FastQCFastQC Report
Wed 25 May 2016
SRR1294765_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294765_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3331392
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1457364.374627783220948No Hit
CCCATGTACTCTGCGTTGATACCAC1403014.211482767563829No Hit
GAGTACATGGGAAGCAGTGGTATCA701742.1064467946131824No Hit
CATGTACTCTGCGTTGATACCACTG576671.731018144967629No Hit
GCGTTGATACCACTGCTTCCCATGT461011.3838359460549823No Hit
GCTTCCCATGTACTCTGCGTTGATA393151.1801373119704917No Hit
ACGCAGAGTACATGGGAAGCAGTGG372111.1169805294603576No Hit
GTATCAACGCAGAGTACATGGGAAG358691.0766970683726202No Hit
ACTCTGCGTTGATACCACTGCTTCC306530.9201258813132768No Hit
TATCAACGCAGAGTACATGGGAAGC282790.8488643786141049No Hit
GGTATCAACGCAGAGTACATGGGAA243000.7294248170134287No Hit
CAGTGGTATCAACGCAGAGTACATG204990.6153283672410812No Hit
GTGGTATCAACGCAGAGTACATGGG179010.5373429485332257No Hit
GCAGTGGTATCAACGCAGAGTACAT166610.5001212706280137No Hit
ATACCACTGCTTCCCATGTACTCTG157310.47220501219910477No Hit
GTACTCTGCGTTGATACCACTGCTT148840.4467802047912704No Hit
CATGGGAAGCAGTGGTATCAACGCA141900.42594807215722436No Hit
CTGCTTCCCATGTACTCTGCGTTGA140260.4210252050794383No Hit
GTACATGGTAAGCAGTGGTATCAAC138770.4165526002343765No Hit
GGGAAGCAGTGGTATCAACGCAGAG133410.4004632297850268No Hit
ACATGGGAAGCAGTGGTATCAACGC131530.3948199431348817No Hit
GTTGATACCACTGCTTCCCATGTAC121780.3655528980077997No Hit
TACCACTGCTTCCCATGTACTCTGC121310.36414207634526347No Hit
AAGCAGTGGTATCAACGCAGAGTAC120240.3609302057518299No Hit
GCAGAGTACATGGGAAGCAGTGGTA119180.35774835264057786No Hit
CCACTGCTTCCCATGTACTCTGCGT116950.35105445411407604No Hit
CTTCCCATGTACTCTGCGTTGATAC112120.33655601022035236No Hit
GATACCACTGCTTCCCATGTACTCT111480.3346348913607285No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA102070.30638844062782167No Hit
CCATGTACTCTGCGTTGATACCACT97260.2919500316984612No Hit
ACCATGTACTCTGCGTTGATACCAC96720.2903290876606536No Hit
ATGGGAAGCAGTGGTATCAACGCAG92380.27730150039382934No Hit
GTACATGGGTAAGCAGTGGTATCAA87320.26211265440992837No Hit
TTCCCATGTACTCTGCGTTGATACC82140.24656359863984784No Hit
TGATACCACTGCTTCCCATGTACTC77700.23323583655120741No Hit
GGTAAGCAGTGGTATCAACGCAGAG66770.20042672852669396No Hit
CCCCATGTACTCTGCGTTGATACCA63700.19121136149693582No Hit
GCTTACCATGTACTCTGCGTTGATA53650.16104379190440513No Hit
ATCAACGCAGAGTACATGGGAAGCA52620.157951991239698No Hit
GAGTACATGGTAAGCAGTGGTATCA49180.14762597736921984No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA48970.14699561024340577No Hit
GGAAGCAGTGGTATCAACGCAGAGT42100.12637359998463105No Hit
AAAAAGTACTCTGCGTTGATACCAC38850.11661791827560371No Hit
GAACAAAAAAAAAAAAAAAAAAAAA38070.11427655466543715No Hit
TCCATGTACTCTGCGTTGATACCAC36300.10896346031928997No Hit
ACTCTGCGTTGATACCACTGCTTAC36080.10830307571129427No Hit
CTGCGTTGATACCACTGCTTCCCAT34720.10422069813459359No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCACG350.002176923716.27896111
CGCACGA500.001504871213.29448412
CGCAAAA3950.012.2642342
GTACAAA6900.011.8406451
TCACGCA658.055628E-411.6874599
AAGCGGT1950.011.68745810
GTATCGA1800.011.61920118
GGTATCG1850.011.30516817
GAACAAA22100.010.9615991
TATCGAC1951.8189894E-1210.74010219
ACGCAAA5100.010.6177051
CTAGCAC1909.094947E-1210.4981673
ACGCTGC1659.367795E-1010.3593388
GTCTTAG2151.8189894E-1210.1628471
GTACAAG3000.010.13338851
GCGTTGT750.002658061710.1291313
GGCCTAC856.596825E-410.0588791
CTATGCG856.6223653E-410.0546529
CGGTGGT2201.8189894E-129.92769913
GACTATC1151.0323443E-59.9089337
Produced by FastQC (version 0.11.5)