594No Hit
GAGTACATGGGAAGCAGTGGTATCA407271.413280541062936No Hit
CATGTACTCTGCGTTGATACCACTG335421.1639515777821348No Hit
GCTTCCCATGTACTCTGCGTTGATA256410.8897764714659745No Hit
GCGTTGATACCACTGCTTCCCATGT252190.8751325156546317No Hit
ACGCAGAGTACATGGGAAGCAGTGG245760.8528195687667325No Hit
GTATCAACGCAGAGTACATGGGAAG198000.6870860783521038No Hit
ACTCTGCGTTGATACCACTGCTTCC177510.6159830796377876No Hit
TATCAACGCAGAGTACATGGGAAGC172940.6001245777283477No Hit
GGTATCAACGCAGAGTACATGGGAA148990.5170149233014139No Hit
CAGTGGTATCAACGCAGAGTACATG128230.4449749890257084No Hit
GTGGTATCAACGCAGAGTACATGGG111680.38754430924425737No Hit
GCAGTGGTATCAACGCAGAGTACAT99700.34597213137224625No Hit
CATGGGAAGCAGTGGTATCAACGCA98710.3425367009804857No Hit
ACATGGGAAGCAGTGGTATCAACGC91980.31918271457993186No Hit
GTATCAACGCAGAGTACTTTTTTTT84220.29225449251926355No Hit
ATACCACTGCTTCCCATGTACTCTG83630.29020711481104267No Hit
GGGAAGCAGTGGTATCAACGCAGAG82100.28489781329650365No Hit
CTGCTTCCCATGTACTCTGCGTTGA80390.27896388807437184No Hit
AAGCAGTGGTATCAACGCAGAGTAC78670.2729952615351516No Hit
GTACATGGTAAGCAGTGGTATCAAC74490.2584901109921627No Hit
GTACTCTGCGTTGATACCACTGCTT73950.25661623986938426No Hit
GCAGAGTACATGGGAAGCAGTGGTA71230.24717748162131495No Hit
CTTCCCATGTACTCTGCGTTGATAC67720.23499731932325493No Hit
GGTATCAACGCAGAGTACTTTTTTT66970.23239472054161817No Hit
GTTGATACCACTGCTTCCCATGTAC66390.23038204415048572No Hit
GATACCACTGCTTCCCATGTACTCT65540.22743243219796408No Hit
CCACTGCTTCCCATGTACTCTGCGT64230.22288655965937187No Hit
TACCACTGCTTCCCATGTACTCTGC64030.22219253331760205No Hit
TATCAACGCAGAGTACTTTTTTTTT61460.2132742948258601No Hit
ATGGGAAGCAGTGGTATCAACGCAG61200.21237206058155939No Hit
CCATGTACTCTGCGTTGATACCACT57380.19911615745375616No Hit
ACCATGTACTCTGCGTTGATACCAC56630.1965135586721194No Hit
GTACATGGGTAAGCAGTGGTATCAA53780.1866236833018997No Hit
TTCCCATGTACTCTGCGTTGATACC45290.15716226509377165No Hit
ACGCAGAGTACTTTTTTTTTTTTTT42620.14789701343114478No Hit
CCCCATGTACTCTGCGTTGATACCA41510.14404516723432237No Hit
TGATACCACTGCTTCCCATGTACTC41400.14366345274634898No Hit
GGTAAGCAGTGGTATCAACGCAGAG40820.14165077635521656No Hit
GTACTTTTTTTTTTTTTTTTTTTTT40130.13925638547611074No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA36200.12561876786033416No Hit
GCTTACCATGTACTCTGCGTTGATA35940.1247165336160334No Hit
ATCAACGCAGAGTACATGGGAAGCA32730.11357741083062808No Hit
GAGTACATGGTAAGCAGTGGTATCA31900.1106972015122834No Hit
GAGTACTTTTTTTTTTTTTTTTTTT31580.10958675936545172No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCACCG855.2425043E-511.193885
ACGGGTC600.00581001611.1005985
ACAGCGC803.7936648E-410.6811178
CTAGGGT1553.5743142E-910.4355854
GTCTAAG1752.4847395E-99.7919271
GTACTAG1752.4847395E-99.7919271
CTAGACT2350.09.717244
GTACGTG900.00109296719.519931
CACAGCG900.00112022469.4943277
GCTTAAC1651.03269485E-79.2314461
GTATTAG2600.09.1537791
CGCAAAA1252.7049024E-59.1343652
CTAGTAC1252.705457E-59.1342063
AGTACTC9000.09.0919175
GTACTTG3900.09.0317291
CCTATAC1804.1167368E-88.9861983
CCTACAC3100.08.900943
CTAAGAC1502.6469788E-68.8804783
CTTAGAC2054.0290615E-98.8185933
GGACTGT3250.08.7647546
Produced by FastQC (version 0.11.5)